Intestinal Microbiota, Diet and Risk of Colorectal Adenomas

Summary

Principal Investigator: Temitope O Keku
Affiliation: University of North Carolina
Country: USA
Abstract: DESCRIPTION (provided by applicant): Evidence from animal and human studies suggests that intestinal bacteria may contribute to the pathogenesis of colorectal cancer (CRC), a major leading cause of cancer mortality in the United States. Potential mechanisms for the link between gut microbiota and CRC is through diet and inflammation. We propose a model whereby intestinal bacteria play a prominent role in the etiology of CRC through diet, inflammation and metabolism of xenobiotics. We propose to test the hypothesis that adherent bacteria (adherent) are linked with elevated risk of colorectal adenoma and that these bacteria modulate the association between diet, inflammation and colorectal adenomas. We propose that distinct patterns of commensal colonization or presence/absence of specific bacteria species will correlate with adenoma risk. The specific aims are to 1) determine whether the adherent (mucosa-associated) bacteria community composition and structure (profiles) differ between subjects with adenomas and those without adenomas, 2) evaluate the associations of adherent bacteria profiles and systemic or local markers of inflammation (IL-12, IL-23, IL-4, IL-17, INF(, IL-8, IL-10 and TGF-(;macrophages, NK cells, T cells- CD4+ and CD8+;protein expression of NF-(B (p65) and STAT3) among subjects with and without adenomas, 3) assess the association between adherent bacteria profiles and diet/lifestyle such as fiber, meat intake, obesity (body mass index (BMI), waist-hip-ratio), and NSAID use in relation to colorectal adenomas. Evaluation of the diversity of gut bacteria in relation to disease in humans is limited in part, by the difficulty growing these organisms in culture. Recent advances in molecular methods have made it possible to assess the role of intestinal microbiota in diseases such as colon cancer. To test our hypothesis, we propose to use molecular-phylogenetic methods based on the highly conserved 16S bacteria rRNA gene to assess the contribution of intestinal microbiota to the development of colorectal adenomas. These methods include PCR amplification of the 16S rRNA gene, terminal restriction fragment length polymorphism (TRFLP), generation of bacteria clone libraries and sequencing. This study will use colonic biopsy specimens obtained from 600 patients (300 cases and 300 controls) and risk factor data such as diet and inflammation from a funded ongoing study of colorectal adenomas, the Diet and Health Study (NCI R01 CA 44684). Limited information exists on the role of gut bacteria in the development of adenomas. This study will provide critical insights on the composition and diversity of the microbiota and their association with colorectal adenomas and known risk factors. The findings from this study could lead to the development of strategies to manipulate the intestinal microbiota to prevent colorectal adenomas and cancer as well as identify individuals at high risk. PUBLIC HEALTH RELEVANCE: The goal of this study is to evaluate the composition and structure of bacteria communities that reside on the lining of the large bowel, in relation to colorectal adenomas and known risk factors such as diet and inflammation. The findings from this study could help identify individuals at high risk of adenomas as well as enhance the development of strategies to manipulate the intestinal microbiota to prevent colorectal adenomas and cancer
Funding Period: 2009-05-01 - 2014-02-28
more information: NIH RePORT

Top Publications

  1. ncbi Gut microbiome and colorectal adenomas
    Santosh Dulal
    From the Center for Gastrointestinal Biology and Disease, and Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, and School of Medicine, University of North Carolina at Chapel Hill, NC
    Cancer J 20:225-31. 2014
  2. pmc Altered tissue metabolites correlate with microbial dysbiosis in colorectal adenomas
    Julia L Nugent
    School of Medicine, University of North Carolina at Chapel Hill, 321 South Columbia Street, Chapel Hill, North Carolina 27599, United States
    J Proteome Res 13:1921-9. 2014
  3. pmc Fusobacterium spp. and colorectal cancer: cause or consequence?
    Temitope O Keku
    Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA Electronic address
    Trends Microbiol 21:506-8. 2013
  4. pmc Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
    Melissa Kang
    Center for Gastrointestinal Biology and Disease, University of North Carolina, 103 Mason Farm Road, 7340 Medical Biomolecular Research Building, CB 7032, 27599 7032, Chapel Hill, NC 27599, USA
    BMC Cancer 13:91. 2013
  5. pmc Fusobacterium is associated with colorectal adenomas
    Amber N McCoy
    Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    PLoS ONE 8:e53653. 2013
  6. pmc Differences in microbial signatures between rectal mucosal biopsies and rectal swabs
    Felix Araujo-Perez
    Division of Gastroenterology and Hepatology, Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Gut Microbes 3:530-5. 2012
  7. pmc Intestinal inflammation targets cancer-inducing activity of the microbiota
    Janelle C Arthur
    Department of Medicine, Pharmacology and Immunology Microbiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 338:120-3. 2012
  8. pmc Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans
    Nina Sanapareddy
    Department of Bioinformatics and Genomics, University of North Carolina, Charlotte, NC, USA
    ISME J 6:1858-68. 2012
  9. pmc Molecular characterization of mucosal adherent bacteria and associations with colorectal adenomas
    Xiang Jun Shen
    Center for Gastrointestinal Biology and Disease University of North Carolina at Chapel Hill, NC, USA
    Gut Microbes 1:138-47. 2010

Detail Information

Publications10

  1. ncbi Gut microbiome and colorectal adenomas
    Santosh Dulal
    From the Center for Gastrointestinal Biology and Disease, and Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, and School of Medicine, University of North Carolina at Chapel Hill, NC
    Cancer J 20:225-31. 2014
    ..Understanding the microbiome may provide promising new directions towards novel diagnostic tools, biomarkers, and therapeutic interventions for CRC. ..
  2. pmc Altered tissue metabolites correlate with microbial dysbiosis in colorectal adenomas
    Julia L Nugent
    School of Medicine, University of North Carolina at Chapel Hill, 321 South Columbia Street, Chapel Hill, North Carolina 27599, United States
    J Proteome Res 13:1921-9. 2014
    ..These findings suggest that metabolic products of bacteria may be responsible for the development of colorectal adenomas and CRC. ..
  3. pmc Fusobacterium spp. and colorectal cancer: cause or consequence?
    Temitope O Keku
    Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA Electronic address
    Trends Microbiol 21:506-8. 2013
    ..Recent studies have implicated overabundance of Fusobacterium in association with colorectal adenomas and cancer. Two articles published in Cell Host & Microbe provide insights into the Fusobacterium-CRC relationship. ..
  4. pmc Association of plasma endotoxin, inflammatory cytokines and risk of colorectal adenomas
    Melissa Kang
    Center for Gastrointestinal Biology and Disease, University of North Carolina, 103 Mason Farm Road, 7340 Medical Biomolecular Research Building, CB 7032, 27599 7032, Chapel Hill, NC 27599, USA
    BMC Cancer 13:91. 2013
    ..Given the evidence linking inflammation and colorectal cancer, we sought to determine if plasma endotoxin concentrations are associated with indicators of systemic or local inflammation and colorectal adenomas...
  5. pmc Fusobacterium is associated with colorectal adenomas
    Amber N McCoy
    Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    PLoS ONE 8:e53653. 2013
    ..33, p = 0.06 while it was 0.44, p = 0.01 for Fusobacterium and IL-10. These results support a link between the abundance of Fusobacterium in colonic mucosa and adenomas and suggest a possible role for mucosal inflammation in this process...
  6. pmc Differences in microbial signatures between rectal mucosal biopsies and rectal swabs
    Felix Araujo-Perez
    Division of Gastroenterology and Hepatology, Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Gut Microbes 3:530-5. 2012
    ..0001) and Eubacteria (p = 0.0003) in swab samples compared with biopsies. Our findings suggest that rectal swabs and rectal mucosal samples provide different views of the microbiota in the large intestine...
  7. pmc Intestinal inflammation targets cancer-inducing activity of the microbiota
    Janelle C Arthur
    Department of Medicine, Pharmacology and Immunology Microbiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 338:120-3. 2012
    ..This suggests that in mice, colitis can promote tumorigenesis by altering microbial composition and inducing the expansion of microorganisms with genotoxic capabilities...
  8. pmc Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans
    Nina Sanapareddy
    Department of Bioinformatics and Genomics, University of North Carolina, Charlotte, NC, USA
    ISME J 6:1858-68. 2012
    ..This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas...
  9. pmc Molecular characterization of mucosal adherent bacteria and associations with colorectal adenomas
    Xiang Jun Shen
    Center for Gastrointestinal Biology and Disease University of North Carolina at Chapel Hill, NC, USA
    Gut Microbes 1:138-47. 2010
    ..Extension of these findings could lead to strategies to manipulate the microbiota to prevent colorectal adenomas and cancer as well as to identify individuals at high risk...