smad2

Summary

Gene Symbol: smad2
Description: SMAD family member 2
Alias: fj43c06, madh2, wu:fj43c06, mothers against decapentaplegic homolog 2, MAD homolog 2, SMA- and MAD-related protein 2, mothers against DPP homolog 2
Species: zebrafish
Products:     smad2

Top Publications

  1. Kiecker C, Muller F, Wu W, Glinka A, Strahle U, Niehrs C. Phenotypic effects in Xenopus and zebrafish suggest that one-eyed pinhead functions as antagonist of BMP signalling. Mech Dev. 2000;94:37-46 pubmed
  2. Zhang Y, Li X, Qi J, Wang J, Liu X, Zhang H, et al. Rock2 controls TGFbeta signaling and inhibits mesoderm induction in zebrafish embryos. J Cell Sci. 2009;122:2197-207 pubmed publisher
    ..Thus, our data have unraveled previously unidentified functions of Rock2, in controlling TGFbeta signaling as well as in regulating embryonic patterning. ..
  3. Jia S, Ren Z, Li X, Zheng Y, Meng A. smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/nodal signals in zebrafish. J Biol Chem. 2008;283:2418-26 pubmed
    ..These ligands are believed to transduce the signal through the receptor-activated transcription factors Smad2 and Smad3...
  4. Gritsman K, Zhang J, Cheng S, Heckscher E, Talbot W, Schier A. The EGF-CFC protein one-eyed pinhead is essential for nodal signaling. Cell. 1999;97:121-32 pubmed
    ..overexpression but are suppressed by expression of Activin and activated forms of the type I receptor ActRIB and Smad2. Expression of the murine EGF-CFC gene cripto rescues oep mutants...
  5. Jia S, Wu D, Xing C, Meng A. Smad2/3 activities are required for induction and patterning of the neuroectoderm in zebrafish. Dev Biol. 2009;333:273-84 pubmed publisher
    b>Smad2 and Smad3, two essential nuclear effectors of transforming growth factor (Tgf)-beta signals, have been found to be implicated in mesoderm and endoderm development in vertebrate embryos...
  6. Hsu R, Lin C, Su Y, Tsai H. dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity. J Biol Chem. 2011;286:6855-64 pubmed publisher
    ..Moreover, overexpression of Smad2 or Smad3a enhanced myf5 expression, but the defects induced by the dominant negative form of either Smad2 or ..
  7. Hozumi S, Aoki S, Kikuchi Y. Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification. Development. 2017;144:4015-4025 pubmed publisher
    ..Our study provides insight into the function of nuclear movement in Smad signaling toward the nucleus, and could be applied to the control of TGF? signaling. ..
  8. Liu J, Xu Q, Li S, Yu X, Liu W, Ouyang G, et al. Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-? signaling. Biochim Biophys Acta Gene Regul Mech. 2017;1860:1103-1116 pubmed publisher
  9. Sun Y, Tseng W, Fan X, Ball R, Dougan S. Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning. Dev Cell. 2014;28:322-34 pubmed publisher
    ..We conclude that MGA, Max, and Smad4 act in the extraembryonic YSL to initiate a positive feedback loop of Bmp signaling within the embryo. ..

More Information

Publications38

  1. Liu X, Xiong C, Jia S, Zhang Y, Chen Y, Wang Q, et al. Araf kinase antagonizes Nodal-Smad2 activity in mesendoderm development by directly phosphorylating the Smad2 linker region. Nat Commun. 2013;4:1728 pubmed publisher
    b>Smad2/3-mediated transforming growth factor ? signalling and the Ras-Raf-Mek-Erk cascade have important roles in stem cell and development and tissue homeostasis...
  2. Wei S, Ning G, Li L, Yan Y, Yang S, Cao Y, et al. A GEF activity-independent function for nuclear Net1 in Nodal signal transduction and mesendoderm formation. J Cell Sci. 2017;130:3072-3082 pubmed publisher
    ..Detailed analyses of protein interactions reveal that Net1 associates with Smad2 in the nucleus in a GEF-independent manner, and then promotes Smad2 activation by enhancing recruitment of p300 (..
  3. Choi J, Dong L, Ahn J, Dao D, Hammerschmidt M, Chen J. FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafish. Dev Biol. 2007;304:735-44 pubmed
    ..of FoxH1 has a negative effect on vascular formation that can be counteracted by the down-regulation of smad2 activity in zebrafish embryos...
  4. Esterberg R, Delalande J, Fritz A. Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm. Development. 2008;135:3891-901 pubmed publisher
    ..Our results illustrate a role for Bmp4 in the proliferation and timely differentiation of axial tissue after DV axis specification. ..
  5. Casari A, Schiavone M, Facchinello N, Vettori A, Meyer D, Tiso N, et al. A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Dev Biol. 2014;396:81-93 pubmed publisher
    ..The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are involved in different pathologies with an ..
  6. Li Villarreal N, Forbes M, Loza A, Chen J, Ma T, Helde K, et al. Dachsous1b cadherin regulates actin and microtubule cytoskeleton during early zebrafish embryogenesis. Development. 2015;142:2704-18 pubmed publisher
    ..Our results indicate novel roles for vertebrate Dchs in actin and microtubule cytoskeleton regulation in the unanticipated context of the single-celled embryo. ..
  7. Leichsenring M, Maes J, Mössner R, Driever W, Onichtchouk D. Pou5f1 transcription factor controls zygotic gene activation in vertebrates. Science. 2013;341:1005-9 pubmed publisher
    ..Our data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control. ..
  8. Shen Q, Little S, Xu M, Haupt J, Ast C, Katagiri T, et al. The fibrodysplasia ossificans progressiva R206H ACVR1 mutation activates BMP-independent chondrogenesis and zebrafish embryo ventralization. J Clin Invest. 2009;119:3462-72 pubmed publisher
  9. Pauli A, Montague T, Lennox K, Behlke M, Schier A. Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish. PLoS ONE. 2015;10:e0139504 pubmed publisher
    ..knockdown reproduced the published loss-of-function phenotypes for oep, chordin, dnd, ctnnb2, bmp7a, alk8, smad2 and smad5 in a dosage-sensitive manner...
  10. Tan Q, Balofsky A, Weisz K, Peng C. Role of activin, transforming growth factor-beta and bone morphogenetic protein 15 in regulating zebrafish oocyte maturation. Comp Biochem Physiol A Mol Integr Physiol. 2009;153:18-23 pubmed publisher
    ..Western blot analysis revealed that both activin-A and TGF-beta1 activate Smad2 in zebrafish follicles...
  11. Dick A, Mayr T, Bauer H, Meier A, Hammerschmidt M. Cloning and characterization of zebrafish smad2, smad3 and smad4. Gene. 2000;246:69-80 pubmed
    ..We report here the cloning, characterization and genomic mapping of smad2, smad3 and smad4 from the zebrafish, Danio rerio...
  12. Flanagan Steet H, Sias C, Steet R. Altered chondrocyte differentiation and extracellular matrix homeostasis in a zebrafish model for mucolipidosis II. Am J Pathol. 2009;175:2063-75 pubmed publisher
    ..These findings highlight the potential of the zebrafish system in studying lysosomal disease pathogenesis...
  13. Muller F, Albert S, Blader P, Fischer N, Hallonet M, Strahle U. Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. Development. 2000;127:3889-97 pubmed
    ..Modulation of the Cyc signalling pathway by constitutive activation or inhibition of Smad2 leads to altered shh expression in zebrafish embryos...
  14. Ma Y, Liu X, Liu Z, Wei S, Shang H, Xue Y, et al. The Chromatin Remodeling Protein Bptf Promotes Posterior Neuroectodermal Fate by Enhancing Smad2-Activated wnt8a Expression. J Neurosci. 2015;35:8493-506 pubmed publisher
    ..Bptf functionally and physically interacts with p-Smad2, which is activated by non-Nodal TGF-β signaling, to promote the expression of wnt8a, a critical gene for ..
  15. Sun H, Li D, Chen S, Liu Y, Liao X, Deng W, et al. Zili inhibits transforming growth factor-beta signaling by interacting with Smad4. J Biol Chem. 2010;285:4243-50 pubmed publisher
    ..interaction assays identify that Zili binds to Smad4 via its N-terminal domain and prevents the formation of Smad2/3/4 and Smad1/5/9/4 complexes to antagonize TGF-beta signaling...
  16. Pezeron G, Lambert G, Dickmeis T, Strahle U, Rosa F, Mourrain P. Rasl11b knock down in zebrafish suppresses one-eyed-pinhead mutant phenotype. PLoS ONE. 2008;3:e1434 pubmed publisher
    ..defects in other Nodal pathway mutants, nor does it influence the phosphorylation state of the downstream effector Smad2. Thus, Rasl11b modifies the effect of Oep on mesendoderm development independently of the main known Oep output: ..
  17. Kapsimali M, Caneparo L, Houart C, Wilson S. Inhibition of Wnt/Axin/beta-catenin pathway activity promotes ventral CNS midline tissue to adopt hypothalamic rather than floorplate identity. Development. 2004;131:5923-33 pubmed
    ..extracellular Wnt antagonists can promote hypothalamic gene expression when co-expressed with a modified form of Madh2 that activates Nodal signalling...
  18. Peng X, Li G, Wang Y, Zhuang J, Luo R, Chen J, et al. CXXC5 is required for cardiac looping relating to TGF? signaling pathway in zebrafish. Int J Cardiol. 2016;214:246-53 pubmed publisher
    ..This finding suggested that CXXC5 may serve as a possible marker that has potential diagnostic and prognostic value in fetus with congenital heart disease. ..
  19. Nelson A, Cutty S, Niini M, Stemple D, Flicek P, Houart C, et al. Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression. BMC Biol. 2014;12:81 pubmed publisher
    ..Using a combination of ChIP-seq and expression analysis we identify direct targets of Smad2, the effector of Nodal signalling in blastula stage zebrafish embryos, including many novel target genes...
  20. Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q. Fscn1 is required for the trafficking of TGF-? family type I receptors during endoderm formation. Nat Commun. 2016;7:12603 pubmed publisher
  21. Randall R, Germain S, Inman G, Bates P, Hill C. Different Smad2 partners bind a common hydrophobic pocket in Smad2 via a defined proline-rich motif. EMBO J. 2002;21:145-56 pubmed
    Transforming growth factor-beta (TGF-beta)/activin-induced Smad2/Smad4 complexes are recruited to different promoter elements by transcription factors, such as Fast-1 or the Mix family proteins Mixer and Milk, through a direct ..
  22. Van Boxtel A, Chesebro J, Heliot C, Ramel M, Stone R, Hill C. A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain. Dev Cell. 2015;35:175-85 pubmed publisher
    ..Here we use a specific zebrafish Nodal biosensor combined with immunofluorescence for phosphorylated Smad2 to demonstrate that endogenous Nodal is unlikely to diffuse over a long range...
  23. Batut J, Howell M, Hill C. Kinesin-mediated transport of Smad2 is required for signaling in response to TGF-beta ligands. Dev Cell. 2007;12:261-74 pubmed
    During vertebrate development, Activin/Nodal-related ligands signal through Smad2, leading to its activation and accumulation in the nucleus...
  24. Dubrulle J, Jordan B, Akhmetova L, Farrell J, Kim S, Solnica Krezel L, et al. Response to Nodal morphogen gradient is determined by the kinetics of target gene induction. elife. 2015;4: pubmed publisher
    ..expression patterns during zebrafish embryogenesis, we measured the activation dynamics of the signal transducer Smad2 and the expression kinetics of long- and short-range target genes...
  25. Bian S, Zheng X, Sun H, Chen J, Lu Y, Liu Y, et al. Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo. J Biol Chem. 2017;292:14165-14175 pubmed publisher
    Circadian clock and Smad2/3/4-mediated Nodal signaling regulate multiple physiological and pathological processes...
  26. Miyares R, Stein C, Renisch B, Anderson J, Hammerschmidt M, Farber S. Long-chain Acyl-CoA synthetase 4A regulates Smad activity and dorsoventral patterning in the zebrafish embryo. Dev Cell. 2013;27:635-47 pubmed publisher
    ..Our results reveal a critical role for Acsl4a in modulating Bmp-Smad activity and provide a potential avenue for LC-PUFAs to influence a variety of developmental processes. ..
  27. Maurus D, Harris W. Zic-associated holoprosencephaly: zebrafish Zic1 controls midline formation and forebrain patterning by regulating Nodal, Hedgehog, and retinoic acid signaling. Genes Dev. 2009;23:1461-73 pubmed publisher
    ..Repression of BMP signaling in dorsal forebrain has been implicated in causing MIH HPE. This work provides a mechanistical explanation at the molecular level of why Zic factors are associated with both major forms of HPE. ..
  28. Flanagan Steet H, Aarnio M, Kwan B, Guihard P, Petrey A, Haskins M, et al. Cathepsin-Mediated Alterations in TGFß-Related Signaling Underlie Disrupted Cartilage and Bone Maturation Associated With Impaired Lysosomal Targeting. J Bone Miner Res. 2016;31:535-48 pubmed publisher
    ..TGFß-like Smad2,3 signals are elevated and BMP-like Smad1,5,8 signals reduced in both feline and zebrafish MLII chondrocytes and ..
  29. Harvey S, Smith J. Visualisation and quantification of morphogen gradient formation in the zebrafish. PLoS Biol. 2009;7:e1000101 pubmed publisher
    ..In the first, we have used transgenic embryos to study the nuclear accumulation of a Smad2-Venus fusion protein, and in the second we have used bimolecular fluorescence complementation to visualise the ..