Nutritional Restriction and Activity Thermogenesis

Summary

Principal Investigator: J A Levine
Affiliation: Mayo Clinic
Country: USA
Abstract: How humans adapt to negative energy balance is critical for understanding the physiology of energy storage and more broadly how mammals maintain energy stores during food scarcity. Also, understanding negative energy balance is crucial for understanding and treating obesity, which is epidemic in developed countries. During negative energy balance, fat stores diminish and energy expenditure declines. Previous studies have documented that during energy restriction, only small changes in basal metabolic rate and the thermic effect of food occur, implying that activity thermogenesis (AT) may contribute substantively to energy modulation during negative energy balance. In this proposal we will examine AT during negative energy balance in lean and obese human subjects. In Specific Aim 1 we will address the hypothesis that AT decreases during energy restriction in lean individuals. Here, we will not only ascertain how non-exercise activity thermogenesis (NEAT) changes during 1000 kcal/day negative energy balance but also tease apart how the components of AT change. Specifically, we will study the effects of negative energy balance on, sitting, standing, walking, changing posture and fidgeting thermogenesis. In Specific Aim 2 we will address the hypothesis that obesity is associated with exaggerated decline in NEAT during underfeeding. Here, we will energy restrict obese and lean subjects and measure NEAT and its components before and after underfeeding. Also, to ascertain whether differences between the obese and lean subjects relate to excess adiposity alone or to the susceptibility of an individual to obesity, we will similarly underfeed a group of post-obese subjects. Our third specific aim is incredibly exciting. Here, we want to determine whether an imposed 200 kcal/day of walking in addition to energy restriction, attenuates the decline in AT with underfeeding in lean, obese and post-obese subjects. We want to know this, in part, from a mechanistic perspective in order to gain insight into how volitional AT is impacted by imposed walking. Also, this will help us to better understand how to treat our obese patients and to define the energetic benefit of combining a walking program with energy restriction. In conclusion, this program will allow us to define for the first time how AT, NEAT and its components change during negative energy balance in lean individuals. Next, these studies will provide us with enormous insight regarding how obese patients adapt energetically during negative energy balance. Finally, we will gain fundamental information regarding the metabolic implications of combining food restriction with a walking program compatible to that advocated by statutory agencies. These studies will lead to improved understanding of the energetic adaptation that occurs during negative energy balance and how best to treat patients with obesity. KEY PERSONNEL. See instructions. Use continuation pages as needed to provide the required information in the format shown below. Start with Principal Investigator. List all other key personnel in alphabetical order, last name first. Name Organization Role on Project James Levine, M.D., Ph.D. Mayo Clinic, Rochester Principal Investigator Matthew Clark, Ph.D. Mayo Clinic, Rochester Co-Investigator Disclosure Permission Statement. Applicable to SBIR/STTR Only. See instructions. [] Yes [] No . PHS 398 (Rev. 05/01) Page 2 Form Page 2 D [] Principal Investigator/Program Director (Last, first, middle): Levine, James Andrew RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page .................................................................................................................................................. 1 2 Description,
Funding Period: 2003-02-15 - 2009-12-31
more information: NIH RePORT

Top Publications

  1. pmc Treadmill workstations: the effects of walking while working on physical activity and work performance
    Avner Ben-Ner
    Carlson School of Management, University of Minnesota Twin Cities, Minneapolis, Minnesota, United States of America
    PLoS ONE 9:e88620. 2014
  2. doi Treadmill desks: A 1-year prospective trial
    Gabriel A Koepp
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA
    Obesity (Silver Spring) 21:705-11. 2013
  3. pmc Integrated electronic platforms for weight loss
    Shelly K McCrady-Spitzer
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Expert Rev Med Devices 7:201-7. 2010
  4. pmc Spontaneous activity, economy of activity, and resistance to diet-induced obesity in rats bred for high intrinsic aerobic capacity
    Colleen M Novak
    Department of Biological Sciences, Kent State University, Kent, OH 44242, USA
    Horm Behav 58:355-67. 2010
  5. pmc A computational model to determine energy intake during weight loss
    Diana M Thomas
    Department of Mathematical Sciences, Montclair State University, Montclair, NJ, USA
    Am J Clin Nutr 92:1326-31. 2010
  6. pmc The energy expenditure of an activity-promoting video game compared to sedentary video games and TV watching
    Naim Mitre
    Division of Pediatric Endocrinology and Metabolism, Mayo Clinic College of Medicine, Rochester, MN, USA
    J Pediatr Endocrinol Metab 24:689-95. 2011
  7. doi Gait asymmetry of transfemoral amputees using mechanical and microprocessor-controlled prosthetic knees
    Kenton R Kaufman
    Mayo Clinic, Motion Analysis Laboratory, 200 First Street SW, Rochester, MN 55905, USA
    Clin Biomech (Bristol, Avon) 27:460-5. 2012
  8. pmc Measurement of daily activity in restrictive type anorexia nervosa
    Ann M Harris
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA
    Int J Eat Disord 41:280-3. 2008
  9. pmc Changing the school environment to increase physical activity in children
    Lorraine Lanningham-Foster
    Endocrine Research Unit, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Obesity (Silver Spring) 16:1849-53. 2008
  10. pmc Metabolic consequences of pregnancy-associated plasma protein-A deficiency in mice: exploring possible relationship to the longevity phenotype
    Cheryl A Conover
    Division of Endocrinology, Metabolism and Nutrition, Mayo Clinic, 200 First Street SW, 5 194 Joseph, Rochester, Minnesota 55905, USA
    J Endocrinol 198:599-605. 2008

Scientific Experts

  • J A Levine
  • Kenton Kaufman
  • COLLEEN NOVAK
  • Diana M Thomas
  • Naim Mitre
  • W G Thompson
  • CATHERINE KOTZ
  • Cheryl Conover
  • Shelly K McCrady
  • Lorraine Lanningham-Foster
  • Ann M Harris
  • Randal C Foster
  • Chinmay U Manohar
  • Avner Ben-Ner
  • Shelly K McCrady-Spitzer
  • Teresa B Jensen
  • Chinmay Manohar
  • Darla J Hamann
  • Gabriel A Koepp
  • David A McAlpine
  • Lorraine M Lanningham-Foster
  • Gabriel Koepp
  • Chimnay U Manohar
  • Carlisle F Runge
  • Ioannis T Pavlidis
  • Shelly McCrady
  • James O Hill
  • Donald E McAlpine
  • Rashmi Shirbhate
  • Donald Hensrud
  • Brian A Walker
  • Aoife B Redmond
  • Dieter Heinz
  • Michael D Jensen
  • Denny J Padgett
  • Lana J Nysse

Detail Information

Publications37

  1. pmc Treadmill workstations: the effects of walking while working on physical activity and work performance
    Avner Ben-Ner
    Carlson School of Management, University of Minnesota Twin Cities, Minneapolis, Minnesota, United States of America
    PLoS ONE 9:e88620. 2014
    ..b) Participants were outfitted with accelerometers at the start of the study. We find that daily total physical activity increased as a result of the adoption of treadmill workstations. ..
  2. doi Treadmill desks: A 1-year prospective trial
    Gabriel A Koepp
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA
    Obesity (Silver Spring) 21:705-11. 2013
    ..Sedentariness is associated with weight gain and obesity. A treadmill desk is the combination of a standing desk and a treadmill that allow employees to work while walking at low speed...
  3. pmc Integrated electronic platforms for weight loss
    Shelly K McCrady-Spitzer
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Expert Rev Med Devices 7:201-7. 2010
    ..In this article we explore the concepts and design challenges for electronic platforms that precipitate weight loss...
  4. pmc Spontaneous activity, economy of activity, and resistance to diet-induced obesity in rats bred for high intrinsic aerobic capacity
    Colleen M Novak
    Department of Biological Sciences, Kent State University, Kent, OH 44242, USA
    Horm Behav 58:355-67. 2010
    ....
  5. pmc A computational model to determine energy intake during weight loss
    Diana M Thomas
    Department of Mathematical Sciences, Montclair State University, Montclair, NJ, USA
    Am J Clin Nutr 92:1326-31. 2010
    ..Energy intake (EI) during weight loss is difficult and costly to measure accurately...
  6. pmc The energy expenditure of an activity-promoting video game compared to sedentary video games and TV watching
    Naim Mitre
    Division of Pediatric Endocrinology and Metabolism, Mayo Clinic College of Medicine, Rochester, MN, USA
    J Pediatr Endocrinol Metab 24:689-95. 2011
    ..In the present study we investigated the effect of television watching and the use of activity-promoting video games on energy expenditure in obese and lean children...
  7. doi Gait asymmetry of transfemoral amputees using mechanical and microprocessor-controlled prosthetic knees
    Kenton R Kaufman
    Mayo Clinic, Motion Analysis Laboratory, 200 First Street SW, Rochester, MN 55905, USA
    Clin Biomech (Bristol, Avon) 27:460-5. 2012
    ..The purpose of this study was to compare the gait asymmetry of active transfemoral amputees while using a passive mechanical knee joint or a microprocessor-controlled knee joint...
  8. pmc Measurement of daily activity in restrictive type anorexia nervosa
    Ann M Harris
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA
    Int J Eat Disord 41:280-3. 2008
    ..We wanted to test a daily activity detecting device named, the physical activity monitoring system (PAMS)...
  9. pmc Changing the school environment to increase physical activity in children
    Lorraine Lanningham-Foster
    Endocrine Research Unit, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Obesity (Silver Spring) 16:1849-53. 2008
    ..Children will move more in an activity-permissive environment. Strategies to increase the activity of school children may involve re-designing the school itself...
  10. pmc Metabolic consequences of pregnancy-associated plasma protein-A deficiency in mice: exploring possible relationship to the longevity phenotype
    Cheryl A Conover
    Division of Endocrinology, Metabolism and Nutrition, Mayo Clinic, 200 First Street SW, 5 194 Joseph, Rochester, Minnesota 55905, USA
    J Endocrinol 198:599-605. 2008
    ..These findings are discussed in the context of those from other long-lived mouse models...
  11. pmc Energy expenditure and activity of transfemoral amputees using mechanical and microprocessor-controlled prosthetic knees
    Kenton R Kaufman
    Motion Analysis Laboratory, Mayo Clinic, Rochester, MN 55905, USA
    Arch Phys Med Rehabil 89:1380-5. 2008
    ..To quantify the energy efficiency of locomotion and free-living physical activity energy expenditure of transfemoral amputees using a mechanical and microprocessor-controlled prosthetic knee...
  12. pmc Pedometer accuracy for children: can we recommend them for our obese population?
    Naim Mitre
    Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Pediatrics 123:e127-31. 2009
    ..Our secondary hypothesis was that the accelerometer-based step-counter provides an accurate measure of walking steps in normal and overweight children...
  13. pmc Activity-promoting video games and increased energy expenditure
    Lorraine Lanningham-Foster
    Endocrine Research Unit, Mayo Clinic, Rochester, MN, USA
    J Pediatr 154:819-23. 2009
    ..To test the hypothesis that both children and adults would expend more calories and move more while playing activity-promoting video games compared with sedentary video games...
  14. pmc Daily intraparaventricular orexin-A treatment induces weight loss in rats
    Colleen M Novak
    Endocrine Research Unit, Mayo Clinic, St Marys Hospital, Rochester, Minnesota, USA
    Obesity (Silver Spring) 17:1493-8. 2009
    ..These results support the concept that orexinergic agents have the potential to produce negative energy balance through increasing physical activity. This presents a promising, untapped potential resource for weight loss...
  15. pmc Sedentariness at work: how much do we really sit?
    Shelly K McCrady
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA
    Obesity (Silver Spring) 17:2103-5. 2009
    ..72, P < 0.001). Work days are associated with more sitting and less walking/standing time than leisure days. We suggest a need to develop approaches to free people from their chairs and render them more active...
  16. pmc Endurance capacity, not body size, determines physical activity levels: role of skeletal muscle PEPCK
    Colleen M Novak
    Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA
    PLoS ONE 4:e5869. 2009
    ..Therefore, the lean phenotype is characterized by high endurance capacity and high activity and may stem from altered skeletal muscle energetics...
  17. pmc An accelerometer-based earpiece to monitor and quantify physical activity
    Chinmay Manohar
    Endocrine Research Unit, Mayo Clinic, Rochester, MN, USA
    J Phys Act Health 6:781-9. 2009
    ..Here we examined whether the PASE could accurately and precisely detect physical activity and measure its intensity and thence predict energy expenditure...
  18. ncbi Gait and balance of transfemoral amputees using passive mechanical and microprocessor-controlled prosthetic knees
    K R Kaufman
    Motion Analysis Laboratory, Mayo Clinic, Rochester, MN 55905, USA
    Gait Posture 26:489-93. 2007
    ..These joints are more sophisticated and more expensive than mechanical ones. The literature is contradictory regarding changes in gait and balance when using these sophisticated devices...
  19. ncbi Feasibility of a walking workstation to increase daily walking
    W G Thompson
    Occupational and Aerospace Medicine, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA
    Br J Sports Med 42:225-8; discussion 228. 2008
    ..A walking workstation that allows workers to walk while they work has the potential to increase caloric expenditure. We evaluated whether employees can and will use walking workstations while performing their jobs...
  20. pmc Sensitivity of the hypothalamic paraventricular nucleus to the locomotor-activating effects of neuromedin U in obesity
    Colleen M Novak
    Mayo Clinic, Endocrine Research Unit, St Marys Hospital, Joseph 5 194, 200 1st St SW, Rochester, MN 55905, USA
    Brain Res 1169:57-68. 2007
    ..Taken together with previous studies, these results suggest that obesity may represent a state associated with decreased central sensitivity to neuropeptides such as NMU that increase physical activity and therefore energy expenditure...
  21. ncbi Caloric restriction and physical activity in zebrafish (Danio rerio)
    Colleen M Novak
    Endocrine Research Unit, Mayo Clinic and Mayo Foundation, St Mary s Hospital, Rochester, MN 55905, USA
    Neurosci Lett 383:99-104. 2005
    ..Thus, the association between negative energy balance and reductions in PA occurs across genera in biology and is associated with predictable neurological changes in brain gene expression...
  22. ncbi NEAT--non-exercise activity thermogenesis--egocentric & geocentric environmental factors vs. biological regulation
    J A Levine
    Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, MN, USA
    Acta Physiol Scand 184:309-18. 2005
    ..Deciphering the role of NEAT may lead to a better understanding of the pathogenesis, prevention and treatment of obesity...
  23. ncbi Precision and accuracy of an ankle-worn accelerometer-based pedometer in step counting and energy expenditure
    Randal C Foster
    Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Prev Med 41:778-83. 2005
    ..In this paper, we investigate the precision and accuracy of an ankle-worn dual-axis accelerometer (Stepwatch) and investigate its potential application as a predictor of energy expenditure...
  24. ncbi Central orexin sensitivity, physical activity, and obesity in diet-induced obese and diet-resistant rats
    Colleen M Novak
    Endocrine Research Unit, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, USA
    Am J Physiol Endocrinol Metab 290:E396-403. 2006
    ..A putative mechanism for changes in NEAT that accompany obesity is a decreased sensitivity to the NEAT-activating effects of neuropeptides such as orexin...
  25. ncbi Measurement of energy expenditure
    James A Levine
    Mayo Clinic, Endocrine Research Unit, Rochester, MN 55902, USA
    Public Health Nutr 8:1123-32. 2005
    ..The factorial method for combining activity logs and data on the energy costs of activities can also provide detailed information on free-living subjects...
  26. ncbi Non-exercise activity thermogenesis: the crouching tiger hidden dragon of societal weight gain
    James A Levine
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Arterioscler Thromb Vasc Biol 26:729-36. 2006
    ..To reverse obesity, we need to develop individual strategies to promote standing and ambulating time by 2.5 hours per day and also re-engineer our work, school, and home environments to render active living the option of choice...
  27. ncbi Weekly changes in basal metabolic rate with eight weeks of overfeeding
    Ann M Harris
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Obesity (Silver Spring) 14:690-5. 2006
    ..Our hypothesis was that BMR does not increase linearly with 1000-kcal/d overfeeding in lean healthy subjects over 8 weeks. The null hypothesis states that BMR increases linearly with 1000-kcal/d overfeeding in lean healthy subjects...
  28. ncbi Orexin A mediation of time spent moving in rats: neural mechanisms
    C M Kotz
    Veterans Affairs Medical Center, Geriatric Research, Education and Clinical Care 11G, One Veterans Drive, Minneapolis, MN 55417, USA
    Neuroscience 142:29-36. 2006
    ..This potential for conferring physical activity stimulation may be useful for inducing shifts in time spent moving, which has important implications for obesity...
  29. ncbi Neuromedin U in the paraventricular and arcuate hypothalamic nuclei increases non-exercise activity thermogenesis
    C M Novak
    Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA
    J Neuroendocrinol 18:594-601. 2006
    ..In co-ordination with its suppressive effects on feeding, the NEAT-activating effects of NMU make it a potential candidate in the combat of obesity...
  30. ncbi Energy expenditure of sedentary screen time compared with active screen time for children
    Lorraine Lanningham-Foster
    Endocrine Research Unit, Mayo Clinic, 200 First St, SW, Rochester, MN 55905, USA
    Pediatrics 118:e1831-5. 2006
    ..Our hypothesis was that energy expenditure would be significantly greater when children played activity-promoting video games, compared with sedentary video games...
  31. ncbi Nonexercise movement in elderly compared with young people
    Ann M Harris
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Am J Physiol Endocrinol Metab 292:E1207-12. 2007
    ..Thus, the energetic cost of walking in the elderly may be greater than in the young. Lean, healthy elderly individuals may have a biological drive to be less active than the young...
  32. pmc The energy expenditure of using a "walk-and-work" desk for office workers with obesity
    James A Levine
    Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA
    Br J Sports Med 41:558-61. 2007
    ..Our hypothesis was that a vertical workstation that allows an obese individual to work while walking would be associated with significant and substantial increases in energy expenditure over seated work...
  33. pmc An office-place stepping device to promote workplace physical activity
    David A McAlpine
    Mayo Clinic, 200 First St SW, Rochester 55905, USA
    Br J Sports Med 41:903-7. 2007
    ..The objective was to assess the effect of using an office-place stepping device on the energy expenditure of lean and obese office workers...
  34. ncbi Exercise: a walk in the park?
    James A Levine
    Mayo Clin Proc 82:797-8. 2007
  35. ncbi Nonexercise activity thermogenesis--liberating the life-force
    J A Levine
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 5590, USA
    J Intern Med 262:273-87. 2007
    ..Data support the central hypothesis that NEAT is pivotal in the regulation of human energy expenditure and body weight regulation and that NEAT is important for understanding the cause and effective treatment for obesity...
  36. ncbi Interindividual variation in posture allocation: possible role in human obesity
    James A Levine
    Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905, USA
    Science 307:584-6. 2005
    ..If obese individuals adopted the NEAT-enhanced behaviors of their lean counterparts, they might expend an additional 350 calories (kcal) per day...