Biofilm Development by Vibrio cholerae

Summary

Principal Investigator: Paula Watnick
Affiliation: Harvard University
Country: USA
Abstract: Cholera is a severe epidemic diarrheal disease caused by the gram-negative aquatic bacterium Vibrio cholerae. As is true for most bacterial pathogens, attachment to biotic surfaces such as the exoskeletae of copepods and the intestinal epithelium is critical to survival of V. cholerae. V. cholerae can either attach to surfaces as a monolayer, which is defined by the presence of cell-surface adhesion without interbacterial adhesion, or as a biofilm, which is defined by the presence of cell-surface and interbacterial adhesions. In the previous funding period of this grant, we focused on defining the environmental signals, genetic requirements, and gene transcription profiles that defined the V. cholerae monolayer and biofilm. During the next funding period, we propose to investigate three observations made during the previous funding period. These are (i) monosaccharides are strong inducers of biofilm development and transcription of genes encoding monosaccharide transporters is co-regulated with biofilm exopolysaccharide synthesis gene transcription, (ii) secreted proteins play a role in biofilm matrix formation, and (iii) transcription of a putative undecaprenyl pyrophosphate phosphatase is regulated similarly to that of biofilm exopolysaccharide synthesis genes and is required for biofilm formation. The relevant specific aims for this funding period are: 1) To investigate regulation of surface-associated growth in glucose-rich environments and to define its relevance to survival within the host gastrointestinal tract. 2) To characterize the functions of proteinaceous components of the Vibrio cholerae biofilm matrix. 3) To study the role of an undecaprenyl pyrophosphate phosphatase in V. cholerae biofilm matrix synthesis.
Funding Period: ----------------2002 - ---------------2011-
more information: NIH RePORT

Top Publications

  1. pmc A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 190:311-20. 2008
  2. pmc Genetic analysis of Vibrio cholerae monolayer formation reveals a key role for DeltaPsi in the transition to permanent attachment
    Katrina L Van Dellen
    Division of Infectious Disease, Children s Hospital Boston, Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Bacteriol 190:8185-96. 2008
  3. pmc Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Infect Immun 78:1482-94. 2010
  4. pmc The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 192:3055-67. 2010
  5. pmc Signals, regulatory networks, and materials that build and break bacterial biofilms
    Ece Karatan
    Department of Biology, Appalachian State University, Boone, NC 28608, USA
    Microbiol Mol Biol Rev 73:310-47. 2009
  6. pmc A communal bacterial adhesin anchors biofilm and bystander cells to surfaces
    Cedric Absalon
    Division of Infectious Disease, Children s Hospital, Boston, Massachusetts, United States of America
    PLoS Pathog 7:e1002210. 2011

Scientific Experts

  • Paula Watnick
  • Ece Karatan
  • Laetitia Houot
  • Cedric Absalon
  • Sarah Chang
  • Katrina L Van Dellen
  • Katrina Van Dellen
  • Bradley S Pickering

Detail Information

Publications6

  1. pmc A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 190:311-20. 2008
    ..As the PTS is highly conserved among bacteria, the enzyme I regulatory pathway may be relevant to a number of biofilm-based infections...
  2. pmc Genetic analysis of Vibrio cholerae monolayer formation reveals a key role for DeltaPsi in the transition to permanent attachment
    Katrina L Van Dellen
    Division of Infectious Disease, Children s Hospital Boston, Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Bacteriol 190:8185-96. 2008
    ..We propose that as a bacterium approaches a surface, the interaction of the flagellum with the surface leads to transient hyperpolarization of the bacterial cell membrane. This, in turn, initiates the transition to permanent attachment...
  3. pmc Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Infect Immun 78:1482-94. 2010
    ..cholerae through its life cycle of pathogenesis and environmental persistence...
  4. pmc The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways
    Laetitia Houot
    Division of Infectious Disease, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Bacteriol 192:3055-67. 2010
    ..The presence of multiple, independent biofilm regulatory circuits in the PTS supports the hypothesis that the PTS and PTS-dependent substrates have a central role in sensing environments suitable for a surface-associated existence...
  5. pmc Signals, regulatory networks, and materials that build and break bacterial biofilms
    Ece Karatan
    Department of Biology, Appalachian State University, Boone, NC 28608, USA
    Microbiol Mol Biol Rev 73:310-47. 2009
    ....
  6. pmc A communal bacterial adhesin anchors biofilm and bystander cells to surfaces
    Cedric Absalon
    Division of Infectious Disease, Children s Hospital, Boston, Massachusetts, United States of America
    PLoS Pathog 7:e1002210. 2011
    ..These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm...