Gene Symbol: gbx2
Description: gastrulation brain homeobox 2
Alias: fd07f12, gbx-2, wu:fd07f12, homeobox protein GBX-2, gastrulation brain homeo box 2
Species: zebrafish

Top Publications

  1. Rhinn M, Lun K, Ahrendt R, Geffarth M, Brand M. Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal. Neural Dev. 2009;4:12 pubmed publisher
    Studies in mouse, Xenopus and chicken have shown that Otx2 and Gbx2 expression domains are fundamental for positioning the midbrain-hindbrain boundary (MHB) organizer...
  2. Rhinn M, Lun K, Werner M, Simeone A, Brand M. Isolation and expression of the homeobox gene Gbx1 during mouse development. Dev Dyn. 2004;229:334-9 pubmed
    ..We suggest that an evolutionary switch occurred: in mouse Gbx2 is involved in the early specification of the MHB primordium, whereas in zebrafish, gbx1 is required instead of ..
  3. Rhinn M, Lun K, Amores A, Yan Y, Postlethwait J, Brand M. Cloning, expression and relationship of zebrafish gbx1 and gbx2 genes to Fgf signaling. Mech Dev. 2003;120:919-36 pubmed
    The organizer at the midbrain-hindbrain boundary (MHB) forms at the interface between Otx2 and Gbx2 expressing cell populations, but how these gene expression domains are set up and integrated with the remaining machinery controlling MHB ..
  4. Kikuta H, Kanai M, Ito Y, Yamasu K. gbx2 Homeobox gene is required for the maintenance of the isthmic region in the zebrafish embryonic brain. Dev Dyn. 2003;228:433-50 pubmed
    We isolated cDNA clones for the zebrafish gbx2 gene, which is implicated in the establishment of the midbrain-hindbrain boundary (MHB) in other vertebrates...
  5. Islam M, Kikuta H, Inoue F, Kanai M, Kawakami A, Parvin M, et al. Three enhancer regions regulate gbx2 gene expression in the isthmic region during zebrafish development. Mech Dev. 2006;123:907-24 pubmed
    ..In zebrafish, sequential expression of gbx1 and gbx2 in the anterior hindbrain contributes to this process, whereas in mouse embryos, a single Gbx gene (Gbx2) is ..
  6. Foucher I, Mione M, Simeone A, Acampora D, Bally Cuif L, Houart C. Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants. Development. 2006;133:1891-900 pubmed
    ..This maintenance is enough to allow cerebellar differentiation. ..
  7. Klangnurak W, Tokumoto T. Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish. Zoological Lett. 2017;3:2 pubmed publisher
    ..The ovulation-specific up-regulation of three candidates, slc37a4a, zgc:65811 and zgc:92184 was confirmed by qPCR. Our in vivo assay provides a new approach to precisely select genes associated with ovulation. ..
  8. Emoto Y, Wada H, Okamoto H, Kudo A, Imai Y. Retinoic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish. Dev Biol. 2005;278:415-27 pubmed
    ..We propose a model in which Cyp26a1 attenuates RA signaling in the prospective rostral spinal cord to limit the expression of hox genes and to determine the hindbrain-spinal cord boundary. ..
  9. Langenberg T, Brand M. Lineage restriction maintains a stable organizer cell population at the zebrafish midbrain-hindbrain boundary. Development. 2005;132:3209-16 pubmed
    ..Our findings suggest that segmentation as an organizing principle in early brain development can be extended to the mhb region. We argue that lineage restriction serves to constrain the position of the mhb organizer cell population. ..

More Information


  1. Abbas L, Whitfield T. Nkcc1 (Slc12a2) is required for the regulation of endolymph volume in the otic vesicle and swim bladder volume in the zebrafish larva. Development. 2009;136:2837-48 pubmed publisher
    ..This exemplifies the use of morpholinos as potential therapeutic agents for genetic disease...
  2. Burroughs Garcia J, Sittaramane V, Chandrasekhar A, Waters S. Evolutionarily conserved function of Gbx2 in anterior hindbrain development. Dev Dyn. 2011;240:828-38 pubmed publisher
    The amino acid sequence across the DNA-binding homeodomain of Gbx2 is highly conserved across multiple species...
  3. Su C, Kemp H, Moens C. Cerebellar development in the absence of Gbx function in zebrafish. Dev Biol. 2014;386:181-90 pubmed publisher
    ..The MHB forms at the expression boundary of Otx2 and Gbx2, mutually repressive homeodomain transcription factors expressed in the midbrain/forebrain and anterior hindbrain, ..
  4. Erickson T, Scholpp S, Brand M, Moens C, Waskiewicz A. Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Dev Biol. 2007;301:504-17 pubmed
    ..Embryos lacking Pbx function correctly initiate midbrain patterning, but fail to maintain eng2a, pax2a, fgf8, gbx2, and wnt1 expression at the MHB...
  5. Peukert D, Weber S, Lumsden A, Scholpp S. Lhx2 and Lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating Wnt signaling. PLoS Biol. 2011;9:e1001218 pubmed publisher
    ..We therefore suggest that after initial neural tube patterning, neurogenesis within a brain compartment influences the integrity of the neuronal progenitor pool and border formation of a neuromeric compartment. ..
  6. Tuttle A, Hoffman T, Schilling T. Rabconnectin-3a regulates vesicle endocytosis and canonical Wnt signaling in zebrafish neural crest migration. PLoS Biol. 2014;12:e1001852 pubmed publisher
    ..Our results suggest that different v-ATPases and associated proteins may play cell-type-specific functions in intracellular trafficking in many contexts...
  7. Albuixech Crespo B, López Blanch L, Burguera D, Maeso I, Sánchez Arrones L, Moreno Bravo J, et al. Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain. PLoS Biol. 2017;15:e2001573 pubmed publisher
    ..This suggests that these domains have a common developmental and evolutionary origin, as supported by functional experiments manipulating secondary organizers in zebrafish and mice. ..
  8. Nakayama Y, Kikuta H, Kanai M, Yoshikawa K, Kawamura A, Kobayashi K, et al. Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner. Mech Dev. 2013;130:532-52 pubmed publisher
    ..we first examined the dose dependency of its effects on brain formation in zebrafish and found that high-dose gbx2 mRNA injection affected the entire forebrain and midbrain, whereas low-dose mRNA specifically disrupted the isthmic ..
  9. Sun Z, Zhao J, Zhang Y, Meng A. Sp5l is a mediator of Fgf signals in anteroposterior patterning of the neuroectoderm in zebrafish embryo. Dev Dyn. 2006;235:2999-3006 pubmed inhibit inductive activity of ectopic Fgf signals on the expression of the posterior neuroectodermal markers gbx2, hoxb1b, and krox20...