kras

Summary

Gene Symbol: kras
Description: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Alias: K-ras, fa04e08, fc14b12, fc23g10, fj89d12, wu:fa04e08, wu:fc14b12, wu:fc23g10, wu:fj89d12, xRAS2, zgc:85725, GTPase KRas, ras-like protein
Species: zebrafish
Products:     kras

Top Publications

  1. Emelyanov A, Parinov S. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Dev Biol. 2008;320:113-21 pubmed publisher
    ..Combined with the Ac/Ds-mediated transgenesis, the LexPR expression system has many potential applications in the fields of genetics and biotechnology. ..
  2. Nguyen A, Emelyanov A, Koh C, Spitsbergen J, Parinov S, Gong Z. An inducible kras(V12) transgenic zebrafish model for liver tumorigenesis and chemical drug screening. Dis Model Mech. 2012;5:63-72 pubmed publisher
    ..by major hepatocellular carcinoma etiological factors, a transgenic zebrafish constitutively expressing the kras(V12) oncogene in the liver was previously generated by our laboratory...
  3. Liu L, Zhu S, Gong Z, Low B. K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis. PLoS ONE. 2008;3:e2850 pubmed publisher
  4. Seok S, Na Y, Han J, Kim T, Jung H, Lee B, et al. Cre/loxP-regulated transgenic zebrafish model for neural progenitor-specific oncogenic Kras expression. Cancer Sci. 2010;101:149-54 pubmed publisher
    ..In this study, we used the Cre/loxP system for maintaining transgenic zebrafish lines harboring oncogenic Kras(V12) under the nestin promoter, and investigated the developmental effects of Ras activation in neural progenitor ..
  5. Gill J, Lowe L, Nguyen J, Liu P, Blake T, Venkatesh B, et al. Enforced expression of Simian virus 40 large T-antigen leads to testicular germ cell tumors in zebrafish. Zebrafish. 2010;7:333-41 pubmed publisher
    ..In addition, enforced expression of the zebrafish stem cell leukemia (scl) gene in the zebrafish testes also generated TGCTs in transgenic fish. These results demonstrate the feasibility of studying TGCTs in a model organism. ..
  6. Razzaque M, Komoike Y, Nishizawa T, Inai K, Furutani M, Higashinakagawa T, et al. Characterization of a novel KRAS mutation identified in Noonan syndrome. Am J Med Genet A. 2012;158A:524-32 pubmed publisher
    ..Mutations in the Ras/mitogen-activated protein kinase (MAPK) signaling pathways cause about 70% of NS cases with a KRAS mutation present in about 2%...
  7. Yang Q, Yan C, Yin C, Gong Z. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol. 2017;3:484-499 pubmed publisher
    ..By using an inducible zebrafish HCC model through hepatocyte-specific transgenic krasV12 expression, differential rates of HCC in male and female fish were characterized by both ..
  8. Lockwood N, Parker J, Wilson C, Frankel P. Optimal Anesthetic Regime for Motionless Three-Dimensional Image Acquisition During Longitudinal Studies of Adult Nonpigmented Zebrafish. Zebrafish. 2017;14:133-139 pubmed publisher
    ..The anesthetic regime presented in this study should lead to significant improvements in accuracy and information achievable from imaging live adult zebrafish and in its application to longitudinal studies. ..
  9. Li Y, Li H, Spitsbergen J, Gong Z. Males develop faster and more severe hepatocellular carcinoma than females in krasV12 transgenic zebrafish. Sci Rep. 2017;7:41280 pubmed publisher
    ..and long-term tumor progression in our previously established inducible and reversible HCC model - the krasV12 transgenic zebrafish. We found that male fish developed HCC faster than females...

More Information

Publications26

  1. Semmelhack J, Donovan J, Thiele T, Kuehn E, Laurell E, Baier H. A dedicated visual pathway for prey detection in larval zebrafish. elife. 2014;3: pubmed publisher
    ..These findings indicate that computations in the retina give rise to a visual stream which transforms sensory information into a directed prey capture response. ..
  2. Kumar S, Lockwood N, Ramel M, Correia T, Ellis M, Alexandrov Y, et al. Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish. Oncotarget. 2016;7:43939-43948 pubmed publisher
    ..We believe that this instrument, together with its associated analysis and data management tools, constitute a new platform for in vivo cancer studies and drug discovery in zebrafish disease models. ..
  3. Barker A, Baier H. Sensorimotor decision making in the zebrafish tectum. Curr Biol. 2015;25:2804-2814 pubmed publisher
    ..We propose a model in which valence-based decisions arise, at a fundamental level, from competition between dedicated sensorimotor pathways in the tectum. ..
  4. Yao Y, Sun S, Fei F, Wang J, Wang Y, Zhang R, et al. Screening in larval zebrafish reveals tissue-specific distribution of fifteen fluorescent compounds. Dis Model Mech. 2017;10:1155-1164 pubmed publisher
    ..in the liver, and performed better in inhibiting hepatic hyperplasia caused by the over-expression of krasG12V In total, our study suggests that the transparent zebrafish larvae serve as valuable tools for ..
  5. Yan C, Yang Q, Huo X, Li H, Zhou L, Gong Z. Chemical inhibition reveals differential requirements of signaling pathways in krasV12- and Myc-induced liver tumors in transgenic zebrafish. Sci Rep. 2017;7:45796 pubmed publisher
    ..Thus, we evaluated the roles of several major signaling pathways in krasV12- or Myc-induced liver tumors by using several small molecule inhibitors: SU5402 and SU6668 for VEGF/..
  6. Yuan Y, Zhang J, Zhou Q. Overexpression of Jak1 Activating Mutants in Hepatocytes Is Insufficient to Generate Hepatocellular Carcinoma in Zebrafish. J Genet Genomics. 2016;43:99-102 pubmed publisher
  7. Nguyen A, Koh V, Spitsbergen J, Gong Z. Development of a conditional liver tumor model by mifepristone-inducible Cre recombination to control oncogenic kras V12 expression in transgenic zebrafish. Sci Rep. 2016;6:19559 pubmed publisher
    ..expression, mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic kras(V12)...
  8. Xiong C, Liu X, Meng A. The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo. J Biol Chem. 2015;290:25512-21 pubmed publisher
    ..Instead, Araf-tv2 interacts strongly with Kras and Nras, ultimately blocking MAPK activation by these Ras proteins...
  9. Yan C, Huo X, Wang S, Feng Y, Gong Z. Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. J Hepatol. 2015;63:420-8 pubmed publisher
    ..By inducible expression of oncogenic kras(V12) in hepatocytes in transgenic zebrafish combined with live imaging of neutrophils in transparent larvae, the ..
  10. Severi K, Portugues R, Marques J, O Malley D, Orger M, Engert F. Neural control and modulation of swimming speed in the larval zebrafish. Neuron. 2014;83:692-707 pubmed publisher
    ..By identifying the contribution of individual supraspinal circuit elements to locomotion kinematics, we build a better understanding of how the brain controls movement...
  11. Kubo F, Hablitzel B, Dal Maschio M, Driever W, Baier H, Arrenberg A. Functional architecture of an optic flow-responsive area that drives horizontal eye movements in zebrafish. Neuron. 2014;81:1344-1359 pubmed publisher
    ..Our data predict a wiring diagram of a neural circuit tailored to drive behavior that compensates for self-motion...
  12. Asaoka Y, Terai S, Sakaida I, Nishina H. The expanding role of fish models in understanding non-alcoholic fatty liver disease. Dis Model Mech. 2013;6:905-14 pubmed publisher
    ..The use of small fish in identifying novel potential therapeutic agents for the treatment of NAFLD and NASH is also addressed. ..
  13. Michailidou C, Jones M, Walker P, Kamarashev J, Kelly A, Hurlstone A. Dissecting the roles of Raf- and PI3K-signalling pathways in melanoma formation and progression in a zebrafish model. Dis Model Mech. 2009;2:399-411 pubmed publisher
  14. Temizer I, Donovan J, Baier H, Semmelhack J. A Visual Pathway for Looming-Evoked Escape in Larval Zebrafish. Curr Biol. 2015;25:1823-34 pubmed publisher
    ..Our data indicate that a subset of RGC axons within the tectum responds selectively to features of looming stimuli and that this input is necessary for visually evoked escape. ..
  15. Capon S, Baillie G, Bower N, da Silva J, Paterson S, Hogan B, et al. Utilising polymorphisms to achieve allele-specific genome editing in zebrafish. Biol Open. 2017;6:125-131 pubmed publisher
    ..These experiments demonstrate that single nucleotide polymorphisms (SNPs) present throughout the genome can be utilised to increase the efficiency of in cis genome editing using CRISPR/Cas9 in the zebrafish model. ..
  16. Chew T, Liu X, Liu L, Spitsbergen J, Gong Z, Low B. Crosstalk of Ras and Rho: activation of RhoA abates Kras-induced liver tumorigenesis in transgenic zebrafish models. Oncogene. 2014;33:2717-27 pubmed publisher
    ..an alternate cancer model, we have generated liver-specific, Tet-on-inducible transgenic lines expressing oncogenic Kras(G12V), RhoA, constitutively active RhoA(G14V) or dominant-negative RhoA(T19N)...
  17. Förster D, Arnold Ammer I, Laurell E, Barker A, Fernandes A, Finger Baier K, et al. Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools. Sci Rep. 2017;7:5230 pubmed publisher
    ..Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish. ..