itga2b

Summary

Gene Symbol: itga2b
Description: integrin, alpha 2b
Alias: si:dkey-27b3.6, integrin alpha-IIb, CD41, GPIIb, glycoprotein IIb, integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41B)
Species: zebrafish
Products:     itga2b

Top Publications

  1. Lin H, Traver D, Zhu H, Dooley K, Paw B, Zon L, et al. Analysis of thrombocyte development in CD41-GFP transgenic zebrafish. Blood. 2005;106:3803-10 pubmed
    ..We have cloned zebrafish CD41 cDNA (alpha(IIb), glycoprotein IIb [GPIIb]) and its promoter and have generated transgenic zebrafish lines with green fluorescent protein (GFP)-..
  2. Bertrand J, Kim A, Teng S, Traver D. CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis. Development. 2008;135:1853-62 pubmed publisher
    ..We have identified and prospectively isolated these cells by CD41 (itga2b) and cmyb expression...
  3. Keightley M, Carradice D, Layton J, Pase L, Bertrand J, Wittig J, et al. The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nat Commun. 2017;8:14911 pubmed publisher
    ..We demonstrate that the PU.1-ZBTB11-TP53 pathway is conserved from fish to mammals. Finally, Zbtb11 mutant rescue experiments point to a ZBTB11-regulated TP53 requirement in development of other organs. ..
  4. Lin Q, Zhou R, Zheng Y, Zhao L, Huang M, Zhang X, et al. Establishment of a congenital amegakaryocytic thrombocytopenia model and a thrombocyte-specific reporter line in zebrafish. Leukemia. 2017;31:1206-1216 pubmed publisher
    ..Together with Tg(mpl:eGFP)smu4, mpl mutant zebrafish provide a useful tool for drug screening and study of thrombocytopoiesis. ..
  5. Bertrand J, Chi N, Santoso B, Teng S, Stainier D, Traver D. Haematopoietic stem cells derive directly from aortic endothelium during development. Nature. 2010;464:108-11 pubmed publisher
    ..Finally, using a permanent lineage tracing strategy, we demonstrate that the HSCs generated from haemogenic endothelium are the lineal founders of the adult haematopoietic system. ..
  6. Sundaramoorthi H, Khandekar G, Kim S, Jagadeeswaran P. Knockdown of ?IIb by RNA degradation by delivering deoxyoligonucleotides piggybacked with control vivo-morpholinos into zebrafish thrombocytes. Blood Cells Mol Dis. 2015;54:78-83 pubmed publisher
    ..Thus, this novel piggyback technology can be utilized for cost-effective large-scale knockdowns of genes to study thrombocyte function in zebrafish. ..
  7. Pouget C, Peterkin T, Simões F, Lee Y, Traver D, Patient R. FGF signalling restricts haematopoietic stem cell specification via modulation of the BMP pathway. Nat Commun. 2014;5:5588 pubmed publisher
    ..These results should help inform strategies to recapitulate the development of HSCs in vitro from pluripotent precursors. ..
  8. Kim S, Radhakrishnan U, Rajpurohit S, Kulkarni V, Jagadeeswaran P. Vivo-Morpholino knockdown of alphaIIb: A novel approach to inhibit thrombocyte function in adult zebrafish. Blood Cells Mol Dis. 2010;44:169-74 pubmed publisher
    ..This study also provides the first antisense antithrombotic approach to inhibit thrombocyte function in adult zebrafish. ..
  9. Louwette S, Régal L, Wittevrongel C, Thys C, Vandeweeghde G, Decuyper E, et al. NPC1 defect results in abnormal platelet formation and function: studies in Niemann-Pick disease type C1 patients and zebrafish. Hum Mol Genet. 2013;22:61-73 pubmed publisher
    ..In conclusion, this is the first report, showing a role of NPC1 in platelet function and formation but further studies are needed to define how cholesterol storage interferes with these processes. ..

More Information

Publications29

  1. Soza Ried C, Hess I, Netuschil N, Schorpp M, Boehm T. Essential role of c-myb in definitive hematopoiesis is evolutionarily conserved. Proc Natl Acad Sci U S A. 2010;107:17304-8 pubmed publisher
    ..Our results, therefore, suggest that the key role of c-myb in definitive hematopoiesis is similar to that in mammals and must have become established early in vertebrate evolution. ..
  2. Bertrand J, Cisson J, Stachura D, Traver D. Notch signaling distinguishes 2 waves of definitive hematopoiesis in the zebrafish embryo. Blood. 2010;115:2777-83 pubmed publisher
    ..These studies establish that HSCs are the only hematopoietic precursor that requires Notch signaling and help to clarify the signaling events underlying the specification of the 2 distinct waves of definitive hematopoiesis. ..
  3. Zhao Y, Huang X, Ding T, Gong Z. Enhanced angiogenesis, hypoxia and neutrophil recruitment during Myc-induced liver tumorigenesis in zebrafish. Sci Rep. 2016;6:31952 pubmed publisher
    ..In sum, the current study demonstrated that the Myc-induced liver tumor model in zebrafish provides an excellent platform for study of tumor microenvironment. ..
  4. Woo A, Moran T, Schindler Y, Choe S, Langer N, Sullivan M, et al. Identification of ZBP-89 as a novel GATA-1-associated transcription factor involved in megakaryocytic and erythroid development. Mol Cell Biol. 2008;28:2675-89 pubmed publisher
  5. Ablooglu A, Kang J, Handin R, Traver D, Shattil S. The zebrafish vitronectin receptor: characterization of integrin alphaV and beta3 expression patterns in early vertebrate development. Dev Dyn. 2007;236:2268-76 pubmed
    ..Furthermore, when beta3.1 expression profiles are compared to those of other potential alphaV partners (beta1, beta5, and beta8), pharyngeal dentitions appear to represent a unique expression field for alphaV and beta3.1. ..
  6. Svoboda O, Stachura D, Machonova O, Zon L, Traver D, Bartunek P. Ex vivo tools for the clonal analysis of zebrafish hematopoiesis. Nat Protoc. 2016;11:1007-20 pubmed publisher
    ..The present protocol should facilitate such studies on cells derived from zebrafish. ..
  7. Svoboda O, Stachura D, Machonova O, Pajer P, Brynda J, Zon L, et al. Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Blood. 2014;124:220-8 pubmed publisher
    ..Tpo mRNA expanded itga2b:GFP(+) (cd41:GFP(+)) thrombocytes as well as hematopoietic stem and progenitor cells (HSPCs) in the zebrafish ..
  8. Page D, Wittamer V, Bertrand J, Lewis K, Pratt D, Delgado N, et al. An evolutionarily conserved program of B-cell development and activation in zebrafish. Blood. 2013;122:e1-11 pubmed publisher
    ..The IgM1:eGFP reporter fish are the first nonmammalian B-cell reporter animals to be described. They will be important for further investigation of immune cell evolution and development and host-pathogen interactions in zebrafish...
  9. Du L, Xu J, Li X, Ma N, Liu Y, Peng J, et al. Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development. 2011;138:619-29 pubmed publisher
    ..Our study identifies Rumba and Haus3 as two essential regulators of HSPC maintenance during zebrafish fetal hematopoiesis. ..
  10. Cooney J, Hildick Smith G, Shafizadeh E, McBride P, Carroll K, Anderson H, et al. Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Dev Biol. 2013;373:431-41 pubmed publisher
    ..Conversely, loss of gfi1b silences runx-1, c-myb, ikaros and cd41, indicating that gfi1b is required for definitive hematopoiesis...
  11. Louwette S, Labarque V, Wittevrongel C, Thys C, Metz J, Gijsbers R, et al. Regulator of G-protein signaling 18 controls megakaryopoiesis and the cilia-mediated vertebrate mechanosensory system. FASEB J. 2012;26:2125-36 pubmed publisher
    ..In summary, our study is the first to show that RGS18 regulates megakaryopoiesis but also reveals its unexpected role in ciliogenesis, at least in lower vertebrates, via interference with Wnt signaling. ..
  12. Kissa K, Herbomel P. Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature. 2010;464:112-5 pubmed publisher
  13. Amigo J, Ackermann G, Cope J, Yu M, Cooney J, Ma D, et al. The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish. Blood. 2009;114:4654-63 pubmed publisher
    ..One of these enhancers contains functional GATA-binding sites, indicating the potential for a regulatory loop in which GATA factors control the expression of their partner protein FOG-1. ..
  14. San Antonio J, Zoeller J, Habursky K, Turner K, Pimtong W, Burrows M, et al. A key role for the integrin alpha2beta1 in experimental and developmental angiogenesis. Am J Pathol. 2009;175:1338-47 pubmed publisher
    ..Collectively, our results provide strong biochemical and genetic evidence of a central role for alpha2beta1 integrin in experimental and developmental angiogenesis. ..
  15. Keightley M, Layton J, Hayman J, Heath J, Lieschke G. Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS ONE. 2011;6:e23845 pubmed publisher
    ..Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator. ..
  16. Zhou T, Wang L, Zhu K, Dong M, Xu P, Chen Y, et al. Dominant-negative C/ebp? and polycomb group protein Bmi1 extend short-lived hematopoietic stem/progenitor cell life span and induce lethal dyserythropoiesis. Blood. 2011;118:3842-52 pubmed publisher
    ..The results indicate that Bmi1 acts immediately downstream of C/ebp?DN to regulate the survival and self-renewal of HSPCs and contribute to the erythropoietic dysplasia. ..
  17. Tournoij E, Weber G, Akkerman J, de Groot P, Zon L, Moll F, et al. Mlck1a is expressed in zebrafish thrombocytes and is an essential component of thrombus formation. J Thromb Haemost. 2010;8:588-95 pubmed publisher
    ..5-fold enriched in platelets. Furthermore, mlck1a mRNA and mRNA for the platelet-specific cd41 co-localized in thrombi. Expression of other mlck subtypes was lower in GFP-tagged platelets (mlck1b; 0...
  18. Johnson H, Gandhi M, Shafizadeh E, Langer N, Pierce E, Paw B, et al. In vivo inactivation of MASTL kinase results in thrombocytopenia. Exp Hematol. 2009;37:901-8 pubmed publisher
    ..knockdown of MASTL in zebrafish correlates with a decrease in the expression of the thrombopoietin receptor, c-mpl, and the CD41 platelet adhesion protein, GpIIb, but has no effect on essential housekeeping zebrafish gene, EF1alpha.
  19. O Connor M, Salles I, Cvejic A, Watkins N, Walker A, Garner S, et al. Functional genomics in zebrafish permits rapid characterization of novel platelet membrane proteins. Blood. 2009;113:4754-62 pubmed publisher
    ..We conclude that phenotypic analysis of MO-injected zebrafish is a fast and powerful method for initial screening of novel platelet proteins for function in thrombosis. ..
  20. Zhu S, Zhang X, Weichert Leahey N, Dong Z, Zhang C, Lopez G, et al. LMO1 Synergizes with MYCN to Promote Neuroblastoma Initiation and Metastasis. Cancer Cell. 2017;32:310-323.e5 pubmed publisher
    ..and elevated expression levels of genes affecting tumor cell-extracellular matrix interaction, including loxl3, itga2b, itga3, and itga5...