RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)

Summary

Principal Investigator: DAVID JONATHAN BRENNER
Abstract: The overall technological goal is to upgrade our unique single-particle microbeam facility to improve the spatial resolution from +/- 3.5 mum, and to allow the use of more densely-ionizing radiations (i.e. heavier ion beams, with stopping powers from 15 to 4,500 keV/mum), compared with our current beams of H and He ins (stopping power range 15 to 200 keV/mum). The main specific technological goals are: 1. replacing the current physical collimator system with electrostatic lenses, that we will design and build. 2. replacing the current multiple-magnet beam transport system with a single magnet; 3. designing and building an on-line beam-shape verification system; 4. improving our cellular/sub-cellular optical identification system to a level commensurate with the +/- 0.3 mum resolution of the microbeam; 5. replacing the current ion source with a laser ion source that we shall design and build, allowing high charge-state ion bemas of essentially any element to be accelerated; 6. design/optimization/fabrication of the ion transport system to function independently of the accelerated ion type; 7. design/fabrication of a new differentially-pumped gaseous ion detector, within the beam optics system. In collaboration with investigators from outside and within Columbia University, 28 collaborative- and service-outgrowths of funded NIH research, many at the current RARAF facility. 7 of these projects originate from Columbia University, and 21 from other institutions. Training and information dissemination will continue to be important components of this resource. Training will continue to play an important role at the undergraduate, graduate, post doctoral, and senior scientist levels. Information dissemination will continue through organizing bi- annual international microbeam workshops, talks at national and international meetings, written and web-based user information, and peer- reviewed publications.
Funding Period: 1996-09-30 - 2004-08-31
more information: NIH RePORT

Top Publications

  1. pmc Radiation-induced non-targeted response in vivo: role of the TGFβ-TGFBR1-COX-2 signalling pathway
    Y Chai
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA
    Br J Cancer 108:1106-12. 2013
  2. pmc Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice
    Y Chai
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA
    Br J Cancer 108:91-8. 2013
  3. pmc Mitochondrial function and nuclear factor-kappaB-mediated signaling in radiation-induced bystander effects
    Hongning Zhou
    Center for Radiological Research, College of Physicians and Surgeons, Mailman School of Public Health, Columbia University, New York, NY, USA
    Cancer Res 68:2233-40. 2008
  4. pmc Mechanism of radiation carcinogenesis: role of the TGFBI gene and the inflammatory signaling cascade
    Tom K Hei
    Department of Radiation Oncology, Columbia University Medical Center, New York, NY 10032, USA
    Adv Exp Med Biol 720:163-70. 2011
  5. pmc Intrachromosomal changes and genomic instability in site-specific microbeam-irradiated and bystander human-hamster hybrid cells
    Burong Hu
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Radiat Res 177:25-34. 2012
  6. pmc The linear-quadratic model is an appropriate methodology for determining isoeffective doses at large doses per fraction
    David J Brenner
    Center for Radiological Research, Columbia University Medical Center, 630 West 168th Street, New York, NY 10032, USA
    Semin Radiat Oncol 18:234-9. 2008
  7. ncbi Mechanism of radiation-induced bystander effects: a unifying model
    Tom K Hei
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    J Pharm Pharmacol 60:943-50. 2008
  8. ncbi Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses
    Brian Ponnaiya
    Center for Radiological Research, Columbia University, New York, NY 10533, USA
    Exp Hematol 35:64-8. 2007
  9. ncbi Cyclooxygenase-2 as a signaling molecule in radiation-induced bystander effect
    Tom K Hei
    Center for Radiological Research, College of Physicians and Surgeons, and Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York 10032, USA
    Mol Carcinog 45:455-60. 2006
  10. pmc Mechanism of radiation-induced bystander effect: role of the cyclooxygenase-2 signaling pathway
    Hongning Zhou
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 102:14641-6. 2005

Scientific Experts

  • Brian Ponnaiya
  • TOM HEI
  • DAVID JONATHAN BRENNER
  • Y Chai
  • Hongning Zhou
  • H Zhou
  • G M Calaf
  • Burong Hu
  • Charles R Geard
  • Vladimir N Ivanov
  • S A Ghandhi
  • S A Amundson
  • R K K Lam
  • S Amundson
  • T Nohmi
  • C D Elliston
  • G Wen
  • Jing Nie
  • Peter Grabham
  • Adayabalam S Balajee
  • Mercy Davidson
  • Yu Chin Lien
  • Zengliang Yu
  • Joseph Gillespie
  • Sally A Amundson
  • Howard B Lieberman

Detail Information

Publications11

  1. pmc Radiation-induced non-targeted response in vivo: role of the TGFβ-TGFBR1-COX-2 signalling pathway
    Y Chai
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA
    Br J Cancer 108:1106-12. 2013
    ..However, the signalling pathways involved in such effects remain unclear...
  2. pmc Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice
    Y Chai
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA
    Br J Cancer 108:91-8. 2013
    ..Although radiation-induced bystander effects have been confirmed using a variety of endpoints, the mechanism(s) underlying these effects are not well understood, especially for in vivo study...
  3. pmc Mitochondrial function and nuclear factor-kappaB-mediated signaling in radiation-induced bystander effects
    Hongning Zhou
    Center for Radiological Research, College of Physicians and Surgeons, Mailman School of Public Health, Columbia University, New York, NY, USA
    Cancer Res 68:2233-40. 2008
    ....
  4. pmc Mechanism of radiation carcinogenesis: role of the TGFBI gene and the inflammatory signaling cascade
    Tom K Hei
    Department of Radiation Oncology, Columbia University Medical Center, New York, NY 10032, USA
    Adv Exp Med Biol 720:163-70. 2011
    ..The involvement of NFκB-dependent cytokines and the resultant inflammatory response works in concert with in modulating radiation-induced bronchial carcinogenesis...
  5. pmc Intrachromosomal changes and genomic instability in site-specific microbeam-irradiated and bystander human-hamster hybrid cells
    Burong Hu
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Radiat Res 177:25-34. 2012
    ..These results suggest that genomic instability that is manifested after ionizing radiation exposure is not dependent on direct damage to the cell nucleus...
  6. pmc The linear-quadratic model is an appropriate methodology for determining isoeffective doses at large doses per fraction
    David J Brenner
    Center for Radiological Research, Columbia University Medical Center, 630 West 168th Street, New York, NY 10032, USA
    Semin Radiat Oncol 18:234-9. 2008
    ..To date, there is no evidence of problems when the LQ model has been applied in the clinic...
  7. ncbi Mechanism of radiation-induced bystander effects: a unifying model
    Tom K Hei
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    J Pharm Pharmacol 60:943-50. 2008
    ....
  8. ncbi Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses
    Brian Ponnaiya
    Center for Radiological Research, Columbia University, New York, NY 10533, USA
    Exp Hematol 35:64-8. 2007
    ..Therefore, using the microbeam it is possible to examine individual cell responses in both hit and nonhit cells in the same population...
  9. ncbi Cyclooxygenase-2 as a signaling molecule in radiation-induced bystander effect
    Tom K Hei
    Center for Radiological Research, College of Physicians and Surgeons, and Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York 10032, USA
    Mol Carcinog 45:455-60. 2006
    ....
  10. pmc Mechanism of radiation-induced bystander effect: role of the cyclooxygenase-2 signaling pathway
    Hongning Zhou
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 102:14641-6. 2005
    ..These results provide evidence that the COX-2-related pathway, which is essential in mediating cellular inflammatory response, is the critical signaling link for the bystander phenomenon...