ndr2

Summary

Gene Symbol: ndr2
Description: nodal-related 2
Alias: Ndr-2, Znr-1, cyc, cyclops, ndr1, znr1, nodal-related 2, cyc-1, nodal-related 1
Species: zebrafish

Top Publications

  1. Bjornson C, Griffin K, Farr G, Terashima A, Himeda C, Kikuchi Y, et al. Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish. Dev Cell. 2005;9:523-33 pubmed
    ..Direct physical interactions between Eomes, Bon, and Gata5 suggest that Eomes promotes endoderm induction in marginal blastomeres by facilitating the assembly of a transcriptional activating complex on the cas promoter. ..
  2. Driever W, Solnica Krezel L, Schier A, Neuhauss S, Malicki J, Stemple D, et al. A genetic screen for mutations affecting embryogenesis in zebrafish. Development. 1996;123:37-46 pubmed
    ..This screen demonstrates that mutations affecting a variety of developmental processes can be efficiently recovered from zebrafish. ..
  3. Solnica Krezel L. Vertebrate development: taming the nodal waves. Curr Biol. 2003;13:R7-9 pubmed
  4. Gritsman K, Zhang J, Cheng S, Heckscher E, Talbot W, Schier A. The EGF-CFC protein one-eyed pinhead is essential for nodal signaling. Cell. 1999;97:121-32 pubmed
    ..An identical phenotype is displayed by double mutants for the nodal-related genes squint and cyclops. Mutations in oep eliminate the response to Squint and Cyclops overexpression but are suppressed by expression of ..
  5. Meno C, Gritsman K, Ohishi S, Ohfuji Y, Heckscher E, Mochida K, et al. Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. Mol Cell. 1999;4:287-98 pubmed
    ..Similarly, the effects of Antivin and Lefty2 can be suppressed by overexpression of the nodal-related genes cyclops and squint or the extracellular domain of ActRIIB...
  6. Bruce A, Howley C, Zhou Y, Vickers S, Silver L, King M, et al. The maternally expressed zebrafish T-box gene eomesodermin regulates organizer formation. Development. 2003;130:5503-17 pubmed
    ..In addition, a dominant-negative construct and antisense morpholino oligonucleotides led to a reduction in gsc and flh expression. Together these data indicate that eomes plays a role in specifying the organizer. ..
  7. Hatta K, Kimmel C, Ho R, Walker C. The cyclops mutation blocks specification of the floor plate of the zebrafish central nervous system. Nature. 1991;350:339-41 pubmed
    ..organized single cell row, but that its development fails in embryos bearing the newly discovered zygotic lethal 'cyclops' mutation, cyc-1(b16)...
  8. Bisgrove B, Essner J, Yost H. Multiple pathways in the midline regulate concordant brain, heart and gut left-right asymmetry. Development. 2000;127:3567-79 pubmed
    ..b>cyclops (nodal), lefty1 and pitx2 are expressed in the left diencephalon; cyclops, lefty2 and pitx2 are expressed in the ..
  9. Chen J, van Eeden F, Warren K, Chin A, Nusslein Volhard C, Haffter P, et al. Left-right pattern of cardiac BMP4 may drive asymmetry of the heart in zebrafish. Development. 1997;124:4373-82 pubmed
    ..Thus, the pattern of cardiac BMP4 appears to be in the pathway by which the heart interprets lateralizing signals from the midline. ..

More Information

Publications86

  1. Schötz E, Burdine R, Jülicher F, Steinberg M, Heisenberg C, Foty R. Quantitative differences in tissue surface tension influence zebrafish germ layer positioning. HFSP J. 2008;2:42-56 pubmed publisher
    ..We perform a mathematical analysis indicating that tissue interfacial tension between actively motile cells contributes to the spatial organization and dynamics of these zebrafish germ layers in vivo. ..
  2. Hong S, Jang M, Brown J, McBride A, Feldman B. Embryonic mesoderm and endoderm induction requires the actions of non-embryonic Nodal-related ligands and Mxtx2. Development. 2011;138:787-95 pubmed publisher
    ..an extraembryonic domain, are required for mesoderm and endoderm induction, and YSL expression of nodal-related 1 (ndr1) and ndr2 accounts for a portion of this activity...
  3. Jopling C, Hertog J. Essential role for Csk upstream of Fyn and Yes in zebrafish gastrulation. Mech Dev. 2007;124:129-36 pubmed
    ..The Csk knock down phenotype was rescued by simultaneous partial knock down of Fyn and Yes. We conclude that Csk acts upstream of Fyn and Yes to control vertebrate gastrulation cell movements. ..
  4. Brand M, Heisenberg C, Warga R, Pelegri F, Karlstrom R, Beuchle D, et al. Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. Development. 1996;123:129-42 pubmed
    ..gene is needed for early floor plate development, similar to one-eyed-pinhead (oep) and the previously described cyclops (cyc) gene...
  5. Hashimoto H, Rebagliati M, Ahmad N, Muraoka O, Kurokawa T, Hibi M, et al. The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish. Development. 2004;131:1741-53 pubmed publisher
    ..Charon also suppressed the dorsalizing activity of all three of the known zebrafish Nodal-related proteins (Cyclops, Squint and Southpaw), indicating that Charon can antagonize Nodal signaling...
  6. Maître J, Berthoumieux H, Krens S, Salbreux G, Jülicher F, Paluch E, et al. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 2012;338:253-6 pubmed publisher
    ..Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation. ..
  7. Tian J, Yam C, Balasundaram G, Wang H, Gore A, Sampath K. A temperature-sensitive mutation in the nodal-related gene cyclops reveals that the floor plate is induced during gastrulation in zebrafish. Development. 2003;130:3331-42 pubmed
    ..We describe a temperature-sensitive mutation that affects the zebrafish Nodal-related secreted signalling factor, Cyclops, and use it to address the issue of when the floor plate is induced in zebrafish...
  8. Thisse B, Wright C, Thisse C. Activin- and Nodal-related factors control antero-posterior patterning of the zebrafish embryo. Nature. 2000;403:425-8 pubmed
    ..We propose that modulation of intercellular signalling by Antivin/Activin and Nodal-related factors provides a mechanism for the graded establishment of cell fates along the antero-posterior axis of the zebrafish embryo. ..
  9. Lin F, Sepich D, Chen S, Topczewski J, Yin C, Solnica Krezel L, et al. Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements. J Cell Biol. 2005;169:777-87 pubmed
    ..These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation. ..
  10. Hagos E, Dougan S. Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish. BMC Dev Biol. 2007;7:22 pubmed
    ..In zebrafish, two nodal-related genes, called squint and cyclops, are required in a dosage-dependent manner for the formation of all derivatives of the mesoderm and endoderm...
  11. Solnica Krezel L, Stemple D, Mountcastle Shah E, Rangini Z, Neuhauss S, Malicki J, et al. Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish. Development. 1996;123:67-80 pubmed
    ..These mutations provide an approach to understanding the genetic control of gastrulation in vertebrates. ..
  12. Haffter P, Granato M, Brand M, Mullins M, Hammerschmidt M, Kane D, et al. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development. 1996;123:1-36 pubmed
    ..Here we give an overview of the spectrum of mutant phenotypes obtained, and discuss the limits and the potentials of a genetic saturation screen in the zebrafish. ..
  13. Fan X, Hagos E, Xu B, Sias C, Kawakami K, Burdine R, et al. Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish. Dev Biol. 2007;310:363-78 pubmed
    ..Targeted depletion experiments demonstrate that the zebrafish Nodal-related proteins Squint and Cyclops are required in the YSL for endoderm and head mesoderm formation...
  14. Dougan S, Warga R, Kane D, Schier A, Talbot W. The role of the zebrafish nodal-related genes squint and cyclops in patterning of mesendoderm. Development. 2003;130:1837-51 pubmed
    ..patterning roles for Nodal signals by using mutations in two zebrafish nodal-related genes, squint and cyclops, to manipulate genetically the levels and timing of Nodal activity...
  15. Gritsman K, Talbot W, Schier A. Nodal signaling patterns the organizer. Development. 2000;127:921-32 pubmed
    ..The nodal-related genes cyclops and squint are expressed at the blastoderm margin and are required for prechordal plate and notochord formation...
  16. Schier A, Neuhauss S, Harvey M, Malicki J, Solnica Krezel L, Stainier D, et al. Mutations affecting the development of the embryonic zebrafish brain. Development. 1996;123:165-78 pubmed
    ..Four loci were identified that affect dorsal-ventral patterning of the brain, including the previously described cyclops locus...
  17. Fauny J, Thisse B, Thisse C. The entire zebrafish blastula-gastrula margin acts as an organizer dependent on the ratio of Nodal to BMP activity. Development. 2009;136:3811-9 pubmed publisher
  18. Liao W, Ho C, Yan Y, Postlethwait J, Stainier D. Hhex and scl function in parallel to regulate early endothelial and blood differentiation in zebrafish. Development. 2000;127:4303-13 pubmed
    ..Together, these data provide the initial framework of a pathway that can be used to further integrate the molecular events regulating hemangioblast differentiation. ..
  19. Stemple D, Solnica Krezel L, Zwartkruis F, Neuhauss S, Schier A, Malicki J, et al. Mutations affecting development of the notochord in zebrafish. Development. 1996;123:117-28 pubmed
  20. Sidi S, Goutel C, Peyrieras N, Rosa F. Maternal induction of ventral fate by zebrafish radar. Proc Natl Acad Sci U S A. 2003;100:3315-20 pubmed
    ..They might further indicate that maternal TGF-betaRdr and WntCa(2+) pathways complementarily specify ventral cell fates, with the former triggering bmps expression and the latter indirectly repressing genes encoding BMP antagonists. ..
  21. Shimizu T, Bae Y, Muraoka O, Hibi M. Interaction of Wnt and caudal-related genes in zebrafish posterior body formation. Dev Biol. 2005;279:125-41 pubmed
    ..Of posterior body-formation genes, expression of the caudal-related cdx1a and cdx4/kugelig, but not bmps or cyclops, was strongly reduced in the wnt3a/wnt8 morphant embryos...
  22. Beis D, Stainier D. In vivo cell biology: following the zebrafish trend. Trends Cell Biol. 2006;16:105-12 pubmed
    ..Although this model system has been predominantly appreciated for its amenability to forward genetics, current advances in imaging technology and an increasing number of transgenic lines are bringing it closer to its full potential. ..
  23. Slagle C, Aoki T, Burdine R. Nodal-dependent mesendoderm specification requires the combinatorial activities of FoxH1 and Eomesodermin. PLoS Genet. 2011;7:e1002072 pubmed publisher
    ..Our findings also offer novel insights into the co-evolution of the Nodal signaling pathway, the notochord specification program, and the chordate branch of the deuterostome family of animals. ..
  24. Payumo A, Walker W, McQuade L, Yamazoe S, Chen J. Optochemical dissection of T-box gene-dependent medial floor plate development. ACS Chem Biol. 2015;10:1466-75 pubmed publisher
  25. Concha M, Burdine R, Russell C, Schier A, Wilson S. A nodal signaling pathway regulates the laterality of neuroanatomical asymmetries in the zebrafish forebrain. Neuron. 2000;28:399-409 pubmed
    ..This indicates that Nodal signaling is not required for asymmetric development per se but is essential to determine the laterality of the asymmetry. ..
  26. Sun H, Li D, Chen S, Liu Y, Liao X, Deng W, et al. Zili inhibits transforming growth factor-beta signaling by interacting with Smad4. J Biol Chem. 2010;285:4243-50 pubmed publisher
    ..This work shows that zili plays a role in early embryogenesis beyond germ line as a novel negative regulator of TGF-beta signaling, extending the function of Piwi proteins in vertebrates. ..
  27. Jing X, Zhou S, Wang W, Chen Y. Mechanisms underlying long- and short-range nodal signaling in Zebrafish. Mech Dev. 2006;123:388-94 pubmed
    ..In this study, we investigated the regulation of the signaling range of zebrafish Nodal proteins Cyclops and Squint, which are short- and long-range signals, respectively...
  28. Strahle U, Blader P, Ingham P. Expression of axial and sonic hedgehog in wildtype and midline defective zebrafish embryos. Int J Dev Biol. 1996;40:929-40 pubmed
    ..mesoderm; in contrast to flh and ntl mutations whose effects are restricted to the trunk and tail, mutation of cyclops (cyc) affects axl and shh expression along the entire midline of the neuroectoderm...
  29. Chocron S, Verhoeven M, Rentzsch F, Hammerschmidt M, Bakkers J. Zebrafish Bmp4 regulates left-right asymmetry at two distinct developmental time points. Dev Biol. 2007;305:577-88 pubmed
    ..The identification of these two distinct and opposing activities of BMP signaling provides new insight into how BMP signaling can regulate LR patterning. ..
  30. Maurus D, Harris W. Zic-associated holoprosencephaly: zebrafish Zic1 controls midline formation and forebrain patterning by regulating Nodal, Hedgehog, and retinoic acid signaling. Genes Dev. 2009;23:1461-73 pubmed publisher
    ..Repression of BMP signaling in dorsal forebrain has been implicated in causing MIH HPE. This work provides a mechanistical explanation at the molecular level of why Zic factors are associated with both major forms of HPE. ..
  31. Bian S, Zheng X, Sun H, Chen J, Lu Y, Liu Y, et al. Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo. J Biol Chem. 2017;292:14165-14175 pubmed publisher
  32. White R, Collins J, Sealy I, Wali N, Dooley C, Digby Z, et al. A high-resolution mRNA expression time course of embryonic development in zebrafish. elife. 2017;6: pubmed publisher
    ..To make this dataset a useful baseline reference, the data can be browsed and downloaded at Expression Atlas and Ensembl. ..
  33. Soroldoni D, Bajoghli B, Aghaallaei N, Czerny T. Dynamic expression pattern of Nodal-related genes during left-right development in medaka. Gene Expr Patterns. 2007;7:93-101 pubmed publisher
    ..Based on sequence analysis and in accordance to zebrafish orthologues we named the isolated genes Ndr1, Ndr2 and Spaw. Gene expression analysis throughout medaka development confirmed this assignment...
  34. Chen Y, Harris M, Levesque M, NUSSLEIN VOLHARD C, Sonawane M. Heterogeneity across the dorso-ventral axis in zebrafish EVL is regulated by a novel module consisting of sox, snail1a and max genes. Mech Dev. 2012;129:13-23 pubmed publisher
    ..For the first time, this transgenic line unravels the heterogeneity in the EVL and will serve as an important tool in understanding the molecular basis of the DV patterning of the EVL. ..
  35. Take uchi M, Clarke J, Wilson S. Hedgehog signalling maintains the optic stalk-retinal interface through the regulation of Vax gene activity. Development. 2003;130:955-68 pubmed
    ..Taking all these results together, we present a model of the partitioning of the optic vesicle along its proximo-distal axis. ..
  36. Pei W, Williams P, Clark M, Stemple D, Feldman B. Environmental and genetic modifiers of squint penetrance during zebrafish embryogenesis. Dev Biol. 2007;308:368-78 pubmed
    ..Two Nodal-related proteins - Squint (Sqt) and Cyclops (Cyc) - are expressed during germ-layer specification in zebrafish...
  37. Zhang C, Basta T, Hernandez Lagunas L, Simpson P, Stemple D, Artinger K, et al. Repression of nodal expression by maternal B1-type SOXs regulates germ layer formation in Xenopus and zebrafish. Dev Biol. 2004;273:23-37 pubmed
    ..Two nodal-related proteins are expressed in the early zebrafish embryo, squint and cyclops; antiSOX3c-injection leads to an increase in the level of cyclops expression...
  38. Varga Z, Wegner J, Westerfield M. Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops. Development. 1999;126:5533-46 pubmed
    ..In cyclops mutants, corresponding cells failed to move anteriorly, a ventral diencephalon never formed, and the eyes ..
  39. Du S, Draper B, Mione M, Moens C, Bruce A. Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A. Dev Biol. 2012;362:11-23 pubmed publisher
    ..We also show that Eomesa is required for normal expression of the endoderm markers sox32, bon and og9x; however it is not essential for endoderm formation. ..
  40. Cheng P, Lin C, Wu C, Lu Y, Lin C, Chung C, et al. Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation. Development. 2008;135:941-52 pubmed publisher
    ..This study underscores a novel role of zebrafish cdx1b in the development of different digestive organs compared with its mammalian homologs. ..
  41. Roussigné M, Bianco I, Wilson S, Blader P. Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development. 2009;136:1549-57 pubmed publisher
    ..Our results provide evidence of a direct requirement for unilateral Nodal activity in establishing an asymmetry per se, rather than solely in biasing its laterality. ..
  42. Chang L, Kessler D. Foxd3 is an essential Nodal-dependent regulator of zebrafish dorsal mesoderm development. Dev Biol. 2010;342:39-50 pubmed publisher
    ..Foxd3 gain-of-function results in expanded dorsal mesodermal gene expression, including the Nodal-related gene cyclops, and body axis dorsalization...
  43. Mangos S, Lam P, Zhao A, Liu Y, Mudumana S, Vasilyev A, et al. The ADPKD genes pkd1a/b and pkd2 regulate extracellular matrix formation. Dis Model Mech. 2010;3:354-65 pubmed publisher
    ..Our results suggest that pkd1a/b and pkd2 interact to regulate ECM secretion or assembly, and that altered matrix integrity may be a primary defect underlying ADPKD tissue pathologies. ..
  44. Chin A, Tsang M, Weinberg E. Heart and gut chiralities are controlled independently from initial heart position in the developing zebrafish. Dev Biol. 2000;227:403-21 pubmed
    ..Those with a relatively normal notochord (cyc(b16), din, and spt) displayed a predictive correlation between initial heart position and heart chirality, whereas ..
  45. Inbal A, Kim S, Shin J, Solnica Krezel L. Six3 represses nodal activity to establish early brain asymmetry in zebrafish. Neuron. 2007;55:407-15 pubmed
    ..Our data reveal a Six3-dependent mechanism for establishment of correct brain laterality and provide an entry point to understanding the genetic regulation of Nodal signaling in the brain. ..
  46. Van Boxtel A, Chesebro J, Heliot C, Ramel M, Stone R, Hill C. A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain. Dev Cell. 2015;35:175-85 pubmed publisher
    ..Thus, temporal information is transformed into spatial information to define the dimensions of the Nodal signaling domain and, consequently, to specify mesendoderm. ..
  47. Barreto Valer K, Lopez Bellido R, Rodriguez R. Cocaine modulates the expression of transcription factors related to the dopaminergic system in zebrafish. Neuroscience. 2013;231:258-71 pubmed publisher
    Nodal-related protein, Ndr2, and transcription factors such as Lmx1b, Otp, Nurr1 and Pitx3 are very important in the differentiation, function and maintenance of mesodiencephalic dopaminergic neurons, and are necessary for the activation ..
  48. Lin X, Rinaldo L, Fazly A, Xu X. Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis. Dev Biol. 2007;303:536-48 pubmed
    ..Thus, Med10 appears to be a unique MED subunit that differentially transduces information from distinct signaling pathways during zebrafish embryogenesis. ..
  49. Albert S, Muller F, Fischer N, Biellmann D, Neumann C, Blader P, et al. Cyclops-independent floor plate differentiation in zebrafish embryos. Dev Dyn. 2003;226:59-66 pubmed
    In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP)...
  50. Kenyon E, Campos I, Bull J, Williams P, Stemple D, Clark M. Zebrafish Rab5 proteins and a role for Rab5ab in nodal signalling. Dev Biol. 2015;397:212-24 pubmed publisher
    ..We conclude that rab5ab is essential for nodal signalling and organizer specification in the developing zebrafish embryo. ..
  51. Just S, Hirth S, Berger I, Fishman M, Rottbauer W. The mediator complex subunit Med10 regulates heart valve formation in zebrafish by controlling Tbx2b-mediated Has2 expression and cardiac jelly formation. Biochem Biophys Res Commun. 2016;477:581-588 pubmed publisher
    ..We propose here a rather unique role of Med10 in orchestrating cardiac valve formation by mediating Foxn4 dependent tbx2b transcription, expression of Has2 and subsequently proper development of the cardiac jelly. ..
  52. Khan A, Nakamoto A, Tai M, Saito S, Nakayama Y, Kawamura A, et al. Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis. Dev Growth Differ. 2012;54:686-701 pubmed publisher
    ..Our findings suggest that Pou2 functions in multiple aspects of vertebrate development, especially in the binary decision of the mesendoderm to mesoderm and endoderm in different ways depending on the developmental stage. ..
  53. Kang N, Won M, Rhee M, Ro H. Siah ubiquitin ligases modulate nodal signaling during zebrafish embryonic development. Mol Cells. 2014;37:389-98 pubmed publisher
    ..Additionally, since the embryos injected with Siah morpholinos mimicked the atv overexpression phenotype at least in part, our data support a model in which Siah is involved in mesendoderm patterning via modulating Nodal signaling. ..
  54. Essner J, Branford W, Zhang J, Yost H. Mesendoderm and left-right brain, heart and gut development are differentially regulated by pitx2 isoforms. Development. 2000;127:1081-93 pubmed
    ..Maintenance of pitx2c midline expression is dependent on cyclops (nodal) and schmalspur, but not no tail (brachyury)...
  55. Tessadori F, Noël E, Rens E, Magliozzi R, Evers van Gogh I, Guardavaccaro D, et al. Nodal signaling range is regulated by proprotein convertase-mediated maturation. Dev Cell. 2015;32:631-9 pubmed publisher
    ..Combined in silico and in vivo approaches support a model in which FurinA controls the signaling range of Spaw by cleaving its proprotein into a mature, extracellular form, consequently regulating left-right patterning. ..
  56. zhan G, Sezgin E, Wehner D, Pfister A, K hl S, Kagermeier Schenk B, et al. Lypd6 enhances Wnt/?-catenin signaling by promoting Lrp6 phosphorylation in raft plasma membrane domains. Dev Cell. 2013;26:331-45 pubmed publisher
    ..Thus, Lypd6 appears to control Lrp6 activation specifically in membrane rafts, which is essential for downstream signaling...
  57. Heisenberg C, Brand M, Jiang Y, Warga R, Beuchle D, van Eeden F, et al. Genes involved in forebrain development in the zebrafish, Danio rerio. Development. 1996;123:191-203 pubmed
    ..In an appendix to this study we list mutants showing alterations in the size of the eyes and abnormal differentiation of the lenses. ..
  58. Jopling C, van Geemen D, den Hertog J. Shp2 knockdown and Noonan/LEOPARD mutant Shp2-induced gastrulation defects. PLoS Genet. 2007;3:e225 pubmed
    ..The finding that defective Shp2 signaling induced cell movement defects as early as gastrulation may have implications for the monitoring and diagnosis of Noonan and LEOPARD syndrome...
  59. Malicki J, Neuhauss S, Schier A, Solnica Krezel L, Stemple D, Stainier D, et al. Mutations affecting development of the zebrafish retina. Development. 1996;123:263-73 pubmed
  60. Moreno Mateos M, Vejnar C, Beaudoin J, Fernandez J, Mis E, Khokha M, et al. CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Nat Methods. 2015;12:982-8 pubmed publisher
    ..These results identify determinants that influence Cas9 activity and provide a framework for the design of highly efficient sgRNAs for genome targeting in vivo. ..
  61. Li Villarreal N, Forbes M, Loza A, Chen J, Ma T, Helde K, et al. Dachsous1b cadherin regulates actin and microtubule cytoskeleton during early zebrafish embryogenesis. Development. 2015;142:2704-18 pubmed publisher
    ..Our results indicate novel roles for vertebrate Dchs in actin and microtubule cytoskeleton regulation in the unanticipated context of the single-celled embryo. ..
  62. Barth K, Miklosi A, Watkins J, Bianco I, Wilson S, Andrew R. fsi zebrafish show concordant reversal of laterality of viscera, neuroanatomy, and a subset of behavioral responses. Curr Biol. 2005;15:844-50 pubmed
    ..Revealing a link between visceral and brain asymmetry and lateralized behavior, our studies help to explain the complexity of the relationship between the lateralities of visceral and neural asymmetries. ..
  63. Zaucker A, Nagorska A, Kumari P, Hecker N, Wang Y, Huang S, et al. Translational co-regulation of a ligand and inhibitor by a conserved RNA element. Nucleic Acids Res. 2018;46:104-119 pubmed publisher
    ..3'UTR of zebrafish nodal-related1/squint (ndr1/sqt) ligand mRNA, is shared by the related ligand nodal-related2/cyclops (ndr2/cyc) and the nodal inhibitors, lefty1 (lft1) and lefty2 mRNAs...
  64. Machingo Q, Fritz A, Shur B. A beta1,4-galactosyltransferase is required for convergent extension movements in zebrafish. Dev Biol. 2006;297:471-82 pubmed
  65. Wang Y, Wang X, Wohland T, Sampath K. Extracellular interactions and ligand degradation shape the nodal morphogen gradient. elife. 2016;5: pubmed publisher
    ..By computational simulations of gradient formation, we demonstrate that diffusivity, extra-cellular interactions, and selective ligand destruction collectively shape the Nodal morphogen gradient. ..
  66. Talbot W, Egan E, Gates M, Walker C, Ullmann B, Neuhauss S, et al. Genetic analysis of chromosomal rearrangements in the cyclops region of the zebrafish genome. Genetics. 1998;148:373-80 pubmed
    ..in the analysis of these mutations, we genetically characterized three gamma-ray induced alleles of cyclops (cyc), a gene required for development of midline structures...
  67. Deshwar A, Chng S, Ho L, Reversade B, Scott I. The Apelin receptor enhances Nodal/TGF? signaling to ensure proper cardiac development. elife. 2016;5: pubmed publisher
    ..We demonstrate that loss of Aplnr attenuates the activity of a point source of Nodal ligands Squint and Cyclops in a non-cell autonomous manner...
  68. Fan X, Dougan S. The evolutionary origin of nodal-related genes in teleosts. Dev Genes Evol. 2007;217:807-13 pubmed
    ..Zebrafish have three nodal-related genes, called ndr1/squint, ndr2/cyclops, and ndr3/southpaw...
  69. Krens S, Veldhuis J, Barone V, Capek D, Maître J, Brodland G, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 2017;144:1798-1806 pubmed publisher
  70. Agathon A, Thisse C, Thisse B. The molecular nature of the zebrafish tail organizer. Nature. 2003;424:448-52 pubmed
    ..In contrast to induction of the vertebrate head, known to result from the triple inhibition of BMP, Nodal and Wnt, here we show that induction of the tail results from the triple stimulation of BMP, Nodal and Wnt8 signalling pathways. ..
  71. Tian J, Andrée B, Jones C, Sampath K. The pro-domain of the zebrafish Nodal-related protein Cyclops regulates its signaling activities. Development. 2008;135:2649-58 pubmed publisher
    ..Here, we characterize the zebrafish Nodal-related factor Cyclops (Cyc) and find unexpected functions for the pro-domain in regulating Cyc activity...
  72. Muto A, Calof A, Lander A, Schilling T. Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome. PLoS Biol. 2011;9:e1001181 pubmed publisher
  73. Warga R, Kane D. One-eyed pinhead regulates cell motility independent of Squint/Cyclops signaling. Dev Biol. 2003;261:391-411 pubmed
    ..However, in squint;cyclops double mutants, which lack Nodal function and possess a more severe phenotype than zygotic one-eyed pinhead ..
  74. Bisgrove B, Morelli S, Yost H. Genetics of human laterality disorders: insights from vertebrate model systems. Annu Rev Genomics Hum Genet. 2003;4:1-32 pubmed
    ..This approach helps suggest candidate genes and genetic pathways that might be perturbed in human laterality disorders and improves diagnostic criteria. ..
  75. Mizoguchi T, Izawa T, Kuroiwa A, Kikuchi Y. Fgf signaling negatively regulates Nodal-dependent endoderm induction in zebrafish. Dev Biol. 2006;300:612-22 pubmed
    ..Altogether, these results suggest that Fgf signaling negatively regulates endoderm induction, possibly through repression of cas expression and down-regulation of Cas function. ..
  76. Rohr K, Barth K, Varga Z, Wilson S. The nodal pathway acts upstream of hedgehog signaling to specify ventral telencephalic identity. Neuron. 2001;29:341-51 pubmed
    ..Thus, the Nodal pathway regulates ventral forebrain patterning through both Hedgehog signaling-dependent and -independent mechanisms. ..
  77. Shimizu T, Yamanaka Y, Ryu S, Hashimoto H, Yabe T, Hirata T, et al. Cooperative roles of Bozozok/Dharma and Nodal-related proteins in the formation of the dorsal organizer in zebrafish. Mech Dev. 2000;91:293-303 pubmed
    ..on both boz and sqt while the expression of din strongly depended on boz but only partially depended on sqt and cyclops (cyc, another nodal-related gene)...