Growth inhibition of brain tumors by cord blood stem cells

Summary

Principal Investigator: JASTI RAO
Abstract: DESCRIPTION (provided by applicant): Despite the many therapeutic strategies undertaken for treatment of glioblastoma multiforme, the survival rate for patients afflicted with this aggressive cerebral malignancy remains low. Even with the combined use of several therapeutic modalities, a good prognosis is extremely rare as the remaining cancer cells inevitably infiltrate the normal brain tissue and cause tumor recurrence. We propose to study the interaction of umbilical cord blood stem cells with cancer cells that have a specific prevalence to gliomas. In the present proposal, we will study the molecular mechanisms that control invasion, migration, and angiogenesis in pre-established intracranial tumors of glioblastoma cells using mesenchymal stem cells from the human umbilical cord to chase these tumor cells and regress tumor growth. We hypothesize that: (1) the interaction of stem cells with glioma cells will initiate apoptosis and inhibit tumor growth;and (2) the interaction of stem cells with glioma cells will decrease expression of several signaling molecules and other proteins involved in cell survival, adhesion, migration and proliferation. The specific aims to address these hypotheses are as follows: Specific aim 1. Determine the effect of cord blood stem cells on the molecular mechanisms of proliferation, migration, invasion and apoptosis in glioblastoma and xenograft cell lines. Specific aim 1a: Determine the effect of cord blood stem cells on adhesion and migration of established glioblastoma cell lines and glioma xenograft cells. Specific aim 1b: Evaluate the effect of cord blood stem cells on the molecular mechanisms of proliferation in glioblastoma cell lines and xenograft cell lines. Specific aim 1c: Evaluate the effect of cord blood stem cells on the molecular mechanisms of apoptosis in glioma xenograft cells and glioblastoma cell lines. Specific aim 1d: Determine the effect of cord blood stem cells on the invasiveness of glioblastoma cell lines and glioma xenograft cells. Specific aim 2: Evaluate the in vivo effects of cord blood stem cells on pre-established intracranial tumor growth, and invasiveness and angiogenesis of human glioblastoma cell lines and glioma xenograft cells. Specific aim 2a: Determine the effect of the cord blood stem cells on pre-established intracranial tumor growth of human glioblastoma cell lines and glioma xenograft cells injected intracerebrally in nude mice. Specific aim 2b: Determine the effect of cord blood stem cells on the molecular mechanisms of cerebral angiogenesis in both in vitro and in vivo models. We anticipate that these results will substantially augment our understanding of how these stem cells chase and attach to these tumor cells;thus, the information gained should be of help in developing new therapeutic approaches to treating glioblastomas.
Funding Period: ----------------2006 - ---------------2011-
more information: NIH RePORT

Top Publications

  1. pmc Tissue inhibitor of metalloproteinase 3 suppresses tumor angiogenesis in matrix metalloproteinase 2-down-regulated lung cancer
    Chandramu Chetty
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, USA
    Cancer Res 68:4736-45. 2008
  2. pmc The homing of human cord blood stem cells to sites of inflammation: unfolding mysteries of a novel therapeutic paradigm for glioblastoma multiforme
    Kiran Kumar Velpula
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
    Cell Cycle 11:2303-13. 2012
  3. pmc Transcriptional repression of Mad-Max complex by human umbilical cord blood stem cells downregulates extracellular signal-regulated kinase in glioblastoma
    Kiran Kumar Velpula
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA
    Stem Cells Dev 21:1779-93. 2012
  4. pmc Downregulation of Focal Adhesion Kinase (FAK) by cord blood stem cells inhibits angiogenesis in glioblastoma
    Venkata Ramesh Dasari
    Department of Cancer Biology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Aging (Albany NY) 2:791-803. 2010
  5. pmc Therapeutic potential of siRNA-mediated targeting of urokinase plasminogen activator, its receptor, and matrix metalloproteinases
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
    Methods Mol Biol 487:267-81. 2009
  6. pmc uPA/uPAR downregulation inhibits radiation-induced migration, invasion and angiogenesis in IOMM-Lee meningioma cells and decreases tumor growth in vivo
    Odysseas Kargiotis
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 33:937-47. 2008
  7. pmc Antiangiogenic therapy in brain tumors
    Sajani S Lakka
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, 1 Illini Drive, Peoria, IL 61605, USA
    Expert Rev Neurother 8:1457-73. 2008
  8. pmc Simultaneous downregulation of uPAR and MMP-9 induces overexpression of the FADD-associated protein RIP and activates caspase 9-mediated apoptosis in gliomas
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 33:783-90. 2008
  9. pmc Demethylation-linked activation of urokinase plasminogen activator is involved in progression of prostate cancer
    Sai Murali Krishna Pulukuri
    Department of Cancer Biology and Pharmacology, College of Medicine, University of Illinois at Peoria, Peoria, IL 61656, USA
    Cancer Res 67:930-9. 2007
  10. pmc Matrix metalloproteinase-9 inhibition down-regulates radiation-induced nuclear factor-kappa B activity leading to apoptosis in breast tumors
    Sateesh Kunigal
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    Clin Cancer Res 14:3617-26. 2008

Scientific Experts

  • JASTI RAO
  • Christopher S Gondi
  • Dzung H Dinh
  • Meena Gujrati
  • Venkata Ramesh Dasari
  • Kiran Kumar Velpula
  • Sajani S Lakka
  • Chandramu Chetty
  • Sateesh Kunigal
  • Sai Murali Krishna Pulukuri
  • Jeffrey D Klopfenstein
  • Andrew J Tsung
  • Odysseas Kargiotis
  • Praveen Bhoopathi
  • S M Pulukuri
  • Norman Estes
  • Daniel G Spomar
  • Padmaja Tummalapalli
  • Kiranpreet Kaur
  • Bharathi Gorantla
  • Sravan K Vanamala
  • C Chetty
  • Sanjeeva Mohanam
  • Daniel Fassett
  • Venkateswara Rao Gogineni
  • O Kargiotis
  • S S Lakka
  • Joseph George
  • William C Olivero
  • Venkateswara R Gogineni
  • Venkata R Dasari
  • Krishna Kumar Veeravalli
  • Dan Fassett
  • Sreelatha Gopinath
  • B Gorantla
  • Prasanna Kumar Sodadasu
  • J A Knost
  • D H Dinh
  • Roger Geiss
  • Athanassios P Kyritsis
  • Sai MuraliKrishna Pulukuri
  • A P Kyritsis
  • C S Gondi
  • Pushpa Joseph
  • A J Tsung
  • Liang Li
  • M Gujrati
  • Venkateswara Gogineni
  • Neelima Kandhukuri
  • J Patel
  • P Bhoopathi
  • Kay L Saving
  • N Estes
  • Daniel Spomar
  • Christopher A Sloffer
  • Jitendra Patel
  • Craig Cady
  • S Patibandla

Detail Information

Publications40

  1. pmc Tissue inhibitor of metalloproteinase 3 suppresses tumor angiogenesis in matrix metalloproteinase 2-down-regulated lung cancer
    Chandramu Chetty
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, USA
    Cancer Res 68:4736-45. 2008
    ..These experiments provide the first evidence that inhibition of p-AKT and induction of p-ERK1/2 are crucial events in the induction of TIMP-3-mediated endothelial apoptosis in MMP-2 inhibited lung tumors...
  2. pmc The homing of human cord blood stem cells to sites of inflammation: unfolding mysteries of a novel therapeutic paradigm for glioblastoma multiforme
    Kiran Kumar Velpula
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
    Cell Cycle 11:2303-13. 2012
    ..Our results indicate that multiple cytokines are involved in recruitment of hUCBSC toward glioma stem cells, and that hUCBSC are a potential candidate for glioma therapy...
  3. pmc Transcriptional repression of Mad-Max complex by human umbilical cord blood stem cells downregulates extracellular signal-regulated kinase in glioblastoma
    Kiran Kumar Velpula
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA
    Stem Cells Dev 21:1779-93. 2012
    ....
  4. pmc Downregulation of Focal Adhesion Kinase (FAK) by cord blood stem cells inhibits angiogenesis in glioblastoma
    Venkata Ramesh Dasari
    Department of Cancer Biology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Aging (Albany NY) 2:791-803. 2010
    ..Taken together, our results suggest that hUCBSC have the potential to inhibit the angiogenesis of glioma cells both in vitro and in vivo...
  5. pmc Therapeutic potential of siRNA-mediated targeting of urokinase plasminogen activator, its receptor, and matrix metalloproteinases
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
    Methods Mol Biol 487:267-81. 2009
    ..In this chapter we discuss the therapeutic potential of siRNA-mediated targeting of the uPAR-uPA system and MMPs as therapeutic agents for the treatment of cancer...
  6. pmc uPA/uPAR downregulation inhibits radiation-induced migration, invasion and angiogenesis in IOMM-Lee meningioma cells and decreases tumor growth in vivo
    Odysseas Kargiotis
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 33:937-47. 2008
    ..Thus, the specific targeting of proteases via RNA interference could augment the therapeutic effect of radiation and prevent the adverse effects resulting from tumor cells that receive sublethal doses of radiation within the tumor mass...
  7. pmc Antiangiogenic therapy in brain tumors
    Sajani S Lakka
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, 1 Illini Drive, Peoria, IL 61605, USA
    Expert Rev Neurother 8:1457-73. 2008
    ....
  8. pmc Simultaneous downregulation of uPAR and MMP-9 induces overexpression of the FADD-associated protein RIP and activates caspase 9-mediated apoptosis in gliomas
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 33:783-90. 2008
    ..Based on our results we conclude that the simultaneous downregulation of uPAR and MMP-9 induces apoptosome-mediated apoptosis...
  9. pmc Demethylation-linked activation of urokinase plasminogen activator is involved in progression of prostate cancer
    Sai Murali Krishna Pulukuri
    Department of Cancer Biology and Pharmacology, College of Medicine, University of Illinois at Peoria, Peoria, IL 61656, USA
    Cancer Res 67:930-9. 2007
    ..Collectively, these findings strongly suggest that DNA demethylation is a common mechanism underlying the abnormal expression of uPA and is a critical contributing factor to the malignant progression of human prostate tumors...
  10. pmc Matrix metalloproteinase-9 inhibition down-regulates radiation-induced nuclear factor-kappa B activity leading to apoptosis in breast tumors
    Sateesh Kunigal
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    Clin Cancer Res 14:3617-26. 2008
    ..In the present study, we show that the modulation of MMP-9 expression, using adenoviral-mediated transfer of the antisense MMP-9 gene (MMP-9 adenoviral construct, Ad-MMP-9), affects breast cancer sensitivity to radiation...
  11. pmc Adenovirus-mediated transfer of siRNA against MMP-2 mRNA results in impaired invasion and tumor-induced angiogenesis, induces apoptosis in vitro and inhibits tumor growth in vivo in glioblastoma
    O Kargiotis
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Oncogene 27:4830-40. 2008
    ..Thus, specific targeting of MMP-2 may provide a novel, efficient approach for the treatment of gliomas and improve the poor outcomes of patients with these brain tumors...
  12. pmc Matrix metalloproteinase-1 promotes prostate tumor growth and metastasis
    Sai Murali Krishna Pulukuri
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA
    Int J Oncol 32:757-65. 2008
    ..Collectively, our findings suggest that MMP-1 plays an important role in prostate cancer progression during the invasive and metastatic stages of the disease...
  13. pmc Downregulation of matrix metalloproteinase-2 (MMP-2) utilizing adenovirus-mediated transfer of small interfering RNA (siRNA) in a novel spinal metastatic melanoma model
    Andrew J Tsung
    Department of Neurosurgery, University of Illinois College of Medicine, Peoria, IL 61605, USA
    Int J Oncol 32:557-64. 2008
    ..This novel experimental model revealed that adenoviral-mediated transfer of RNA interference against MMP-2 results in the retention of neurological function and significantly inhibited tumor growth...
  14. pmc Blockade of tumor growth due to matrix metalloproteinase-9 inhibition is mediated by sequential activation of beta1-integrin, ERK, and NF-kappaB
    Praveen Bhoopathi
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    J Biol Chem 283:1545-52. 2008
    ..In addition, ERK activation plays an active role in this process and functions upstream of NF-kappaB activation to initiate the apoptotic signal...
  15. pmc RNAi-mediated abrogation of cathepsin B and MMP-9 gene expression in a malignant meningioma cell line leads to decreased tumor growth, invasion and angiogenesis
    Padmaja Tummalapalli
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    Int J Oncol 31:1039-50. 2007
    ..Furthermore, these observations demonstrate that the simultaneous RNAi-mediated targeting of cathepsin B and MMP-9 has potential application for the treatment of human meningiomas...
  16. pmc Epigenetic inactivation of the tissue inhibitor of metalloproteinase-2 (TIMP-2) gene in human prostate tumors
    S M Pulukuri
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA
    Oncogene 26:5229-37. 2007
    ..These results suggest that the downregulation of the TIMP-2 gene is associated with promoter methylation and that this may play an important role in prostate cancer progression during the invasive and metastatic stages of the disease...
  17. pmc Axonal remyelination by cord blood stem cells after spinal cord injury
    Venkata Ramesh Dasari
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois 61605, USA
    J Neurotrauma 24:391-410. 2007
    ..Therefore, hUCB facilitate functional recovery after moderate SCI and may prove to be a useful therapeutic strategy to repair the injured spinal cord...
  18. pmc MMP-9 short interfering RNA induced senescence resulting in inhibition of medulloblastoma growth via p16(INK4a) and mitogen-activated protein kinase pathway
    Jasti S Rao
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois 61605, USA
    Cancer Res 67:4956-64. 2007
    ..These studies validate the usefulness of targeting MMP-9 and provide a novel perspective in the treatment of medulloblastoma...
  19. pmc RNA interference-mediated targeting of urokinase plasminogen activator receptor and matrix metalloproteinase-9 gene expression in the IOMM-lee malignant meningioma cell line inhibits tumor growth, tumor cell invasion and angiogenesis
    Padmaja Tummalapalli
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 31:5-17. 2007
    ..In addition, the present study indicated that targeting both the proteins simultaneously augmented the therapeutic treatment of human meningiomas...
  20. pmc Down-regulation of uPAR and uPA activates caspase-mediated apoptosis and inhibits the PI3K/AKT pathway
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 31:19-27. 2007
    ..Our studies provide evidence for the presence of a feedback response of the uPAR-uPA system indicative of the multifaceted role of uPAR, and also the therapeutic potential of simultaneously targeting uPAR and uPA in cancer patients...
  21. pmc Mesenchymal stem cells from rat bone marrow downregulate caspase-3-mediated apoptotic pathway after spinal cord injury in rats
    Venkata Ramesh Dasari
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL 61656, USA
    Neurochem Res 32:2080-93. 2007
    ..The ability of rMSC to incorporate into the spinal cord, differentiate and to improve locomotor recovery hold promise for a potential cure after SCI...
  22. pmc MMP-2 siRNA induced Fas/CD95-mediated extrinsic II apoptotic pathway in the A549 lung adenocarcinoma cell line
    C Chetty
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA
    Oncogene 26:7675-83. 2007
    ..This is the first report, to our knowledge, showing that MMP-2 inhibition upregulates TIMP-3 levels, which in turn, promotes apoptosis in lung cancer...
  23. pmc Inhibition of histone deacetylase activity promotes invasion of human cancer cells through activation of urokinase plasminogen activator
    Sai Murali Krishna Pulukuri
    Department of Cancer Biology, University of Illinois College of Medicine, Peoria, Illinois 61656, USA
    J Biol Chem 282:35594-603. 2007
    ....
  24. pmc Umbilical cord blood stem cell mediated downregulation of fas improves functional recovery of rats after spinal cord injury
    Venkata Ramesh Dasari
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL 61656, USA
    Neurochem Res 33:134-49. 2008
    ..Taken together, our results indicate that hUCB-mediated downregulation of Fas and caspases leads to functional recovery of hind limbs of rats after SCI...
  25. pmc RNAi-mediated downregulation of urokinase plasminogen activator receptor and matrix metalloprotease-9 in human breast cancer cells results in decreased tumor invasion, angiogenesis and growth
    Sateesh Kunigal
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA
    Int J Cancer 121:2307-16. 2007
    ..In conclusion, our results provide evidence that the simultaneous downregulation of uPAR and MMP-9 using RNAi technology may provide an effective tool for breast cancer therapy...
  26. pmc Small interfering RNA directed reversal of urokinase plasminogen activator demethylation inhibits prostate tumor growth and metastasis
    Sai Murali Krishna Pulukuri
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    Cancer Res 67:6637-46. 2007
    ....
  27. pmc Intraperitoneal injection of a hairpin RNA-expressing plasmid targeting urokinase-type plasminogen activator (uPA) receptor and uPA retards angiogenesis and inhibits intracranial tumor growth in nude mice
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois 61605, USA
    Clin Cancer Res 13:4051-60. 2007
    ..The purpose of this study was to evaluate the therapeutic potential of using plasmid-expressed RNA interference (RNAi) targeting urokinase-type plasminogen activator (uPA) receptor (uPAR) and uPA to treat human glioma...
  28. pmc Restoration of tissue factor pathway inhibitor-2 in a human glioblastoma cell line triggers caspase-mediated pathway and apoptosis
    Joseph George
    Department of Cancer Biology, University of Illinois, College of Medicine at Peoria, Peoria, Illinois, USA
    Clin Cancer Res 13:3507-17. 2007
    ..Tissue factor pathway inhibitor-2 (TFPI-2) is a protease inhibitor that is abundant in the extracellular matrix and highly expressed in noninvasive cells but absent or undetectable in highly invasive human glioblastoma cells...
  29. pmc Inhibition of matrix metalloproteinase-2 enhances radiosensitivity by abrogating radiation-induced FoxM1-mediated G2/M arrest in A549 lung cancer cells
    Chandramu Chetty
    Program of Cancer Biology, Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA
    Int J Cancer 124:2468-77. 2009
    ....
  30. pmc Stat3-siRNA induces Fas-mediated apoptosis in vitro and in vivo in breast cancer
    Sateesh Kunigal
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 34:1209-20. 2009
    ..Thus, targeting Stat3 signaling using siRNA may serve as a novel therapeutic strategy for the treatment of breast cancers expressing constitutively activated Stat3...
  31. pmc Concepts in in vivo siRNA delivery for cancer therapy
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    J Cell Physiol 220:285-91. 2009
    ..This review presents the early and intriguing successes of using siRNAs for in vivo gene silencing and its use as a possible cancer therapeutics...
  32. pmc Frequent loss of cystatin E/M expression implicated in the progression of prostate cancer
    S M Pulukuri
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, 61605, USA
    Oncogene 28:2829-38. 2009
    ....
  33. pmc Effect of human umbilical cord blood cells on Ang-II-induced hypertrophy in mice
    Sravan K Vanamala
    Department of Cancer Biology and Pharmacology, University of Illinois, College of Medicine at Peoria, Peoria, IL 61656, USA
    Biochem Biophys Res Commun 386:386-91. 2009
    ..Our findings support hUCB as a feasible model for experimentation in stem cell therapy and emphasize the relevance of the hUCB in reversing heart failure conditions...
  34. pmc MMP-2 downregulation mediates differential regulation of cell death via ErbB-2 in glioma xenografts
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 35:257-63. 2009
    ..Our results suggest that MMP-2 may play a role in a hitherto unknown signaling pathway mediated via ErbB-2 in certain cancer cell types...
  35. pmc Induction of apoptosis in glioma cells requires cell-to-cell contact with human umbilical cord blood stem cells
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 36:1165-73. 2010
    ..Taken together, these results demonstrate that hUCB have therapeutic potential with possible clinical implications...
  36. pmc Human umbilical cord blood stem cells show PDGF-D-dependent glioma cell tropism in vitro and in vivo
    Christopher S Gondi
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois 61605, USA
    Neuro Oncol 12:453-65. 2010
    ..Our results demonstrate that hUCB are capable of inducing apoptosis in human glioma cells and also show that glioma tropism and hUCB tropism toward glioma cells are partially dependent on the PDGF/PGGFR system...
  37. pmc Upregulation of PTEN in glioma cells by cord blood mesenchymal stem cells inhibits migration via downregulation of the PI3K/Akt pathway
    Venkata Ramesh Dasari
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, USA
    PLoS ONE 5:e10350. 2010
    ..Based on this capability, new therapeutic approaches have been developed using mesenchymal stem cells to cure glioblastoma. However, molecular mechanisms of interactions between glioma cells and stem cells are still unknown...
  38. pmc Cord blood stem cell-mediated induction of apoptosis in glioma downregulates X-linked inhibitor of apoptosis protein (XIAP)
    Venkata Ramesh Dasari
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America
    PLoS ONE 5:e11813. 2010
    ..We hypothesized that downregulation of XIAP by human umbilical cord blood mesenchymal stem cells (hUCBSC) in glioma cells would cause them to undergo apoptotic death...
  39. pmc RNAi-mediated downregulation of radiation-induced MMP-9 leads to apoptosis via activation of ERK and Akt in IOMM-Lee cells
    Venkateswara Rao Gogineni
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
    Int J Oncol 34:209-18. 2009
    ....
  40. pmc Regulation of glioblastoma progression by cord blood stem cells is mediated by downregulation of cyclin D1
    Kiran Kumar Velpula
    Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America
    PLoS ONE 6:e18017. 2011
    ..Rapid induction of cyclin D1 and its associated binding with cyclin-dependent kinases, in the presence or absence of mitogenic signals, often is considered a rate-limiting step during cell cycle progression through the G(1) phase...