CSHL CANCER CENTER SUPPORT GRANT

Summary

Principal Investigator: BRUCE W STILLMAN
Abstract: PROJECT SUMMARY (See instructions): The Cold Spring Harbor Laboratory (CSHL) Cancer Center (www.cshl.edu/cancercenter) is a vibrant and dynamic cancer research center that strives for excellence. Researchers are committed to using a focused, multidisciplinary and collaborative approach to investigate the molecular cellular basis of human cancer with the goal of improving diagnosis and treatment of ail major forms ofthe disease. The CSHL Cancer Center is organized into three Scientific Programs. The Gene Expression &Cell Proliferation Program focuses on the regulation of gene expression, cell division cycle control and chromosome structure, comparing normal and cancer cells with a goal to identify new therapeutic targets. The Signal Transduction Program focuses on signal transduction pathways and cell architecture in normal and cancer cells, with a growing emphasis on tumor micro-environment and understanding mechanisms of resistance to targeted therapies. The Cancer Genetics Program focuses on understanding the genetic basis of cancer, tumor progression and discovery of new targets for therapy using innovative mouse models for human cancer. In addition, the CSHL Cancer Center supports ten scientific shared resources. These shared resources provide access to technologies, products, services and expertise that promote multidisciplinary interactions and collaborations among CSHL researchers and programs. Importantly, the shared resources increase productivity, provide economies of scale, decrease wasteful duplication of resources, maintain quality control, and facilitate access to expensive equipment and highly skilled technical services. In the last five years, the CSHL Cancer Center not only broke new ground in understanding cancer genetics and tumor biology, but also achieved its vision of a cancer discovery pipeline that integrates complex cellular processes and pathways in tumor cells with cell biology and biochemistry, human cancer genetics, RNAi technology and innovative animal models that mimic different subsets of human cancers. The pipeline has generated a wealth of pre-clinical data including information on new cancer genes and diagnostic tools, has identified potential new therapeutic targets, has investigated new biochemical pathways, and studied drug resistance mechanisms.
Funding Period: 1997-08-01 - 2016-07-31
more information: NIH RePORT

Top Publications

  1. pmc Acquired dependence of acute myeloid leukemia on the DEAD-box RNA helicase DDX5
    Anthony Mazurek
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell Rep 7:1887-99. 2014
  2. ncbi Translating next generation sequencing to practice: opportunities and necessary steps
    Sitharthan Kamalakaran
    Philips Research North America, Briarcliff Manor, NY 10510, USA
    Mol Oncol 7:743-55. 2013
  3. pmc The making of a slicer: activation of human Argonaute-1
    Christopher R Faehnle
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell Rep 3:1901-9. 2013
  4. pmc A tool to evaluate correspondence between extraction ion chromatographic peaks and peptide-spectrum matches in shotgun proteomics experiments
    Cristian I Ruse
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proteomics 13:2386-97. 2013
  5. pmc Selective inhibition of tumor oncogenes by disruption of super-enhancers
    Jakob Lovén
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 153:320-34. 2013
  6. pmc The anti-inflammatory compound BAY-11-7082 is a potent inhibitor of protein tyrosine phosphatases
    Navasona Krishnan
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 2208, USA
    FEBS J 280:2830-41. 2013
  7. pmc Dogma derailed: the many influences of RNA on the genome
    Leah R Sabin
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 49:783-94. 2013
  8. pmc Dicer-2 processes diverse viral RNA species
    Leah R Sabin
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e55458. 2013
  9. pmc Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia
    Eric Wang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 110:3901-6. 2013
  10. pmc Multiple roles for Piwi in silencing Drosophila transposons
    Nikolay V Rozhkov
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 27:400-12. 2013

Detail Information

Publications57

  1. pmc Acquired dependence of acute myeloid leukemia on the DEAD-box RNA helicase DDX5
    Anthony Mazurek
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell Rep 7:1887-99. 2014
    ..By inhibiting DDX5 expression in vivo, we show that DDX5 is dispensable for normal hematopoiesis and tissue homeostasis. These results validate DDX5 as a potential target for blocking AML...
  2. ncbi Translating next generation sequencing to practice: opportunities and necessary steps
    Sitharthan Kamalakaran
    Philips Research North America, Briarcliff Manor, NY 10510, USA
    Mol Oncol 7:743-55. 2013
    ....
  3. pmc The making of a slicer: activation of human Argonaute-1
    Christopher R Faehnle
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell Rep 3:1901-9. 2013
    ..Intriguingly, the elements that make Argonaute an active slicer involve a sophisticated interplay between the active site and more distant regions of the enzyme. ..
  4. pmc A tool to evaluate correspondence between extraction ion chromatographic peaks and peptide-spectrum matches in shotgun proteomics experiments
    Cristian I Ruse
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proteomics 13:2386-97. 2013
    ..We illustrate analysis of ion chromatograms detected in two different modes by RF linear ion trap and electrostatic field orbitrap...
  5. pmc Selective inhibition of tumor oncogenes by disruption of super-enhancers
    Jakob Lovén
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 153:320-34. 2013
    ..Super-enhancers were found at key oncogenic drivers in many other tumor cells. These observations have implications for the discovery of cancer therapeutics directed at components of super-enhancers in diverse tumor types...
  6. pmc The anti-inflammatory compound BAY-11-7082 is a potent inhibitor of protein tyrosine phosphatases
    Navasona Krishnan
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 2208, USA
    FEBS J 280:2830-41. 2013
    ..Furthermore, the data suggest that inhibition of PTP function may contribute to the many biological effects of BAY-11-7082 that have been reported to date...
  7. pmc Dogma derailed: the many influences of RNA on the genome
    Leah R Sabin
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Mol Cell 49:783-94. 2013
    ..In understanding the mechanisms of RNA control over DNA, the power of RNA may one day be harnessed to impact gene expression in a therapeutic setting...
  8. pmc Dicer-2 processes diverse viral RNA species
    Leah R Sabin
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e55458. 2013
    ..This study uncovered several novel, heavily targeted features within viral genomes, offering insight into viral replication, viral immune evasion strategies, and the mechanism of antiviral RNAi...
  9. pmc Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia
    Eric Wang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 110:3901-6. 2013
    ....
  10. pmc Multiple roles for Piwi in silencing Drosophila transposons
    Nikolay V Rozhkov
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 27:400-12. 2013
    ..Overall, our results indicate that Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis...
  11. pmc Next-generation sequencing of endoscopic biopsies identifies ARID1A as a tumor-suppressor gene in Barrett's esophagus
    M M Streppel
    1 Department of Pathology, John Hopkins Medical Institutions, Baltimore, MD, USA 2 Department of Gastroenterology and Hepatology, University Medical Center Utrecht, Utrecht, The Netherlands 3 Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Oncogene 33:347-57. 2014
    ..Further, we identify ARID1A as a novel tumor-suppressor gene in BE pathogenesis, reiterating the importance of aberrant chromatin in the metaplasia-dysplasia sequence. ..
  12. pmc The piRNA pathway in flies: highlights and future directions
    Paloma M Guzzardo
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States
    Curr Opin Genet Dev 23:44-52. 2013
    ..Here, we discuss these recent findings, as well as highlight some aspects of piRNA biology that continue to escape our understanding...
  13. pmc Chromatin organization and transcriptional regulation
    Michael R Hübner
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, United States
    Curr Opin Genet Dev 23:89-95. 2013
    ..We highlight new findings on the dynamic positioning of genes relative to each other, as well as to their chromosome territory and the nuclear lamina, and how the position of genes correlates with their transcriptional activity...
  14. pmc Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells
    Gaofeng Fan
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Biol Chem 288:24923-34. 2013
    ..These data reveal an important role for PTP1B as a negative regulator of BRK and IGF-1Rβ signaling in ovarian cancer cells. ..
  15. pmc Parallel comparison of Illumina RNA-Seq and Affymetrix microarray platforms on transcriptomic profiles generated from 5-aza-deoxy-cytidine treated HT-29 colon cancer cells and simulated datasets
    Xiao Xu
    School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA
    BMC Bioinformatics 14:S1. 2013
    ..The objective of this study was to compare parallel paired-end RNA-Seq and microarray data generated on 5-azadeoxy-cytidine (5-Aza) treated HT-29 colon cancer cells with an additional simulation study...
  16. pmc Metabolic profiling of dividing cells in live rodent brain by proton magnetic resonance spectroscopy (1HMRS) and LCModel analysis
    June Hee Park
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS ONE 9:e94755. 2014
    ..30 ppm. In addition, we also apply the same 1HMRS methodology to metabolically profile glioblastomas with actively dividing cells growing in RCAS-PDGF mice...
  17. pmc Chd5 orchestrates chromatin remodelling during sperm development
    Wangzhi Li
    1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA 2 Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, New York 11794, USA
    Nat Commun 5:3812. 2014
    ..These findings define Chd5 as a multi-faceted mediator of histone-to-protamine replacement and depict the cascade of molecular events underlying this process of extensive chromatin remodelling. ..
  18. pmc PTEN action in leukaemia dictated by the tissue microenvironment
    Cornelius Miething
    1 Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA 2 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA 3 Department of Medicine I, Medical Center University of Freiburg, 79106 Freiburg, Germany
    Nature 510:402-6. 2014
    ..They further reveal that the role of PTEN loss in tumour maintenance is not invariant and can be influenced by the tissue microenvironment, thereby producing a form of intratumoral heterogeneity that is independent of cancer genotype. ..
  19. pmc Random monoallelic expression: regulating gene expression one allele at a time
    Mélanie A Eckersley-Maslin
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Trends Genet 30:237-44. 2014
    ..Monoallelic gene expression is likely generated through stochastic independent regulation of the two alleles upon differentiation, and has varied implications for the cell and organism, in particular with respect to disease. ..
  20. pmc Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression
    Yi Jun Sheu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724
    Proc Natl Acad Sci U S A 111:E1899-908. 2014
    ..Together, our data suggest that the eukaryotic MCM helicase contains an intrinsic regulatory domain that integrates multiple signals to coordinate origin activation and replication fork progression under stress conditions. ..
  21. pmc miRNAs trigger widespread epigenetically activated siRNAs from transposons in Arabidopsis
    Kate M Creasey
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 508:411-5. 2014
    ..This ancient recognition mechanism may have been retained both by transposons to evade long-term heterochromatic silencing and by their hosts for genome defence. ..
  22. pmc Extended effect of chronic social defeat stress in childhood on behaviors in adulthood
    Irina L Kovalenko
    Institute of Cytology and Genetics SD RAS, Novosibirsk, Russia
    PLoS ONE 9:e91762. 2014
    ..Furthermore, these males were more aggressive in the agonistic interactions. Thus, hostile social environment in adolescence disturbs psychoemotional state and social behaviors of animals in adult life. ..
  23. pmc Random monoallelic gene expression increases upon embryonic stem cell differentiation
    Mélanie A Eckersley-Maslin
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA Watson School of Biological Sciences, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Dev Cell 28:351-65. 2014
    ..These results support a model in which random monoallelic expression occurs stochastically during differentiation and, for some genes, is compensated for by the cell to maintain the required transcriptional output of these genes. ..
  24. pmc Long non-coding RNAs: modulators of nuclear structure and function
    Jan H Bergmann
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Curr Opin Cell Biol 26:10-8. 2014
    ..Here we review recent studies that characterize the molecular function of a subset of these molecules in the regulation and fine-tuning of nuclear state. ..
  25. pmc CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing
    Canan Kuscu
    W M Keck Structural Biology Laboratory, Cold Spring Harbor, NY 11724
    Proc Natl Acad Sci U S A 111:1795-800. 2014
    ..Functional mapping of the putative target-binding surface of Dos1 identifies key residues required for heterochromatic silencing, consistent with Dos1's role as the specificity factor for the E3 ubiquitin ligase. ..
  26. pmc Splicing factor SRSF6 promotes hyperplasia of sensitized skin
    Mads A Jensen
    1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA 2
    Nat Struct Mol Biol 21:189-97. 2014
    ..Furthermore, the effects of SRSF6 in wound healing assayed in vitro depend on the tenascin-C isoforms. Thus, abnormal SR-protein expression can perturb tissue homeostasis. ..
  27. pmc Receptor protein-tyrosine phosphatase α regulates focal adhesion kinase phosphorylation and ErbB2 oncoprotein-mediated mammary epithelial cell motility
    Benoit Boivin
    From the Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
    J Biol Chem 288:36926-35. 2013
    ..Collectively, these results reveal a new role for PTPα in the regulation of motility of mammary epithelial cells in response to ErbB2 activation. ..
  28. doi Inducing RNAi in C. elegans by feeding with dsRNA-expressing E. coli
    Christopher M Hammell
    Cold Spring Harb Protoc 2012:. 2012
    ..coli in liquid culture (commonly used to score effects of RNAi on postembryonic larval development) and another involving feeding of late larval- or adult-staged animals on standard solid medium culture plates...
  29. pmc Protein tyrosine phosphatases--from housekeeping enzymes to master regulators of signal transduction
    Nicholas K Tonks
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 2208, USA
    FEBS J 280:346-78. 2013
    ..I have discussed various aspects of the structure, regulation and function of the protein tyrosine phosphatase family, which I hope will illustrate the fundamental importance of these enzymes in the control of signal transduction...
  30. pmc Unique small RNA signatures uncovered in the tammar wallaby genome
    James Lindsay
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    BMC Genomics 13:559. 2012
    ....
  31. pmc Epithelial cell organization suppresses Myc function by attenuating Myc expression
    David R Simpson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Cancer Res 71:3822-30. 2011
    ..These observations reveal that organization does not alter the inherent response of epithelial cells to Myc and suggest that other tumor suppression mechanisms, apart from structure, antagonize Myc in the development of solid tumors...
  32. pmc SFSSClass: an integrated approach for miRNA based tumor classification
    Ramkrishna Mitra
    Machine Intelligence Unit, Indian Statistical Institute, Kolkata, India
    BMC Bioinformatics 11:S22. 2010
    ..A judicious integration of such knowledge with effective miRNA and sample selection through a biclustering approach could be an important step in improving the accuracy of tumor classification...
  33. pmc Population-genetic nature of copy number variations in the human genome
    Mamoru Kato
    Center for Genomic Medicine, RIKEN, 1 7 22 Suehiro, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Hum Mol Genet 19:761-73. 2010
    ..After further investigation of tag SNPs for CNVs, we conclude that the customary tagging strategy for disease association studies can be applicable for common deletion CNVs, but direct interrogation is needed for other types of CNVs...
  34. pmc An integrative genomics approach identifies Hypoxia Inducible Factor-1 (HIF-1)-target genes that form the core response to hypoxia
    Yair Benita
    Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Nucleic Acids Res 37:4587-602. 2009
    ..Together these analyses demonstrate the potential to recover novel HIF-1-target genes and the discovery of mammalian-regulatory elements operative in the context of microarray data sets...
  35. pmc Dephosphorylation of the C-terminal tyrosyl residue of the DNA damage-related histone H2A.X is mediated by the protein phosphatase eyes absent
    Navasona Krishnan
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Biol Chem 284:16066-70. 2009
    ..X in vivo. This study provides the first indication of a physiological substrate for the EYA phosphatases and suggests a novel role for these enzymes in regulation of the DNA damage response...
  36. pmc Inference of cell cycle-dependent proteolysis by laser scanning cytometry
    Abhishek A Chakraborty
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Exp Cell Res 315:1772-8. 2009
    ....
  37. pmc A modified cysteinyl-labeling assay reveals reversible oxidation of protein tyrosine phosphatases in angiomyolipoma cells
    Benoit Boivin
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 2208, USA
    Proc Natl Acad Sci U S A 105:9959-64. 2008
    ..We anticipate that this cysteinyl-labeling enrichment strategy can be applied broadly to study reversible oxidation as a mechanism of harnessing PTP catalytic activity in a variety of signaling pathways...
  38. pmc Adenovirus E1A targets p400 to induce the cellular oncoprotein Myc
    Kathryn A Tworkowski
    Cold Spring Harbor Laboratory, and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 105:6103-8. 2008
    ..Together, these findings establish that Myc, via p400, is an essential downstream target of E1A...
  39. ncbi Genetic similarities between organogenesis and tumorigenesis of the lung
    Scott Powers
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, New York USA
    Cell Cycle 7:200-4. 2008
    ....
  40. pmc Oncogenic cooperation and coamplification of developmental transcription factor genes in lung cancer
    Jude Kendall
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 104:16663-8. 2007
    ....
  41. pmc Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF(65)
    Michelle L Hastings
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America
    PLoS ONE 2:e538. 2007
    ..Our results indicate that recognition of 3' splice sites involves different U2AF-like molecules, and that modulation of these general splicing factors can have profound effects on splicing...
  42. pmc Identification of PTPN23 as a novel regulator of cell invasion in mammary epithelial cells from a loss-of-function screen of the 'PTP-ome'
    Guang Lin
    Graduate Program in Molecular and Cellular Biology, Stony Brook University, Stony Brook, New York 11794, USA
    Genes Dev 25:1412-25. 2011
    ..In addition, our studies highlight functional specificity among PTPs and reveal new roles for PTPs in mammary epithelial cell biology...
  43. pmc H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response
    Navasona Krishnan
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Sci Signal 4:ra86. 2011
    ..Together, these data define a signaling function for H(2)S in inhibiting PTP1B activity and thereby promoting PERK activity during the response to ER stress...
  44. pmc The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis
    Jonathan J Ipsaro
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nature 491:279-83. 2012
    ..Together, our data suggest that the Zucchini proteins act in primary piRNA biogenesis as nucleases, perhaps generating the 5' ends of primary piRNAs...
  45. pmc Extreme telomere length dimorphism in the Tasmanian devil and related marsupials suggests parental control of telomere length
    Hannah S Bender
    Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia
    PLoS ONE 7:e46195. 2012
    ..Comparative studies in five distantly related marsupials and a monotreme indicate that telomere dimorphism evolved at least 50 million years ago...
  46. pmc Site identification in high-throughput RNA-protein interaction data
    Philip J Uren
    Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA
    Bioinformatics 28:3013-20. 2012
    ..Statistically robust, flexible computational methods for binding site identification from high-throughput immunoprecipitation assays are largely lacking however...
  47. pmc Landscape of transcription in human cells
    Sarah Djebali
    Centre for Genomic Regulation and UPF, Doctor Aiguader 88, Barcelona 08003, Catalonia, Spain
    Nature 489:101-8. 2012
    ..These observations, taken together, prompt a redefinition of the concept of a gene...
  48. pmc An integrated encyclopedia of DNA elements in the human genome
    Bradley E Bernstein
    Vertebrate Genomics Group, European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK
    Nature 489:57-74. 2012
    ..Overall, the project provides new insights into the organization and regulation of our genes and genome, and is an expansive resource of functional annotations for biomedical research...
  49. pmc The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult
    Bin Zhang
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell Rep 2:111-23. 2012
    ..However, among a small number of genes that were dysregulated in adult Malat1 knockout mice, many were Malat1 neighboring genes, thus indicating a potential cis-regulatory role of Malat1 gene transcription...
  50. pmc DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells
    Anthony Mazurek
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Cancer Discov 2:812-25. 2012
    ..Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment...
  51. pmc MicroRNA93 regulates proliferation and differentiation of normal and malignant breast stem cells
    Suling Liu
    Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    PLoS Genet 8:e1002751. 2012
    ..These studies demonstrate that miRNAs can regulate the states and fates of normal and malignant mammary stem cells, findings which have important biological and clinical implications...
  52. pmc The structure of human argonaute-2 in complex with miR-20a
    Elad Elkayam
    W M Keck Structural Biology Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 150:100-10. 2012
    ..Interestingly, miRNA binding confers remarkable stability on hAgo2, locking this otherwise flexible enzyme into a stable conformation...
  53. pmc Functional analysis of the protein phosphatase activity of PTEN
    Xiaoqun Catherine Zhang
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Biochem J 444:457-64. 2012
    ....
  54. pmc A pipeline for the generation of shRNA transgenic mice
    Lukas E Dow
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
    Nat Protoc 7:374-93. 2012
    ..In all, this 14-week procedure provides a fast and cost-effective way for any laboratory to investigate gene function in vivo in the mouse...
  55. ncbi CHD5 is a tumor suppressor at human 1p36
    Anindya Bagchi
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell 128:459-75. 2007
    ..We demonstrate that Chd5 functions as a tumor suppressor in vivo and implicate deletion of CHD5 in human cancer. Identification of this tumor suppressor provides new avenues for exploring innovative clinical interventions for cancer...