Intracellular Assembly of the Coronavirus, IBV

Summary

Principal Investigator: Carolyn Machamer
Affiliation: Johns Hopkins University
Country: USA
Abstract: All enveloped viruses exploit the cellular secretory pathway for biosynthesis of their membrane proteins. The best studied enveloped viruses assemble by budding from the plasma membrane, where their membrane proteins accumulate. Assembly of enveloped viruses at intracellular membranes is less well understood, although these viruses must also accumulate their membrane proteins at the budding site. Intracellular assembly of the avian coronavirus infectious bronchitis virus (IBV) in the cis Golgi network will be studied. Coronaviruses are ubiquitous in vertebrates, and in humans cause mild respiratory disease (responsible for about 20 percent of common colds). Coronaviruses are conveniently studied in cell culture systems, and are thus an ideal model for intracellular virus assembly. Understanding the intracellular assembly of enveloped viruses is important because several virus families that cause significant human disease assembly at intracellular membranes. These include Bun yaviridae and Flaviviridae. The long term goals of the proposed experiments are to elucidate the mechanism and advantages of intracellular assembly of enveloped viruses, and to identify unique strategies to interfere with assembly and infection by this subset of viruses. Specifically, the experiments are designed to test the following hypotheses: (1) the IBV RNA 3 proteins (3a, 3b, and E) play important roles in virus assembly and infection; (2) the IBV E protein has an additional function in infected cells to slow membrane traffic, allowing S to accumulate at the budding site and possibly preventing virus antigen presentation to the immune system; and (3) distinct envelope lipids (derived from the cis Golgi network) provide an advantage for the virus during subsequent rounds of infection by promoting fusion with susceptible cells.
Funding Period: 2002-04-01 - 2006-03-31
more information: NIH RePORT

Top Publications

  1. ncbi Infectious bronchitis virus 3a protein localizes to a novel domain of the smooth endoplasmic reticulum
    Amanda R Pendleton
    Department of Cell Biology, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Virol 79:6142-51. 2005
  2. ncbi Contribution of trafficking signals in the cytoplasmic tail of the infectious bronchitis virus spike protein to virus infection
    Soonjeon Youn
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Virol 79:13209-17. 2005
  3. ncbi Differential localization and turnover of infectious bronchitis virus 3b protein in mammalian versus avian cells
    Amanda R Pendleton
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Virology 345:337-45. 2006
  4. ncbi Transcriptional regulation of RNA3 of infectious bronchitis virus
    Soonjeon Youn
    Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Adv Exp Med Biol 581:109-12. 2006
  5. ncbi The transmembrane domain of the infectious bronchitis virus E protein is required for efficient virus release
    Carolyn E Machamer
    Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Adv Exp Med Biol 581:193-8. 2006
  6. ncbi The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein
    Corrin E McBride
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:2418-28. 2007
  7. ncbi Generating antibodies to the gene 3 proteins of infectious bronchitis virus
    Amanda R Pendleton
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 454:163-89. 2008

Detail Information

Publications7

  1. ncbi Infectious bronchitis virus 3a protein localizes to a novel domain of the smooth endoplasmic reticulum
    Amanda R Pendleton
    Department of Cell Biology, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Virol 79:6142-51. 2005
    ..Our results suggest that IBV 3a is partially targeted to a novel domain of the smooth ER...
  2. ncbi Contribution of trafficking signals in the cytoplasmic tail of the infectious bronchitis virus spike protein to virus infection
    Soonjeon Youn
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N. Wolfe St, Baltimore, MD 21205, USA
    J Virol 79:13209-17. 2005
    ..Our results suggest that the endocytosis signal of IBV S is essential for productive virus infection...
  3. ncbi Differential localization and turnover of infectious bronchitis virus 3b protein in mammalian versus avian cells
    Amanda R Pendleton
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Virology 345:337-45. 2006
    ..Our results highlight the importance of using cells derived from the natural host when studying coronavirus non-structural proteins...
  4. ncbi Transcriptional regulation of RNA3 of infectious bronchitis virus
    Soonjeon Youn
    Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Adv Exp Med Biol 581:109-12. 2006
  5. ncbi The transmembrane domain of the infectious bronchitis virus E protein is required for efficient virus release
    Carolyn E Machamer
    Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Adv Exp Med Biol 581:193-8. 2006
  6. ncbi The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein
    Corrin E McBride
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:2418-28. 2007
    ..Thus, the cycling of SARS S through the ER-Golgi system may be required for its incorporation into assembling virions in the ERGIC...
  7. ncbi Generating antibodies to the gene 3 proteins of infectious bronchitis virus
    Amanda R Pendleton
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 454:163-89. 2008
    ..Antibodies were raised in rabbits following inoculation with IBV-specific peptides and GST fusion proteins, and were screened by immunofluorescence, radioimmunoprecipitation, and immunoblotting...