EXPRESSION AND IN VIVO ROLE OF TROPHININ IN MICE

Summary

Principal Investigator: Michiko Fukuda
Abstract: DESCRIPTION (adapted from investigator's abstract): The goal of this proposal is to define the in vivo role of trophinin in embryo implantation using the mouse as a model. The specific aims are: 1) to define the in vivo role of trophinin by analyzing trophinin gene knockout mice; 2) to define the expression and detailed localization of trophinin in mouse blastocysts and endometrium by determining the localization of trophinin in mouse blastocyst and endometrial epithelium at peri-implantation periods by immunoelectron microscopy, 3) to define the interaction between trophinin and tastin by yeast two-hybrid assays, and 4) to define the promoter activity of the human trophinin gene in order to understand the temporal and spatial expression of the trophinin in the cells involved in implantation.
Funding Period: 1997-01-01 - 2002-12-31
more information: NIH RePORT

Top Publications

  1. pmc The Bysl gene product, bystin, is essential for survival of mouse embryos
    Rui Aoki
    Cancer Research Center, Burnham Institute for Medical Research, La Jolla, CA, USA
    FEBS Lett 580:6062-8. 2006
  2. pmc Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation
    Kazuhiro Sugihara
    Cancer Research Center and Inflammation and Infectious Disease Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 104:3799-804. 2007
  3. ncbi The role of trophinin, an adhesion molecule unique to human trophoblasts, in progression of colorectal cancer
    Oi Harada
    Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
    Int J Cancer 121:1072-8. 2007
  4. ncbi Induction of trophinin in human endometrial surface epithelia by CGbeta and IL-1beta
    Kazuhiro Sugihara
    Cancer Research Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    FEBS Lett 582:197-202. 2008

Detail Information

Publications4

  1. pmc The Bysl gene product, bystin, is essential for survival of mouse embryos
    Rui Aoki
    Cancer Research Center, Burnham Institute for Medical Research, La Jolla, CA, USA
    FEBS Lett 580:6062-8. 2006
    ..After implantation bystin re-appeared in the epiblast. Targeted disruption of the mouse bystin gene, Bysl, resulted in embryonic lethality shortly after implantation, indicating that bystin is essential for survival of mouse embryos...
  2. pmc Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation
    Kazuhiro Sugihara
    Cancer Research Center and Inflammation and Infectious Disease Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 104:3799-804. 2007
    ..These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation...
  3. ncbi The role of trophinin, an adhesion molecule unique to human trophoblasts, in progression of colorectal cancer
    Oi Harada
    Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
    Int J Cancer 121:1072-8. 2007
    ..6% of trophinin-positive patients (n = 32). These results suggest that trophinin promotes invasion through a mechanism involving HMGB1/RAGE...
  4. ncbi Induction of trophinin in human endometrial surface epithelia by CGbeta and IL-1beta
    Kazuhiro Sugihara
    Cancer Research Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    FEBS Lett 582:197-202. 2008
    ..These results provide a mechanism for trophinin-mediated adhesion of human blastocyst to endometrium by a spatially and temporally restricted paracrine effect of hCG derived from the blastocyst...