Gene Symbol: irx3a
Description: iroquois homeobox 3a
Alias: hm:zehn0543, id:ibd3380, iro3, irx3, wu:fk88a03, zgc:109805, iroquois-class homeodomain protein IRX-3, iroquois homeobox protein 3a, zehn0543
Species: zebrafish

Top Publications

  1. Ragvin A, Moro E, Fredman D, Navratilova P, Drivenes Ø, Engström P, et al. Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3. Proc Natl Acad Sci U S A. 2010;107:775-80 pubmed publisher
    ..Knockdown of its orthologue in zebrafish, irx3a, increased the number of pancreatic ghrelin-producing epsilon cells and decreased the number of insulin-producing ..
  2. Tena J, Alonso M, de la Calle Mustienes E, Splinter E, de Laat W, Manzanares M, et al. An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation. Nat Commun. 2011;2:310 pubmed publisher
  3. Okigawa S, Mizoguchi T, Okano M, Tanaka H, Isoda M, Jiang Y, et al. Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination. Dev Biol. 2014;391:196-206 pubmed publisher
    ..In conclusion, V2-IN cell progenitor proliferation and V2a/V2b cell fate determination involve signaling through different sets of Notch ligand-receptor combinations that occur concurrently during development in zebrafish. ..
  4. Smemo S, Tena J, Kim K, Gamazon E, Sakabe N, G mez Mar n C, et al. Obesity-associated variants within FTO form long-range functional connections with IRX3. Nature. 2014;507:371-5 pubmed publisher
    ..noncoding sequences within FTO are functionally connected, at megabase distances, with the homeobox gene IRX3. The obesity-associated FTO region directly interacts with the promoters of IRX3 as well as FTO in the human, mouse ..
  5. Tanaka H, Morimura R, Ohshima T. Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish. Dev Biol. 2012;370:223-36 pubmed publisher
    ..These results suggest that the phosphorylation of Dpysl2 and Dpysl3 by Cdk5 and DYRK2 is required for the proper positioning of RB neurons and NCCs during neurulation in zebrafish embryos. ..
  6. Allende M, Manzanares M, Tena J, Feijóo C, Gomez Skarmeta J. Cracking the genome's second code: enhancer detection by combined phylogenetic footprinting and transgenic fish and frog embryos. Methods. 2006;39:212-9 pubmed
    ..Finally, for some of the most conserved elements, we have been able to identify a critical core sequence, essential for correct enhancer function. ..
  7. Guner B, Karlstrom R. Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube. Gene Expr Patterns. 2007;7:596-605 pubmed
  8. Bonner J, Gribble S, Veien E, Nikolaus O, Weidinger G, Dorsky R. Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling. Dev Biol. 2008;313:398-407 pubmed
    ..Together, our work demonstrates that proliferation and patterning in the developing spinal cord are separable events that are regulated independently by Wnt signaling. ..
  9. Batista M, Jacobstein J, Lewis K. Zebrafish V2 cells develop into excitatory CiD and Notch signalling dependent inhibitory VeLD interneurons. Dev Biol. 2008;322:263-75 pubmed publisher
    ..In addition, we demonstrate that Notch signalling is required for V2 cells to develop into V2b cells. In the absence of Notch signalling, all V2b cells develop as V2a cells. ..

More Information


  1. Gribble S, Kim H, Bonner J, Wang X, Dorsky R. Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Development. 2009;136:781-9 pubmed publisher
    ..Both of these functions are mediated by Tcf3 independently of canonical Wnt signaling. Together, our data indicate a novel mechanism for the regulation of neurogenesis by Tcf3-mediated repression. ..
  2. Rinkwitz S, Geng F, Manning E, Suster M, Kawakami K, Becker T. BAC transgenic zebrafish reveal hypothalamic enhancer activity around obesity associated SNP rs9939609 within the human FTO gene. Genesis. 2015;53:640-51 pubmed publisher
    ..tuberculum, the posterior hypothalamus and the anterior brainstem, which are also expression domains of zebrafish irx3a. In contrast, an in-frame insertion of a GFP cassette at the FTO start codon resulted in weak ubiquitous GFP ..
  3. Choy S, Cheng C, Lee S, Li V, Hui M, Hui C, et al. A cascade of irx1a and irx2a controls shh expression during retinogenesis. Dev Dyn. 2010;239:3204-14 pubmed publisher
    ..We proposed a novel transcriptional cascade of ath5-irx1a-irx2a in the regulation of hedgehog waves during vertebrate retinal development. ..
  4. Lewis K, Bates J, Eisen J. Regulation of iro3 expression in the zebrafish spinal cord. Dev Dyn. 2005;232:140-8 pubmed
    ..In this study, we analyze expression and regulation of the transcription factor iro3 in zebrafish spinal cord...
  5. McDonald L, Gerrelli D, Fok Y, Hurst L, Tickle C. Comparison of Iroquois gene expression in limbs/fins of vertebrate embryos. J Anat. 2010;216:683-91 pubmed publisher
    ..In zebrafish, Irx genes of all but six of the families are expressed in early stage pectoral fin buds but not at later stages, suggesting that these genes are not involved in patterning distal structures in zebrafish fins. ..
  6. Jeong J, Einhorn Z, Mathur P, Chen L, Lee S, Kawakami K, et al. Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl. Development. 2007;134:127-36 pubmed
    ..Our findings reveal that Fezl is crucial for establishing regional subdivisions within the diencephalon and may also play a role in the development of the telencephalon and hypothalamus. ..
  7. Lecaudey V, Anselme I, Dildrop R, Ruther U, Schneider Maunoury S. Expression of the zebrafish Iroquois genes during early nervous system formation and patterning. J Comp Neurol. 2005;492:289-302 pubmed
    ..Overall, these expression data suggest functions for the Iroquois family of transcription factors in neural and placodal patterning, neurogenesis, and neuronal specification. ..
  8. Bessa J, Luengo M, Rivero Gil S, Ariza Cosano A, Maia A, Ruiz Ruano F, et al. A mobile insulator system to detect and disrupt cis-regulatory landscapes in vertebrates. Genome Res. 2014;24:487-95 pubmed publisher
    ..We therefore provide a new easy-to-handle tool that will help to disrupt and chart the regulatory activity spread through the vast noncoding regions of the vertebrate genome. ..
  9. de la Calle Mustienes E, Feijóo C, Manzanares M, Tena J, Rodríguez Seguel E, Letizia A, et al. A functional survey of the enhancer activity of conserved non-coding sequences from vertebrate Iroquois cluster gene deserts. Genome Res. 2005;15:1061-72 pubmed
    ..However, inter-species conserved sequences surrounding the core, specific for each of these UCRs, are able to modulate their expression. ..
  10. Park H, Shin J, Appel B. Spatial and temporal regulation of ventral spinal cord precursor specification by Hedgehog signaling. Development. 2004;131:5959-69 pubmed
    ..Our studies show that spatial and temporal differences in Hh signaling within a common population of neural precursors can contribute to cell fate diversification. ..
  11. Bessa J, Tena J, de la Calle Mustienes E, Fernández Miñán A, Naranjo S, Fernandez A, et al. Zebrafish enhancer detection (ZED) vector: a new tool to facilitate transgenesis and the functional analysis of cis-regulatory regions in zebrafish. Dev Dyn. 2009;238:2409-17 pubmed publisher
    ..More strikingly, insulator sequences from mouse and chicken, but not conserved in zebrafish, maintain their insulator capacity when tested in this model. ..
  12. Feijóo C, Manzanares M, de la Calle Mustienes E, Gomez Skarmeta J, Allende M. The Irx gene family in zebrafish: genomic structure, evolution and initial characterization of irx5b. Dev Genes Evol. 2004;214:277-84 pubmed
    ..In total there are 11 existing Irx genes in zebrafish and 10 in pufferfish. We propose a new nomenclature for the zebrafish Irx genes based on the analysis of their sequences and their genomic relationships. ..
  13. Kim S, Kim S, Kim H, Chung A, Cha Y, Kim C, et al. Frizzled 8a function is required for oligodendrocyte development in the zebrafish spinal cord. Dev Dyn. 2008;237:3324-31 pubmed publisher
    ..In addition, we demonstrate that Wnt signaling activation after the specification of OPCs blocks the formation of mature oligodendrocytes and results in the elimination of OPCs. ..
  14. Zannino D, Downes G, SAGERSTROM C. prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Dev Biol. 2014;390:247-60 pubmed publisher
    ..We conclude that prdm12b is required for V1 interneuron specification and that these neurons control swimming movements in zebrafish. ..