INTRACELLULAR CALCIUM IN HAIR CELLS

Summary

Principal Investigator: Robert Fettiplace
Affiliation: University of Wisconsin
Country: USA
Abstract: DESCRIPTION (provided by applicant): The long-term objectives are to understand the cellular mechanisms of transduction in auditory hair cells and delineate the factors underlying the cochlea's tonotopic organization. Experiments will focus on transducer channel adaptation and hair bundle mechanics to define their regulation by Ca2+ and roles in frequency selectivity. A prime objective is to improve the speed of the mechanical stimulator and utilize different preparations and conditions to obtain accurate measures of the kinetics of transduction and active bundle motion. Hair cell responses will be measured in the isolated cochleae of both mammals and birds and will be combined with intracellular Ca2+ imaging. Specific aims are: (1) to record mechanotransducer currents in gerbil and rat hair cells before and after the onset of hearing, documenting the changes in kinetics and other properties with cochlear location;(2) to characterize single mechanotransducer channels and investigate their variation with cochlear location and modulation by Ca2+ and cyclic AMP, to provide a baseline for candidate channel proteins;(3) to measure the mechanical properties of hair bundles and search for spontaneous and active bundle motion in mammalian hair cells. The interaction between active bundle motion and outer hair cell contractility will be used to assess the roles of the two processes in amplification and frequency tuning in the intact mammalian cochlea. (4) to record mechanotransducer currents and measure hair bundle mechanics of short (outer) hair cells in the chick auditory papilla as a likely but unproven site where active hair bundle motion is used to augment frequency selectivity. Comparison with the properties of the mammalian hair bundles will provide insight into the evolution of cochlear amplification;(5) to measure and alter the concentration of Ca2+ in hair bundles and relate it to control of mechanotransducer channel adaptation and active hair bundle motion. The contributions of Ca2+ buffering and uptake into intracellular compartments, especially the mitochondria, to limit Ca2+ transients will be studied. Since hair cells experience large Ca2+ loads, disturbance of Ca2+ homeostasis may be a leading cause of injury. Ca2+ modulation of mechanotransducer channels is probably common to all hair cells and may be the site of irreversible damage during noise exposure, poisoning with ototoxic agents or aging. Loss of hearing with aging or over-stimulation is often restricted to high frequencies and is linked to degeneration of hair cells at one end of the cochlea. The work will address the reasons for this differential sensitivity by mapping the properties of transduction with location in the mammalian cochlea. It is hypothesized that the majority of Ca2+ enters the hair cells through the MT channels and the increased vulnerability of high frequency outer hair cells reflects a greater Ca2+ influx because of larger MT currents. Hearing impairment is the most common disabling sensory defect in humans. Severe to profound hearing loss, largely attributable to injury to the sensory hair cells, affects 1 in 1,000 newborns, and 60% of people older than 70 years have a hearing deficit of at least 25 dB. It has a range of causes, including genetic, noise or drug induced, as well as being age-related but the basic mechanisms of damage and cell death in most cases are not well understood. The work will address the mechanisms by documenting the mechanical and electrical properties of auditory hair cells and their modulation by calcium ions. We hypothesize that calcium overloading leading to mitochondrial dysfunction is a major route to cell damage.
Funding Period: 1992-01-01 - 2013-12-31
more information: NIH RePORT

Top Publications

  1. pmc The role of transmembrane channel-like proteins in the operation of hair cell mechanotransducer channels
    Kyunghee X Kim
    Department of Neuroscience, University of Wisconsin Medical School, Madison, WI 53706
    J Gen Physiol 142:493-505. 2013
  2. pmc The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells
    Qingguo Chen
    Department of Otolaryngology Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Eur J Neurosci 36:2302-10. 2012
  3. pmc The resting transducer current drives spontaneous activity in prehearing mammalian cochlear inner hair cells
    Stuart L Johnson
    Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Neurosci 32:10479-83. 2012
  4. pmc Optimal electrical properties of outer hair cells ensure cochlear amplification
    Jong Hoon Nam
    Department of Mechanical Engineering, University of Rochester, Rochester, New York, United States of America
    PLoS ONE 7:e50572. 2012
  5. pmc Developmental changes in the cochlear hair cell mechanotransducer channel and their regulation by transmembrane channel-like proteins
    Kyunghee X Kim
    Department of Neuroscience, University of Wisconsin Madison, WI 53706, USA
    J Gen Physiol 141:141-8. 2013
  6. pmc Electrical tuning and transduction in short hair cells of the chicken auditory papilla
    Xiaodong Tan
    Department of Neuroscience, University of Wisconsin Medical School, Madison, Wisconsin, USA
    J Neurophysiol 109:2007-20. 2013
  7. pmc A prestin motor in chicken auditory hair cells: active force generation in a nonmammalian species
    Maryline Beurg
    Department of Neuroscience, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA
    Neuron 79:69-81. 2013
  8. pmc Prestin-driven cochlear amplification is not limited by the outer hair cell membrane time constant
    Stuart L Johnson
    Department of Biomedical Science, University of Sheffield, Sheffield S102TN, UK
    Neuron 70:1143-54. 2011
  9. pmc Force transmission in the organ of Corti micromachine
    Jong Hoon Nam
    Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin, USA
    Biophys J 98:2813-21. 2010
  10. pmc The ultrastructural distribution of prestin in outer hair cells: a post-embedding immunogold investigation of low-frequency and high-frequency regions of the rat cochlea
    Shanthini Mahendrasingam
    School of Life Sciences, Keele University, Keele, UK
    Eur J Neurosci 31:1595-605. 2010

Scientific Experts

  • Robert Fettiplace
  • Walter Marcotti
  • Lisa Grant
  • Maryline Beurg
  • Jong Hoon Nam
  • Shanthini Mahendrasingam
  • Carole M Hackney
  • Kyunghee X Kim
  • Stuart L Johnson
  • David N Furness
  • Helen J Kennedy
  • Xiaodong Tan
  • Qingguo Chen
  • Anthony J Ricci
  • Michael G Evans
  • Andrew C Crawford
  • Carole Hackney
  • Jacqueline A Tickle
  • Matthew C Holley
  • Andrew Crawford
  • Mitsuru Ohashi
  • Andrew Penn

Detail Information

Publications22

  1. pmc The role of transmembrane channel-like proteins in the operation of hair cell mechanotransducer channels
    Kyunghee X Kim
    Department of Neuroscience, University of Wisconsin Medical School, Madison, WI 53706
    J Gen Physiol 142:493-505. 2013
    ..Changes in the MT conductance and Ca(2+) permeability observed in the absence of Tmc1 mutants may stem from loss of interaction with protein partners in the transduction complex. ..
  2. pmc The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells
    Qingguo Chen
    Department of Otolaryngology Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Eur J Neurosci 36:2302-10. 2012
    ..The limited ability of PMCA2 to extrude the Ca(2+) load through MT channels may constitute a major cause of OHC vulnerability and high-frequency hearing loss...
  3. pmc The resting transducer current drives spontaneous activity in prehearing mammalian cochlear inner hair cells
    Stuart L Johnson
    Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Neurosci 32:10479-83. 2012
    ..We propose that, in vivo, spontaneous Ca(2+) action potentials are intrinsically generated by IHCs up to the onset of hearing and that they are likely to influence the final sensory-independent refinement of the developing cochlea...
  4. pmc Optimal electrical properties of outer hair cells ensure cochlear amplification
    Jong Hoon Nam
    Department of Mechanical Engineering, University of Rochester, Rochester, New York, United States of America
    PLoS ONE 7:e50572. 2012
    ..We conclude that the components of OHC forward and reverse transduction are crucial for setting the phase relations needed for amplification...
  5. pmc Developmental changes in the cochlear hair cell mechanotransducer channel and their regulation by transmembrane channel-like proteins
    Kyunghee X Kim
    Department of Neuroscience, University of Wisconsin Madison, WI 53706, USA
    J Gen Physiol 141:141-8. 2013
    ..Changes in P(Ca) with maturation are consistent with a developmental decrease in abundance of Tmc2 in OHCs but not in IHCs...
  6. pmc Electrical tuning and transduction in short hair cells of the chicken auditory papilla
    Xiaodong Tan
    Department of Neuroscience, University of Wisconsin Medical School, Madison, Wisconsin, USA
    J Neurophysiol 109:2007-20. 2013
    ..Stereociliary bundles also labeled heavily with antibodies against the Ca(2+) pump isoform PMCA2a...
  7. pmc A prestin motor in chicken auditory hair cells: active force generation in a nonmammalian species
    Maryline Beurg
    Department of Neuroscience, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA
    Neuron 79:69-81. 2013
    ..Our results imply that the first use of prestin as a motor protein occurred early in amniote evolution and was not a mammalian invention as is usually supposed. ..
  8. pmc Prestin-driven cochlear amplification is not limited by the outer hair cell membrane time constant
    Stuart L Johnson
    Department of Biomedical Science, University of Sheffield, Sheffield S102TN, UK
    Neuron 70:1143-54. 2011
    ..These data suggest that minimal τ(m) filtering in vivo ensures optimal activation of prestin...
  9. pmc Force transmission in the organ of Corti micromachine
    Jong Hoon Nam
    Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin, USA
    Biophys J 98:2813-21. 2010
    ..Compared to the hair bundle motor, the somatic motor was more effective in deforming the organ of Corti than in displacing the basilar membrane...
  10. pmc The ultrastructural distribution of prestin in outer hair cells: a post-embedding immunogold investigation of low-frequency and high-frequency regions of the rat cochlea
    Shanthini Mahendrasingam
    School of Life Sciences, Keele University, Keele, UK
    Eur J Neurosci 31:1595-605. 2010
    ..The results suggest that prestin density, and by implication force production, is similar in low-frequency and high-frequency OHCs...
  11. pmc Calcium balance and mechanotransduction in rat cochlear hair cells
    Maryline Beurg
    Institut National de la Santé et de la Recherche Médicale U587, Universite Victor Segalen Bordeaux 2, Hopital Pellegrin, Bordeaux, France
    J Neurophysiol 104:18-34. 2010
    ..The model also indicated the endogenous mobile buffer was equivalent to 1 mM BAPTA...
  12. pmc Defining features of the hair cell mechanoelectrical transducer channel
    Robert Fettiplace
    Department of Physiology, University of Wisconsin Medical School, 185 Medical Sciences Building, 1300 University Avenue, Madison, WI 53706, USA
    Pflugers Arch 458:1115-23. 2009
    ..The strengths and weaknesses of a TRP channel contender are discussed...
  13. ncbi The concentrations of calcium buffering proteins in mammalian cochlear hair cells
    Carole M Hackney
    MacKay Institute of Communication and Neuroscience, School of Life Sciences, Keele University, Keele, Staffordshire ST5 5BG, United Kingdom
    J Neurosci 25:7867-75. 2005
    ..The high concentration of calcium buffer in outer hair cells, similar only to skeletal muscle, may protect against deleterious consequences of Ca2+ loading after acoustic overstimulation...
  14. ncbi Depolarization of cochlear outer hair cells evokes active hair bundle motion by two mechanisms
    Helen J Kennedy
    Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Neurosci 26:2757-66. 2006
    ..It is conceivable that the two processes can interact, a signal from the MET channels being capable of modulating the activity of the prestin motor...
  15. pmc Active hair bundle movements in auditory hair cells
    Robert Fettiplace
    185 Medical Sciences Building, 1300, University Avenue, Madison, WI 53706, USA
    J Physiol 576:29-36. 2006
    ..This review summarizes evidence for active hair bundle motion and its connection to MT channel adaptation. Key factors for the hair bundle motor to play a role in the mammalian cochlea include the size and speed of force production...
  16. ncbi Ryanodine receptor localisation in the mammalian cochlea: an ultrastructural study
    Lisa Grant
    Department of Physiology, University of Bristol, Bristol, UK
    Hear Res 219:101-9. 2006
    ..CICR may be involved in the control of calcium levels in the base of the inner hair cells and supporting cells, and in the cholinergic efferent response and motile behaviour of the outer hair cells...
  17. ncbi A large-conductance calcium-selective mechanotransducer channel in mammalian cochlear hair cells
    Maryline Beurg
    Equipe Associée 3665 Université Victor Segalen Bordeaux 2, Institut National de la Sante et de la Recherche Medicale, Unité 587, Hopital Pellegrin, 33076 Bordeaux, France
    J Neurosci 26:10992-1000. 2006
    ..The results imply that the MT channel has properties possessed by few known candidates, and its diversity suggests expression of multiple isoforms...
  18. pmc The actions of calcium on hair bundle mechanics in mammalian cochlear hair cells
    Maryline Beurg
    INSERM U587, Universite Victor Segalen Bordeaux, Hopital Pellegrin, Bordeaux, France
    Biophys J 94:2639-53. 2008
    ..The significance of these results for cochlear transduction and amplification are discussed...
  19. pmc The dimensions and composition of stereociliary rootlets in mammalian cochlear hair cells: comparison between high- and low-frequency cells and evidence for a connection to the lateral membrane
    David N Furness
    Institute of Science and Technology in Medicine, Keele University, Keele, Staffordshire ST5 5BG, United Kingdom
    J Neurosci 28:6342-53. 2008
    ....
  20. pmc Theoretical conditions for high-frequency hair bundle oscillations in auditory hair cells
    Jong Hoon Nam
    Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin, USA
    Biophys J 95:4948-62. 2008
    ..Spontaneous oscillations persisted, becoming very narrow-band, when the hair bundle was loaded with a tectorial membrane mass...
  21. pmc Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging
    Maryline Beurg
    INSERM U, Universite Victor Segalen Bordeaux, Hopital Pellegrin, France
    Nat Neurosci 12:553-8. 2009
    ..The observations, supported by theoretical simulations, suggest there are no functional mechanically sensitive transducer channels in first row stereocilia and imply the channels are present only at the bottom of the tip links...
  22. ncbi The transduction channel filter in auditory hair cells
    Anthony J Ricci
    Center for Neuroscience, Louisiana State University Medical Center, New Orleans, Louisiana 70112, USA
    J Neurosci 25:7831-9. 2005
    ....