pcna

Summary

Gene Symbol: pcna
Description: proliferating cell nuclear antigen
Alias: cb16, fb36g03, wu:fa28e03, wu:fb36g03, etID36690.10, fa28e03
Species: zebrafish

Top Publications

  1. pmc The zebrafish mutants dre, uki, and lep encode negative regulators of the hedgehog signaling pathway
    Marco J Koudijs
    Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Utrecht, The Netherlands
    PLoS Genet 1:e19. 2005
  2. pmc Genetic dissection reveals two separate pathways for rod and cone regeneration in the teleost retina
    Ann C Morris
    Department of Biological Science, Florida State University, Tallahassee, Florida 32306, USA
    Dev Neurobiol 68:605-19. 2008
  3. pmc Distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development
    Sergey V Prykhozhij
    Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
    BMC Dev Biol 8:91. 2008
  4. ncbi Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish
    Shane M Fimbel
    Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Neurosci 27:1712-24. 2007
  5. pmc Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina
    Ryan Thummel
    Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Dev Neurobiol 68:392-408. 2008
  6. pmc Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation
    Anabela Bensimon-Brito
    PhD Program in Biomedical Sciences, University of Algarve, Faro, Portugal
    BMC Dev Biol 12:28. 2012
  7. pmc Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafish
    Kunal Rai
    Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84112, USA
    Genes Dev 21:261-6. 2007
  8. pmc Nestin is essential for zebrafish brain and eye development through control of progenitor cell apoptosis
    Hua Ling Chen
    Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan
    PLoS ONE 5:e9318. 2010
  9. pmc Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages
    Julia Mahler
    Developmental Biology Department, Institute of Biology I, University of Freiburg, Freiburg, Germany
    BMC Dev Biol 7:89. 2007
  10. ncbi Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells
    Rebecca L Bernardos
    Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Neurosci 27:7028-40. 2007

Research Grants

  1. NOVEL USE OF ZEBRAFISH FOR APOPTOSIS ASSAY
    Chuenlei Parng; Fiscal Year: 2004
  2. ASSAY FOR DETERMINING DRUG TOXICITY
    Chuenlei Parng; Fiscal Year: 2004
  3. Cardiotoxicity Drug Screening Using Zebrafish
    Chuenlei Parng; Fiscal Year: 2004
  4. Zebrafish Screen for Neuroprotective Anti-oxidants
    Chuenlei Parng; Fiscal Year: 2005
  5. Understanding fatty liver disease using the zebrafish mutant, foie gras
    KIRSTEN C SADLER EDEPLI; Fiscal Year: 2010

Scientific Experts

  • A Y H Leung
  • KIRSTEN C SADLER EDEPLI
  • Julia Mahler
  • Jenher Lu
  • Monica Sanden
  • Chuenlei Parng
  • Prisca Chapouton
  • Sean C Kassen
  • Sabine Seipold
  • Sergey V Prykhozhij
  • Ryan Thummel
  • Soojin Ryu
  • Øyvind Øverli
  • A C Morris
  • Susann Beetz
  • Kunal Rai
  • David R Hyde
  • Hyun Taek Kim
  • Jacob E Montgomery
  • David A Jones
  • Stephanie Chidester
  • Thomas Mueller
  • Katarzyna Koltowska
  • Hsin Yu Chung
  • Stefan H Oehlers
  • Anabela Bensimon-Brito
  • Dae gwon Ahn
  • Bastian Linder
  • Eriko Koshimizu
  • Koichi Fujisawa
  • Ling Zhang
  • Cheol Hee Kim
  • Claudia Seger
  • Christoph Winkler
  • Sonya E L Craig
  • Rosa A Uribe
  • Uta J Paulsen
  • Yoko Ito
  • Felicia S H Cheah
  • Takashi Ishida
  • Julia Ganz
  • Hua Ling Chen
  • Fumiyasu Imai
  • Lars Behrendt
  • Kristine von Krogh
  • Michael J Parsons
  • Peter F Hitchcock
  • Anda Alexandra Calinescu
  • Reid A Phelps
  • Natalia G Kan
  • Jennifer B Moss
  • Marcelo C Leal
  • Judith T M L Paridaen
  • Michael A Berberoglu
  • Suzanna L Gribble
  • Zhao Qin
  • Maren Mönnich
  • Adam R Karpf
  • Smitha R James
  • Bradley R Cairns
  • Danica Zivkovic
  • Michell M Reimer
  • Benson Wui Man Lau
  • Pamela A Raymond
  • Kanako Ito
  • Linda K Barthel
  • Thomas S Vihtelic
  • Vanesa Muncan
  • Chao Tsung Yang
  • Rebecca L Bernardos
  • Shane M Fimbel
  • Kirsten C Sadler
  • Hae Chul Park
  • Patrick Yurco
  • Elisabeth Pellegrini
  • N Plaster
  • T van der Meulen
  • Xiaohai Shi
  • Michael Schorpp
  • Helene Pendeville
  • Sara A Carney
  • John F Rawls
  • Erno Wienholds
  • Cecile Crosnier
  • Maren Ortiz-Zarragoitia
  • Marco J Koudijs
  • Mario F Wullimann
  • Elena Cid
  • Elke Rink
  • Huay Win Young

Detail Information

Publications77

  1. pmc The zebrafish mutants dre, uki, and lep encode negative regulators of the hedgehog signaling pathway
    Marco J Koudijs
    Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Utrecht, The Netherlands
    PLoS Genet 1:e19. 2005
    ..of proliferation, we performed a zebrafish genetic screen in which we used proliferating cell nuclear antigen (PCNA) expression as a readout. Two mutants, hu418B and hu540A, show increased PCNA expression...
  2. pmc Genetic dissection reveals two separate pathways for rod and cone regeneration in the teleost retina
    Ann C Morris
    Department of Biological Science, Florida State University, Tallahassee, Florida 32306, USA
    Dev Neurobiol 68:605-19. 2008
    ..These two genetic models therefore offer new opportunities for investigating the molecular mechanisms of selective degeneration and regeneration of rods and cones...
  3. pmc Distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development
    Sergey V Prykhozhij
    Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
    BMC Dev Biol 8:91. 2008
    ..Sonic hedgehog (Shh) directs pattern formation along the anterior/posterior axis of the limb, whereas several Fgfs in combination direct pattern formation along the proximal/distal axis of the limb...
  4. ncbi Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish
    Shane M Fimbel
    Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Neurosci 27:1712-24. 2007
    ..The regeneration response began within 1 dpi with increased proliferating cell nuclear antigen (PCNA) expression in both the INL and GCL. By 3 dpi, PCNA expression is primarily restricted to the Müller glia...
  5. pmc Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina
    Ryan Thummel
    Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Dev Neurobiol 68:392-408. 2008
    ..We determined that two separate morpholinos targeted against the proliferating cell nuclear antigen (PCNA) mRNA reduced PCNA protein levels...
  6. pmc Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation
    Anabela Bensimon-Brito
    PhD Program in Biomedical Sciences, University of Algarve, Faro, Portugal
    BMC Dev Biol 12:28. 2012
    ..This study compares initial patterns of mineralization in different regions of the vertebral column in zebrafish. We ask if the absence or presence of cartilaginous arches influences the pattern of notochord sheath mineralization...
  7. pmc Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafish
    Kunal Rai
    Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84112, USA
    Genes Dev 21:261-6. 2007
    ..Furthermore, zebrafish Dnmt2 methylates an RNA species of approximately 80 bases, consistent with tRNA methylation. Thus, Dnmt2 promotes zebrafish development, likely through cytoplasmic RNA methylation...
  8. pmc Nestin is essential for zebrafish brain and eye development through control of progenitor cell apoptosis
    Hua Ling Chen
    Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan
    PLoS ONE 5:e9318. 2010
    ..Nestin is expressed in neural progenitor cells (NPC) of developing brain. Despite its wide use as an NPC marker, the function of nestin in embryo development is unclear...
  9. pmc Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages
    Julia Mahler
    Developmental Biology Department, Institute of Biology I, University of Freiburg, Freiburg, Germany
    BMC Dev Biol 7:89. 2007
    ....
  10. ncbi Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells
    Rebecca L Bernardos
    Neuroscience Program, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Neurosci 27:7028-40. 2007
    ..We conclude that zebrafish Müller glia function as multipotent retinal stem cells that generate retinal neurons by homeostatic and regenerative developmental mechanisms...
  11. ncbi Zebrafish foxe3: roles in ocular lens morphogenesis through interaction with pitx3
    Xiaohai Shi
    Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA
    Mech Dev 123:761-82. 2006
    ..These data demonstrate that Foxe3 is necessary for lens development in zebrafish and that foxe3 lies genetically downstream of pitx3 in a zebrafish lens development pathway...
  12. ncbi Minichromosome maintenance proteins as markers for proliferation zones during embryogenesis
    Soojin Ryu
    Developmental Biology, Institute Biology 1, University of Freiburg, Freiburg, Germany
    Cell Cycle 5:1140-2. 2006
    ....
  13. ncbi p53 deficiency rescues apoptosis and differentiation of multiple cell types in zebrafish flathead mutants deficient for zygotic DNA polymerase delta1
    N Plaster
    Georges Köhler Laboratory, Max Planck Institute of Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany
    Cell Death Differ 13:223-35. 2006
    ..This indicates that under replication-compromised conditions, the p53 branch of the S-phase checkpoint is responsible for eliminating stalled cells that, given more time, would have otherwise finished their normal developmental program...
  14. pmc Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis
    Soojin Ryu
    Developmental Biology, Institute Biology 1, University of Freiburg, Hauptstrasse 1, D 79104 Freiburg, Germany
    Proc Natl Acad Sci U S A 102:18467-72. 2005
    ....
  15. doi Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish
    Takashi Ishida
    Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    Dev Biol 340:468-79. 2010
    ..Thus, our data suggest that the transcriptional and post-transcriptional controls of junbs and their protein products are the important regulative steps that enable tissue regeneration in zebrafish...
  16. doi Ethanol inhibits retinal and CNS differentiation due to failure of cell cycle exit via an apoptosis-independent pathway
    Hsin Yu Chung
    Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan
    Neurotoxicol Teratol 38:92-103. 2013
    ..Moreover, ethanol treatment enhanced BrdU incorporation, histone H3 phosphorylation, and pcna expression in neural progenitor cells, thereby maintaining a high rate of proliferation...
  17. pmc Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis
    Katarzyna Koltowska
    Developmental Biology, MRC National Institute for Medical Research, London, UK
    Development 140:1912-8. 2013
    ....
  18. pmc Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis
    Stefan H Oehlers
    Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland 1142, New Zealand
    Dis Model Mech 5:457-67. 2012
    ..This study illustrates a direct effect of retinoid administration on intestinal mucus physiology and, subsequently, on the progression of intestinal inflammation...
  19. pmc A novel model of retinal ablation demonstrates that the extent of rod cell death regulates the origin of the regenerated zebrafish rod photoreceptors
    Jacob E Montgomery
    Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Comp Neurol 518:800-14. 2010
    ..Second, the rod cell death must be acute, rather than chronic, to stimulate regeneration from the Müller glia. This suggests that the zebrafish retina possesses mechanisms to quantify the amount and timing of rod cell death...
  20. doi Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish
    Koichi Fujisawa
    Center for Reparative Medicine, Yamaguchi University School of Medicine, Minami Kogushi 1 1 1, Ube Yamaguchi 755 8505, Japan
    Biochem Biophys Res Commun 414:331-6. 2011
    ..Decreased expression was observed in foci, hepatocellular carcinomas, cholangiocellular carcinomas and mixed tumors as compared to the surrounding area. We thus showed the importance of SMP30/RGN in liver proliferation and tumorigenesis...
  21. pmc fezf2 expression delineates cells with proliferative potential and expressing markers of neural stem cells in the adult zebrafish brain
    Michael A Berberoglu
    Programs in Developmental Biology, Neuroscience and Human Genetics, Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158 2811, USA
    Gene Expr Patterns 9:411-22. 2009
    ....
  22. pmc Regeneration of the pancreas in adult zebrafish
    Jennifer B Moss
    Sarah W Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, North Carolina, USA
    Diabetes 58:1844-51. 2009
    ..We demonstrate the regeneration of adult zebrafish pancreatic beta-cells. This nonmammalian model can be used to define pathways for islet-cell regeneration in humans...
  23. pmc Compensatory growth mechanisms regulated by BMP and FGF signaling mediate liver regeneration in zebrafish after partial hepatectomy
    Natalia G Kan
    Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA
    FASEB J 23:3516-25. 2009
    ....
  24. pmc Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina
    Sabine Seipold
    Max Delbruck Center MDC for Molecular Medicine, Berlin, Germany
    BMC Dev Biol 9:40. 2009
    ..However, due to the lack of appropriate mutants, the function of the condensin I complex during vertebrate development has not been described...
  25. doi Dendritic differentiation in the periphery of the growing zebrafish retina
    Uta J Paulsen
    Anatomisches Institut, Universitat Tubingen, Osterbergstr 3, 72074 Tubingen, Germany
    Exp Eye Res 90:514-20. 2010
    ..Moreover, ganglion cells with specific branching patterns towards the retinal periphery undergo a restructuring of their dendritic trees...
  26. pmc The zebrafish galectin Drgal1-l2 is expressed by proliferating Müller glia and photoreceptor progenitors and regulates the regeneration of rod photoreceptors
    Sonya E L Craig
    Department of Ophthalmology and Visual Sciences, University of Michigan School of Medicine, Ann Arbor, Michigan 48105, USA
    Invest Ophthalmol Vis Sci 51:3244-52. 2010
    ....
  27. doi Expression of cohesin and condensin genes during zebrafish development supports a non-proliferative role for cohesin
    Maren Mönnich
    Department of Pathology, The University of Otago, Dunedin, New Zealand
    Gene Expr Patterns 9:586-94. 2009
    ..A comparison with pcna expression and BrdU incorporation revealed that the expression of cohesins and condensins closely overlap with ..
  28. pmc A two-step model for colon adenoma initiation and progression caused by APC loss
    Reid A Phelps
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Cell 137:623-34. 2009
    ..Consistent with this model, human FAP adenomas showed robust upregulation of CtBP1 in the absence of detectable nuclear beta-catenin, whereas nuclear beta-catenin was detected in carcinomas...
  29. pmc Genetic evidence for shared mechanisms of epimorphic regeneration in zebrafish
    Zhao Qin
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, 830 North University, Ann Arbor, MI 48109 1048, USA
    Proc Natl Acad Sci U S A 106:9310-5. 2009
    ..Comparison of gene expression profiles from regenerating zebrafish retina, caudal fin, and heart muscle revealed additional candidate genes potentially implicated in injury-induced epimorphic regeneration in diverse zebrafish tissues...
  30. pmc Dnmt3 and G9a cooperate for tissue-specific development in zebrafish
    Kunal Rai
    Department of Oncological Sciences, oward Hughes Medical Institute, and Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA
    J Biol Chem 285:4110-21. 2010
    ..We propose a model wherein specific DNMT-histone methyltransferase networks are utilized to silence critical regulators of cell fate in a tissue-specific manner...
  31. pmc Induction of cytochrome P450 1 genes and stress response genes in developing zebrafish exposed to ultraviolet radiation
    Lars Behrendt
    Biology Department, Redfield 352 MS 32, Woods Hole Oceanographic Institution, 45 Water Street, Woods Hole, MA 02543, USA
    Aquat Toxicol 98:74-82. 2010
    ..UVA alone had no effect on CYP1A expression. Proliferating cellular nuclear antigen (PCNA) and Cu-Zn superoxide dismutase (SOD1) transcript levels were induced (2.1+/-0.2 and 2.3+/-0...
  32. pmc Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos
    Jenher Lu
    Department of Pediatrics and Pathology, Taipei Veterans General Hospital, Taipei, Taiwan
    J Biomed Sci 18:73. 2011
    ..We report aberrant apoptosis and dormant cell growth over head, heart, trunk, fin, and tail of zebrafish embryos with tbx5 deficiency correspond to the dysmorphogenesis of tbx5 morphants...
  33. doi Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease
    Claudia Seger
    MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Firth Court, Western Bank, Sheffield, United Kingdom
    Dev Dyn 240:2440-51. 2011
    ..We also analyzed Pax7(+ve) cells in animals with dystrophic phenotypes and found an increased number compared with wild-type...
  34. pmc Cug2 is essential for normal mitotic control and CNS development in zebrafish
    Hyun Taek Kim
    Department of Biology, Chungnam National University, Daejeon, South Korea
    BMC Dev Biol 11:49. 2011
    ..However, the in vivo function of CUG2 has not been studied in animal models...
  35. pmc Embryonic senescence and laminopathies in a progeroid zebrafish model
    Eriko Koshimizu
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e17688. 2011
    ..Zebrafish show an array of senescence symptoms resembling those in humans, which can be targeted to specific aging pathways conserved in vertebrates. However, no zebrafish models bearing human premature senescence currently exist...
  36. doi Abnormal development of motor neurons in perfluorooctane sulphonate exposed zebrafish embryos
    Ling Zhang
    Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
    Ecotoxicology 20:643-52. 2011
    ..0 mg/L PFOS, then we examined the expression of alpha-tubulin, proliferating cell nuclear antigen (PCNA), cyclin-dependent kinase 5 (CDK5), and peroxiredoxin 2 (PRX2) after PFOS exposure until 120 hpf...
  37. doi Systemic splicing factor deficiency causes tissue-specific defects: a zebrafish model for retinitis pigmentosa
    Bastian Linder
    Department of Biochemistry, University of Wurzburg, Wurzburg, Germany
    Hum Mol Genet 20:368-77. 2011
    ..Hence, various routes affecting the tri-snRNP can elicit tissue-specific gene expression defects and lead to the RP phenotype...
  38. doi The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish
    Fumiyasu Imai
    Developmental Neurobiology Unit, Okinawa Institute of Science and Technology OIST, Onna, Okinawa, Japan
    Development 137:3257-68. 2010
    ..These data suggest that the ubiquitin proteasome system is required for cell proliferation of lens epithelium and for the differentiation of lens fiber cells in zebrafish...
  39. doi Heterogeneity and Fgf dependence of adult neural progenitors in the zebrafish telencephalon
    Julia Ganz
    Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 49, 01307 Dresden, Germany
    Glia 58:1345-63. 2010
    ....
  40. doi Notch activity levels control the balance between quiescence and recruitment of adult neural stem cells
    Prisca Chapouton
    Zebrafish Neurogenetics Department, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, D 85764 Neuherberg, Germany
    J Neurosci 30:7961-74. 2010
    ....
  41. pmc Id2a influences neuron and glia formation in the zebrafish retina by modulating retinoblast cell cycle kinetics
    Rosa A Uribe
    Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722, USA
    Development 137:3763-74. 2010
    ....
  42. doi Tgfbeta3 regulation of chondrogenesis and osteogenesis in zebrafish is mediated through formation and survival of a subpopulation of the cranial neural crest
    Felicia S H Cheah
    Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    Mech Dev 127:329-44. 2010
    ..Therefore, proper cranial neural crest formation and survival, and ultimately craniofacial chondrogenesis and osteogenesis, are dependent on tight regulation of Tgfbeta3 protein levels in zebrafish...
  43. doi Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum
    Yoko Ito
    Department of Life Science and Medical Bio Science, School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo, 162 8480, Japan
    Dev Biol 342:26-38. 2010
    ..These regions are distinguished by the differential expression of several marker genes: pcna, sox2, msi1, elavl3, gfap, fabp7a, and s100beta...
  44. doi Forebrain cell proliferation, behavior, and physiology of zebrafish, Danio rerio, kept in enriched or barren environments
    Kristine von Krogh
    Department of Production Animal Sciences, Norwegian School of Veterinary Science, P O Box 8146 Dep, N 0033 Oslo, Norway
    Physiol Behav 101:32-9. 2010
    ..barren) for one week. Telencephalic cell proliferation was investigated by proliferating cell nuclear antigen (PCNA) immunohistochemistry...
  45. doi CNTF induces photoreceptor neuroprotection and Müller glial cell proliferation through two different signaling pathways in the adult zebrafish retina
    Sean C Kassen
    Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA
    Exp Eye Res 88:1051-64. 2009
    ..Thus, CNTF utilizes a MAPK-dependant signaling pathway in neuroprotection of light-induced photoreceptor cell death and a Stat3-dependant signaling pathway to stimulate Müller glia proliferation...
  46. doi Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival
    Judith T M L Paridaen
    Hubrecht Institute, University Medical Center Utrecht, The Netherlands
    Dev Biol 331:101-12. 2009
    ..These data demonstrate that Apc1-mediated restriction of Wnt/beta-catenin signalling is required for maintenance of local organizers and tectal integrity...
  47. ncbi Both Hoxc13 orthologs are functionally important for zebrafish tail fin regeneration
    Ryan Thummel
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA
    Dev Genes Evol 217:413-20. 2007
    ..Hence, similar to the demonstration of unique functions of multiple Hox genes during limb formation, both Hoxc13 orthologs have distinct functions in regeneration...
  48. ncbi Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species
    Elena Cid
    Biologia Celular, INCYL, Facultad de Medicina, Campus Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain
    Cell Tissue Res 308:47-59. 2002
    ..Evaluation of cell proliferation was performed in proliferating cell nuclear antigen (PCNA)-labeled tissue sections as well as in single-cell suspensions analyzed by flow cytometry...
  49. ncbi Reciprocal gut microbiota transplants from zebrafish and mice to germ-free recipients reveal host habitat selection
    John F Rawls
    Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
    Cell 127:423-33. 2006
    ....
  50. ncbi her5 expression reveals a pool of neural stem cells in the adult zebrafish midbrain
    Prisca Chapouton
    Zebrafish Neurogenetics Junior Research Group, Institute of Virology, Technical University Munich, Trogerstrasse 4b, D 81675, Munich, Germany
    Development 133:4293-303. 2006
    ..In addition, the first transcription factor characteristic of this cell population, Her5, points to the E(Spl) as a promising family of candidate adult neural stem cell regulators...
  51. ncbi Endurance exercise differentially stimulates heart and axial muscle development in zebrafish (Danio rerio)
    T van der Meulen
    Experimental Zoology Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands
    Am J Physiol Regul Integr Comp Physiol 291:R1040-8. 2006
    ..In contrast, the heart muscle shifts to a faster phenotype but does not become more aerobic. This suggests that endurance training differentially affects heart and axial muscle...
  52. ncbi Conserved functions of Ikaros in vertebrate lymphocyte development: genetic evidence for distinct larval and adult phases of T cell development and two lineages of B cells in zebrafish
    Michael Schorpp
    Department of Developmental Immunology, Max Planck Institute of Immunobiology, D 79108 Freiburg, Germany
    J Immunol 177:2463-76. 2006
    ..By contrast, B cells expressing the igz isotype do not develop, providing genetic evidence for two separate B cell lineages in zebrafish. Thus, Ikaros appears to play similar roles in fish and mammalian lymphopoiesis...
  53. ncbi Aryl hydrocarbon receptor activation produces heart-specific transcriptional and toxic responses in developing zebrafish
    Sara A Carney
    Molecular and Environmental Toxicology Program, University of Wisconsin, Madison, Wisconsin 53705, USA
    Mol Pharmacol 70:549-61. 2006
    ..More than 70% of the transcripts in this heart-specific cluster promote cellular growth and proliferation. Thus, the developing heart stands out as being responsive to TCDD at both the level of toxicity and gene expression...
  54. doi The microcephaly gene aspm is involved in brain development in zebrafish
    Hyun Taek Kim
    Department of Biology and GRAST, Chungnam National University, Daejeon 305 764, Republic of Korea
    Biochem Biophys Res Commun 409:640-4. 2011
    ....
  55. ncbi A phylotypic stage in vertebrate brain development: GABA cell patterns in zebrafish compared with mouse
    Thomas Mueller
    Centre National de la Recherche Scientifique, Institute of Neurobiology A Fessard, Development, Evolution, and Plasticity of the Nervous System, Research Unit 2197, 91198 Gif sur Yvette, France
    J Comp Neurol 494:620-34. 2006
    ....
  56. ncbi Effects of selected xenoestrogens on liver peroxisomes, vitellogenin levels and spermatogenic cell proliferation in male zebrafish
    Maren Ortiz-Zarragoitia
    Biologia Zelularra eta Histologia Laborategia, Zoologia eta Biologia Zelularra Saila, Zientzia eta Teknologia Fakultatea, UPV EHU, 644 PK, E 48080 Bilbo, Basque Country, Spain
    Comp Biochem Physiol C Toxicol Pharmacol 141:133-44. 2005
    ..No such association occurred with typical peroxisome proliferators such as DBP...
  57. ncbi Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine
    Cecile Crosnier
    Vertebrate Development Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 132:1093-104. 2005
    ..These findings demonstrate the central role of Notch signalling in the gut stem-cell system and establish the zebrafish as a model for study of the mechanisms controlling renewal of gut epithelium...
  58. ncbi Development of the catecholaminergic system in the early zebrafish brain: an immunohistochemical study
    Elke Rink
    Brain Research Institute, University of Bremen, P O Box 33 04 40, D 28334 Bremen, Germany
    Brain Res Dev Brain Res 137:89-100. 2002
    ..preparations for visualizing TH cells and cells containing the proliferating cell nuclear antigen (PCNA cells) were carried out allowing for a prosomeric interpretation of early forebrain TH cell clusters...
  59. pmc Mutations in gfpt1 and skiv2l2 cause distinct stage-specific defects in larval melanocyte regeneration in zebrafish
    Chao Tsung Yang
    Department of Genetics, Washington University School of Medicine, Saint Louis, Missouri, United States
    PLoS Genet 3:e88. 2007
    ..We suggest that mechanisms of regeneration identified in this mutant screen may reveal fundamental differences between the mechanisms that establish differentiated cells during embryogenesis, and those involved in larval or adult growth...
  60. ncbi Characterization of terminal deoxynucleotidyl transferase and polymerase mu in zebrafish
    Susann Beetz
    Biology Department, Massachusetts Institute of Technology, Cambridge, MA, USA
    Immunogenetics 59:735-44. 2007
    ..The characterization of zebrafish TdT and polmu provide new insights in fish lymphopoiesis and addresses the importance and evolution of TdT and polmu themselves...
  61. pmc The Tg(ccnb1:EGFP) transgenic zebrafish line labels proliferating cells during retinal development and regeneration
    Sean C Kassen
    Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA
    Mol Vis 14:951-63. 2008
    ..To create the Tg(ccnb1:EGFP)(nt18) zebrafish line that spatially and temporally labels retinal progenitor cells with enhanced green fluorescent protein (EGFP) during zebrafish retinal development and regeneration...
  62. pmc Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression
    Suzanna L Gribble
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Development 136:781-9. 2009
    ..Both of these functions are mediated by Tcf3 independently of canonical Wnt signaling. Together, our data indicate a novel mechanism for the regulation of neurogenesis by Tcf3-mediated repression...
  63. pmc Cellular expression of midkine-a and midkine-b during retinal development and photoreceptor regeneration in zebrafish
    Anda Alexandra Calinescu
    Neuroscience Graduate Program, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Comp Neurol 514:1-10. 2009
    ....
  64. ncbi Molecular cloning and characterization of the zebrafish (Danio rerio) telomerase catalytic subunit (telomerase reverse transcriptase, TERT)
    Benson Wui Man Lau
    Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    J Mol Neurosci 34:63-75. 2008
    ..The expression of telomerase activity and TERT in retina implies that telomerase has functions other than the elongation of telomere. These findings could provide new insights on telomerase function in the nervous system...
  65. ncbi Macrophage migration inhibitory factor (MIF) is essential for development of zebrafish, Danio rerio
    Kanako Ito
    Laboratory of Fish Pathology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252 8510, Japan
    Dev Comp Immunol 32:664-72. 2008
    ..Moreover, proliferating cell nuclear antigen (PCNA)-positive cells were reduced in morphant eyes...
  66. ncbi An olig2 reporter gene marks oligodendrocyte precursors in the postembryonic spinal cord of zebrafish
    Hae Chul Park
    Graduate School of Medicine, Korea University, Ansan, Gyeonggido, Republic of Korea
    Dev Dyn 236:3402-7. 2007
    ..olig2(+) radial glia have hallmarks of stem cells and their divisions appear to be asymmetric, producing new oligodendrocytes but not neurons or astrocytes...
  67. doi Histological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generations
    Marcelo C Leal
    Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
    Biol Reprod 81:177-87. 2009
    ..Hence, the events taking place during puberty in the germinal epithelium of mammals seem to recapitulate the "life history" of each individual spermatogenic cyst in zebrafish...
  68. pmc T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine
    Vanesa Muncan
    Netherlands Institute for Developmental Biology, Center for Biomedical Research, Hubrecht Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    EMBO Rep 8:966-73. 2007
    ..This study underscores the involvement of Tcf4 in maintaining proliferative self-renewal in the intestine throughout life...
  69. ncbi Cellular correlates of proneural and Notch-delta gene expression in the regenerating zebrafish retina
    Patrick Yurco
    Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Vis Neurosci 24:437-43. 2007
    ..A model of vertebrate retinal repair is suggested in which damage-induced expression of proneural genes, plus canonical Notch-Delta signaling, could contribute to retinal stem cell promotion and subsequent regenerative neurogenesis...
  70. pmc Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration
    Ryan Thummel
    Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA
    Exp Eye Res 87:433-44. 2008
    ..We compared the temporal and spatial expression patterns of these markers relative to PCNA (proliferating cell nuclear antigen), an established marker for proliferating cells in the zebrafish retina, and ..
  71. ncbi Proliferating cell nuclear antigen (PCNA) as a proliferative marker during embryonic and adult zebrafish hematopoiesis
    Anskar Y H Leung
    Department of Medicine, Queen Mary Hospital, University of Hong Kong, Pok Fu Lam Road, Hong Kong, Hong Kong
    Histochem Cell Biol 124:105-11. 2005
    We investigated the expression of proliferative cell nuclear antigen (PCNA) in zebrafish to delineate the proliferative hematopoietic component during adult and embryonic hematopoiesis...
  72. doi Motor neuron regeneration in adult zebrafish
    Michell M Reimer
    Centre for Neuroscience Research, Royal Dick School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, United Kingdom
    J Neurosci 28:8510-6. 2008
    ....
  73. ncbi Cloning and embryonic expression of zebrafish PLAG genes
    Helene Pendeville
    Laboratoire de Biologie Moléculaire et Génie Génétique, Center for Biomedical Integrated Genoproteomics, University of Liege, B6 4000 Liège Sart Tilman, Belgium
    Gene Expr Patterns 6:267-76. 2006
    ..We show that this pattern resembles the one observed for the proliferative marker PCNA, suggesting that the PLAG genes are expressed more strongly in zones of active proliferation...
  74. ncbi Neurotoxicity assessment using zebrafish
    Chuenlei Parng
    Phylonix Pharmaceuticals, Inc, 100 Inman St, Cambridge, MA 02139, USA
    J Pharmacol Toxicol Methods 55:103-12. 2007
    ..Transparency is a unique attribute of zebrafish that permits direct assessment of drug effects on the nervous system using whole mount antibody immunostaining and histochemistry...
  75. ncbi Identification of aromatase-positive radial glial cells as progenitor cells in the ventricular layer of the forebrain in zebrafish
    Elisabeth Pellegrini
    Endocrinologie Moleculaire de la Reproduction, UMR CNRS 6026, Universite de Rennes 1, 35042 Rennes, France
    J Comp Neurol 501:150-67. 2007
    ....
  76. pmc Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1
    Kirsten C Sadler
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:1570-5. 2007
    ..This indicates that uhrf1 is required for physiologic liver growth in both embryos and adults and illustrates that zebrafish livers regenerate...

Research Grants5

  1. NOVEL USE OF ZEBRAFISH FOR APOPTOSIS ASSAY
    Chuenlei Parng; Fiscal Year: 2004
    ..Complementary visual and flow cytometry based assays will also be developed to provide comprehensive analysis of apoptosis in zebrafish. ..
  2. ASSAY FOR DETERMINING DRUG TOXICITY
    Chuenlei Parng; Fiscal Year: 2004
    ..abstract_text> ..
  3. Cardiotoxicity Drug Screening Using Zebrafish
    Chuenlei Parng; Fiscal Year: 2004
    ..Zebrafish drug screens can serve as an intermediate step between cell-based and mammalian testing, streamlining the drug development timeline. ..
  4. Zebrafish Screen for Neuroprotective Anti-oxidants
    Chuenlei Parng; Fiscal Year: 2005
    ..Assessment of drug effects in a convenient vertebrate model, prior to evaluation in a complex system, such as mouse, can potentially streamline the drug development process and dramatically reduce costs. ..
  5. Understanding fatty liver disease using the zebrafish mutant, foie gras
    KIRSTEN C SADLER EDEPLI; Fiscal Year: 2010
    ..This will conclusively demonstrate whether this pathway is protective or pathologic in the development of steatosis. ..