Gene Symbol: you
Description: signal peptide, CUB domain, EGF-like 2
Alias: wu:fc27f08, you, signal peptide, CUB and EGF-like domain-containing protein 2, U shaped somites, protein You, u-shaped somites
Species: zebrafish
Products:     you

Top Publications

  1. Chen J, Haffter P, Odenthal J, Vogelsang E, Brand M, van Eeden F, et al. Mutations affecting the cardiovascular system and other internal organs in zebrafish. Development. 1996;123:293-302 pubmed
    ..The mutations presented here could serve as an entry point to the establishment of a genetic hierarchy underlying organogenesis. ..
  2. Lewis K, Currie P, Roy S, Schauerte H, Haffter P, Ingham P. Control of muscle cell-type specification in the zebrafish embryo by Hedgehog signalling. Dev Biol. 1999;216:469-80 pubmed
  3. Stickney H, Barresi M, Devoto S. Somite development in zebrafish. Dev Dyn. 2000;219:287-303 pubmed
    ..We show directly, for the first time, that muscle cell and sclerotome migrations occur at the same time. We end with a look at the many questions about somitogenesis that are still unanswered. ..
  4. Woods I, Talbot W. The you gene encodes an EGF-CUB protein essential for Hedgehog signaling in zebrafish. PLoS Biol. 2005;3:e66 pubmed
    ..Large-scale genetic screens in zebrafish have identified a group of mutations, termed you-class mutations, that share common defects in somite shape and in most cases disrupt Hedgehog signaling...
  5. Kawakami A, Nojima Y, Toyoda A, Takahoko M, Satoh M, Tanaka H, et al. The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling. Curr Biol. 2005;15:480-8 pubmed
    ..We report that the gene disrupted in zebrafish you mutants, in which Hh signaling is impaired, encodes the secreted matrix protein Scube2...
  6. Ingham P, Kim H. Hedgehog signalling and the specification of muscle cell identity in the zebrafish embryo. Exp Cell Res. 2005;306:336-42 pubmed
    ..Distinct muscle cell identities are induced by varying levels of signalling activity. The SET domain transcription factor, Blimp1, is a key target of Hedgehog signalling in this process. ..
  7. Hollway G, Maule J, Gautier P, Evans T, Keenan D, Lohs C, et al. Scube2 mediates Hedgehog signalling in the zebrafish embryo. Dev Biol. 2006;294:104-18 pubmed
    ..Formation of Hedgehog-sensitive muscle fates is disrupted within homozygous zebrafish mutants of the "you"-type class, the majority of which disrupt components of the Hedgehog (HH) signal transduction pathway...
  8. Johnson J, Hall T, Dyson J, Sonntag C, Ayers K, Berger S, et al. Scube activity is necessary for Hedgehog signal transduction in vivo. Dev Biol. 2012;368:193-202 pubmed publisher
    ..We further define the molecular role of scube2 in HH signaling. ..
  9. Hadzhiev Y, Lele Z, Schindler S, Wilson S, Ahlberg P, Strahle U, et al. Hedgehog signaling patterns the outgrowth of unpaired skeletal appendages in zebrafish. BMC Dev Biol. 2007;7:75 pubmed
    ..The median fins evolved before paired fins in early craniates, thus the patterning of the median fins may be an ancestral mechanism that controls the outgrowth of skeletogenic appendages in vertebrates. ..

More Information


  1. Tsai M, Cheng C, Lin Y, Chen C, Wu A, Wu M, et al. Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans. Biochem J. 2009;422:119-28 pubmed publisher
    ..Thus human SCUBE2 could play important roles in HH-related biology and pathology, such as during organ development and tumour progression. ..
  2. Creanga A, Glenn T, Mann R, Saunders A, Talbot W, Beachy P. Scube/You activity mediates release of dually lipid-modified Hedgehog signal in soluble form. Genes Dev. 2012;26:1312-25 pubmed publisher
    ..Yet Hh proteins are also capable of mobilizing to and eliciting direct responses from distant cells. The zebrafish you gene, identified genetically >15 years ago, was more recently shown to encode a secreted glycoprotein that acts ..
  3. Tsao K, Tu C, Lee S, Yang R. Zebrafish scube1 (signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 1) is involved in primitive hematopoiesis. J Biol Chem. 2013;288:5017-26 pubmed publisher
    ..Our results suggest that scube1 is critical for and functions at the top of the regulatory hierarchy of primitive hematopoiesis by modulating BMP activity during zebrafish embryogenesis. ..
  4. Corallo D, Schiavinato A, Trapani V, Moro E, Argenton F, Bonaldo P. Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development. 2013;140:4594-601 pubmed publisher
    ..Overall, this study reveals a new role for an EMILIN protein and reinforces the concept that structure and function of the notochord are strictly linked. ..