CELL-CELL INTERACTIONS IN DEVELOPING RETINA

Summary

Principal Investigator: Nicholas Baker
Affiliation: Albert Einstein College of Medicine
Country: USA
Abstract: The aim of the research is to understand how cell-cell interactions regulate the formation of a critical cell type in the developing retina. Cell-cell interactions mediated by extracellular factors play a major role in the determination of cell type during development. They are the main factor in cell determination in both vertebrate and Drosophila retina. Drosophila can be studied by identifying genes through mutations that affect development, and characterizing how the genes function through developmental and molecular studies. Therefore the determination of R8 photoreceptor cells in Drosophila retina will be studied as a model to understand mechanisms of cell-cell interaction. In this system the behavior of individual calls can be studied, genetic techniques used" to identify and study gene products that are involved. R8 photoreceptor cells are the first cells to differentiate in the retina. The pattern of R8 photoreceptor cells is determined by a process involving lateral inhibition. Determination of cells in other parts of the Drosophila nervous system appears to be similar and requires some of the same genes. Lateral inhibition is also used to determine various cell types in other organisms. Two mutations known to affect the pattern of R8 photoreceptor cells are scabrous and Notch. scabrous encodes a putative secreted protein. Notch encodes a transmembrane protein. Notch is the Drosophila homologue of TAN-1 , a human proto-oncogene. scabrous and Notch also regulate cell determination in other parts of the Drosophila nervous system. To study cell-cell interactions in R8 photoreceptor determination, further important genes will be identified using a novel genetic screen based on properties of scabrous and Notch mutations. The roles of previously known genes will be investigated using scabrous expression as a sensitive assay for determination in their mutants. To test if Notch or other proteins is a receptor for scabrous, the nature of the scabrous protein products and Notch scabrous binding will be investigated biochemically.
Funding Period: 1992-08-01 - 1997-07-31
more information: NIH RePORT

Top Publications

  1. ncbi Extracellular signals responsible for spatially regulated proliferation in the differentiating Drosophila eye
    Lucy C Firth
    Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Dev Cell 8:541-51. 2005
  2. ncbi Notch activity opposes Ras-induced differentiation during the Second Mitotic Wave of the developing Drosophila eye
    Lihui Yang
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    BMC Dev Biol 6:8. 2006
  3. ncbi Spitz from the retina regulates genes transcribed in the second mitotic wave, peripodial epithelium, glia and plasmatocytes of the Drosophila eye imaginal disc
    Lucy C Firth
    Department of Molecular Genetics, Albert Einstein College of Medicine, NY 10461, USA
    Dev Biol 307:521-38. 2007
  4. ncbi The HLH protein Extramacrochaetae is required for R7 cell and cone cell fates in the Drosophila eye
    Abhishek Bhattacharya
    Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Dev Biol 327:288-300. 2009
  5. ncbi Retinal determination genes as targets and possible effectors of extracellular signals
    Lucy C Firth
    Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Dev Biol 327:366-75. 2009
  6. ncbi Regulation of Hh signal transduction as Drosophila eye differentiation progresses
    Nicholas E Baker
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Biol 335:356-66. 2009

Scientific Experts

Detail Information

Publications6

  1. ncbi Extracellular signals responsible for spatially regulated proliferation in the differentiating Drosophila eye
    Lucy C Firth
    Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Dev Cell 8:541-51. 2005
    ..These studies identify the specific extracellular signals that pattern the retinal cell cycles and show how differentiation can be uncoupled from cell cycle exit...
  2. ncbi Notch activity opposes Ras-induced differentiation during the Second Mitotic Wave of the developing Drosophila eye
    Lihui Yang
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    BMC Dev Biol 6:8. 2006
    ..It is not known how fate specification is limited to G1-arrested cells...
  3. ncbi Spitz from the retina regulates genes transcribed in the second mitotic wave, peripodial epithelium, glia and plasmatocytes of the Drosophila eye imaginal disc
    Lucy C Firth
    Department of Molecular Genetics, Albert Einstein College of Medicine, NY 10461, USA
    Dev Biol 307:521-38. 2007
    ..These novel targets suggest that during eye development, EGFR activity coordinates transcriptional programs in other tissues with retinal differentiation...
  4. ncbi The HLH protein Extramacrochaetae is required for R7 cell and cone cell fates in the Drosophila eye
    Abhishek Bhattacharya
    Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Dev Biol 327:288-300. 2009
    ..A model is proposed in which Extramacrochaetae acts in parallel to or as a feed-forward regulator of the E(spl)-Complex to promote Notch signaling in particular cellular contexts...
  5. ncbi Retinal determination genes as targets and possible effectors of extracellular signals
    Lucy C Firth
    Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Dev Biol 327:366-75. 2009
    ....
  6. ncbi Regulation of Hh signal transduction as Drosophila eye differentiation progresses
    Nicholas E Baker
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Biol 335:356-66. 2009
    ..A model is presented for regulation of the Cullin-3 and Cullin-1 pathways that modifies Hedgehog signaling as the morphogenetic furrow moves and the responses of retinal cells change...