Gene Symbol: vegfaa
Description: vascular endothelial growth factor Aa
Alias: vegf, vegfa, wu:fj82c06, vascular endothelial growth factor A-A, VEGF-A-A, etID309976.16, vascular endothelial growth factor A, vegf-a
Species: zebrafish
Products:     vegfaa

Top Publications

  1. Nasevicius A, Larson J, Ekker S. Distinct requirements for zebrafish angiogenesis revealed by a VEGF-A morphant. Yeast. 2000;17:294-301 pubmed
    ..developmental process that requires signalling by the secreted protein vascular endothelial growth factor-A (VEGF-A)...
  2. Herbert S, Huisken J, Kim T, Feldman M, Houseman B, Wang R, et al. Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation. Science. 2009;326:294-8 pubmed publisher
    ..Thus, directional control of progenitor migration drives arterial-venous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development. ..
  3. Jin S, Beis D, Mitchell T, Chen J, Stainier D. Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development. 2005;132:5199-209 pubmed
    ..Downregulating the VEGF signaling pathway, while affecting the number of angioblasts, does not appear to affect their migratory behavior...
  4. van Rooijen E, Voest E, Logister I, Bussmann J, Korving J, van Eeden F, et al. von Hippel-Lindau tumor suppressor mutants faithfully model pathological hypoxia-driven angiogenesis and vascular retinopathies in zebrafish. Dis Model Mech. 2010;3:343-53 pubmed publisher
    ..Real-time quantitative PCR revealed increased expression of the duplicated VEGFA orthologs vegfaa and vegfab, and of vegfb and its receptors flt1, kdr and kdr-like, indicating increased vascular endothelial ..
  5. Ding C, Zhang J, Zhao Y, Peng Z, Song D, Jiang J. Zebrafish as a potential model organism for drug test against hepatitis C virus. PLoS ONE. 2011;6:e22921 pubmed publisher
    ..Thus, zebrafish might be a model organism to host HCV, and this zebrafish/HCV (sub-replicon) system could be an animal model for anti-HCV drug screening and evaluation. ..
  6. Covassin L, Siekmann A, Kacergis M, Laver E, Moore J, Villefranc J, et al. A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Dev Biol. 2009;329:212-26 pubmed publisher
    ..Subsequent characterization of mutant lines revealed that defects in Vascular endothelial growth factor (Vegf) signaling specifically affected artery development...
  7. Lawson N, Vogel A, Weinstein B. sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation. Dev Cell. 2002;3:127-36 pubmed
    ..We find that vascular endothelial growth factor (vegf) acts downstream of sonic hedgehog (shh) and upstream of the Notch pathway to determine arterial cell fate...
  8. Liang D, Chang J, Chin A, Smith A, Kelly C, Weinberg E, et al. The role of vascular endothelial growth factor (VEGF) in vasculogenesis, angiogenesis, and hematopoiesis in zebrafish development. Mech Dev. 2001;108:29-43 pubmed
    Vascular endothelial growth factor (VEGF, VEGF-A), a selective mitogen for endothelial cells is a critical factor for vascular development...
  9. So J, Hong S, Kim H, Jung S, Lee M, Choi J, et al. Gicerin/Cd146 is involved in zebrafish cardiovascular development and tumor angiogenesis. Genes Cells. 2010;15:1099-110 pubmed publisher
    ..Mechanistically, the angiogenic function of gicerin was found to be genetically linked to VEGF signaling in the knock-down experiments using vegf-a mRNA, VEGFR inhibitor and gicerin morpholino...

More Information


  1. Wiley D, Kim J, Hao J, Hong C, Bautch V, Jin S. Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein. Nat Cell Biol. 2011;13:686-92 pubmed publisher
    ..We found that angiogenic sprouts from the dorsal aorta are dependent on vascular endothelial growth factor A (Vegf-A) signalling, and do not respond to bone morphogenetic protein (Bmp) signals...
  2. Jing C, Chen Y, Dong M, Peng X, Jia X, Gao L, et al. Phospholipase C gamma-1 is required for granulocyte maturation in zebrafish. Dev Biol. 2013;374:24-31 pubmed publisher
    ..These results suggested that plcg1 functioned specifically in zebrafish ICM granulopoiesis for the first time. Our studies suggest that specific pathways regulate the differentiation of the hematopoietic lineages. ..
  3. Bahary N, Goishi K, Stuckenholz C, Weber G, Leblanc J, Schafer C, et al. Duplicate VegfA genes and orthologues of the KDR receptor tyrosine kinase family mediate vascular development in the zebrafish. Blood. 2007;110:3627-36 pubmed
    b>Vascular endothelial growth factor A (VEGFA) and the type III receptor tyrosine kinase receptors (RTKs) are both required for the differentiation of endothelial cells (vasculogenesis) and for the sprouting of new capillaries (angiogenesis)..
  4. Rottbauer W, Just S, Wessels G, Trano N, Most P, Katus H, et al. VEGF-PLCgamma1 pathway controls cardiac contractility in the embryonic heart. Genes Dev. 2005;19:1624-34 pubmed
    ..In other situations, PLCgamma1 is known to transduce the signal from vascular endothelial growth factor (VEGF), and we show here that abrogation of VEGF also interferes with cardiac contractility...
  5. Covassin L, Villefranc J, Kacergis M, Weinstein B, Lawson N. Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish. Proc Natl Acad Sci U S A. 2006;103:6554-9 pubmed
    Recent evidence indicates a specific role for vascular endothelial growth factor a (Vegfa) during artery development in both zebrafish and mouse embryos, whereas less is known about signals that govern vein formation...
  6. Cermenati S, Moleri S, Cimbro S, Corti P, Del Giacco L, Amodeo R, et al. Sox18 and Sox7 play redundant roles in vascular development. Blood. 2008;111:2657-66 pubmed
    ..Our data suggest that a defect in arteriovenous identity could be responsible for the formation of telangiectases in patients with HLT. ..
  7. Bayliss P, Bellavance K, Whitehead G, Abrams J, Aegerter S, Robbins H, et al. Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish. Nat Chem Biol. 2006;2:265-73 pubmed
    ..This study illustrates the utility of the adult zebrafish as a new model system for receptor signaling and chemical biology. ..
  8. Jensen L, Cao Z, Nakamura M, Yang Y, Bräutigam L, Andersson P, et al. Opposing effects of circadian clock genes bmal1 and period2 in regulation of VEGF-dependent angiogenesis in developing zebrafish. Cell Rep. 2012;2:231-41 pubmed publisher
    ..Using a promoter-reporter system consisting of various deleted vegf-promoter mutants, we show that Bmal1 directly binds to and activates the vegf promoter via E-boxes...
  9. Goishi K, Klagsbrun M. Vascular endothelial growth factor and its receptors in embryonic zebrafish blood vessel development. Curr Top Dev Biol. 2004;62:127-52 pubmed
    ..The vascular endothelial growth factor (VEGF) and its receptors are major contributors to normal mammalian vascular development...
  10. Lawson N, Mugford J, Diamond B, Weinstein B. phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Genes Dev. 2003;17:1346-51 pubmed
    ..We further show that plcg1y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic ..
  11. Weijts B, Bakker W, Cornelissen P, Liang K, Schaftenaar F, Westendorp B, et al. E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1. EMBO J. 2012;31:3871-84 pubmed publisher
    ..provide evidence for a molecular mechanism through which E2F7/8 activate the transcription of the vascular endothelial growth factor A (VEGFA), a key factor in guiding angiogenesis...
  12. Childs S, Chen J, Garrity D, Fishman M. Patterning of angiogenesis in the zebrafish embryo. Development. 2002;129:973-82 pubmed
    ..The dorsal extent of the ISV is less perturbed. Precocious sprouting can be inhibited in a VEGF morphant, but the anomalous site of origin of obd ISVs remains...
  13. Meder B, Huttner I, Sedaghat Hamedani F, Just S, Dahme T, Frese K, et al. PINCH proteins regulate cardiac contractility by modulating integrin-linked kinase-protein kinase B signaling. Mol Cell Biol. 2011;31:3424-35 pubmed publisher
    ..Inactivation of either PINCH isoform independently leads to instability of ILK, loss of stretch-responsive anf and vegf expression, and progressive heart failure...
  14. Diamante G, do Amaral E Silva Müller G, Menjivar Cervantes N, Xu E, Volz D, Dias Bainy A, et al. Developmental toxicity of hydroxylated chrysene metabolites in zebrafish embryos. Aquat Toxicol. 2017;189:77-86 pubmed publisher
    ..Our findings also demonstrate the regio-selective toxicity of hydroxy-PAHs in the effects on eye and circulatory development and raise the need to identify mechanisms and ecological risks of oxy-PAHs to fish populations. ..
  15. Zhu C, Smith T, MCNULTY J, Rayla A, Lakshmanan A, Siekmann A, et al. Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011;138:4555-64 pubmed publisher
    ..This work provides a resource to allow targeted germline gene inactivation in zebrafish and highlights the benefit of a definitive reverse genetic strategy to reveal gene function. ..
  16. Bloomekatz J, Singh R, Prall O, Dunn A, Vaughan M, Loo C, et al. Platelet-derived growth factor (PDGF) signaling directs cardiomyocyte movement toward the midline during heart tube assembly. elife. 2017;6: pubmed publisher
    ..Together, these data uncover a novel mechanism through which endodermal-myocardial communication can guide the cell movements that initiate cardiac morphogenesis. ..
  17. Minehata K, Kawahara A, Suzuki T. meis1 regulates the development of endothelial cells in zebrafish. Biochem Biophys Res Commun. 2008;374:647-52 pubmed publisher
    ..The signaling pathways mediated by vascular endothelial growth factor (vegf) are required for these processes...
  18. Stahlhut C, Suarez Y, Lu J, Mishima Y, Giraldez A. miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development. 2012;139:4356-64 pubmed publisher
    ..Using target protectors, our results indicate that miR-1/206 directly regulate the levels of Vascular endothelial growth factor A (VegfA) in muscle, controlling the strength of angiogenic signaling to the endothelium...
  19. Chun C, Remadevi I, Schupp M, Samant G, Pramanik K, Wilkinson G, et al. Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish. PLoS ONE. 2011;6:e14732 pubmed publisher
    ..The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components ..
  20. Yu X, Tong Y, Kwok H, Sze S, Zhong L, Lau C, et al. Anti-angiogenic activity of Erxian Decoction, a traditional Chinese herbal formula, in zebrafish. Biol Pharm Bull. 2012;35:2119-27 pubmed
    ..Furthermore, it reduced the mRNA expression of vascular endothelial growth factor A (VEGF-A) and the protein level of hypoxia inducible factor 1? (HIF-1?) in the embryos, suggesting ..
  21. Noren D, Chou W, Lee S, Qutub A, Warmflash A, Wagner D, et al. Endothelial cells decode VEGF-mediated Ca2+ signaling patterns to produce distinct functional responses. Sci Signal. 2016;9:ra20 pubmed publisher
    ..We examined Ca(2+) signaling in response to VEGF (vascular endothelial growth factor), a growth factor that can stimulate different behaviors in endothelial cells...
  22. Chang S, Lu Y, Li X, Hsieh W, Xiong Y, Ghosh M, et al. Antagonistic function of the RNA-binding protein HuR and miR-200b in post-transcriptional regulation of vascular endothelial growth factor-A expression and angiogenesis. J Biol Chem. 2013;288:4908-21 pubmed publisher
    ..One such mRNA encodes vascular endothelial growth factor (VEGF)-A, a major regulator of angiogenesis...
  23. Parente V, Balasso S, Pompilio G, Verduci L, Colombo G, Milano G, et al. Hypoxia/reoxygenation cardiac injury and regeneration in zebrafish adult heart. PLoS ONE. 2013;8:e53748 pubmed publisher
    ..The initial decrease in ventricular function is followed by full recovery. This model more closely mimics reperfusion injury in mammals than other cardiac injury models. ..
  24. Koenig A, Baltrunaite K, Bower N, Rossi A, Stainier D, Hogan B, et al. Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein. Dev Biol. 2016;411:115-27 pubmed publisher
    ..We show that vascular endothelial growth factor aa (vegfaa) is expressed in the endoderm at the site where intestinal vessels form and therefore likely provides a guidance ..
  25. Hyvärinen J, Parikka M, Sormunen R, Ramet M, Tryggvason K, Kivirikko K, et al. Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromised kidney function. J Biol Chem. 2010;285:42023-32 pubmed publisher
    ..Many HIF target genes were induced in the P4H-TM-deficient morphants, but the observed phenotype is not likely to be mediated at least solely via the HIF pathway, and thus P4H-TM probably has additional, as yet unknown, substrates. ..
  26. Robertson C, Wright P, Köblitz L, Bernier N. Hypoxia-inducible factor-1 mediates adaptive developmental plasticity of hypoxia tolerance in zebrafish, Danio rerio. Proc Biol Sci. 2014;281: pubmed publisher
    ..We conclude that mounting a HIF-1 response during embryogenesis is associated with long-term impacts on the phenotype of later stages which could influence both individual hypoxia tolerance and population dynamics. ..
  27. Lee P, Goishi K, Davidson A, Mannix R, Zon L, Klagsbrun M. Neuropilin-1 is required for vascular development and is a mediator of VEGF-dependent angiogenesis in zebrafish. Proc Natl Acad Sci U S A. 2002;99:10470-5 pubmed
    Neuropilin-1 (NRP1) is a cell-surface receptor for both vascular endothelial growth factor(165) (VEGF(165)) and class 3 semaphorins that is expressed by neurons and endothelial cells...
  28. Goldstone H, Stegeman J. Molecular mechanisms of 2,3,7,8-tetrachlorodibenzo-p-dioxin cardiovascular embryotoxicity. Drug Metab Rev. 2006;38:261-89 pubmed
    ..Gene expression profiling is elucidating additional pathways by which TCDD might act. We review our understanding of the mechanism of TCDD embryotoxicity at morphological and molecular levels. ..
  29. Lacave J, Fanjul Á, Bilbao E, Gutierrez N, Barrio I, Arostegui I, et al. Acute toxicity, bioaccumulation and effects of dietary transfer of silver from brine shrimp exposed to PVP/PEI-coated silver nanoparticles to zebrafish. Comp Biochem Physiol C Toxicol Pharmacol. 2017;199:69-80 pubmed publisher
    ..Overall, these results indicate an effective dietary transfer of silver and point out to liver as the main target organ for Ag NP toxicity in zebrafish after dietary exposure. ..
  30. Jiang X, Huang Y, Wang X, Liang Q, Li Y, Li F, et al. Dianhydrogalactitol, a potential multitarget agent, inhibits glioblastoma migration, invasion, and angiogenesis. Biomed Pharmacother. 2017;91:1065-1074 pubmed publisher
    ..results suggest that DAG may be a potential multitarget agent that can inhibit tumor migration, invasion, and angiogenesis, and the anti-angiogenic effects may be involved in dual-suppression VEGF/VEGFR2 and FGF2/FGFR1 signal pathways.
  31. Casie Chetty S, Rost M, Enriquez J, Schumacher J, Baltrunaite K, Rossi A, et al. Vegf signaling promotes vascular endothelial differentiation by modulating etv2 expression. Dev Biol. 2017;424:147-161 pubmed publisher
    ..etv2 expression was downregulated in Vegfr- inhibited embryos, and expanded in Vegfaa-overexpressing embryos...
  32. Li S, Dang Y, Zhou X, Huang B, Huang X, Zhang Z, et al. Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway. Sci Rep. 2015;5:16815 pubmed publisher
  33. Mouillesseaux K, Chen J. Mutation in utp15 disrupts vascular patterning in a p53-dependent manner in zebrafish embryos. PLoS ONE. 2011;6:e25013 pubmed publisher
    ..Taken together, our data demonstrate an early embryonic effect of Utp15 deficiency on cell survival and the normal patterning of the vasculature and highlight an anti-angiogenic role of p53 in developing embryos. ..
  34. Gore A, Swift M, Cha Y, Lo B, McKinney M, Li W, et al. Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development. 2011;138:4875-86 pubmed publisher
  35. Rissone A, Foglia E, Sangiorgio L, Cermenati S, Nicoli S, Cimbro S, et al. The synaptic proteins ?-neurexin and neuroligin synergize with extracellular matrix-binding vascular endothelial growth factor a during zebrafish vascular development. Arterioscler Thromb Vasc Biol. 2012;32:1563-72 pubmed publisher
    ..1 together or in combination with morpholinos targeting the -heparin--binding isoforms of vascular endothelial growth factor A (encoded by the VEGFAb gene) recapitulates the observed abnormalities, suggesting synergistic ..
  36. Meder B, Just S, Vogel B, Rudloff J, Gärtner L, Dahme T, et al. JunB-CBFbeta signaling is essential to maintain sarcomeric Z-disc structure and when defective leads to heart failure. J Cell Sci. 2010;123:2613-20 pubmed publisher
  37. De Angelis J, Lagendijk A, Chen H, Tromp A, Bower N, Tunny K, et al. Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover. Dev Cell. 2017;40:123-136 pubmed publisher
    ..Vascular endothelial growth factors (VEGFs) regulate angiogenesis by acting through VEGF receptors to induce endothelial cell signaling...
  38. Goi M, Childs S. Patterning mechanisms of the sub-intestinal venous plexus in zebrafish. Dev Biol. 2016;409:114-128 pubmed publisher
    ..Blocking Shh, Notch and Pdgf signaling has no effect on SIVP growth. However Vegf promotes sprouting of the predominantly venous plexus and Bmp promotes outgrowth of the structure...
  39. BURNS C, Galloway J, Smith A, Keefe M, Cashman T, Paik E, et al. A genetic screen in zebrafish defines a hierarchical network of pathways required for hematopoietic stem cell emergence. Blood. 2009;113:5776-82 pubmed publisher
    ..loss-of-function studies in zebrafish, we ordered these signaling pathways with respect to each other and to the Vegf, Notch, and Runx programs...
  40. Zoeller J, Whitelock J, Iozzo R. Perlecan regulates developmental angiogenesis by modulating the VEGF-VEGFR2 axis. Matrix Biol. 2009;28:284-91 pubmed publisher
    ..Because these events are largely mediated by VEGF-VEGFR2 signaling, we investigated the relationship between perlecan and the VEGF pathway...
  41. Chen Y, Chang C, Lai Y, Sun C, Ding Y, Tsai J. von Hippel-Lindau gene plays a role during zebrafish pronephros development. In Vitro Cell Dev Biol Anim. 2015;51:1023-32 pubmed publisher
    ..In addition, we found that vhl morphants displayed elevated expression of vegfa in podocytes and increased angiogenesis at pronephric glomerulus and the nearby vessels...
  42. Lin C, Lee H, Fu C, Ding Y, Chen J, Lee M, et al. MiR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos. Nat Commun. 2013;4:2829 pubmed publisher
    ..results in a failure to develop some blood vessels and a decrease in vascular endothelial growth factor Aa (VegfAa) expression...
  43. Jensen L, Cao Y. Clock controls angiogenesis. Cell Cycle. 2013;12:405-8 pubmed publisher
    ..At the molecular level, we show that the circadian regulator Bmal1 directly targets the promoter region of the vegf gene in zebrafish, leading to an elevated expression of VEGF...
  44. Li W, Chen J, Deng M, Jing Q. The zebrafish Tie2 signaling controls tip cell behaviors and acts synergistically with Vegf pathway in developmental angiogenesis. Acta Biochim Biophys Sin (Shanghai). 2014;46:641-6 pubmed publisher
    ..Although it is known that several pathways including vascular endothelial growth factor (VEGF) and Notch control tip cell behaviors, the signaling process is largely unknown...
  45. Craig P, Hogstrand C, Wood C, McClelland G. Gene expression endpoints following chronic waterborne copper exposure in a genomic model organism, the zebrafish, Danio rerio. Physiol Genomics. 2009;40:23-33 pubmed publisher
    ..5% contained only a consensus MRE. We conclude that the indirect effects of Cu exposure regulate gene expression to a much greater degree than the direct effects. ..
  46. Massarsky A, Prasad G, Di Giulio R. Total particulate matter from cigarette smoke disrupts vascular development in zebrafish brain (Danio rerio). Toxicol Appl Pharmacol. 2018;339:85-96 pubmed publisher
    ..transcript abundance of key angiogenic genes Tie-2, Angpt1, Notch3, and Flk1 remained largely unchanged and that of Vegf actually increased with TPM...
  47. Huang Y, Huang W, Lin W, Chen M, Jeng S. An endothelial-cell-enriched primary culture system to study vascular endothelial growth factor (VEGF A) expression in a teleost, the Japanese eel (Anguilla japonica). Comp Biochem Physiol A Mol Integr Physiol. 2006;145:33-46 pubmed
    ..basic fibroblast growth factor (bFGF), and estradiol (E2), which have been demonstrated to stimulate mammalian VEGF A expression, followed by quantification of the VEGF mRNA levels by real-time reverse transcription polymerase chain ..
  48. Huang W, Wang J, Liang Y, Ge W, Wang G, Li Y, et al. Potent anti-angiogenic component in Croton crassifolius and its mechanism of action. J Ethnopharmacol. 2015;175:185-91 pubmed publisher
    ..acid affected multiple molecular targets related to angiogenesis including vascular endothelial growth factor (Vegfa), angpiopoietin (Angpt), and their receptors...
  49. Helker C, Schuermann A, Pollmann C, Chng S, Kiefer F, Reversade B, et al. The hormonal peptide Elabela guides angioblasts to the midline during vasculogenesis. elife. 2015;4: pubmed publisher
  50. Rossi A, Gauvrit S, Marass M, Pan L, Moens C, Stainier D. Regulation of Vegf signaling by natural and synthetic ligands. Blood. 2016;128:2359-2366 pubmed publisher
    The mechanisms that allow cells to bypass anti-vascular endothelial growth factor A (VEGFA) therapy remain poorly understood...
  51. Bonventre J, Kung T, White L, Cooper K. Manipulation of the HIF-Vegf pathway rescues methyl tert-butyl ether (MTBE)-induced vascular lesions. Toxicol Appl Pharmacol. 2013;273:623-34 pubmed publisher
    ..HIF-driven angiogenesis via vascular endothelial growth factor (vegf) is essential to the developing vasculature of an embryo...
  52. Merrigan S, Kennedy B. Vitamin D receptor agonists regulate ocular developmental angiogenesis and modulate expression of dre-miR-21 and VEGF. Br J Pharmacol. 2017;174:2636-2651 pubmed publisher
    ..Interestingly, zebrafish ocular vegfaa and vegfab expression was significantly increased while, vegfc, flt1 and kdrl expression was unchanged by ..
  53. Mei J, Liu S, Li Z, Gui J. Mtmr8 is essential for vasculature development in zebrafish embryos. BMC Dev Biol. 2010;10:96 pubmed publisher
    ..Here, we attempt to explore the function of Mtmr8 in vasculature development parallel to its function in muscle development...
  54. Ba Q, Duan J, Tian J, Wang Z, Chen T, Li X, et al. Dihydroartemisinin promotes angiogenesis during the early embryonic development of zebrafish. Acta Pharmacol Sin. 2013;34:1101-7 pubmed publisher
    ..The expression of angiogenesis marker genes vegfa, flk1, and flt1 in the embryos was detected using real-time PCR and RNA in situ hybridization assays...
  55. Lam K, Alex D, Lam I, Tsui S, Yang Z, Lee S. Nobiletin, a polymethoxylated flavonoid from citrus, shows anti-angiogenic activity in a zebrafish in vivo model and HUVEC in vitro model. J Cell Biochem. 2011;112:3313-21 pubmed publisher
    ..Nobiletin also dose-dependently induced VEGF-A mRNA expression...
  56. Kim P, Albacker C, Lu Y, Jang I, Lim Y, Heffner G, et al. Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial-to-hematopoietic transition. Proc Natl Acad Sci U S A. 2013;110:E141-50 pubmed publisher
    ..Together, our results reveal that Scl overexpression is sufficient to rescue the developmental defects caused by blocking the Hh and Notch pathways, and inform our understanding of the embryonic endothelial-to-hematopoietic transition. ..
  57. Nathan J, Lakshmanan G, Michael F, Seppan P, Ragunathan M. Expression of adenosine receptors and vegf during angiogenesis and its inhibition by pentoxifylline-A study using zebrafish model. Biomed Pharmacother. 2016;84:1406-1418 pubmed publisher
    ..2 hpf) of zebrafish embryos and studied the mRNA expression of major angiogenic factors like vegfaa and its receptors under normal conditions and when treated with an adenosine analog NECA (5'-N-..
  58. Peterson R, Shaw S, Peterson T, Milan D, Zhong T, Schreiber S, et al. Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation. Nat Biotechnol. 2004;22:595-9 pubmed
    ..They act to upregulate expression of vascular endothelial growth factor (VEGF), and the activation of the VEGF pathway is sufficient to suppress the gridlock phenotype...
  59. Yousfi N, Pruvot B, Lopez T, Magadoux L, Franche N, Pichon L, et al. The impact of tumor nitric oxide production on VEGFA expression and tumor growth in a zebrafish rat glioma xenograft model. PLoS ONE. 2015;10:e0120435 pubmed publisher
    ..Furthermore, we demonstrated by qRT-PCR that the transplanted glioma cells highly expressed Nos2, Vegfa and Cyclin D1 mRNA. In the xenografted embryos we also found increased zebrafish vegfa expression...
  60. Schultz L, Solin S, Wierson W, Lovan J, Syrkin Nikolau J, Lincow D, et al. Vascular Endothelial Growth Factor A and Leptin Expression Associated with Ectopic Proliferation and Retinal Dysplasia in Zebrafish Optic Pathway Tumors. Zebrafish. 2017;14:343-356 pubmed publisher
    ..Hif-? targets VEGFA (vegfab) and Leptin (lepb) were both highly upregulated in dysplastic retina; however, each showed distinct ..
  61. Kuroyanagi J, Shimada Y, Zhang B, Ariyoshi M, Umemoto N, Nishimura Y, et al. Zinc finger MYND-type containing 8 promotes tumour angiogenesis via induction of vascular endothelial growth factor-A expression. FEBS Lett. 2014;588:3409-16 pubmed publisher
    ..Notably, ZMYND8 induced vegfa mRNA expression selectively in prostate cancer xenografts...
  62. Matsuoka R, Marass M, Avdesh A, Helker C, Maischein H, Grosse A, et al. Radial glia regulate vascular patterning around the developing spinal cord. elife. 2016;5: pubmed publisher
    ..Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae,..
  63. Priyadarshini M, Tuimala J, Chen Y, Panula P. A zebrafish model of PINK1 deficiency reveals key pathway dysfunction including HIF signaling. Neurobiol Dis. 2013;54:127-38 pubmed publisher
    ..Our findings suggest that a lack of pink1 in zebrafish alters many vital and critical pathways in addition to the HIF signaling pathway. ..
  64. Liu X, Cai X, Hu B, Mei Z, Zhang D, Ouyang G, et al. Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL). J Biol Chem. 2016;291:25692-25705 pubmed
    ..In conclusion, our findings suggest that, by modulating hypoxia-inducible factor activity via up-regulation of VHL, FOXO3a (foxo3b) plays an important role in survival in response to hypoxic stress. ..
  65. Jörgens K, Stoll S, Pohl J, Fleming T, Sticht C, Nawroth P, et al. High tissue glucose alters intersomitic blood vessels in zebrafish via methylglyoxal targeting the VEGF receptor signaling cascade. Diabetes. 2015;64:213-25 pubmed publisher
    ..MG enhanced phosphorylation of vascular endothelial growth factor (VEGF) receptor 2 and its downstream target Akt/protein kinase B (PKB)...
  66. Kubota Y, Oike Y, Satoh S, Tabata Y, Niikura Y, Morisada T, et al. Cooperative interaction of Angiopoietin-like proteins 1 and 2 in zebrafish vascular development. Proc Natl Acad Sci U S A. 2005;102:13502-7 pubmed
    ..These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities. ..
  67. Li S, Zhou X, Dang Y, Kwan Y, Chan S, Leung G, et al. Basal Flt1 tyrosine kinase activity is a positive regulator of endothelial survival and vascularization during zebrafish embryogenesis. Biochim Biophys Acta. 2015;1850:373-84 pubmed publisher
    ..but the role of Flt1 (VEGFR-1) is not well studied and is generally considered as a decoy receptor for trapping VEGF. The effects of VEGFR1/2 kinase inhibitor (VRI) and calycosin on Flt1 tyrosine kinase (TK) activity were evaluated ..
  68. Carroll K, Esain V, Garnaas M, Cortes M, Dovey M, Nissim S, et al. Estrogen defines the dorsal-ventral limit of VEGF regulation to specify the location of the hemogenic endothelial niche. Dev Cell. 2014;29:437-53 pubmed publisher
    ..flk1+ vessel maturation, ephrinB2+ arterial identity, and specification of scl+ HE by decreasing expression of VEGFAa and downstream arterial Notch-pathway components; heat shock induction of VEGFAa/Notch rescued E2-mediated ..
  69. Lima A, Cha B, Amin J, Smith L, Anderson B. Zebrafish embryo model of Bartonella henselae infection. Zebrafish. 2014;11:434-46 pubmed publisher
    ..Thus, the zebrafish embryo provides the first practical model of Bh infection that will facilitate efforts to identify virulence factors and define molecular mechanisms of Bh pathogenesis. ..
  70. Chun C, Chen T. Microinjecting recombinant rainbow trout Ea4-peptide of pro-IGF-I into zebrafish embryos causes abnormal development in heart, red blood cells, and vasculature. Comp Biochem Physiol C Toxicol Pharmacol. 2007;145:39-44 pubmed
    ..Furthermore, the mRNA levels of Nkx2.5, GATA5, VEGF, GATA1 and GATA2 genes in defective embryos were significantly reduced by rtEa4-peptide...
  71. Tu H, Lee G, Hsiao T, Kao T, Wang T, Tsai J, et al. One crisis, diverse impacts-Tissue-specificity of folate deficiency-induced circulation defects in zebrafish larvae. PLoS ONE. 2017;12:e0188585 pubmed publisher
    ..This study also supports the use of this model for further research on the defective cardiogenesis and hematopoiesis caused by folate deficiency. ..
  72. Ober E, Olofsson B, Makinen T, Jin S, Shoji W, Koh G, et al. Vegfc is required for vascular development and endoderm morphogenesis in zebrafish. EMBO Rep. 2004;5:78-84 pubmed
  73. Shivanna S, Harrold I, Shashar M, Meyer R, Kiang C, Francis J, et al. The c-Cbl ubiquitin ligase regulates nuclear β-catenin and angiogenesis by its tyrosine phosphorylation mediated through the Wnt signaling pathway. J Biol Chem. 2015;290:12537-46 pubmed publisher
    ..c-Cbl activation also inhibits expression of the pro-angiogenic Wnt targets, IL-8 and VEGF. Phospho-Tyr-731-inactive mutant c-Cbl (Y731F) enhances and phosphomimetic mutant c-Cbl (Y731E) suppresses ..
  74. Jensen L, Nakamura M, Bräutigam L, Li X, Liu Y, Samani N, et al. VEGF-B-Neuropilin-1 signaling is spatiotemporally indispensable for vascular and neuronal development in zebrafish. Proc Natl Acad Sci U S A. 2015;112:E5944-53 pubmed publisher
    ..Consistent with phenotypic defects, Vegfba, but not Vegfaa, mRNA was primarily expressed in the brain of developing zebrafish embryos...
  75. He M, Liu L, Ge W, Shaw P, Jiang R, Wu L, et al. Antiangiogenic activity of Tripterygium wilfordii and its terpenoids. J Ethnopharmacol. 2009;121:61-8 pubmed publisher
    ..Tripterygium wilfordii Hook. f. (Celastraceae) has been traditionally used as folk medicine for centuries in China for the treatment of immune-inflammatory diseases...
  76. Jin D, Zhu D, Fang Y, Chen Y, Yu G, Pan W, et al. Vegfa signaling regulates diverse artery/vein formation in vertebrate vasculatures. J Genet Genomics. 2017;44:483-492 pubmed publisher
    b>Vascular endothelial growth factor A (Vegfa) signaling regulates vascular development during embryogenesis and organ formation...
  77. Nagasawa Masuda A, Terai K. ERK activation in endothelial cells is a novel marker during neovasculogenesis. Genes Cells. 2016;21:1164-1175 pubmed publisher
    ..Our data show that the ERK activity is a novel marker for determining the emergence of cells in both angiogenesis and lymphangiogenesis. ..
  78. Jiang L, Yin M, Xu J, Jia M, Sun S, Wang X, et al. The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish. Oxid Med Cell Longev. 2017;2017:2143875 pubmed publisher
    ..and suppressed endogenous Wnt/β-catenin signaling and Wnt8a stimulated vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) gene expression at early embryonic stages of zebrafish...
  79. Ernens I, Lumley A, Zhang L, Devaux Y, Wagner D. Hypoxia inhibits lymphatic thoracic duct formation in zebrafish. Biochem Biophys Res Commun. 2017;482:1129-1134 pubmed publisher
    ..While vascular endothelial growth factor A (VEGFA) is critical for angiogenesis, VEGFC and its receptor VEGF receptor-3 (VEGFR-3) are ..
  80. Kawamura H, Li X, Goishi K, van Meeteren L, Jakobsson L, Cebe Suarez S, et al. Neuropilin-1 in regulation of VEGF-induced activation of p38MAPK and endothelial cell organization. Blood. 2008;112:3638-49 pubmed publisher
    Vascular endothelial growth factor (VEGF)-A regulates vascular development and angiogenesis. VEGF isoforms differ in ability to bind coreceptors heparan sulfate (HS) and neuropilin-1 (NRP1)...
  81. Lamont R, Vu W, Carter A, Serluca F, Macrae C, Childs S. Hedgehog signaling via angiopoietin1 is required for developmental vascular stability. Mech Dev. 2010;127:159-68 pubmed publisher
    ..This is the first direct in vivo link between hedgehog signaling and the induction of vascular stability by recruitment of perivascular mural cells through angiopoietin signaling. ..
  82. Feldner J, Becker T, Goishi K, Schweitzer J, Lee P, Schachner M, et al. Neuropilin-1a is involved in trunk motor axon outgrowth in embryonic zebrafish. Dev Dyn. 2005;234:535-49 pubmed
    Neuropilin-1, a receptor for axon-repellent semaphorins and vascular endothelial growth factor (VEGF), functions both in angiogenesis and axon growth...
  83. Rowlinson J, Gering M. Hey2 acts upstream of Notch in hematopoietic stem cell specification in zebrafish embryos. Blood. 2010;116:2046-56 pubmed publisher
    ..These results establish an essential role for Hey2 upstream of Notch in HSC formation. ..
  84. Corallo D, Schiavinato A, Trapani V, Moro E, Argenton F, Bonaldo P. Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development. 2013;140:4594-601 pubmed publisher
    ..Overall, this study reveals a new role for an EMILIN protein and reinforces the concept that structure and function of the notochord are strictly linked. ..