Gene Symbol: smad4a
Description: SMAD family member 4a
Alias: madh4, smad4, zmp:0000000768, mothers against decapentaplegic homolog 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, MAD homolog 4, SMA- and MAD-related protein 4, SMAD family member 4
Species: zebrafish
Products:     smad4a

Top Publications

  1. Shen L, Chen F, Zhang Y, Cao L, Kuang Y, Zhong M, et al. MYCN transgenic zebrafish model with the characterization of acute myeloid leukemia and altered hematopoiesis. PLoS ONE. 2013;8:e59070 pubmed publisher
    ..Therefore, this MYCN transgenic zebrafish model facilitates dissection of MYCN-mediated signaling in vivo, and enables high-throughput scale screens to identify the potential therapeutic targets. ..
  2. Sun H, Li D, Chen S, Liu Y, Liao X, Deng W, et al. Zili inhibits transforming growth factor-beta signaling by interacting with Smad4. J Biol Chem. 2010;285:4243-50 pubmed publisher
    ..The results of protein interaction assays identify that Zili binds to Smad4 via its N-terminal domain and prevents the formation of Smad2/3/4 and Smad1/5/9/4 complexes to antagonize TGF-beta ..
  3. Wei C, Wang H, Zhu Z, Sun Y. Transcriptional factors smad1 and smad9 act redundantly to mediate zebrafish ventral specification downstream of smad5. J Biol Chem. 2014;289:6604-18 pubmed publisher
    ..In conclusion, our study uncovers that smad1 and smad9 act redundantly to each other downstream of smad5 to mediate ventral specification and to regulate embryonic myelopoiesis. ..
  4. Hsu R, Lin C, Su Y, Tsai H. dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity. J Biol Chem. 2011;286:6855-64 pubmed publisher
    ..These results support the involvement of Smad2·Smad3a in p38a mediation. Overexpression of Smad4 enabled the rescue of myf5 defects in the dkk3r-MO-injected embryos, but knockdown of either dkk3r or p38a caused ..
  5. Sun Y, Tseng W, Fan X, Ball R, Dougan S. Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning. Dev Cell. 2014;28:322-34 pubmed publisher
    ..Swr) expressed under the control of Max's giant associated protein (MGA) and its binding partners, Max and Smad4. MGA coimmunoprecipitates with both Max and Smad4 in embryo extracts, and the three proteins form a complex in ..
  6. Shen L, Zhu J, Chen F, Lin W, Cai J, Zhong J, et al. RUNX1-Evi-1 fusion gene inhibited differentiation and apoptosis in myelopoiesis: an in vivo study. BMC Cancer. 2015;15:970 pubmed publisher
    ..VPA may be a promising choice in the molecular targeting therapy of RUNX1-Evi-1-related leukemia. ..
  7. Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, et al. USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling. EMBO J. 2017;36:1623-1639 pubmed publisher
    b>SMAD4 is a common intracellular effector for TGF-? family cytokines, but the mechanism by which its activity is dynamically regulated is unclear...
  8. Harvey S, Smith J. Visualisation and quantification of morphogen gradient formation in the zebrafish. PLoS Biol. 2009;7:e1000101 pubmed publisher
    ..we have used bimolecular fluorescence complementation to visualise the formation of a complex between Smad2 and Smad4. This has allowed us to visualise, in living embryos, the formation of a graded distribution of nodal signalling ..
  9. Casari A, Schiavone M, Facchinello N, Vettori A, Meyer D, Tiso N, et al. A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Dev Biol. 2014;396:81-93 pubmed publisher

More Information


  1. Yang J, Wang J, Zeng Z, Qiao L, Zhuang L, Jiang L, et al. Smad4 is required for the development of cardiac and skeletal muscle in zebrafish. Differentiation. 2016;92:161-168 pubmed publisher
    ..b>Smad4 is the central intracellular mediator of TGF-beta signaling and plays crucial roles in tissue regeneration, cell ..
  2. Dick A, Mayr T, Bauer H, Meier A, Hammerschmidt M. Cloning and characterization of zebrafish smad2, smad3 and smad4. Gene. 2000;246:69-80 pubmed
    ..We report here the cloning, characterization and genomic mapping of smad2, smad3 and smad4 from the zebrafish, Danio rerio...
  3. Bian S, Zheng X, Sun H, Chen J, Lu Y, Liu Y, et al. Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo. J Biol Chem. 2017;292:14165-14175 pubmed publisher
  4. Liu J, Xu Q, Li S, Yu X, Liu W, Ouyang G, et al. Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-? signaling. Biochim Biophys Acta Gene Regul Mech. 2017;1860:1103-1116 pubmed publisher
  5. Hozumi S, Aoki S, Kikuchi Y. Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification. Development. 2017;144:4015-4025 pubmed publisher
    ..Our study provides insight into the function of nuclear movement in Smad signaling toward the nucleus, and could be applied to the control of TGF? signaling. ..