Repair of Oxidatively Damaged Guanines

Summary

Principal Investigator: A Lien Lu
Affiliation: University of Maryland
Country: USA
Abstract: Oxidative DNA damage presents a serious challenge to genomic integrity and can accelerate carcinogenesis and aging. Reactive oxygen species-dependent DNA damage are repaired by base excision repair (BER), initiated with removal of base lesions by DNA glycosylases. The overall goal of this project is to study the role of selected DNA glycosylases and mismatch repair enzymes in response to cellular oxidative stress. We focus on the role of human MutY homolog (hMYH) glycosylase and its interactions with cell cycle and aging regulators. hMYH reduces stress-induced mutagenesis by removing misincorporated adenines paired with 8-oxoG (the most abundant form of DNA damage), therefore reduces G:C to T:A mutations. hMYH deficiency predispose individuals to colon cancer. hMYH interacts with the DNA replication machinery, other repair enzymes, the 9-1-1 cell cycle checkpoint complex (Rad9/Rad1/Hus1), and the aging regulator SIRT6. In this context, the 9-1-1 proteins interact with and increase the activities DNA glycosylases and hMSH2/hMSH6 mismatch recognition complex. We hypothesize that MYH and other DNA repair enzymes serve as molecular adaptors to recruit checkpoint proteins to DNA lesion sites and coordinate DNA repair and increase repair efficiency and fidelity. To examine this hypothesis, we propose three specific aims: (1) The dynamic interaction of MYH with MSH2/MSH6 both in vitro and in vivo will be delineated. We will test whether these interactions are altered following oxidative stress and during the progression of the cell cycle. (2) The physical and functional Interactions of MYH (and Neil1, hOGG1 and hMSH2/hMSH6) with the 9-1-1 complex will be elucidated. We will investigate how different proteins compete for the 9-1-1 complex and why different glycosylases select different subunits of the 9-1-1 complex. The biological significance of Hus1-MYH interaction will be investigated by interruption of the interaction by mutagenesis and by using a Hus1 binding competitor peptide. We will test a model that DNA glycosylases act as adaptors to recruit the 9-1-1 complex to the lesion sites. (3) The novel role of an aging regulator SIRT6 in DNA repair, cell cycle control, and aging will be studied. SIRT6 interacts with the 9-1-1 complex and has a role in BER by stimulating MYH, but inhibiting NEIL1 activity. We will test whether expression of DNA glycosylase can influence the sensitivity of Sirt6 deficient cells to DNA damage agents and whether SIRT6 can deacetylate hNEIL1. Because SIRT6 is required to regulate genomic integrity and impacts the aging process, revealing the mechanism of SIRT6 interaction in BER and cell cycle checkpoints is important. Successful completion of these studies will reveal important new information regarding the interactions among DNA repair proteins, cell cycle checkpoints, and an aging regulating protein. These studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. PUBLIC HEALTH RELEVANCE: The overall goal of this project is to address the roles of several DNA glycosylases and mismatch repair enzymes in cancer prevention. Defects in DNA repair, cell cycle checkpoint activation, and gene silencer SIRT6 are associated with cancer and aging. The findings from our studies will have implications for treating human disease and cancer.
Funding Period: ----------------1998 - ---------------2014-
more information: NIH RePORT

Top Publications

  1. pmc Functional characterization of human MutY homolog (hMYH) missense mutation (R231L) that is linked with hMYH-associated polyposis
    Haibo Bai
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
    Cancer Lett 250:74-81. 2007
  2. pmc Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1
    Haibo Bai
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
    DNA Repair (Amst) 9:478-87. 2010
  3. pmc A structural hinge in eukaryotic MutY homologues mediates catalytic activity and Rad9-Rad1-Hus1 checkpoint complex interactions
    Paz J Luncsford
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA
    J Mol Biol 403:351-70. 2010
  4. doi Histone deacetylase SIRT1 modulates and deacetylates DNA base excision repair enzyme thymine DNA glycosylase
    Amrita Madabushi
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, U S A
    Biochem J 456:89-98. 2013
  5. pmc Coordination of MYH DNA glycosylase and APE1 endonuclease activities via physical interactions
    Paz J Luncsford
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
    DNA Repair (Amst) 12:1043-52. 2013
  6. ncbi Mammalian MutY homolog (MYH or MUTYH) protects cells from oxidative DNA damage
    Bor Jang Hwang
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
    DNA Repair (Amst) 13:10-21. 2014
  7. pmc Interaction of apurinic/apyrimidinic endonuclease 2 (Apn2) with Myh1 DNA glycosylase in fission yeast
    Jin Jin
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    DNA Repair (Amst) 15:1-10. 2014
  8. pmc The role of MutY homolog (Myh1) in controlling the histone deacetylase Hst4 in the fission yeast Schizosaccharomyces pombe
    Dau Yin Chang
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 108 North Greene Street, Baltimore, MD 21201, USA
    J Mol Biol 405:653-65. 2011

Detail Information

Publications8

  1. pmc Functional characterization of human MutY homolog (hMYH) missense mutation (R231L) that is linked with hMYH-associated polyposis
    Haibo Bai
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
    Cancer Lett 250:74-81. 2007
    ..The mutant fails to complement mutY-deficiency in Escherichia coli, but does not affect binding to hMSH6. These data support the role of the hMYH pathway in carcinogenesis...
  2. pmc Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1
    Haibo Bai
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
    DNA Repair (Amst) 9:478-87. 2010
    ..Our findings suggest that the 9-1-1 complex is a component of the mismatch repair involved in MNNG-induced damage response...
  3. pmc A structural hinge in eukaryotic MutY homologues mediates catalytic activity and Rad9-Rad1-Hus1 checkpoint complex interactions
    Paz J Luncsford
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA
    J Mol Biol 403:351-70. 2010
    ..Thus, preserving the MYH/9-1-1 interaction contributes significantly to minimizing the mutagenic potential of oxidative DNA damage...
  4. doi Histone deacetylase SIRT1 modulates and deacetylates DNA base excision repair enzyme thymine DNA glycosylase
    Amrita Madabushi
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, U S A
    Biochem J 456:89-98. 2013
    ..The physical and functional interactions between SIRT1 and TDG may mediate DNA repair, gene expression and FU (5-fluorouracil)-mediated cytotoxicity. ..
  5. pmc Coordination of MYH DNA glycosylase and APE1 endonuclease activities via physical interactions
    Paz J Luncsford
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
    DNA Repair (Amst) 12:1043-52. 2013
    ....
  6. ncbi Mammalian MutY homolog (MYH or MUTYH) protects cells from oxidative DNA damage
    Bor Jang Hwang
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
    DNA Repair (Amst) 13:10-21. 2014
    ..In conclusion, MYH is a vital DNA repair enzyme that protects cells from oxidative DNA damage and is critical for a proper cellular response to DNA damage. ..
  7. pmc Interaction of apurinic/apyrimidinic endonuclease 2 (Apn2) with Myh1 DNA glycosylase in fission yeast
    Jin Jin
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    DNA Repair (Amst) 15:1-10. 2014
    ..The cross stimulation of Myh1 and Apn2 enzymatic activities is dependent on their physical interaction. Thus, Myh1 and Apn2 constitute an initial BER complex. ..
  8. pmc The role of MutY homolog (Myh1) in controlling the histone deacetylase Hst4 in the fission yeast Schizosaccharomyces pombe
    Dau Yin Chang
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 108 North Greene Street, Baltimore, MD 21201, USA
    J Mol Biol 405:653-65. 2011
    ..These results suggest that SpMyh1 repair regulates the functions of SpHst4 and the 9-1-1 complex in maintaining genomic stability...