Development of tissue-specific cell lines in Drosophila

Summary

Principal Investigator: AMANDA A SIMCOX
Abstract: Drosophila is one of a handful of model organisms used extensively in biomedical research. Importantly 60% of human disease genes are conserved in the fly. Genetic analysis in Drosophila has focused primarily on a whole organism approach but cell lines have also been at the forefront of research because of their utility in biochemical analysis. Moreover, the recent development of cell-based RNAi screens, which have the potential to assay the function of all genes, has further highlighted the importance of cell lines. With these developments has come the realization that the small number of poorly characterized cell lines, with ill-defined cell origins, will limit the full potential of these approaches in Drosophila. Correspondingly, there is a broad consensus in the field that efforts should be made to develop cell-type specific Drosophila cell lines. The goal of this proposal is to address these needs by 1) improving the utility of existing cell lines through genome-wide expression analysis and 2) to develop a genetic method to immortalize/transform Drosophila primary cells. This method will be targeted to specific tissues to derive cell-type specific cell lines. Preliminary results showing that expression of a highly conserved oncogene can induce cell proliferation in primary cells support this goal. The completion of this work is expected to significantly increase understanding of the mechanisms of cell immortalization and transformation in Drosophila and to generate several cell-type specific cell lines. These cell lines will be distributed to the community and the information about them disseminated on a web site. Subsequent use of the cell lines by individual investigators to study diverse biological problems is expected to impact our understanding of human disease.
Funding Period: 2009-08-14 - 2010-06-30
more information: NIH RePORT

Top Publications

  1. pmc Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes
    Amanda Simcox
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio, United States of America
    PLoS Genet 4:e1000142. 2008
  2. ncbi Progress towards Drosophila epithelial cell culture
    Amanda Simcox
    Department of Molecular Genetics, Ohio State University, Columbus, OH, USA
    Methods Mol Biol 945:1-11. 2013
  3. pmc Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling
    Jessica Leonardi
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Development 138:3569-78. 2011
  4. pmc Loss of the tumor suppressor Pten promotes proliferation of Drosophila melanogaster cells in vitro and gives rise to continuous cell lines
    Steven E Justiniano
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 7:e31417. 2012
  5. pmc Drosophila embryonic 'fibroblasts': extending mutant analysis in vitro
    AMANDA A SIMCOX
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio 43210, USA
    Fly (Austin) 2:306-9. 2008

Scientific Experts

  • AMANDA A SIMCOX
  • Steven E Justiniano
  • Jessica Leonardi
  • Sayan Mitra
  • Anne Mathew
  • Sathiya N Manivannan
  • Yi Dong Li
  • Hamed Jafar-Nejad
  • Rodrigo Fernandez-Valdivia

Detail Information

Publications5

  1. pmc Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes
    Amanda Simcox
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio, United States of America
    PLoS Genet 4:e1000142. 2008
    ..We conclude that expression of Ras(V12) is a powerful genetic mechanism to promote proliferation in Drosophila primary culture cells and serves as an efficient means to generate continuous cell lines of a given genotype...
  2. ncbi Progress towards Drosophila epithelial cell culture
    Amanda Simcox
    Department of Molecular Genetics, Ohio State University, Columbus, OH, USA
    Methods Mol Biol 945:1-11. 2013
    ..It is expected that Drosophila epithelial cell lines will have great utility for in vitro analysis of epithelial biology, particularly high-throughput analyses such as RNAi screens...
  3. pmc Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling
    Jessica Leonardi
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Development 138:3569-78. 2011
    ....
  4. pmc Loss of the tumor suppressor Pten promotes proliferation of Drosophila melanogaster cells in vitro and gives rise to continuous cell lines
    Steven E Justiniano
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 7:e31417. 2012
    ..We conclude that the use of Pten mutants is a powerful means to derive new Drosophila cell lines...
  5. pmc Drosophila embryonic 'fibroblasts': extending mutant analysis in vitro
    AMANDA A SIMCOX
    Department of Molecular Genetics, Ohio State University, Columbus, Ohio 43210, USA
    Fly (Austin) 2:306-9. 2008
    ..In this addendum, we provide new data describing the genesis of seven cell lines corresponding to a rumi mutant, which demonstrate that the method can be used to derive lines and study genetic mutants in vitro...