INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLS

Summary

Principal Investigator: M W Berns
Abstract: DESCRIPTION: (Verbatim from the application) The overall aim of this proposal is to design, build, and test an integrated optical and microfluidics system that will enable the performance of novel biochemical assays in single living cells. The device will be tested in biomedical systems relating primarily to cancer, and cell growth, and development, though it will have wide application in the areas of molecular medicine and drug development. This project is submitted in response to the PAS-99-010 to support Bioengineering Research Partnerships (BRP's). It involves a close collaboration amongst three academic research labs, each with a very different focus and expertise: (1) photonics/microscopy (Berns & Venugopalan); (2) BioMEMS/microfluidics (Li & Bachman); (3) analytical chemistry/cell biology (Albritton & Sims). The project's specific aims are: (1) the development of a laser microscope platform for single cell manipulation and analysis; (2) development of a multipurpose, modular microfluidics chip for single cell assays; (3) development of a broad range of analytes which can be assayed in single cells. Development of the microscope platform will involve, (a) basic studies to characterize and optimize the physical mechanisms of laser interaction with the cells and BioMEMS materials, and (b) development of a fluorescence module for detection of substrates and analytes in the integrated microfluidic system. Development of the microfluidic system will involve (a) basic engineering of the MEMS microfabrication process, (b) design engineering of different chip configurations, and (c) biomaterials compatibility studies. In addition, the BioMEMS microfluidic systems will be integrated with both the microscope platform and the chemical analysis systems described below. The third specific aim involves further perfection of a unique new bioassay system through the development of new enzyme assays for activation of kinases and proteases, and the transfer of these assays to the integrated device. A multidisciplinary approach is required to accomplish the tasks involved in the development and integration of this system. The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research.
Funding Period: 1999-09-30 - 2004-08-31
more information: NIH RePORT

Top Publications

  1. pmc Pulsed laser microbeam-induced cell lysis: time-resolved imaging and analysis of hydrodynamic effects
    Kaustubh R Rau
    Laser Microbeam and Medical Program, Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 91:317-29. 2006
  2. ncbi Laser nanosurgery of single microtubules reveals location-dependent depolymerization rates
    Nicole M Wakida
    University of California at Irvine, Beckman Laser Institute, Irvine, California 92612, USA
    J Biomed Opt 12:024022. 2007
  3. pmc Polarity reveals intrinsic cell chirality
    Jingsong Xu
    Departments of Cellular and Molecular Pharmacology, School of Medicine, University of California San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:9296-300. 2007
  4. ncbi Mechanisms of laser cellular microsurgery
    Pedro A Quinto-Su
    Department of Chemical Engineering and Materials Science, University of California, Irvine, California 92697, USA
    Methods Cell Biol 82:113-51. 2007

Detail Information

Publications4

  1. pmc Pulsed laser microbeam-induced cell lysis: time-resolved imaging and analysis of hydrodynamic effects
    Kaustubh R Rau
    Laser Microbeam and Medical Program, Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 91:317-29. 2006
    ..The implications for single cell lysis and microsurgery are discussed...
  2. ncbi Laser nanosurgery of single microtubules reveals location-dependent depolymerization rates
    Nicole M Wakida
    University of California at Irvine, Beckman Laser Institute, Irvine, California 92612, USA
    J Biomed Opt 12:024022. 2007
    ..704). Our results demonstrate the ability of both the picosecond and femtosecond lasers to cut individual microtubules. The differences between the two ablation results are discussed...
  3. pmc Polarity reveals intrinsic cell chirality
    Jingsong Xu
    Departments of Cellular and Molecular Pharmacology, School of Medicine, University of California San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:9296-300. 2007
    ..Such a template could help to determine left-right asymmetry and planar polarity in development...
  4. ncbi Mechanisms of laser cellular microsurgery
    Pedro A Quinto-Su
    Department of Chemical Engineering and Materials Science, University of California, Irvine, California 92697, USA
    Methods Cell Biol 82:113-51. 2007
    ..We relate this understanding of damage mechanisms to laser microbeam applications reported in the literature...