WFUSM Macaca fascicularis SPF Breeding Colony
Principal Investigator: J D Wagner
Affiliation: Wake Forest University School of Medicine
Abstract: It is generally recognized that nonhuman primates comprise an invaluable resource for biomedical research. The cynomolgus monkey (Macaca fascicularis) is one of the two most widely used macaque species, with biomedical applications in infectious disease and immune disorders, diabetes and cardiovascular disease, women's health, osteoporosis, and reproductive tract cancer. We proposed to expand an existing demonstration colony of specific pathogen free (SPF) cynomolgus monkeys to function as a national resource for NIH grantees. This proposal involves a collaboration between Wake Forest University School of Medicine (WFUSM) and the Washington National Primate Research Center (WaNPRC). WFUSM will provide infrastructure, colony management, genetic monitoring, and expertise in chronic disease modeling (cardiovascular, skeletal, metabolic, and oncologic disorders), while the WaNPRC contributes expertise in virology, immunology, and SPF breeding. Establishment of a domestic breeding colony of defined pathogen status will provide many advantages, including direct access to source animals and insulation from potential interruptions of supply due to political unrest, terrorism, shipping delays, and international quarantine. The colony will be located in a region at low risk of natural disasters (flood, hurricane, tornado, and earthquake).The breeding colony will produce animals negative for Mycobacterium tuberculosis (TB), Simian Retrovirus(SRV), Simian T-Lymphotrophic Virus (STLV), Simian Immunodeficiency Virus (SIV), and Cercopithecine Herpes Virus 1 (CHV-1). This SPF status will allow a broader range of research use for progeny animals, and provides a significant benefit to research staff by removal of latent zoonotic transmission of CHV-1, a potentially lethal pathogen. In addition to their SPF status, the monkeys will be fed a custom-prepared diet free of pesticides and other endocrine disrupters. Most commercial monkey chows contain isoflavones; these bind to estrogen receptors and have numerous biological actions. As part of the colony research program to understand better how diet many influence fetal programming and later chronic disease risk, half of the monkeys will be fed an isoflavone-free diet and half fed a diet with isoflavones at levels similar to those found in commercial chow. These conditions will provide a unique resource of both SPF and diet-defined Macaca fascicularis available for NIH-sponsored biomedical research.
Funding Period: 2007-07-01 - 2012-04-30
more information: NIH RePORT
- Resource brief: the National Non-Human Primate DNA BankBetsy Ferguson
Oregon National Primate Research Center, Oregon Health and Sciences University, 505 NW 185th Ave, Beaverton, OR 97006, USA
Methods 49:3-4. 2009..The National NHP DNA Bank includes specified holdings within each of the eight National Primate Research Centers, though detailed information on the entire collection is available through a common website...
- Life stage differences in mammary gland gene expression profile in non-human primatesPetra Stute
Department of Gynecologic Endocrinology and Reproductive Medicine, University Women s Hospital, Berne, Switzerland
Breast Cancer Res Treat 133:617-34. 2012..Several of the pathways activated during pubertal development have been implicated in cancer development and metastasis, supporting the idea that other developmental markers may have application as biomarkers for BC...
- Nonhuman primates and other animal models in diabetes researchH James Harwood
Department of Pathology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
J Diabetes Sci Technol 6:503-14. 2012..For these reasons, NHPs are particularly valuable animal models of obesity and diabetes for studying disease pathogenesis, risk factors, comorbidities, and therapeutic interventions...
- Epigenetic changes with dietary soy in cynomolgus monkeysTimothy D Howard
Center for Genomics and Personalized Medicine Research, Wake Forest School of Medicine, Winston Salem, North Carolina, United States of America
PLoS ONE 6:e26791. 2011..These data support the use of the HumanMethylation27 BeadChip in cynomolgus monkeys and identify epigenetic changes associated with dietary interventions with soy protein that may potentially affect the etiology of complex diseases...
- Hydrogen and oxygen isotope ratios in body water and hair: modeling isotope dynamics in nonhuman primatesShannon P O'Grady
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
Am J Primatol 74:651-60. 2012..Additionally, the value of α(ow) was 1.0164, in close agreement with the only other previously measured observation (microbial spore cell walls), suggesting robustness of this fractionation factor across different biological systems...
- Effects of soy protein and isoflavones on insulin resistance and adiponectin in male monkeysJanice D Wagner
Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC 27157 1040, USA
Metabolism 57:S24-31. 2008..Further studies are needed to determine the mechanisms involved in these effects of a high-soy isoflavone diet and to optimize dietary isoflavone content for maximal health benefits in male subjects...
- Effects of soy vs. casein protein on body weight and glycemic control in female monkeys and their offspringJanice D Wagner
Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
Am J Primatol 71:802-11. 2009....
- Genomic diversity and interspecies host infection of alpha12 Macaca fascicularis papillomaviruses (MfPVs)Zigui Chen
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Virology 393:304-10. 2009..Moreover, identification of an overlapping set of MfPVs in rhesus and cynomolgus macaques indicates that non-human primate alpha-PVs might not be strictly species-specific and may represent past interspecies infection...