spaw

Summary

Gene Symbol: spaw
Description: southpaw
Alias: southpaw, ndr3, unm s457, unm t30973, unm_s457, unm_t30973
Species: zebrafish

Top Publications

  1. Saude L, Lourenço R, Gonçalves A, Palmeirim I. terra is a left-right asymmetry gene required for left-right synchronization of the segmentation clock. Nat Cell Biol. 2005;7:918-20 pubmed
    ..Here, we show that terra is an early left-sided expressed gene that links left-right patterning with bilateral synchronization of the segmentation clock. ..
  2. Hong S, Dawid I. FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1. Proc Natl Acad Sci U S A. 2009;106:2230-5 pubmed publisher
    ..We conclude that Ier2 and Fibp1 mediate FGF signaling in ciliogenesis in Kupffer's Vesicle and in the establishment of laterality in the zebrafish embryo. ..
  3. Serluca F, Xu B, Okabe N, Baker K, Lin S, Sullivan Brown J, et al. Mutations in zebrafish leucine-rich repeat-containing six-like affect cilia motility and result in pronephric cysts, but have variable effects on left-right patterning. Development. 2009;136:1621-31 pubmed publisher
    ..Combined with recently published results, our alleles suggest that the function of seahorse in cilia motility is separable from its function in other cilia-related phenotypes. ..
  4. Zhai G, Gu Q, He J, Lou Q, Chen X, Jin X, et al. Sept6 is required for ciliogenesis in Kupffer's vesicle, the pronephros, and the neural tube during early embryonic development. Mol Cell Biol. 2014;34:1310-21 pubmed publisher
    ..Consequently, cilium-related functions, such as the left-right patterning of internal organs and nodal/spaw signaling, are compromised...
  5. Tay H, Schulze S, Compagnon J, Foley F, Heisenberg C, Yost H, et al. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle. Development. 2013;140:1550-9 pubmed publisher
    ..These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish. ..
  6. Neugebauer J, Amack J, Peterson A, Bisgrove B, Yost H. FGF signalling during embryo development regulates cilia length in diverse epithelia. Nature. 2009;458:651-4 pubmed publisher
    ..We propose that a subset of developmental defects and diseases ascribed to FGF signalling are due in part to loss of cilia function. ..
  7. Sarmah B, Latimer A, Appel B, Wente S. Inositol polyphosphates regulate zebrafish left-right asymmetry. Dev Cell. 2005;9:133-45 pubmed
    ..Our data suggest that the pathway for inositol hexakisphosphate production is a key regulator of asymmetric Ca(2+) flux during LR specification. ..
  8. Schottenfeld J, Sullivan Brown J, Burdine R. Zebrafish curly up encodes a Pkd2 ortholog that restricts left-side-specific expression of southpaw. Development. 2007;134:1605-15 pubmed
    ..In the majority of cup embryos, the zebrafish nodal gene southpaw (spaw) is activated bilaterally in LPM, as opposed to the complete absence of Nodal reported in the LPM of the ..
  9. Oteiza P, Köppen M, Krieg M, Pulgar E, Farias C, Melo C, et al. Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ. Development. 2010;137:3459-68 pubmed publisher
    ..Our results reveal a novel role for PCP-dependent cell adhesion in coordinating the supracellular organisation of progenitor cells during vertebrate laterality organ formation. ..

More Information

Publications62

  1. Monteiro R, Van Dinther M, Bakkers J, Wilkinson R, Patient R, Ten Dijke P, et al. Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish. Dev Biol. 2008;315:55-71 pubmed publisher
    ..laterality markers in morphants indicated that bmpr2a and bmpr2b act upstream of pitx2 and the nodal-related southpaw (spaw), which are expressed asymmetrically in the lateral plate mesoderm (LPM), and subsequently regulate lefty2 ..
  2. Gao C, Wang G, Amack J, Mitchell D. Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis. Dev Dyn. 2010;239:2190-7 pubmed publisher
    ..Analysis of cilium ultrastructure revealed loss of outer dynein arms in morphant embryos. These results support a remarkable level of functional conservation for Oda16/Wdr69. ..
  3. Caron A, Xu X, Lin X. Wnt/?-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle. Development. 2012;139:514-24 pubmed publisher
    ..Moreover, our results also prompt a hypothesis that certain developmental effects of the Wnt/?-catenin pathway are due to the activation of Foxj1 and cilia formation...
  4. Ablooglu A, Tkachenko E, Kang J, Shattil S. Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry. Development. 2010;137:3449-58 pubmed publisher
  5. Carl M, Bianco I, Bajoghli B, Aghaallaei N, Czerny T, Wilson S. Wnt/Axin1/beta-catenin signaling regulates asymmetric nodal activation, elaboration, and concordance of CNS asymmetries. Neuron. 2007;55:393-405 pubmed
    ..We identify a second role for the Wnt pathway in the left/right regulation of LPM Nodal pathway gene expression, and finally, we show that at later stages Axin1 is required for the elaboration of concordant neuroanatomical asymmetries. ..
  6. Soroldoni D, Bajoghli B, Aghaallaei N, Czerny T. Dynamic expression pattern of Nodal-related genes during left-right development in medaka. Gene Expr Patterns. 2007;7:93-101 pubmed publisher
    ..Based on sequence analysis and in accordance to zebrafish orthologues we named the isolated genes Ndr1, Ndr2 and Spaw. Gene expression analysis throughout medaka development confirmed this assignment...
  7. Zhang M, Zhang J, Lin S, Meng A. ?-Catenin 1 and ?-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos. Development. 2012;139:2009-19 pubmed publisher
    ..ctnnb1 and ctnnb2 in DFCs/KV differentially regulate the expression of charon, a Nodal antagonist, and spaw, a key Nodal gene for laterality development in zebrafish...
  8. Kramer Zucker A, Olale F, Haycraft C, Yoder B, Schier A, Drummond I. Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis. Development. 2005;132:1907-21 pubmed
    ..In Kupffer's vesicle, loss of flow is associated with loss of left-right patterning, indicating that the 'nodal flow' mechanism of generating situs is conserved in non-mammalian vertebrates. ..
  9. Burckle C, Gaudé H, Vesque C, Silbermann F, Salomon R, Jeanpierre C, et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet. 2011;20:2611-27 pubmed publisher
    ..Moreover, they highlight common signalling functions for inversin and nephrocystin-4, suggesting that these two nephrocystins are involved in common physiopathological mechanisms. ..
  10. Wang X, Yost H. Initiation and propagation of posterior to anterior (PA) waves in zebrafish left-right development. Dev Dyn. 2008;237:3640-7 pubmed publisher
    ..Here, we quantitatively describe the timing of the posterior to anterior (PA) wave-like propagation of zebrafish southpaw (Nodal) and pitx2 in LPM and lefty1 in the midline...
  11. Long S, Ahmad N, Rebagliati M. The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry. Development. 2003;130:2303-16 pubmed
    We have identified and characterized a new zebrafish gene, southpaw, that is required for visceral and diencephalic left-right asymmetry...
  12. Lopes S, Lourenço R, Pacheco L, Moreno N, Kreiling J, Saude L. Notch signalling regulates left-right asymmetry through ciliary length control. Development. 2010;137:3625-32 pubmed publisher
    ..Notch signalling brings together ciliary length control and fluid flow hydrodynamics with transcriptional activation of laterality genes. In addition, our deltaD mutant analysis discloses an uncoupling between gut and heart laterality. ..
  13. Albertson R, Yelick P. Roles for fgf8 signaling in left-right patterning of the visceral organs and craniofacial skeleton. Dev Biol. 2005;283:310-21 pubmed
    ..These data provide new insights into vertebrate laterality and offer the zebrafish ace/fgf8 mutant as a novel molecular tool to investigate tissue-specific molecular laterality mechanisms. ..
  14. Fogelgren B, Lin S, Zuo X, Jaffe K, Park K, Reichert R, et al. The exocyst protein Sec10 interacts with Polycystin-2 and knockdown causes PKD-phenotypes. PLoS Genet. 2011;7:e1001361 pubmed publisher
    ..Our work supports a model in which the exocyst is required for the ciliary localization of polycystin-2, thus allowing for polycystin-2 function in cellular processes. ..
  15. May Simera H, Kai M, Hernandez V, Osborn D, Tada M, Beales P. Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish. Dev Biol. 2010;345:215-25 pubmed publisher
    ..These data suggest that bbs8 and vangl2 act synergistically on cell polarization to establish and maintain the appropriate length and number of cilia in the KV and thereby facilitate correct LR asymmetry. ..
  16. Duldulao N, Lee S, Sun Z. Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion. Development. 2009;136:4033-42 pubmed publisher
    ..Together, these results strongly support the hypothesis that JS-related disease (JSRD) is a ciliopathy, or a disease caused by ciliary defects, and that Arl13b functions mainly through the cilium. ..
  17. Chocron S, Verhoeven M, Rentzsch F, Hammerschmidt M, Bakkers J. Zebrafish Bmp4 regulates left-right asymmetry at two distinct developmental time points. Dev Biol. 2007;305:577-88 pubmed
    ..express either noggin3 or bmp2b, we show a requirement for BMP signaling during early segmentation to repress southpaw expression in the right lateral plate mesoderm and regulate both visceral and heart laterality...
  18. Adams D, Robinson K, Fukumoto T, Yuan S, Albertson R, Yelick P, et al. Early, H+-V-ATPase-dependent proton flux is necessary for consistent left-right patterning of non-mammalian vertebrates. Development. 2006;133:1657-71 pubmed
    ..both species; in chick, H+-V-ATPase activity is upstream of Shh; in fish, it is upstream of Kupffer's vesicle and Spaw expression...
  19. Yamauchi H, Miyakawa N, Miyake A, Itoh N. Fgf4 is required for left-right patterning of visceral organs in zebrafish. Dev Biol. 2009;332:177-85 pubmed publisher
    ..Lefty1, lefty2, southpaw, and pitx2 are known to play crucial roles in LR patterning of visceral organs...
  20. Roussigné M, Bianco I, Wilson S, Blader P. Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development. 2009;136:1549-57 pubmed publisher
    ..Our results provide evidence of a direct requirement for unilateral Nodal activity in establishing an asymmetry per se, rather than solely in biasing its laterality. ..
  21. Aamar E, Dawid I. Sox17 and chordin are required for formation of Kupffer's vesicle and left-right asymmetry determination in zebrafish. Dev Dyn. 2010;239:2980-8 pubmed publisher
    ..Sox17 and Chd in DFC led to aberrant Left-Right (L-R) asymmetry establishment, as visualized by the expression of southpaw and lefty, and heart and pancreas placement in the embryo...
  22. Yu X, Ng C, Habacher H, Roy S. Foxj1 transcription factors are master regulators of the motile ciliogenic program. Nat Genet. 2008;40:1445-53 pubmed publisher
    ..Our findings identify a dedicated master regulatory role for Foxj1 in the transcriptional program that controls the production of motile cilia. ..
  23. van Rooijen E, Giles R, Voest E, van Rooijen C, Schulte Merker S, van Eeden F. LRRC50, a conserved ciliary protein implicated in polycystic kidney disease. J Am Soc Nephrol. 2008;19:1128-38 pubmed publisher
  24. Smith K, Chocron S, von der Hardt S, de Pater E, Soufan A, Bussmann J, et al. Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells. Dev Cell. 2008;14:287-97 pubmed publisher
    ..Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart. ..
  25. Schneider I, Houston D, Rebagliati M, Slusarski D. Calcium fluxes in dorsal forerunner cells antagonize beta-catenin and alter left-right patterning. Development. 2008;135:75-84 pubmed
    ..As in zebrafish, manipulation of Ca(2+) release results in ectopic nuclear beta-catenin and altered laterality. Overall, our data support a conserved early Ca(2+) requirement in DFC-like cell function in zebrafish and Xenopus. ..
  26. Pathak N, Obara T, Mangos S, Liu Y, Drummond I. The zebrafish fleer gene encodes an essential regulator of cilia tubulin polyglutamylation. Mol Biol Cell. 2007;18:4353-64 pubmed
    ..These results are the first in vivo evidence that tubulin polyglutamylation is required for vertebrate cilia motility and structure, and, when compromised, results in failed ciliogenesis. ..
  27. Tian T, Zhao L, Zhang M, Zhao X, Meng A. Both foxj1a and foxj1b are implicated in left-right asymmetric development in zebrafish embryos. Biochem Biophys Res Commun. 2009;380:537-42 pubmed publisher
    ..at the one-cell stage results in randomized expression of the early left-right (LR) asymmetric markers lefty2, southpaw, pitx2c and the later internal organ markers tpm4-tv1, cmlc2, cp in zebrafish embryos...
  28. Matsui T, Thitamadee S, Murata T, Kakinuma H, Nabetani T, Hirabayashi Y, et al. Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis. Proc Natl Acad Sci U S A. 2011;108:9881-6 pubmed publisher
  29. Kishimoto N, Cao Y, Park A, Sun Z. Cystic kidney gene seahorse regulates cilia-mediated processes and Wnt pathways. Dev Cell. 2008;14:954-61 pubmed publisher
    ..Finally, we show that seahorse constrains the canonical Wnt pathway and promotes the noncanonical Wnt pathway during gastrulation. Together, these data suggest that Seahorse may provide a link between ciliary signals and Wnt pathways. ..
  30. Shu X, Huang J, Dong Y, Choi J, Langenbacher A, Chen J. Na,K-ATPase alpha2 and Ncx4a regulate zebrafish left-right patterning. Development. 2007;134:1921-30 pubmed
  31. Bisgrove B, Snarr B, Emrazian A, Yost H. Polaris and Polycystin-2 in dorsal forerunner cells and Kupffer's vesicle are required for specification of the zebrafish left-right axis. Dev Biol. 2005;287:274-88 pubmed
    ..knockdown of Polaris or Pkd2 in zebrafish results in misexpression of left-side-specific genes, including southpaw, lefty1 and lefty2, and randomization of heart and gut looping...
  32. Ahmad N, Long S, Rebagliati M. A southpaw joins the roster: the role of the zebrafish nodal-related gene southpaw in cardiac LR asymmetry. Trends Cardiovasc Med. 2004;14:43-9 pubmed
    ..The nodal-related gene southpaw is required for both aspects of heart LR asymmetry as well as for LR patterning of other visceral organs...
  33. Hashimoto H, Rebagliati M, Ahmad N, Muraoka O, Kurokawa T, Hibi M, et al. The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish. Development. 2004;131:1741-53 pubmed publisher
    ..the dorsalizing activity of all three of the known zebrafish Nodal-related proteins (Cyclops, Squint and Southpaw), indicating that Charon can antagonize Nodal signaling...
  34. Amack J, Wang X, Yost H. Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. Dev Biol. 2007;310:196-210 pubmed
    ..By targeting morpholinos to DFCs, we show that these cell autonomous functions in KV morphogenesis are necessary for LR patterning throughout the embryo. ..
  35. Gamse J, Kuan Y, Macurak M, Brösamle C, Thisse B, Thisse C, et al. Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target. Development. 2005;132:4869-81 pubmed
    ..The results demonstrate that laterality of the dorsal forebrain influences the formation of midbrain connections and their molecular properties. ..
  36. Francescatto L, Rothschild S, Myers A, Tombes R. The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry. Development. 2010;137:2753-62 pubmed publisher
    ..morpholino oligonucleotides, as well as KV-targeted dominant negative CaMK-II, randomize organ laterality and southpaw (spaw) expression in lateral plate mesoderm (LPM)...
  37. Gao W, Xu L, Guan R, Liu X, Han Y, Wu Q, et al. Wdr18 is required for Kupffer's vesicle formation and regulation of body asymmetry in zebrafish. PLoS ONE. 2011;6:e23386 pubmed publisher
    ..defects in the visceral organs, which were preceded by the mis-expression of Nodal-related genes, including spaw and pitx2...
  38. John L, Trengove M, Fraser F, Yoong S, Ward A. Pegasus, the 'atypical' Ikaros family member, influences left-right asymmetry and regulates pitx2 expression. Dev Biol. 2013;377:46-54 pubmed publisher
    ..affected both the level and asymmetry of pitx2 expression, as well as disrupting the asymmetry of the lefty2 and spaw genes, explaining the perturbed left-right patterning in pegasus morphants...
  39. Zhao X, Jin M, Wang M, Sun L, Hong X, Cao Y, et al. Fidgetin-like 1 is a ciliogenesis-inhibitory centrosome protein. Cell Cycle. 2016;15:2367-75 pubmed publisher
    ..These results extend the already long list of centrosomal proteins and provide new insights into the regulation of ciliogenesis. ..
  40. Stiff T, Casar Tena T, O Driscoll M, Jeggo P, Philipp M. ATR promotes cilia signalling: links to developmental impacts. Hum Mol Genet. 2016;25:1574-87 pubmed publisher
    ..Additionally, they displayed defects in left-right asymmetry including ambiguous expression of southpaw, incorrectly looped hearts and randomized localization of internal organs including the pancreas, features ..
  41. Sampaio P, Ferreira R, Guerrero A, Pintado P, Tavares B, Amaro J, et al. Left-right organizer flow dynamics: how much cilia activity reliably yields laterality?. Dev Cell. 2014;29:716-28 pubmed publisher
    ..We conclude that cilia number, dorsal anterior motile cilia clustering, and left flow are critical to situs solitus via robust asymmetric charon expression. ..
  42. Niederriter A, Davis E, Golzio C, Oh E, Tsai I, Katsanis N. In vivo modeling of the morbid human genome using Danio rerio. J Vis Exp. 2013;:e50338 pubmed publisher
    ..We present our established models for morphogenetic signaling, craniofacial, cardiac, vascular integrity, renal function, and skeletal muscle disorder phenotypes, as well as others. ..
  43. Burkhalter M, Fralish G, Premont R, Caron M, Philipp M. Grk5l controls heart development by limiting mTOR signaling during symmetry breaking. Cell Rep. 2013;4:625-32 pubmed publisher
    ..These findings could implicate GRK5 as a susceptibility allele for certain cases of CHD. ..
  44. Hochgreb Hägele T, Yin C, Koo D, Bronner M, Stainier D. Laminin ?1a controls distinct steps during the establishment of digestive organ laterality. Development. 2013;140:2734-45 pubmed publisher
    ..Loss of Lamb1a function also results in aberrant protrusion of LPM cells into the gut. Collectively, our results provide cellular and molecular mechanisms by which extracellular matrix proteins regulate left-right organ morphogenesis. ..
  45. Kawakami Y, Raya A, Raya R, Rodriguez Esteban C, Izpisua Belmonte J. Retinoic acid signalling links left-right asymmetric patterning and bilaterally symmetric somitogenesis in the zebrafish embryo. Nature. 2005;435:165-71 pubmed
  46. Smith K, Noël E, Thurlings I, Rehmann H, Chocron S, Bakkers J. Bmp and nodal independently regulate lefty1 expression to maintain unilateral nodal activity during left-right axis specification in zebrafish. PLoS Genet. 2011;7:e1002289 pubmed publisher
    ..In Bmpr1a-deficient embryos we observed bilateral expression of the Nodal-related gene, spaw, coupled with reduced expression of the Nodal-antagonist lefty1 in the midline...
  47. Wang L, Liu Z, Lin H, Ma D, Tao Q, Liu F. Epigenetic regulation of left-right asymmetry by DNA methylation. EMBO J. 2017;36:2987-2997 pubmed publisher
    ..1 regulates collective DFC migration through cadherin 1 (Cdh1). Taken together, our data uncover dynamic DNA methylation as an epigenetic mechanism to control LR determination during early embryogenesis in vertebrates. ..
  48. Frisca F, Colquhoun D, Goldshmit Y, Ankö M, Pebay A, Kaslin J. Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish. Sci Rep. 2016;6:37678 pubmed publisher
    ..Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis. ..
  49. Peterson A, Wang X, Yost H. Dvr1 transfers left-right asymmetric signals from Kupffer's vesicle to lateral plate mesoderm in zebrafish. Dev Biol. 2013;382:198-208 pubmed publisher
    ..Knockdown of Dvr1 by morpholino causes dramatically reduced or absent expression of southpaw (spaw, a Nodal homolog), in LPM, and corresponding loss of downstream Lefty (lft1 and lft) expression, and ..
  50. Tessadori F, Noël E, Rens E, Magliozzi R, Evers van Gogh I, Guardavaccaro D, et al. Nodal signaling range is regulated by proprotein convertase-mediated maturation. Dev Cell. 2015;32:631-9 pubmed publisher
    ..Embryological and biochemical experiments demonstrate that cleavage of the Nodal-related Spaw proprotein into a mature form by FurinA is required for Spaw gradient formation and activation of Nodal signaling...
  51. Tarkar A, Loges N, Slagle C, Francis R, Dougherty G, Tamayo J, et al. DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nat Genet. 2013;45:995-1003 pubmed publisher
    ..Thus, we propose that DYX1C1 is a newly identified dynein axonemal assembly factor (DNAAF4)...
  52. Leung T, Humbert J, Stauffer A, Giger K, Chen H, Tsai H, et al. The orphan G protein-coupled receptor 161 is required for left-right patterning. Dev Biol. 2008;323:31-40 pubmed publisher
    ..Taken together, these data support a model in which gpr161 plays an essential role in left-right (L-R) patterning by modulating Ca(2+) levels in the cells surrounding the Kupffer's vesicle. ..
  53. Tian T, Zhao L, Zhao X, Zhang M, Meng A. A zebrafish gene trap line expresses GFP recapturing expression pattern of foxj1b. J Genet Genomics. 2009;36:581-9 pubmed publisher
    ..Nevertheless, this transgenic line may serve as a useful model for dynamic observation of GFP-labeled tissues and organs and for isolation of GFP-labeled cells. ..