Gene Symbol: cyp1c2
Description: cytochrome P450, family 1, subfamily C, polypeptide 2
Alias: cytochrome P450, family 1, subfamily C, polypeptide 2, cytochrome P450, family 1 member
Species: zebrafish

Top Publications

  1. Bugiak B, Weber L. Phenotypic anchoring of gene expression after developmental exposure to aryl hydrocarbon receptor ligands in zebrafish. Aquat Toxicol. 2010;99:423-37 pubmed publisher
    ..In contrast, TCDD transiently decreased CYP1C2 expression...
  2. Jönsson M, Orrego R, Woodin B, Goldstone J, Stegeman J. Basal and 3,3',4,4',5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish. Toxicol Appl Pharmacol. 2007;221:29-41 pubmed
    ..Here we cloned the paralogous CYP1C2, showing that the amino acid sequence is 78% identical to CYP1C1, and examined gene structure and expression of ..
  3. Jönsson M, Jenny M, Woodin B, Hahn M, Stegeman J. Role of AHR2 in the expression of novel cytochrome P450 1 family genes, cell cycle genes, and morphological defects in developing zebra fish exposed to 3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci. 2007;100:180-93 pubmed
    ..Basal and PCB126-induced expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2 was examined over time as well as in relation to cell cycle gene expression and morphological effects of PCB126 in ..
  4. Goldstone J, Goldstone H, Morrison A, Tarrant A, Kern S, Woodin B, et al. Cytochrome P450 1 genes in early deuterostomes (tunicates and sea urchins) and vertebrates (chicken and frog): origin and diversification of the CYP1 gene family. Mol Biol Evol. 2007;24:2619-31 pubmed publisher
    ..The CYP1 family has undergone multiple rounds of gene duplication followed by functional divergence, with at least one gene lost in mammals. This study provides new insight into the origin and evolution of CYP1 genes...
  5. Godard C, Goldstone J, Said M, Dickerson R, Woodin B, Stegeman J. The new vertebrate CYP1C family: cloning of new subfamily members and phylogenetic analysis. Biochem Biophys Res Commun. 2005;331:1016-24 pubmed
    ..These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE...
  6. Smith E, Iftikar F, Higgins S, Irshad A, Jandoc R, Lee M, et al. In vitro inhibition of cytochrome P450-mediated reactions by gemfibrozil, erythromycin, ciprofloxacin and fluoxetine in fish liver microsomes. Aquat Toxicol. 2012;109:259-66 pubmed publisher
    ..Expressed zebrafish CYP1 proteins (CYP1A, CYP1B1, CYP1C1 and CYP1C2) were assessed for inhibition with selected substrates...
  7. Schiwy S, Bräunig J, Alert H, Hollert H, Keiter S. A novel contact assay for testing aryl hydrocarbon receptor (AhR)-mediated toxicity of chemicals and whole sediments in zebrafish (Danio rerio) embryos. Environ Sci Pollut Res Int. 2015;22:16305-18 pubmed publisher
    ..The highest levels of all investigated cyp genes (cyp1a, cyp1b1, cyp1c1, and cyp1c2) were recorded after exposure to the sediment sample of the Vering canal...
  8. Blüthgen N, Meili N, Chew G, Odermatt A, Fent K. Accumulation and effects of the UV-filter octocrylene in adult and embryonic zebrafish (Danio rerio). Sci Total Environ. 2014;476-477:207-17 pubmed publisher
    ..Blood levels of 11-ketotestosterone were not altered. The transcriptomics data suggest that OC mainly affects transcription of genes related to developmental processes in the brain and liver as well as metabolic processes in the liver. ..
  9. Yang J, Zhu J, Chan K. BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells. Toxicol Appl Pharmacol. 2016;305:203-215 pubmed publisher
    ..CYP1A induction derived by BDE-99 warrants further risk assessment as the humans, wildlife and environment are exposed to a complex mixture including dioxin-like compounds and carcinogenic compounds. ..

More Information


  1. Scornaienchi M, Thornton C, Willett K, Wilson J. Functional differences in the cytochrome P450 1 family enzymes from zebrafish (Danio rerio) using heterologously expressed proteins. Arch Biochem Biophys. 2010;502:17-22 pubmed publisher
    ..We determined the catalytic activity of zebrafish CYP1A, CYP1B1, CYP1C1, CYP1C2, and CYP1D1 proteins using 11 fluorometric substrates and benzo[a]pyrene (BaP)...
  2. Zhou Z, Yang J, Chan K. Toxic effects of triclosan on a zebrafish (Danio rerio) liver cell line, ZFL. Aquat Toxicol. 2017;191:175-188 pubmed publisher
    ..The impairment of Cyp1a mRNA expression could be due to stimulation by other stressors such as oxidative stress, warranting further investigation into the underlying mechanism in zebrafish. ..
  3. Yang Y, Ji D, Huang X, Zhang J, Liu J. Effects of metals on enantioselective toxicity and biotransformation of cis-bifenthrin in zebrafish. Environ Toxicol Chem. 2017;36:2139-2146 pubmed publisher
    ..These results suggest that the influence of coexistent metals should be considered in the ecological risk assessment of chiral pyrethroids in aquatic environments. Environ Toxicol Chem 2017;36:2139-2146. © 2017 SETAC. ..
  4. Kubota A, Stegeman J, Woodin B, Iwanaga T, Harano R, Peterson R, et al. Role of zebrafish cytochrome P450 CYP1C genes in the reduced mesencephalic vein blood flow caused by activation of AHR2. Toxicol Appl Pharmacol. 2011;253:244-52 pubmed publisher
    ..The CYP1C subfamily was recently discovered in fish and includes the paralogues CYP1C1 and CYP1C2, both of which are induced via AHR2 in zebrafish embryos...
  5. J nsson M, Woodin B, Stegeman J, Brunstr m B. Cytochrome p450 1 genes in birds: evolutionary relationships and transcription profiles in chicken and Japanese quail embryos. PLoS ONE. 2011;6:e28257 pubmed publisher
    ..Determining CYP1 catalytic functions in different species should indicate the evolving roles of these duplicated genes in physiological and toxicological processes...
  6. Goodale B, La Du J, Bisson W, Janszen D, Waters K, Tanguay R. AHR2 mutant reveals functional diversity of aryl hydrocarbon receptors in zebrafish. PLoS ONE. 2012;7:e29346 pubmed publisher
  7. Chang C, Chung H, Su H, Tseng H, Tzou W, Hu C. Regulation of zebrafish CYP3A65 transcription by AHR2. Toxicol Appl Pharmacol. 2013;270:174-84 pubmed publisher
    ..It may also reflect the different environments that terrestrial and aquatic organisms encounter. ..
  8. Verbueken E, Alsop D, Saad M, Pype C, Van Peer E, Casteleyn C, et al. In Vitro Biotransformation of Two Human CYP3A Probe Substrates and Their Inhibition during Early Zebrafish Development. Int J Mol Sci. 2017;18: pubmed publisher
    ..Furthermore, Luciferin-IPA was not metabolized by the zebrafish. In conclusion, the capacity of intrinsic biotransformation in zebrafish embryos appears to be lacking during a major part of organogenesis. ..
  9. Knecht A, Goodale B, Truong L, Simonich M, Swanson A, Matzke M, et al. Comparative developmental toxicity of environmentally relevant oxygenated PAHs. Toxicol Appl Pharmacol. 2013;271:266-75 pubmed publisher
    ..Comprehensive in vivo characterization of 38 structurally diverse OPAHs indicated differential AHR dependency and a prominent role for oxidative stress in the toxicity mechanisms...
  10. Stegeman J, Behrendt L, Woodin B, Kubota A, Lemaire B, Pompon D, et al. Functional characterization of zebrafish cytochrome P450 1 family proteins expressed in yeast. Biochim Biophys Acta. 2015;1850:2340-52 pubmed publisher
    Zebrafish express five cytochrome P450 1 genes: CYP1A, CYP1B1, CYP1C1, CYP1C2, inducible by aryl hydrocarbon receptor agonists, and CYP1D1, a constitutively expressed CYP1A-like gene...
  11. Kühnert A, Vogs C, Seiwert B, Aulhorn S, Altenburger R, Hollert H, et al. Biotransformation in the zebrafish embryo -temporal gene transcription changes of cytochrome P450 enzymes and internal exposure dynamics of the AhR binding xenobiotic benz[a]anthracene. Environ Pollut. 2017;230:1-11 pubmed publisher
    ..Time dependent changes in cyp transcription (cyp1a, cyp1b1, cyp1c1 and cyp1c2) as well as concentration & time-dependent courses of BaA in the fish embryo and the exposure medium were ..
  12. Šrut M, Bourdineaud J, Štambuk A, Klobučar G. Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line. J Appl Toxicol. 2015;35:1381-9 pubmed publisher
  13. Bräunig J, Schiwy S, Broedel O, Müller Y, Frohme M, Hollert H, et al. Time-dependent expression and activity of cytochrome P450 1s in early life-stages of the zebrafish (Danio rerio). Environ Sci Pollut Res Int. 2015;22:16319-28 pubmed publisher
    ..In the present study, gene expression of cyp1a, cyp1b1, cyp1c1, cyp1c2, and ahr2 as well as EROD activity were investigated up to 120 h postfertilization (hpf) after exposure to either ..
  14. Truong L, Bugel S, Chlebowski A, Usenko C, Simonich M, Simonich S, et al. Optimizing multi-dimensional high throughput screening using zebrafish. Reprod Toxicol. 2016;65:139-147 pubmed publisher
    ..Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories. ..
  15. Goodale B, Tilton S, Corvi M, Wilson G, Janszen D, Anderson K, et al. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish. Toxicol Appl Pharmacol. 2013;272:656-70 pubmed publisher
    ..These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. ..
  16. Wincent E, Kubota A, Timme Laragy A, Jönsson M, Hahn M, Stegeman J. Biological effects of 6-formylindolo[3,2-b]carbazole (FICZ) in vivo are enhanced by loss of CYP1A function in an Ahr2-dependent manner. Biochem Pharmacol. 2016;110-111:117-29 pubmed publisher
  17. Harskamp J, Britz McKibbin P, Wilson J. Functional screening of cytochrome P450 activity and uncoupling by capillary electrophoresis. Anal Chem. 2012;84:862-6 pubmed publisher
    ..This work highlights the wide disparity in uncoupling induced by unproductive substrate binding among different CYP isoforms. ..
  18. Scornaienchi M, Thornton C, Willett K, Wilson J. Cytochrome P450-mediated 17beta-estradiol metabolism in zebrafish (Danio rerio). J Endocrinol. 2010;206:317-25 pubmed publisher
    ..We have cloned and heterologously expressed CYP1A, CYP1B1, CYP1C1, CYP1C2, CYP1D1, and CYP3A65 from zebrafish (Danio rerio) to determine the CYP-mediated metabolism of E(2) in a non-..
  19. Bugiak B, Weber L. Hepatic and vascular mRNA expression in adult zebrafish (Danio rerio) following exposure to benzo-a-pyrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Aquat Toxicol. 2009;95:299-306 pubmed publisher
    ..3 fold-increase, mean+/-SEM respectively). TCDD also increased CYP1A, CYP1B1, CYP1C1, CYP1C2, and COX-1 mRNA expression in mesenteric artery (121+/-23, 5+/-1, 28+/-6, 7+/-1, and 3+/-0.3, respectively)...
  20. Wincent E, Jönsson M, Bottai M, Lundstedt S, Dreij K. Aryl hydrocarbon receptor activation and developmental toxicity in zebrafish in response to soil extracts containing unsubstituted and oxygenated PAHs. Environ Sci Technol. 2015;49:3869-77 pubmed publisher
    ..In addition to Ahr activating potency, the profile of cytochrome P4501 inhibitors may also determine the toxic potency of the extracts. ..
  21. Yuan L, Lv B, Zha J, Wang Z, Wang W, Li W, et al. New cytochrome P450 1B1, 1C1, 2Aa, 2Y3, and 2K genes from Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression and response of rare minnow CYP1s and CYP2s mRNA exposed to the AHR agonist benzo[a]pyrene. Chemosphere. 2013;93:209-16 pubmed publisher
    ..The high responsiveness of CYP1 and 2 genes suggested Chinese rare minnow is feasible to screen and assess pollution with AHR agonist. ..
  22. Yang Y, Ye X, He B, Liu J. Cadmium potentiates toxicity of cypermethrin in zebrafish. Environ Toxicol Chem. 2016;35:435-45 pubmed publisher
    ..The authors' findings provide a deeper understanding of the mechanistic basis accounting for the joint toxicity of cypermethrin and Cd. ..
  23. Gao K, Brandt I, Goldstone J, Jönsson M. Cytochrome P450 1A, 1B, and 1C mRNA induction patterns in three-spined stickleback exposed to a transient and a persistent inducer. Comp Biochem Physiol C Toxicol Pharmacol. 2011;154:42-55 pubmed publisher
    ..The cDNAs of stickleback CYP1A, CYP1B1, CYP1C1, and CYP1C2 were cloned and their basal and induced expression patterns were determined in the brain, gill, liver and kidney...
  24. Behrendt L, Jönsson M, Goldstone J, Stegeman J. Induction of cytochrome P450 1 genes and stress response genes in developing zebrafish exposed to ultraviolet radiation. Aquat Toxicol. 2010;98:74-82 pubmed publisher
    ..93 W/m(2) (26.8 kJ/m(2)) significantly induced CYP1A and CYP1B1 expression, but other CYP1 genes (CYP1C1, CYP1C2 and CYP1D1) showed no significant increase...
  25. Jönsson M, Franks D, Woodin B, Jenny M, Garrick R, Behrendt L, et al. The tryptophan photoproduct 6-formylindolo[3,2-b]carbazole (FICZ) binds multiple AHRs and induces multiple CYP1 genes via AHR2 in zebrafish. Chem Biol Interact. 2009;181:447-54 pubmed publisher two zebrafish AHRs (AHR1B and AHR2) and its ability to induce zebrafish CYP1 genes (CYP1A, CYP1B1, CYP1C1, CYP1C2, and CYP1D1) in vivo...
  26. Jia Z, Liu D, Sheng C, Casida J, Wang C, Song P, et al. Acute toxicity, bioconcentration, elimination and antioxidant effects of fluralaner in zebrafish, Danio rerio. Environ Pollut. 2018;232:183-190 pubmed publisher
    ..Meanwhile, transcriptional expression of CYP1C2, CYP1D1, CYP11A were significantly down-regulated at 12 h exposed to 2.00 mg L-1 of fluralaner...