Gene Symbol: smad3a
Description: SMAD family member 3a
Alias: madh3, madh3a, smad3, wu:fa99e03, SMAD family member 3a, MAD homolog 3, MAD homolog 3a
Species: zebrafish
Products:     smad3a

Top Publications

  1. Jia S, Wu D, Xing C, Meng A. Smad2/3 activities are required for induction and patterning of the neuroectoderm in zebrafish. Dev Biol. 2009;333:273-84 pubmed publisher
    Smad2 and Smad3, two essential nuclear effectors of transforming growth factor (Tgf)-beta signals, have been found to be implicated in mesoderm and endoderm development in vertebrate embryos...
  2. Jia S, Ren Z, Li X, Zheng Y, Meng A. smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/nodal signals in zebrafish. J Biol Chem. 2008;283:2418-26 pubmed
    ..We generated potent and specific dominant-negative forms of zebrafish Smad2, Smad3a, and Smad3b by mutating multiple amino acids...
  3. Casari A, Schiavone M, Facchinello N, Vettori A, Meyer D, Tiso N, et al. A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Dev Biol. 2014;396:81-93 pubmed publisher
    ..Reporter fluorescence is activated in phospho-Smad3 positive cells and is responsive to both Smad3 isoforms, Smad3a and 3b. Moreover, Alk4 and Alk5 inhibitors strongly repress the reporter activity...
  4. Tan Q, Balofsky A, Weisz K, Peng C. Role of activin, transforming growth factor-beta and bone morphogenetic protein 15 in regulating zebrafish oocyte maturation. Comp Biochem Physiol A Mol Integr Physiol. 2009;153:18-23 pubmed publisher
    ..These findings suggest that activin-A, TGF-beta1, and BMP-15 may target common gene(s) to regulate oocyte maturation and demonstrate that Smad2 plays an important role in oocyte maturation. ..
  5. Liu X, Xiong C, Jia S, Zhang Y, Chen Y, Wang Q, et al. Araf kinase antagonizes Nodal-Smad2 activity in mesendoderm development by directly phosphorylating the Smad2 linker region. Nat Commun. 2013;4:1728 pubmed publisher
    ..Our findings open avenues for investigating the potential significance of Raf regulation of transforming growth factor ? signalling in versatile biological and pathological processes in the future. ..
  6. Bian S, Zheng X, Sun H, Chen J, Lu Y, Liu Y, et al. Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo. J Biol Chem. 2017;292:14165-14175 pubmed publisher
    ..Mechanistically, Clock1a activates the smad3a promoter via its E-box1 element (CAGATG)...
  7. Esguerra C, Nelles L, Vermeire L, Ibrahimi A, Crawford A, Derua R, et al. Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination. Development. 2007;134:4381-93 pubmed
    ..We identified Ttrap as a novel Alk4- and Smad3-interacting protein that controls gastrulation movements and left-right axis determination in zebrafish...
  8. Peng X, Li G, Wang Y, Zhuang J, Luo R, Chen J, et al. CXXC5 is required for cardiac looping relating to TGF? signaling pathway in zebrafish. Int J Cardiol. 2016;214:246-53 pubmed publisher
    ..This finding suggested that CXXC5 may serve as a possible marker that has potential diagnostic and prognostic value in fetus with congenital heart disease. ..
  9. Hsu R, Lin C, Su Y, Tsai H. dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity. J Biol Chem. 2011;286:6855-64 pubmed publisher
    ..Moreover, overexpression of Smad2 or Smad3a enhanced myf5 expression, but the defects induced by the dominant negative form of either Smad2 or Smad3a equaled ..

More Information


  1. Saili K, Tilton S, Waters K, Tanguay R. Global gene expression analysis reveals pathway differences between teratogenic and non-teratogenic exposure concentrations of bisphenol A and 17?-estradiol in embryonic zebrafish. Reprod Toxicol. 2013;38:89-101 pubmed publisher
    ..1?M BPA and 0.1?M E2 exposure, and suppressed expression of several genes involved in nervous system development and function following 0.1?M BPA exposure. ..
  2. Miyares R, Stein C, Renisch B, Anderson J, Hammerschmidt M, Farber S. Long-chain Acyl-CoA synthetase 4A regulates Smad activity and dorsoventral patterning in the zebrafish embryo. Dev Cell. 2013;27:635-47 pubmed publisher
    ..Our results reveal a critical role for Acsl4a in modulating Bmp-Smad activity and provide a potential avenue for LC-PUFAs to influence a variety of developmental processes. ..
  3. Flanagan Steet H, Aarnio M, Kwan B, Guihard P, Petrey A, Haskins M, et al. Cathepsin-Mediated Alterations in TGFß-Related Signaling Underlie Disrupted Cartilage and Bone Maturation Associated With Impaired Lysosomal Targeting. J Bone Miner Res. 2016;31:535-48 pubmed publisher
    ..These findings highlight the complexity of the skeletal disease associated with MLII and bring new insight to the role of secreted cathepsin proteases in cartilage development and growth factor regulation. ..
  4. Samarut E, Bekri A, Drapeau P. Transcriptomic Analysis of Purified Embryonic Neural Stem Cells from Zebrafish Embryos Reveals Signaling Pathways Involved in Glycine-Dependent Neurogenesis. Front Mol Neurosci. 2016;9:22 pubmed publisher
  5. Liu J, Xu Q, Li S, Yu X, Liu W, Ouyang G, et al. Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-? signaling. Biochim Biophys Acta Gene Regul Mech. 2017;1860:1103-1116 pubmed publisher
  6. Sun Y, Tseng W, Fan X, Ball R, Dougan S. Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning. Dev Cell. 2014;28:322-34 pubmed publisher
    ..We conclude that MGA, Max, and Smad4 act in the extraembryonic YSL to initiate a positive feedback loop of Bmp signaling within the embryo. ..
  7. Li Y, Li G, Wang H, Du J, Yan J. Analysis of a gene regulatory cascade mediating circadian rhythm in zebrafish. PLoS Comput Biol. 2013;9:e1002940 pubmed publisher
    ..Our study describes a systematic method to discover previously unidentified TFs involved in circadian physiology in complex organisms. ..