Genomes and Genes
Gene Symbol: lhcgr
Description: luteinizing hormone/choriogonadotropin receptor
Alias: GTHR-II, lhr, zfGTHR-II, lutropin-choriogonadotropic hormone receptor, luteinizing hormone receptor
- Gioacchini G, Giorgini E, Merrifield D, Hardiman G, Borini A, Vaccari L, et al. Probiotics can induce follicle maturational competence: the Danio rerio case. Biol Reprod. 2012;86:65 pubmed publisher..Acquisition of competence by the stage IIIa follicles was further validated by changes of lhr, mprb, inhbaa (activin betaA1), tgfb1, and gdf9 gene expression, which have recently emerged as key regulators of ..
- Zhang Q, Li Y, Liu Z, Chen Q. Reproductive toxicity of inorganic mercury exposure in adult zebrafish: Histological damage, oxidative stress, and alterations of sex hormone and gene expression in the hypothalamic-pituitary-gonadal axis. Aquat Toxicol. 2016;177:417-24 pubmed publisher..ovarian 17?-estradiol (E2) content remained relatively stable, significant down-regulation of lh?, gnrh2, gnrh3, lhr and er? were observed...
- Xie Y, Chu L, Liu Y, Sham K, Li J, Cheng C. The highly overlapping actions of Lh signaling and Fsh signaling on zebrafish spermatogenesis. J Endocrinol. 2017;234:233-246 pubmed publisher..action of gonadotropin signaling on spermatogenesis by analyzing four single mutant lines (lhb, lhr, fshb and fshr) and three double mutant lines (lhb;fshb, lhr;fshr and fshb;lhrLiu K, Lau S, Ge W. Spatiotemporal expression analysis of nuclear estrogen receptors in the zebrafish ovary and their regulation in vitro by endocrine hormones and paracrine factors. Gen Comp Endocrinol. 2017;246:218-225 pubmed publisherEstradiol (E2) stimulates luteinizing hormone receptor (lhcgr) expression via nuclear estrogen receptors (nERs) in the zebrafish ovary...de Waal P, Leal M, García López A, Liarte S, De Jonge H, Hinfray N, et al. Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis. J Endocrinol. 2009;202:287-97 pubmed publisher..This experimental set-up helped to identify spermatogonial proliferation and their differentiation as androgen targets in adult zebrafish spermatogenesis. ..Maugars G, Dufour S. Demonstration of the Coexistence of Duplicated LH Receptors in Teleosts, and Their Origin in Ancestral Actinopterygians. PLoS ONE. 2015;10:e0135184 pubmed publisher..Until recently, it was accepted that gnathostomes possess a single FSHR and a single LHR, encoded by fshr and lhcgr genes. We reinvestigated this question, focusing on vertebrate species of key-phylogenetical positions...Liu W, Chen C, Chen L, Wang L, Li J, Chen Y, et al. Sex-dependent effects of microcystin-LR on hypothalamic-pituitary-gonad axis and gametogenesis of adult zebrafish. Sci Rep. 2016;6:22819 pubmed publisher..Major contributors to PC1 (gnrh2, gnrhr3, ar, lhr, hmgra, hmgrb and cyp19a) were positively correlated with the number of post-vitellogenic oocytes, while PC1 (gnrh2,..Zhang Z, Lau S, Zhang L, Ge W. Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males. Endocrinology. 2015;156:3747-62 pubmed publisher..Using the same approach, we successfully deleted FSH and LH receptor genes (fshr and lhcgr) in the present study...Chen W, Lau S, Fan Y, Wu R, Ge W. Juvenile exposure to bisphenol A promotes ovarian differentiation but suppresses its growth - Potential involvement of pituitary follicle-stimulating hormone. Aquat Toxicol. 2017;193:111-121 pubmed publisher..At the ovary level, BPA and E2 both decreased lhcgr expression. Interestingly, E2 and BPA displayed different effects in the liver...Kobayashi T, Andersen Ø. The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus), 1: isolation of multiple transcripts encoding full-length and truncated variants of FSH-R. Gen Comp Endocrinol. 2008;156:584-94 pubmed publisher..Based on the DNA sequence variation and chromosomal organization of the gonadotropin receptors in several teleosts, we propose that the encoding genes have been duplicated in the fish lineage. ..Li C, Ge W. Spatiotemporal expression of bone morphogenetic protein family ligands and receptors in the zebrafish ovary: a potential paracrine-signaling mechanism for oocyte-follicle cell communication. Biol Reprod. 2011;85:977-86 pubmed publisher..Further experiments using recombinant zebrafish Bmp4 showed that Bmp4 had an inhibitory effect on spontaneous oocyte maturation in vitro, but not 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP)-induced oocyte maturation in vitro. ..Han X, Lei E, Lam M, Wu R. A whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish. Mar Pollut Bull. 2011;63:160-5 pubmed publisher..The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. ..Cao F, Zhu L, Li H, Yu S, Wang C, Qiu L. Reproductive toxicity of azoxystrobin to adult zebrafish (Danio rerio). Environ Pollut. 2016;219:1109-1121 pubmed publisher..Meanwhile, expression alterations of genes encoding gonadotropins and gonadotropin receptors (fshb, lhb, fshr and lhr), steroid hormone receptors (era, er2b and ar), steroidogenic enzymes (cyp11a, cyp11b, cyp17, cyp19a, cyp19b, hsd3b ..Ji K, Hong S, Kho Y, Choi K. Effects of bisphenol s exposure on endocrine functions and reproduction of zebrafish. Environ Sci Technol. 2013;47:8793-800 pubmed publisher..Our observations showed that exposure to low level BPS could affect the feedback regulatory circuits of HPG axis and impair the development of offspring. ..García López A, De Jonge H, Nóbrega R, de Waal P, van Dijk W, Hemrika W, et al. Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins. Endocrinology. 2010;151:2349-60 pubmed publisher..that zebrafish Leydig cells as well as Sertoli cells express the mRNAs for both gonadotropin receptors (fshr and lhcgr)...Hou J, Li L, Wu N, Su Y, Lin W, Li G, et al. Reproduction impairment and endocrine disruption in female zebrafish after long-term exposure to MC-LR: A life cycle assessment. Environ Pollut. 2016;208:477-85 pubmed publisher..Significantly up-regulated transcription of brain FSHÎ² and LHÎ² along with ovarian ERÎ±, FSHR and LHR suggested positive feedback regulation in the HPGL-axis was induced as a compensatory mechanism for MC-LR damage...Shi J, Jiao Z, Zheng S, Li M, Zhang J, Feng Y, et al. Long-term effects of bisphenol AF (BPAF) on hormonal balance and genes of hypothalamus-pituitary-gonad axis and liver of zebrafish (Danio rerio), and the impact on offspring. Chemosphere. 2015;128:252-7 pubmed publisher..A potential consequence of adverse effects in the offspring by BPAF deserves further investigation. ..Dang Y, Wang J, Giesy J, Liu C. Responses of the zebrafish hypothalamic-pituitary-gonadal-liver axis PCR array to prochloraz are dependent on timing of sampling. Aquat Toxicol. 2016;175:154-9 pubmed publisher..Correlations among parameters in samples collected at the two times indicated the effects might be due to different concentrations of E2 in plasma due to exposure to PCZ. ..Liu K, Ge W. Evidence for gating roles of protein kinase A and protein kinase C in estradiol-induced luteinizing hormone receptor (lhcgr) expression in zebrafish ovarian follicle cells. PLoS ONE. 2013;8:e62524 pubmed publisherEstradiol (E2) stimulates luteinizing hormone receptor (lhcgr) expression in zebrafish follicle cells via nuclear estrogen receptors (nERs) that are likely expressed on the membrane, and lhcgr responds to E2 in a biphasic manner during 24-..Wong A, Van Eenennaam A. Gonadotropin hormone and receptor sequences from model teleost species. Zebrafish. 2004;1:203-21 pubmed publisher..approaches to identify the FSH and LH gonadotropin beta-subunits (fshb and lhb, respectively), and the LH receptor (lhr) from two model teleost species: zebrafish (Danio rerio) and Fugu (Takifugu rubripes)...Ji K, Liu X, Lee S, Kang S, Kho Y, Giesy J, et al. Effects of non-steroidal anti-inflammatory drugs on hormones and genes of the hypothalamic-pituitary-gonad axis, and reproduction of zebrafish. J Hazard Mater. 2013;254-255:242-51 pubmed publisher..Significant up-regulation of fsh?, lh?, fshr and lhr were observed in females, whereas down-regulation was observed in males exposed to each NSAID...Yumnamcha T, Khan Z, Rajiv C, Devi S, Mondal G, Sanjita Devi H, et al. Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis. Mol Reprod Dev. 2017;84:389-400 pubmed publisher..At the gonad level, expression of fshr, lhcgr, mtnr1aa, and mtnr1ab tracked with the expression of their respective ligand in DD and LL...Silva A, Almeida D, Nornberg B, Pereira J, Pires D, Corcini C, et al. Reproductive parameters of double transgenic zebrafish (Danio rerio) males overexpressing both the growth hormone (GH) and its receptor (GHR). Transgenic Res. 2017;26:123-134 pubmed publisher..Therefore, it is clear that GH-transgenesis technology should take into account the need to obtain adequate levels of circulating hormone in order to achieve maximum growth with minimal negative side effects. ..Lister A, Regan C, Van Zwol J, Van der Kraak G. Inhibition of egg production in zebrafish by fluoxetine and municipal effluents: a mechanistic evaluation. Aquat Toxicol. 2009;95:320-9 pubmed publisher..PCR analysis determined that ovarian aromatase, follicle stimulating hormone receptor (FSHr), and luteinizing hormone receptor (LHr) gene expression were significantly reduced by FLU suggesting that disruptions to the synthesis of ..Cosme M, Lister A, Van der Kraak G. Inhibition of spawning in zebrafish (Danio rerio): Adverse outcome pathways of quinacrine and ethinylestradiol. Gen Comp Endocrinol. 2015;219:89-101 pubmed publisher..and mature follicle stages), steroidogenesis (reduced expression of aromatase), maturation (reduced luteinizing hormone receptor expression) and ovulation (reduced expression of cytosolic phospholipase A2 and the nuclear ..Baudiffier D, Hinfray N, Ravaud C, Creusot N, Chadili E, Porcher J, et al. Effect of in vivo chronic exposure to clotrimazole on zebrafish testis function. Environ Sci Pollut Res Int. 2013;20:2747-60 pubmed publisher..Our study demonstrated that clotrimazole is able to affect testicular physiology and raised further concern about the impact of clotrimazole on reproduction. ..Carnevali O, Avella M, Gioacchini G. Effects of probiotic administration on zebrafish development and reproduction. Gen Comp Endocrinol. 2013;188:297-302 pubmed publisher..aromatase cytochrome p 19 (cyp19a), the vitellogenin (vtg) and the ? isoform of the E2 receptor (er?), luteinizing hormone receptor (lhr), 20-? hydroxysteroid dehydrogenase (20?-hsd), membrane progesterone receptors ? and ?, cyclin B, ..Santangeli S, Maradonna F, Zanardini M, Notarstefano V, Gioacchini G, Forner Piquer I, et al. Effects of diisononyl phthalate on Danio rerio reproduction. Environ Pollut. 2017;231:1051-1062 pubmed publisher..The findings provide evidence that exposure to DiNP adversely affect oocytes growth and maturation, leading to abnormal gonadal development and reproduction in zebrafish. ..Yang Q, Yang X, Liu J, Ren W, Chen Y, Shen S. Effects of BPF on steroid hormone homeostasis and gene expression in the hypothalamic-pituitary-gonadal axis of zebrafish. Environ Sci Pollut Res Int. 2017;24:21311-21322 pubmed publisher..These alterations suggest that BPF leads to adverse effects on the endocrine system of teleost fish, and that these effects were more prominent in males than in females. ..Liu K, Wu R, Ge W. Luteinizing hormone receptor (lhcgr) as a marker gene for characterizing estrogenic endocrine-disrupting chemicals in zebrafish ovarian follicle cells. Gen Comp Endocrinol. 2013;192:89-94 pubmed publisher..We have recently identified luteinizing hormone receptor (lhcgr) as a robust estradiol (E2)-responsive gene in cultured zebrafish ovarian follicle cells...Li X, Guo J, Li X, Zhou H, Zhang S, Liu X, et al. Behavioural effect of low-dose BPA on male zebrafish: Tuning of male mating competition and female mating preference during courtship process. Chemosphere. 2017;169:40-52 pubmed publisher..In sum, our results shed light on the potential behavioural and physiological effect of low-dose BPA exposure on courtship behaviours of zebrafish, which could exert profound consequences on natural zebrafish populations. ..Jo A, Ji K, Choi K. Endocrine disruption effects of long-term exposure to perfluorodecanoic acid (PFDA) and perfluorotridecanoic acid (PFTrDA) in zebrafish (Danio rerio) and related mechanisms. Chemosphere. 2014;108:360-6 pubmed publisher..Consequences of endocrine disruptions in reproduction performances of the fish warrant further investigation. ..Zhang W, Sheng N, Wang M, Zhang H, Dai J. Zebrafish reproductive toxicity induced by chronic perfluorononanoate exposure. Aquat Toxicol. 2016;175:269-76 pubmed publisher..The disrupted expression of genes, such as ER?, ER?, FSHR, LHR, StAR, and 17?HSD, indicated the possible interference of PFNA on the HPGL axis function and sex hormone synthesis...Qi X, Salem M, Zhou W, Sato Shimizu M, Ye G, Smitz J, et al. Neurokinin B Exerts Direct Effects on the Ovary to Stimulate Estradiol Production. Endocrinology. 2016;157:3355-65 pubmed publisher..Taken together, our findings establish a direct action of NKB to induce ovarian estrogen production and raise the possibility that defective signaling of this pathway may contribute to the development of PCOS. ..Chu L, Li J, Liu Y, Hu W, Cheng C. Targeted gene disruption in zebrafish reveals noncanonical functions of LH signaling in reproduction. Mol Endocrinol. 2014;28:1785-95 pubmed publisher..In this study, we have established both LH Î²-subunit (lhb) and LH receptor (lhr) knockout zebrafish lines by transcription activator-like effector nucleases...Poon S, So W, Yu X, Liu L, Ge W. Characterization of inhibin alpha subunit (inha) in the zebrafish: evidence for a potential feedback loop between the pituitary and ovary. Reproduction. 2009;138:709-19 pubmed publisher..The stimulation of inha expression by FSH and the potential inhibition of FSH by inhibin suggest a possible existence of a negative feedback loop between the pituitary and ovary in the zebrafish. ..Carnevali O, Gioacchini G, Maradonna F, Olivotto I, Migliarini B. Melatonin induces follicle maturation in Danio rerio. PLoS ONE. 2011;6:e19978 pubmed publisher..The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mpr? and mpr? gene transcription, whose proteins are involved in oocyte maturation...Tang H, Chen Y, Liu Y, Yin Y, Li G, Guo Y, et al. New Insights Into the Role of Estrogens in Male Fertility Based on Findings in Aromatase-Deficient Zebrafish. Endocrinology. 2017;158:3042-3054 pubmed publisher..Collectively, our findings indicate that estrogens are not needed to achieve and maintain normal fertility in male zebrafish. This finding challenges the traditional view that estrogens are indispensable for male fertility. ..Santangeli S, Maradonna F, Gioacchini G, Cobellis G, Piccinetti C, Dalla Valle L, et al. BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction. Sci Rep. 2016;6:21982 pubmed publisher..These data indicate that the negative effects of BPA on the female reproductive system may be due to its upstream ability to deregulate epigenetic mechanism. ..Hua J, Han J, Wang X, Guo Y, Zhou B. The binary mixtures of megestrol acetate and 17?-ethynylestradiol adversely affect zebrafish reproduction. Environ Pollut. 2016;213:776-784 pubmed publisher..This study has important implications for environmental risk assessment of synthetic hormones that are concurrently present in aquatic systems. ..Carnevali O, Tosti L, Speciale C, Peng C, Zhu Y, Maradonna F. DEHP impairs zebrafish reproduction by affecting critical factors in oogenesis. PLoS ONE. 2010;5:e10201 pubmed publisher..key regulators of oocyte maturation and ovulation including bone morphogenetic protein-15 (BMP15), luteinizing hormone receptor (LHR), membrane progesterone receptors (mPRs) and cyclooxygenase (COX)-2 (ptgs2) were determined by ..Gioacchini G, Maradonna F, Lombardo F, Bizzaro D, Olivotto I, Carnevali O. Increase of fecundity by probiotic administration in zebrafish (Danio rerio). Reproduction. 2010;140:953-9 pubmed publisher..was associated with increased transcription of genes coding for signals which induce the maturation phase, such as lhcgr, cbr1l, and paqr8 genes, concomitant with a decreased transcription of genes coding for local factors which ..Chu L, Li J, Liu Y, Cheng C. Gonadotropin Signaling in Zebrafish Ovary and Testis Development: Insights From Gene Knockout Study. Mol Endocrinol. 2015;29:1743-58 pubmed publisher..However, double knockout of fshb;lhb or fshr;lhr leads to all infertile male offspring...Nelson S, Van der Kraak G. The role of the insulin-like growth factor (IGF) system in zebrafish (Danio rerio) ovarian development. Gen Comp Endocrinol. 2010;168:103-10 pubmed publisher..These results provide evidence that the IGF system plays an important role in the promotion of oocyte maturation and ovarian development in the zebrafish. ..Heiden T, Struble C, Rise M, Hessner M, Hutz R, Carvan M. Molecular targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the zebrafish ovary: insights into TCDD-induced endocrine disruption and reproductive toxicity. Reprod Toxicol. 2008;25:47-57 pubmed..Data presented here provide further insight into the mechanisms by which TCDD disrupts follicular development and reproduction in fish, and can be used to formulate new hypotheses regarding previously documented ovarian toxicity. ..Li J, Liu Z, Wang D, Cheng C. Insulin-like growth factor 3 is involved in oocyte maturation in zebrafish. Biol Reprod. 2011;84:476-86 pubmed publisher..The actions of Igf3 could be blocked by cycloheximide, but not by actinomycin D. Taken together, these results support an important role of Igf3 in the ovarian functions of zebrafish, especially in oocyte maturation. ..Kohli G, Clelland E, Peng C. Potential targets of transforming growth factor-beta1 during inhibition of oocyte maturation in zebrafish. Reprod Biol Endocrinol. 2005;3:53 pubmed..20beta-HSD) which is involved in DHP production, follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), the two forms of membrane progestin receptor: mPR-alpha and mPR-beta, as well as GAPDH (control),..Tse A, Ge W. Spatial localization of EGF family ligands and receptors in the zebrafish ovarian follicle and their expression profiles during folliculogenesis. Gen Comp Endocrinol. 2010;167:397-407 pubmed publisher..The functional relationship between these two signaling systems in the follicle is suggested by the observation that all four EGFR ligands examined significantly stimulated activin subunit expression in cultured follicle cells. ..