efnb2a

Summary

Gene Symbol: efnb2a
Description: ephrin B2a
Alias: efnb2, ephrin-B2a, id:ibd5072, ephrin-B2a
Species: zebrafish

Top Publications

  1. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
  2. ncbi Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 445:781-4. 2007
  3. pmc Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
    Shane P Herbert
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA
    Science 326:294-8. 2009
  4. ncbi Sox18 and Sox7 play redundant roles in vascular development
    Solei Cermenati
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, Milan, Italy
    Blood 111:2657-66. 2008
  5. ncbi sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
  6. pmc Semaphorin-PlexinD1 signaling limits angiogenic potential via the VEGF decoy receptor sFlt1
    Tomasz Zygmunt
    Department of Cell Biology, Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Dev Cell 21:301-14. 2011
  7. ncbi fused-somites-like mutants exhibit defects in trunk vessel patterning
    Kenna M Shaw
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Dyn 235:1753-60. 2006
  8. pmc Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates
    Chang Z Chun
    Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Blood 113:1192-9. 2009
  9. ncbi EphA4 is required for cell adhesion and rhombomere-boundary formation in the zebrafish
    Julie E Cooke
    Howard Hughes Medical Institute and Division of Basic Science, Fred Hutchinson Cancer Research Center B2 152, 1100 Fairview Avenue N, P O Box 19024, Seattle, Washington 98109, USA
    Curr Biol 15:536-42. 2005
  10. ncbi Eph/Ephrin signaling regulates the mesenchymal-to-epithelial transition of the paraxial mesoderm during somite morphogenesis
    Arantza Barrios
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    Curr Biol 13:1571-82. 2003

Scientific Experts

  • Chun Guang Ren
  • Shane P Herbert
  • Xabier L Aranguren
  • Suk Won Jin
  • Lisa Maves
  • L Durbin
  • J Cooke
  • Nathan D Lawson
  • Arantza Barrios
  • Joanne Chan
  • Jeffrey M Gross
  • Masanori Hirashima
  • Andrew C Oates
  • Scot A Wolfe
  • Brant M Weinstein
  • Jesus Torres-Vazquez
  • Leonard I Zon
  • Fatma O Kok
  • L D Covassin
  • Van N Pham
  • Dörthe Jülich
  • Timothy Erickson
  • Curtis R French
  • Chi Bin Chien
  • Stefan Schulte-Merker
  • Jonathan A Epstein
  • Arndt F Siekmann
  • Qin Li
  • Martin Gering
  • Hitoshi Okamoto
  • Heng Huang
  • Se Hee Kim
  • Yong Zhang
  • Di Chen
  • Simone Lackner
  • Makoto Kamei
  • Renee Kruse-Bend
  • Shannon McKinney-Freeman
  • Shunmoogum A Patten
  • Randall T Peterson
  • Kevin Mouillesseaux
  • Tomasz Zygmunt
  • Lu Wang
  • Cong Zhu
  • Xiaoyang Wan
  • Scott A Holley
  • Karlien Hermans
  • Jie Mei
  • Jonathan M Rowlinson
  • Yingdi Wang
  • Charles Williams
  • Peng chun Yu
  • Andrew J Pittman
  • Ryan E Lamont
  • Christina Therapontos
  • Andrew J Waskiewicz
  • Sarah Baxendale
  • Nathan J Gosse
  • Elisabetta Dejana
  • Chang Z Chun
  • CAROLINE E BURNS
  • Trista E North
  • Hilary A Kemp
  • Aline Stedman
  • Howard I Sirotkin
  • Solei Cermenati
  • Filip Claes
  • Monica Autiero
  • Benjamin M Hogan
  • Peter Carmeliet
  • Michael Simons
  • Robert Herpers
  • Fiona Rodriguez
  • Helene Pendeville
  • Roger Patient
  • Annette Schenck
  • Ken ichi Minehata
  • Stefania Nicoli
  • J A Villefranc
  • B M Weinstein
  • M C Kacergis
  • Seok Hyung Kim
  • Calum A Macrae
  • Tao P Zhong
  • Tomomi Sato
  • Jonathan D Leslie
  • Cecilia B Moens
  • Thomas W Chittenden
  • Anandita Seth
  • Kenna M Shaw

Detail Information

Publications86

  1. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
    ..These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish...
  2. ncbi Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 445:781-4. 2007
    ..Together, these studies indicate that proper specification of cell identity, position and behaviour in a developing blood-vessel sprout is required for normal angiogenesis, and implicate the Notch signalling pathway in this process...
  3. pmc Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
    Shane P Herbert
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA
    Science 326:294-8. 2009
    ..Thus, directional control of progenitor migration drives arterial-venous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development...
  4. ncbi Sox18 and Sox7 play redundant roles in vascular development
    Solei Cermenati
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, Milan, Italy
    Blood 111:2657-66. 2008
    ..Our data suggest that a defect in arteriovenous identity could be responsible for the formation of telangiectases in patients with HLT...
  5. ncbi sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 3:127-36. 2002
    ..These studies reveal a complex signaling cascade responsible for establishing arterial cell fate and suggest differential effects of Vegf on developing endothelial cells...
  6. pmc Semaphorin-PlexinD1 signaling limits angiogenic potential via the VEGF decoy receptor sFlt1
    Tomasz Zygmunt
    Department of Cell Biology, Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Dev Cell 21:301-14. 2011
    ..Hence, Sema-PlxnD1 signaling regulates distinct but related aspects of angiogenesis: the spatial allocation of angiogenic capacity within a primary vessel and sprout guidance...
  7. ncbi fused-somites-like mutants exhibit defects in trunk vessel patterning
    Kenna M Shaw
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Dyn 235:1753-60. 2006
    ..Somitic expression of known vascular guidance factors efnb2a, sema3a1, and sema3a2 is aberrantly patterned in fss and bea mutants, suggesting that the vascular phenotype is ..
  8. pmc Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates
    Chang Z Chun
    Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Blood 113:1192-9. 2009
    ..The snrk-1 gene acts downstream or parallel to notch and upstream of gridlock during artery-vein specification, and the human gene compensates for zebrafish snrk-1 knockdown, suggesting evolutionary conservation of function...
  9. ncbi EphA4 is required for cell adhesion and rhombomere-boundary formation in the zebrafish
    Julie E Cooke
    Howard Hughes Medical Institute and Division of Basic Science, Fred Hutchinson Cancer Research Center B2 152, 1100 Fairview Avenue N, P O Box 19024, Seattle, Washington 98109, USA
    Curr Biol 15:536-42. 2005
    ....
  10. ncbi Eph/Ephrin signaling regulates the mesenchymal-to-epithelial transition of the paraxial mesoderm during somite morphogenesis
    Arantza Barrios
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    Curr Biol 13:1571-82. 2003
    ..These observations suggest a role for Eph/Ephrin signaling in the regulation of somite epithelialization...
  11. doi Gdf6a is required for the initiation of dorsal-ventral retinal patterning and lens development
    Curtis R French
    Department of Biological Sciences, University of Alberta, CW405, Biological Sciences Bldg, Edmonton T6G 2E9, Canada
    Dev Biol 333:37-47. 2009
    ..Taken together, these data indicate that Gdf6a initiates dorsal retinal patterning independent of Bmp4, and regulates lens differentiation...
  12. ncbi belladonna/(Ihx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain
    Anandita Seth
    Biology Department, University of Massachusetts, Amherst, MA 01003 9297, USA
    Development 133:725-35. 2006
    ..Our analysis reveals new roles for Ihx2 in midline axon guidance, forebrain patterning and eye morphogenesis...
  13. pmc Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate
    Charles Williams
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37203, USA
    Dev Biol 341:196-204. 2010
    ..Collectively, these studies suggest that arterial endothelial cells are specified and formed via repressing venous cell fate at the lateral plate mesoderm by Hh signaling during vasculogenesis...
  14. ncbi Endothelial signalling by the Notch ligand Delta-like 4 restricts angiogenesis
    Jonathan D Leslie
    Vertebrate Development Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 134:839-44. 2007
    ..Thus, Dll4-Notch signalling acts as an angiogenic ;off' switch by making endothelial cells unresponsive to Vegf...
  15. ncbi Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos
    Martin Gering
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, United Kingdom
    Dev Cell 8:389-400. 2005
    ..Furthermore, HSC and DA formation also share Vegf and Notch requirements, which further distinguishes them from primitive hematopoiesis and underlines their close relationship during vertebrate development...
  16. ncbi Boundary formation in the hindbrain: Eph only it were simple
    Julie E Cooke
    Howard Hughes Medical Institute, Division of Basic Sciences, Fred Hutchinson Cancer Research Center B2 152, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Trends Neurosci 25:260-7. 2002
    ..We discuss the contributions of two mechanisms -- cell sorting and plasticity -- to the formation of rhombomere boundaries...
  17. pmc An essential role for Radar (Gdf6a) in inducing dorsal fate in the zebrafish retina
    Nathan J Gosse
    Department of Physiology, Program in Developmental Biology, Genetics, University of California, 1550 Fourth Street, San Francisco, CA 94158 2722, USA
    Proc Natl Acad Sci U S A 106:2236-41. 2009
    ..We conclude that Radar is near the top of a signaling cascade that establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map...
  18. pmc A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development
    L D Covassin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, Room 617, 364 Plantation Street, Worcester, MA 01605, USA
    Dev Biol 329:212-26. 2009
    ..Together our genetic analyses suggest that Vegf/Plcg1 signaling acts at multiple time points and in different signaling contexts to mediate distinct aspects of artery development...
  19. ncbi Semaphorin-plexin signaling guides patterning of the developing vasculature
    Jesus Torres-Vazquez
    Laboratory of Molecular Genetics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 7:117-23. 2004
    ..These results reveal the fundamental conservation of repulsive patterning mechanisms between axonal migration in the central nervous system and vascular endothelium during angiogenesis...
  20. ncbi Dissection of angiogenic signaling in zebrafish using a chemical genetic approach
    Joanne Chan
    Department of Cancer Biology, Dana Farber Cancer Institute and the Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Cancer Cell 1:257-67. 2002
    ..This approach allowed us to examine the effects of blood flow and the role of endothelial signals in organogenesis...
  21. pmc phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development
    Nathan D Lawson
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:1346-51. 2003
    ..Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system...
  22. pmc Artery/vein specification is governed by opposing phosphatidylinositol-3 kinase and MAP kinase/ERK signaling
    Charles C Hong
    Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 16:1366-72. 2006
    ..In summary, chemical genetic analysis has uncovered unanticipated opposing roles of PI3K and ERK in artery/vein specification...
  23. pmc Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish
    L D Covassin
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 103:6554-9. 2006
    ....
  24. pmc EphA4 and EfnB2a maintain rhombomere coherence by independently regulating intercalation of progenitor cells in the zebrafish neural keel
    Hilary A Kemp
    Howard Hughes Medical Institute and Division of Basic Science, Fred Hutchinson Cancer Research Center, B2 152, 1100 Fairview Ave N, Seattle, WA 98109, P O Box 19024, USA
    Dev Biol 327:313-26. 2009
    ..The Eph receptor tyrosine kinase EphA4 and the membrane-bound Ephrin (Efn) ligand EfnB2a, which are expressed in complementary segments in the early hindbrain, are required for rhombomere boundary ..
  25. pmc Cooperative function of deltaC and her7 in anterior segment formation
    Andrew C Oates
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Dev Biol 280:133-49. 2005
    ..Thus, anterior segmentation requires the functions of both her and delta family members in a parallel manner, suggesting that the segmentation oscillator operates in paraxial mesoderm along the entire vertebrate axis...
  26. ncbi In vivo cell sorting in complementary segmental domains mediated by Eph receptors and ephrins
    Q Xu
    Division of Developmental Neurobiology, National Institute for Medical Research, London, UK
    Nature 399:267-71. 1999
    ..These data implicate Eph receptors and ephrins in the segmental restriction of cell intermingling...
  27. pmc Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs
    Yong Zhang
    Blood Cell Development and Cancer Keystone, Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Dev Cell 24:411-25. 2013
    ..Accordingly, circumstances that tip the balance of these paralogs in favor of Rpl22 (e.g., Rpl22l1 knockdown or Rpl22 overexpression) result in repression of Smad1 and blockade of HSC emergence...
  28. pmc Role of synectin in lymphatic development in zebrafish and frogs
    Karlien Hermans
    Vesalius Research Center, Vlaams Iustituut voor Biotechnologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49, Leuven, Belgium
    Blood 116:3356-66. 2010
    ..These findings reveal a novel role for synectin in lymphatic development...
  29. pmc Meis1 specifies positional information in the retina and tectum to organize the zebrafish visual system
    Timothy Erickson
    Department of Biological Sciences, University of Alberta, CW405, Biological Sciences Bldg, Edmonton T6G 2E9, Canada
    Neural Dev 5:22. 2010
    ..Meis1 is a TALE-class homeodomain transcription factor known to specify anterior-posterior identity in the hindbrain, but its role in visual system patterning has not been investigated...
  30. ncbi Molecular distinction between arteries and veins
    Jesus Torres-Vazquez
    Laboratory of Molecular Genetics, NICHD, NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
    Cell Tissue Res 314:43-59. 2003
    ..Here we review some of the molecular mechanisms involved in this process...
  31. doi A blood flow-dependent klf2a-NO signaling cascade is required for stabilization of hematopoietic stem cell programming in zebrafish embryos
    Lu Wang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Key Laboratory of Stem Cell Development, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Blood 118:4102-10. 2011
    ..Taken together, we have demonstrated that blood flow is essential for HSC development and is mediated by a klf2a-NO signaling cascade in zebrafish...
  32. pmc Role of Chd7 in zebrafish: a model for CHARGE syndrome
    Shunmoogum A Patten
    Sainte Justine Hospital Research Center, Montreal, Quebec, Canada
    PLoS ONE 7:e31650. 2012
    ..Chd7 knockdown also results in a loss of proper segment polarity illustrated by flawed efnb2a and ttna expression, which is associated with later vascular segmentation defects...
  33. pmc Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish
    Renee Kruse-Bend
    Department of Neurobiology and Anatomy, 20 North 1900 East, Room 401 MREB, University of Utah, Salt Lake City, UT 84132, USA
    Dev Biol 371:57-65. 2012
    ..We find that bmp2b is involved in dorsal retina initiation, acting upstream of gdf6a. Together, this work has identified the nature and source of extraocular signals required to pattern the dorsal retina...
  34. pmc Vascular endothelial growth factor signaling regulates the segregation of artery and vein via ERK activity during vascular development
    Se Hee Kim
    McAllister Heart Institute, Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Biochem Biophys Res Commun 430:1212-6. 2013
    ..Taken together, our data show that segregation of axial vessels requires the function of Vegf-A signaling, and Erk may function as the major downstream effector in this process...
  35. doi Functional characterization of Klippel-Trenaunay syndrome gene AGGF1 identifies a novel angiogenic signaling pathway for specification of vein differentiation and angiogenesis during embryogenesis
    Di Chen
    Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Wuhan, PR China
    Hum Mol Genet 22:963-76. 2013
    ..We propose that increased AGGF1 expression leads to increased vein differentiation by inducing activation of AKT signaling, resulting in VMs s in KTS patients...
  36. doi Segmental assembly of fibronectin matrix requires rap1b and integrin α5
    Simone Lackner
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Dev Dyn 242:122-31. 2013
    ..The direct activator of Integrin α5 during this process is unknown. One candidate is Rap1b, a small monomeric GTPase implicated in Integrin activation in the immune system...
  37. pmc The RAS guanyl nucleotide-releasing protein RasGRP1 is involved in lymphatic development in zebrafish
    Heng Huang
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China
    J Biol Chem 288:2355-64. 2013
    ..Taken together, this finding demonstrates a critical role for RasGRP1 in lymphatic development in zebrafish...
  38. pmc The transcriptional landscape of hematopoietic stem cell ontogeny
    Shannon McKinney-Freeman
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Cell Stem Cell 11:701-14. 2012
    ..Our analysis and web resource will enhance efforts to identify regulators of HSC ontogeny and facilitate the engineering of hematopoietic specification...
  39. doi Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling
    Dörthe Jülich
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Development 136:2913-21. 2009
    ..Within the tissue, interplay between Eph/Ephrin signaling, ligand-independent Integrin clustering and reciprocal Integrin inhibition restricts de novo ECM production to somite boundaries...
  40. ncbi Semaphorin3D guides retinal axons along the dorsoventral axis of the tectum
    Yan Liu
    Department of Zoology and Anatomy, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Neurosci 24:310-8. 2004
    ..These results suggest that Sema3D in the ventral tectum normally acts to inhibit ventral RGCs from extending into ventral tectum, ensuring their correct innervation of dorsal tectum...
  41. doi Hedgehog signaling via angiopoietin1 is required for developmental vascular stability
    Ryan E Lamont
    Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr NW, Calgary, AB, Canada T2N 4N1
    Mech Dev 127:159-68. 2010
    ..This is the first direct in vivo link between hedgehog signaling and the induction of vascular stability by recruitment of perivascular mural cells through angiopoietin signaling...
  42. pmc A genetic screen in zebrafish defines a hierarchical network of pathways required for hematopoietic stem cell emergence
    CAROLINE E BURNS
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 113:5776-82. 2009
    ..Together, our results establish a hierarchy of signaling programs required and sufficient for HSC emergence in the AGM...
  43. doi TRPC1 is essential for in vivo angiogenesis in zebrafish
    Peng chun Yu
    Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Circ Res 106:1221-32. 2010
    ..Activation of transient receptor potential type C channels (TRPCs) has recently been shown to underlie chemotropic guidance of neural axons. It is thus of interest to examine whether TRPCs are also involved in vascular development...
  44. ncbi Anteroposterior patterning is required within segments for somite boundary formation in developing zebrafish
    L Durbin
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK
    Development 127:1703-13. 2000
    ....
  45. doi Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis
    Yingdi Wang
    Vascular Development Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    Nature 465:483-6. 2010
    ..Our results show that full VEGFR3 signalling is coupled to receptor internalization. Ephrin-B2 is a key regulator of this process and thereby controls angiogenic and lymphangiogenic growth...
  46. doi Hey2 acts upstream of Notch in hematopoietic stem cell specification in zebrafish embryos
    Jonathan M Rowlinson
    Institute of Genetics, School of Biology, University of Nottingham, Queen s Medical Centre, Nottingham, UK
    Blood 116:2046-56. 2010
    ..These results establish an essential role for Hey2 upstream of Notch in HSC formation...
  47. pmc nev (cyfip2) is required for retinal lamination and axon guidance in the zebrafish retinotectal system
    Andrew J Pittman
    Program in Neuroscience, Department of Neurobiology and Anatomy, and Brain Institute, University of Utah Medical Center, Salt Lake City, UT 84132, USA
    Dev Biol 344:784-94. 2010
    ....
  48. pmc Mtmr8 is essential for vasculature development in zebrafish embryos
    Jie Mei
    State Key Laboratory of Freshwater Ecology and Biotechnology, Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan 430072, China
    BMC Dev Biol 10:96. 2010
    ..Here, we attempt to explore the function of Mtmr8 in vasculature development parallel to its function in muscle development...
  49. doi Zebrafish notch signalling pathway mutants exhibit trunk vessel patterning anomalies that are secondary to somite misregulation
    Christina Therapontos
    NHLI, Faculty of Medicine, Imperial College London, London, United Kingdom
    Dev Dyn 239:2761-8. 2010
    ..Ectopic filopodia are not due to loss of dll4. Somite expression of known vascular guidance cues, efnb2, sema3a2, and plexinD1 are disrupted, suggesting that the ISV vascular phenotype is due to disruption of these cues...
  50. pmc Zebrafish mll gene is essential for hematopoiesis
    Xiaoyang Wan
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
    J Biol Chem 286:33345-57. 2011
    ..Taken together, these findings demonstrate that zebrafish mll plays essential roles in hematopoiesis and that gadd45αa may serve as a potential downstream target for mediating the function of mll in hematopoiesis...
  51. ncbi Eph receptors and ephrins restrict cell intermingling and communication
    G Mellitzer
    Division of Developmental Neurobiology, National Institute for Medical Research, London, UK
    Nature 400:77-81. 1999
    ..These results reveal that Eph receptors and ephrins regulate two aspects of cell behaviour that can stabilize a distinct identity of adjacent cell populations...
  52. pmc Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish
    Cong Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 138:4555-64. 2011
    ..This work provides a resource to allow targeted germline gene inactivation in zebrafish and highlights the benefit of a definitive reverse genetic strategy to reveal gene function...
  53. pmc Mutation in utp15 disrupts vascular patterning in a p53-dependent manner in zebrafish embryos
    Kevin Mouillesseaux
    Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e25013. 2011
    ..Using the zebrafish as a model organism, we sought to identify genes that influence normal vascular patterning...
  54. ncbi Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation
    Randall T Peterson
    Nat Biotechnol 22:595-9. 2004
    ..Thus, organism-based screens allow the discovery of small molecules that ameliorate complex dysmorphic syndromes even without targeting the affected gene directly...
  55. pmc Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina
    Jeffrey M Gross
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 102:4371-6. 2005
    ..Combined, these observations suggest that the cellular mechanisms regulating neuronal differentiation within the retina are asymmetric about the dorsal/ventral axis and that Tbx2b mediates this process within the dorsal retina...
  56. pmc The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis
    Fatma O Kok
    Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, New York 11794, USA
    Dev Biol 307:214-26. 2007
    ..However, additional Spt6 mutant phenotypes are likely caused by vital functions of Spt6 in other pathways...
  57. pmc A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos
    Suk Won Jin
    Department of Biochemistry and Biophysics, Genetics and Human Genetics, and Cardiovascular Research Institute, University of California San Francisco, CA 94158, USA
    Dev Biol 307:29-42. 2007
    ..The analysis of the newly defined loci should lead to a greater understanding of vascular development and possibly provide new drug targets to treat the numerous pathologies associated with dysregulated blood vessel growth...
  58. ncbi Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish
    Tomomi Sato
    Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, Wako, Saitama 351 0198, Japan
    J Neurosci 27:5271-9. 2007
    ..These results may provide a neuroanatomical and molecular basis for the motor command map in the tectum...
  59. ncbi Chemokine signaling guides axons within the retina in zebrafish
    Qin Li
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Neurosci 25:1711-7. 2005
    ..These data suggest that chemokine signaling is both necessary and sufficient for directing retinal growth cones within the retina...
  60. ncbi Differentiation of arterial and venous endothelial cells and vascular morphogenesis
    Masanori Hirashima
    The Sakaguchi Laboratory of Developmental Biology, School of Medicine, Keio University, Tokyo, Japan
    Endothelium 13:137-45. 2006
    ..These insights indicate that the balance of these genetic factors and modification by epigenetic factors such as hemodynamics and oxygen tension are important for proper endothelial cell identities in vascular morphogenesis...
  61. ncbi EphrinB2a in the zebrafish retinotectal system
    Mahendra Wagle
    Developmental Neurobiology, Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604
    J Neurobiol 59:57-65. 2004
    ..In vitro, zebrafish RGC axons are repelled by stripes of purified ephrinB2a. It is proposed that ephrinB2a may signal a subpopulation of RGC axons that they have reached their target neurons in the tectum...
  62. pmc Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish
    Peter E Bayliss
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nat Chem Biol 2:265-73. 2006
    ..This study illustrates the utility of the adult zebrafish as a new model system for receptor signaling and chemical biology...
  63. ncbi Ethanol disrupts the formation of hypochord and dorsal aorta during the development of embryonic zebrafish
    Linxi Qian
    Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School of Fudan University, Shanghai 200032, China
    Sci China C Life Sci 48:608-15. 2005
    ..These results provide important clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus...
  64. ncbi Cyclooxygenase-1 signaling is required for vascular tube formation during development
    Yong I Cha
    Department of Medicine, Cell and Developmental Biology and Cancer Biology, Vanderbilt University Medical Center and Vanderbilt Ingram Cancer Center, Vanderbilt University, 694 Preston Research Building, 2300 Pierce Ave, Nashville, TN 37232, USA
    Dev Biol 282:274-83. 2005
    ..Together, we provide the first evidence that COX-1 signaling is required for development of posterior mesoderm organs, specifically in the vascular tube formation and posterior nephric duct development...
  65. ncbi Hedgehog regulated Slit expression determines commissure and glial cell position in the zebrafish forebrain
    Michael J F Barresi
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Development 132:3643-56. 2005
    ..This analysis shows that Hh signaling helps to pattern the expression of Slit guidance molecules that then help to regulate glial cell position and axon guidance across the midline of the forebrain...
  66. pmc Hematopoietic stem cell fate is established by the Notch-Runx pathway
    Caroline Erter Burns
    Stem Cell Program and Division of Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:2331-42. 2005
    ..These data define the Notch-Runx pathway as critical for the developmental specification of HSC fate and the subsequent homeostasis of HSC number, thus providing a mechanism for amplifying stem cells in vivo...
  67. ncbi Retinoic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish
    Yumi Emoto
    Department of Biological Information, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Dev Biol 278:415-27. 2005
    ..We propose a model in which Cyp26a1 attenuates RA signaling in the prospective rostral spinal cord to limit the expression of hox genes and to determine the hindbrain-spinal cord boundary...
  68. ncbi Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc)
    Jasmine D'Souza
    Developmental Neurobiology Group, Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Rep of Singapore
    Development 132:247-56. 2005
    ..These data identify a mediator of signal transduction in retinal growth cones, which is required for topographic map formation...
  69. ncbi Redundant roles for sox7 and sox18 in arteriovenous specification in zebrafish
    Robert Herpers
    Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Circ Res 102:12-5. 2008
    ..Our study identifies members of the Sox family as key factors in specifying arteriovenous identity and will help to better understand hypotrichosis-lymphedema-telangiectasia and other diseases...
  70. doi Expression screening and annotation of a zebrafish myoblast cDNA library
    Sarah Baxendale
    MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, UK
    Gene Expr Patterns 9:73-82. 2009
    ....
  71. ncbi vhnf1 integrates global RA patterning and local FGF signals to direct posterior hindbrain development in zebrafish
    Rafael E Hernandez
    Howard Hughes Medical Institute and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109, USA
    Development 131:4511-20. 2004
    ..The different requirements for vhnf1 and val to repress hoxb1a and ephrin-B2a, respectively, demonstrate that not all aspects of an individual rhombomere's identity are regulated coordinately...
  72. doi meis1 regulates the development of endothelial cells in zebrafish
    Ken ichi Minehata
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 374:647-52. 2008
    ..Thus, these results implicate that meis1 is a novel regulator involved in endothelial cell development, presumably affecting the vegf signaling pathway...
  73. doi ccm1 cell autonomously regulates endothelial cellular morphogenesis and vascular tubulogenesis in zebrafish
    Benjamin M Hogan
    Hubrecht Institute KNAW and University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Hum Mol Genet 17:2424-32. 2008
    ..Finally, we show that ccm1 function is cell autonomous, suggesting that it is endothelial cellular morphogenesis that is regulated by CCM proteins during development and pathogenesis...
  74. doi The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development
    Annette Schenck
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 133:486-97. 2008
    ..We propose that partitioning of Akt and selected effectors onto endosomal compartments represents a key mechanism contributing to the specificity of signal transduction in vertebrate development...
  75. doi Calcitonin receptor-like receptor guides arterial differentiation in zebrafish
    Stefania Nicoli
    Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    Blood 111:4965-72. 2008
    ..These data demonstrate that crlr plays a nonredundant role in arterial differentiation, representing a novel element of the sonic hedgehog-vegf-notch signaling cascade that controls arterial/venous fate...
  76. doi Zebrafish Sox7 and Sox18 function together to control arterial-venous identity
    Helene Pendeville
    GIGA Research Unité de Biologie Moleculaire et Génie Génétique, Tour B34, Universite de Liege, B 4000 Sart Tilman, Belgium
    Dev Biol 317:405-16. 2008
    ..The striking similarities between the phenotype of Sox7/Sox18 morphants and Gridlock mutants strongly suggest that Sox7 and Sox18 control arterial-venous identity by regulating Gridlock expression...
  77. ncbi Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation
    Steven M Pollard
    Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Dev Biol 289:64-76. 2006
    ....
  78. doi The receptor protein-tyrosine phosphatase, Dep1, acts in arterial/venous cell fate decisions in zebrafish development
    Fiona Rodriguez
    Hubrecht Institute KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Dev Biol 324:122-30. 2008
    ..Our results suggest a model in which Dep1 acts upstream in a signaling pathway inhibiting PI3K, resulting in expression of Notch and Grl, thus regulating arterial specification in development...
  79. pmc Hematopoietic stem cell development is dependent on blood flow
    Trista E North
    Stem Cell Program and Hematology Oncology, Children s Hospital, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 137:736-48. 2009
    ..This work links blood flow to AGM hematopoiesis and identifies NO as a conserved downstream regulator of HSC development...
  80. pmc Combinatorial function of ETS transcription factors in the developing vasculature
    Van N Pham
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 303:772-83. 2007
    ..Our results demonstrate that combinatorial ETS factor function is essential for early endothelial specification and differentiation...
  81. ncbi Integrinalpha5-dependent fibronectin accumulation for maintenance of somite boundaries in zebrafish embryos
    Sumito Koshida
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444 8787, Japan
    Dev Cell 8:587-98. 2005
    ....
  82. ncbi Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants
    Isabelle Foucher
    MRC Centre for Developmental Neurobiology, New Hunt s House, King s College London, London SE1 9RT, UK
    Development 133:1891-900. 2006
    ..This maintenance is enough to allow cerebellar differentiation...
  83. pmc Churchill and Sip1a repress fibroblast growth factor signaling during zebrafish somitogenesis
    Fatma O Kok
    Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, New York, USA
    Dev Dyn 239:548-58. 2010
    ..Finally, we found that blocking FGF8 restores somite morphology in ChCh and Sip1a compromised embryos. These results demonstrate a novel role for ChCh and Sip1a in repression of FGF activity...
  84. ncbi Dynamic and sequential patterning of the zebrafish posterior hindbrain by retinoic acid
    Lisa Maves
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
    Dev Biol 285:593-605. 2005
    ..Our results support a new model of dynamic RA action in the hindbrain, in which a temporally increasing source of RA is required to sequentially initiate progressively more posterior rhombomere identities...
  85. ncbi Selective regulation of arterial branching morphogenesis by synectin
    Thomas W Chittenden
    Angiogenesis Research Center, Section of Cardiology, Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Dev Cell 10:783-95. 2006
    ..We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process...
  86. doi A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3
    Aline Stedman
    CNRS UMR7622, Laboratoire de Biologie du Developpement, Universite Pierre et Marie Curie, Bâtiment C 7ème étage, Boite 24, 9 quai Saint Bernard, 75005 Paris, France
    Dev Biol 327:566-77. 2009
    ..Together, our data point to an essential function of Iroquois transcription factors in krox20 activation and, more generally, in anterior hindbrain specification...

Research Grants22

  1. Role of Plcg1 in Vegf signaling
    Nathan Lawson; Fiscal Year: 2009
    ..Since these signaling mechanisms are evolutionary conserved, the molecules we identify in this proposal represent possible targets for clinical manipulation of pathological blood vessel formation. ..
  2. Role of Notch in artery development
    Nathan D Lawson; Fiscal Year: 2010
    ..The studies in this proposal will address how blood vessel identities are determined during development. ..
  3. Endothelial lineage specification and differentiation in vertebrate embryos
    Suk Won Jin; Fiscal Year: 2009
    ..Furthermore, it will also help us to understand the etiology of many vascular diseases and design a better way of using pluripotent progenitors for the therapeutic purposes. ..
  4. Using zinc finger nucleases to manipulate the zebrafish genome
    Scot A Wolfe; Fiscal Year: 2010
    ..We will apply this technology in zebrafish to better understand the processes involved in heart regeneration and blood vessel formation. ..
  5. Endothelial lineage specification and differentiation in vertebrate embryos
    Suk Won Jin; Fiscal Year: 2010
    ..Furthermore, it will also help us to understand the etiology of many vascular diseases and design a better way of using pluripotent progenitors for the therapeutic purposes. ..
  6. Reverse Genetic Screening for Vascular Mutants Using Zinc Finger Nucleases
    Nathan D Lawson; Fiscal Year: 2010
    ..In this proposal, we will apply new technology to generate zebrafish knockouts in more than 30 genes to investigate their function during vascular development. ..
  7. Identifying Vegf target genes during artery development
    Nathan Lawson; Fiscal Year: 2005
    ....
  8. Transgenic screen for artery development mutants
    Nathan Lawson; Fiscal Year: 2007
    ..Together, these studies will allow identification of genes that are required for proper artery and vein development and will represent putative targets for therapeutic manipulation. ..
  9. Transcriptional Control of Endothelial Differentiation
    Nathan D Lawson; Fiscal Year: 2010
    ..However, little is known about how these differences arise. In this proposal, we will utilize the zebra fish as a model system to identify gene programs that govern blood vessel identity in the developing embryo. ..