Gene Symbol: cyp1a
Description: cytochrome P450, family 1, subfamily A
Alias: cyp1a1, wu:fb63b04, zfCYP1A, zgc:109747, cytochrome P450 1A1, cytochrome P450, subfamily I, polypeptide 1
Species: zebrafish

Top Publications

  1. Andreasen E, Spitsbergen J, Tanguay R, Stegeman J, Heideman W, Peterson R. Tissue-specific expression of AHR2, ARNT2, and CYP1A in zebrafish embryos and larvae: effects of developmental stage and 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. Toxicol Sci. 2002;68:403-19 pubmed
    ..aryl hydrocarbon receptor nuclear translocator 2 (zfARNT2), and an AHR regulated gene, cytochrome P4501A (zfCYP1A), were assessed in larvae exposed to vehicle or TCDD (1.55 nM) from 3-4 h postfertilization (hpf)...
  2. Arukwe A, Nordtug T, Kortner T, Mortensen A, Brakstad O. Modulation of steroidogenesis and xenobiotic biotransformation responses in zebrafish (Danio rerio) exposed to water-soluble fraction of crude oil. Environ Res. 2008;107:362-70 pubmed publisher
    The induction of CYP enzyme activities, particularly CYP1A1, through the aryl hydrocarbon receptor (AhR) in most vertebrate species is among the most studied biochemical response to planar and aromatic organic contaminant exposure...
  3. Chung W, Sen A, Wang Buhler J, Yang Y, Lopez N, Merrill G, et al. cDNA-directed expression of a functional zebrafish CYP1A in yeast. Aquat Toxicol. 2004;70:111-21 pubmed
    A cytochrome P450 1A (CYP1A) cDNA was isolated from an adult zebrafish (Danio rerio) library. The 2580-bp clone (GenBank Accession No. AF210727) contained a 62-bp 5'-unstranslated region (UTR), 1557-bp coding region and 962-bp 3'-UTR...
  4. Antkiewicz D, Peterson R, Heideman W. Blocking expression of AHR2 and ARNT1 in zebrafish larvae protects against cardiac toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci. 2006;94:175-82 pubmed
    ..used morpholino oligonucleotide technology to specifically block the expression of zfAHR2, zfARNT1, zfARNT2, and zfCYP1A, and assessed the previously described effects of TCDD on heart morphology, size, and function in the developing ..
  5. Jones H, Panter G, Hutchinson T, Chipman J. Oxidative and conjugative xenobiotic metabolism in zebrafish larvae in vivo. Zebrafish. 2010;7:23-30 pubmed publisher
    ..mammalian cytochromes P450 (CYP) 2B6, CYP3A5, and UDP-glucuronosyl transferase (UGT) 1A1, as well as the zebrafish CYP1A was assessed across embryonic development...
  6. Planchart A, Mattingly C. 2,3,7,8-Tetrachlorodibenzo-p-dioxin upregulates FoxQ1b in zebrafish jaw primordium. Chem Res Toxicol. 2010;23:480-7 pubmed publisher
    ..The rate of TCDD-induced FoxQ1b expression was more rapid than that of Cyp1a, a known direct target of TCDD-activated Ahr...
  7. Incardona J, Carls M, Teraoka H, Sloan C, Collier T, Scholz N. Aryl hydrocarbon receptor-independent toxicity of weathered crude oil during fish development. Environ Health Perspect. 2005;113:1755-62 pubmed is widely held that PAH toxicity is linked to aryl hydrocarbon receptor (AhR) binding and cytochrome P450 1A (CYP1A) induction...
  8. Andreasen E, Mathew L, LOHR C, Hasson R, Tanguay R. Aryl hydrocarbon receptor activation impairs extracellular matrix remodeling during zebra fish fin regeneration. Toxicol Sci. 2007;95:215-26 pubmed
    ..Quantitative real-time PCR studies revealed that the aryl hydrocarbon pathway is active and that matrix-remodeling genes are expressed in the regenerate following TCDD exposure. ..
  9. Goldstone H, Stegeman J. Molecular mechanisms of 2,3,7,8-tetrachlorodibenzo-p-dioxin cardiovascular embryotoxicity. Drug Metab Rev. 2006;38:261-89 pubmed
    ..Gene expression profiling is elucidating additional pathways by which TCDD might act. We review our understanding of the mechanism of TCDD embryotoxicity at morphological and molecular levels. ..

More Information


  1. Voelker D, Vess C, Tillmann M, Nagel R, Otto G, Geisler R, et al. Differential gene expression as a toxicant-sensitive endpoint in zebrafish embryos and larvae. Aquat Toxicol. 2007;81:355-64 pubmed
    ..Six genes involved in biotransformation (cyp1a, ahr2), stress response (nfe212, maft, hmox1) and cell cycle control (fzr1) were significantly regulated...
  2. Incardona J, Day H, Collier T, Scholz N. Developmental toxicity of 4-ring polycyclic aromatic hydrocarbons in zebrafish is differentially dependent on AH receptor isoforms and hepatic cytochrome P4501A metabolism. Toxicol Appl Pharmacol. 2006;217:308-21 pubmed
    ..receptor (AHR), which controls a battery of genes involved in PAH metabolism, such as cytochrome P4501A (CYP1A) and (2) "nonpolar narcosis", in which tissue uptake is dependent solely on hydrophobicity and toxicity ..
  3. Carney S, Chen J, Burns C, Xiong K, Peterson R, Heideman W. Aryl hydrocarbon receptor activation produces heart-specific transcriptional and toxic responses in developing zebrafish. Mol Pharmacol. 2006;70:549-61 pubmed
    ..More than 70% of the transcripts in this heart-specific cluster promote cellular growth and proliferation. Thus, the developing heart stands out as being responsive to TCDD at both the level of toxicity and gene expression. ..
  4. Billiard S, Timme Laragy A, Wassenberg D, Cockman C, Di Giulio R. The role of the aryl hydrocarbon receptor pathway in mediating synergistic developmental toxicity of polycyclic aromatic hydrocarbons to zebrafish. Toxicol Sci. 2006;92:526-36 pubmed strong binding affinity for the aryl hydrocarbon receptor (AHR) and are potent inducers of cytochrome P4501A (CYP1A)...
  5. Mathew L, Andreasen E, Tanguay R. Aryl hydrocarbon receptor activation inhibits regenerative growth. Mol Pharmacol. 2006;69:257-65 pubmed
    ..With the plethora of molecular and genetic techniques that can be applied to larval-stage embryos, this in vivo regeneration system can be further exploited to understand cross-talk between AHR and other signaling pathways. ..
  6. Prasch A, Teraoka H, Carney S, Dong W, Hiraga T, Stegeman J, et al. Aryl hydrocarbon receptor 2 mediates 2,3,7,8-tetrachlorodibenzo-p-dioxin developmental toxicity in zebrafish. Toxicol Sci. 2003;76:138-50 pubmed
    ..Injection of embryos at the 1-2 cell stage with zfahr2-MO decreased TCDD-induced transcription of zfCYP1A mRNA until 96 h post fertilization (hpf), and immuno-histochemical detection of zfCYP1A protein in embryos at 72 ..
  7. Hawliczek A, Nota B, Cenijn P, Kamstra J, Pieterse B, Winter R, et al. Developmental toxicity and endocrine disrupting potency of 4-azapyrene, benzo[b]fluorene and retene in the zebrafish Danio rerio. Reprod Toxicol. 2012;33:213-23 pubmed publisher
    ..that for BBF and retene, drug metabolism pathways were induced, which were confirmed by subsequent studies of cyp1a gene expression...
  8. Dong W, Teraoka H, Yamazaki K, Tsukiyama S, Imani S, Imagawa T, et al. 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo: local circulation failure in the dorsal midbrain is associated with increased apoptosis. Toxicol Sci. 2002;69:191-201 pubmed
    ..of TCDD exposure concentrations that reduced blood flow and increased apoptosis in the midbrain greatly enhanced CYP1A mRNA expression and immunoreactivity at 50 hpf in endothelial cells of blood vessels including the mesencephalic ..
  9. Jönsson M, Jenny M, Woodin B, Hahn M, Stegeman J. Role of AHR2 in the expression of novel cytochrome P450 1 family genes, cell cycle genes, and morphological defects in developing zebra fish exposed to 3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci. 2007;100:180-93 pubmed
    ..e., PCNA and cyclin E) in zebra fish (Danio rerio) embryos. Basal and PCB126-induced expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2 was examined over time as well as in relation to cell cycle gene expression and ..
  10. Evans B, Karchner S, Franks D, Hahn M. Duplicate aryl hydrocarbon receptor repressor genes (ahrr1 and ahrr2) in the zebrafish Danio rerio: structure, function, evolution, and AHR-dependent regulation in vivo. Arch Biochem Biophys. 2005;441:151-67 pubmed
    ..and AHRR2 expression in a dose-dependent manner in ZF-L cells, with EC50 values similar to those for induction of CYP1A. Both AHRRs were expressed and induced by TCDD in zebrafish embryos...
  11. Yang L, Kemadjou J, Zinsmeister C, Bauer M, Legradi J, Muller F, et al. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 2007;8:R227 pubmed
    ..Our work provides proof-of-principle for the use of the zebrafish embryo as a toxicogenomic model and highlights its potential for systematic, large-scale analysis of the effects of chemicals on the developing vertebrate embryo. ..
  12. van Soest J, Stockhammer O, Ordas A, Bloemberg G, Spaink H, Meijer A. Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda. BMC Immunol. 2011;12:58 pubmed publisher
    ..In this study we used transcriptome profiling and quantitative RT-PCR to analyze the immune response induced by E. tarda immersion and injection...
  13. Carney S, Peterson R, Heideman W. 2,3,7,8-Tetrachlorodibenzo-p-dioxin activation of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator pathway causes developmental toxicity through a CYP1A-independent mechanism in zebrafish. Mol Pharmacol. 2004;66:512-21 pubmed
    ..Although the zfcyp1a-MO effectively prevented CYP1A up-regulation, it did not prevent the signs of developmental toxicity, including ..
  14. Wahl M, Lahni B, Guenther R, Kuch B, Yang L, Straehle U, et al. A technical mixture of 2,2',4,4'-tetrabromo diphenyl ether (BDE47) and brominated furans triggers aryl hydrocarbon receptor (AhR) mediated gene expression and toxicity. Chemosphere. 2008;73:209-15 pubmed publisher
    ..Initially, we measured cytochrome P450 1A1 (Cyp1A1) induction as a readout for AhR activation by BDE47...
  15. Zhen H, Wen M, Yang Y, Can Z, Hui G, Li X, et al. Toxic effects of HgCl2 on activities of SOD, AchE and relative expression of SOD, AChE, CYP1A1 of zebrafish. Ecotoxicology. 2014;23:1842-5 pubmed publisher
    ..Muscle RNA was also extracted for semi-quantitative RT-PCR determination of the expression of AChE, CYP1A1, Cu/Zn-SOD. It indicated that 96 h-LC50 of HgCl2 to zebrafish is 244.06 µg L(-1)...
  16. Olivares A, van Drooge B, Casado M, Prats E, Serra M, van der Ven L, et al. Developmental effects of aerosols and coal burning particles in zebrafish embryos. Environ Pollut. 2013;178:72-9 pubmed publisher
    ..This sample induced deformities in zebrafish embryos. We concluded that the combination of morphological and molecular assays may detect embryonic toxic effects that cannot be predicted from chemical analyses or single-cell bioassays. ..
  17. Wu Y, Jiang L, Zhou Z, Zheng M, Zhang J, Liang Y. CYP1A/regucalcin gene expression and edema formation in zebrafish embryos exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Bull Environ Contam Toxicol. 2008;80:482-6 pubmed publisher
    ..Mortality, heart rates, edema severity, CYP1A, and regucalcin gene expressions were investigated to study TCDD-induced toxicity in zebrafish during the early ..
  18. Lee J, Yoon H, Lee S. Benzo(a)pyrene-induced cytochrome p4501A expression of four freshwater fishes (Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus). Environ Toxicol Pharmacol. 2015;39:1041-50 pubmed publisher
    Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus are useful indicator species for CYP1A biomarker studies; however, comparative studies have not been performed...
  19. Lemaire B, Kubota A, O Meara C, Lamb D, Tanguay R, Goldstone J, et al. Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders. Toxicol Appl Pharmacol. 2016;296:73-84 pubmed publisher
    ..The expanded information on CYP20A1 brings us closer to "deorphanization", that is, identifying CYP20A1 functions and its roles in health and disease. ..
  20. Bestman J, Stackley K, Rahn J, Williamson T, Chan S. The cellular and molecular progression of mitochondrial dysfunction induced by 2,4-dinitrophenol in developing zebrafish embryos. Differentiation. 2015;89:51-69 pubmed publisher
  21. Yang Y, Ji D, Huang X, Zhang J, Liu J. Effects of metals on enantioselective toxicity and biotransformation of cis-bifenthrin in zebrafish. Environ Toxicol Chem. 2017;36:2139-2146 pubmed publisher
    ..These results suggest that the influence of coexistent metals should be considered in the ecological risk assessment of chiral pyrethroids in aquatic environments. Environ Toxicol Chem 2017;36:2139-2146. © 2017 SETAC. ..
  22. Goodale B, Tilton S, Corvi M, Wilson G, Janszen D, Anderson K, et al. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish. Toxicol Appl Pharmacol. 2013;272:656-70 pubmed publisher
    ..While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs...
  23. Mukhopadhyay D, Srivastava R, Chattopadhyay A. Sodium fluoride generates ROS and alters transcription of genes for xenobiotic metabolizing enzymes in adult zebrafish (Danio rerio) liver: expression pattern of Nrf2/Keap1 (INrf2). Toxicol Mech Methods. 2015;25:364-73 pubmed publisher
    ..The mRNA levels of genes for xenobiotic metabolizing enzymes (XMEs) like cytochrome P450 1A (Cyp1A), NADPH Q Oxidase 1 (Nqo1) and Heme Oxygenase 1 (Ho-1) increased along with nuclear factor (erythroid-derived 2)-..
  24. Baudiffier D, Hinfray N, Ravaud C, Creusot N, Chadili E, Porcher J, et al. Effect of in vivo chronic exposure to clotrimazole on zebrafish testis function. Environ Sci Pollut Res Int. 2013;20:2747-60 pubmed publisher
    ..Our study demonstrated that clotrimazole is able to affect testicular physiology and raised further concern about the impact of clotrimazole on reproduction. ..
  25. Incardona J, Swarts T, Edmunds R, Linbo T, Aquilina Beck A, Sloan C, et al. Exxon Valdez to Deepwater Horizon: comparable toxicity of both crude oils to fish early life stages. Aquat Toxicol. 2013;142-143:303-16 pubmed publisher
  26. Goldstone J, Jonsson M, Behrendt L, Woodin B, Jenny M, Nelson D, et al. Cytochrome P450 1D1: a novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD. Arch Biochem Biophys. 2009;482:7-16 pubmed publisher
    ..We identified a new CYP1, termed CYP1D1, in zebrafish. Phylogenetically, CYP1D1 is paralogous to CYP1A and the two share 45% amino acid identity and similar gene structure...
  27. Bugiak B, Weber L. Phenotypic anchoring of gene expression after developmental exposure to aryl hydrocarbon receptor ligands in zebrafish. Aquat Toxicol. 2010;99:423-37 pubmed publisher
    ..Exposure to TCDD, BaP and ANF alone all significantly increased CYP1A mRNA expression, while only TCDD consistently increased CYP1C1 expression...
  28. Blüthgen N, Zucchi S, Fent K. Effects of the UV filter benzophenone-3 (oxybenzone) at low concentrations in zebrafish (Danio rerio). Toxicol Appl Pharmacol. 2012;263:184-94 pubmed publisher
    ..Forthcoming studies should show whether this translates to additional physiological effects. ..
  29. Oh S, Ryu B, Kim H, Choi K, Chung K. Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus). Environ Toxicol. 2009;24:225-34 pubmed publisher
    We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino acid protein with a theoretical molecular weight of 59.02 kDa...
  30. Tang Y, Junaid M, Niu A, Deng S, Pei D. Diverse toxicological risks of PAHs in surface water with an impounding level of 175m in the Three Gorges Reservoir Area, China. Sci Total Environ. 2017;580:1085-1096 pubmed publisher
    ..Moreover, the transgenic zebrafish Tg(cyp1a:gfp) induced by PAHs also expressed stronger fluorescent signals in the middle and lower reaches...
  31. Verbueken E, Alsop D, Saad M, Pype C, Van Peer E, Casteleyn C, et al. In Vitro Biotransformation of Two Human CYP3A Probe Substrates and Their Inhibition during Early Zebrafish Development. Int J Mol Sci. 2017;18: pubmed publisher
    ..Furthermore, Luciferin-IPA was not metabolized by the zebrafish. In conclusion, the capacity of intrinsic biotransformation in zebrafish embryos appears to be lacking during a major part of organogenesis. ..
  32. Jönsson M, Orrego R, Woodin B, Goldstone J, Stegeman J. Basal and 3,3',4,4',5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish. Toxicol Appl Pharmacol. 2007;221:29-41 pubmed
    ..showing that the amino acid sequence is 78% identical to CYP1C1, and examined gene structure and expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2...
  33. Xu H, Li C, Suklai P, Zeng Q, Chong R, Gong Z. Differential sensitivities to dioxin-like compounds PCB 126 and PeCDF between Tg(cyp1a:gfp) transgenic medaka and zebrafish larvae. Chemosphere. 2018;192:24-30 pubmed publisher
    ..This study aimed at evaluating the differential sensitivities to DLCs in fish larvae. Here, larvae of Tg(cyp1a:gfp) medaka and Tg(cyp1a:gfp) zebrafish were tested with 2,3,7,8-Tetrachlorodibenzodioxin (TCDD), polychlorinated ..
  34. Sun Y, Zhang G, He Z, Wang Y, Cui J, Li Y. Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae. Int J Nanomedicine. 2016;11:905-18 pubmed publisher
    ..5, 6.25, or 1 mg/L. The expression of gstp1 and cyp1a was examined by quantitative reverse transcription polymerase chain reaction...
  35. Cunha V, Santos M, Moradas Ferreira P, Ferreira M. Simvastatin effects on detoxification mechanisms in Danio rerio embryos. Environ Sci Pollut Res Int. 2016;23:10615-29 pubmed publisher
    ..mRNA expression levels of abcb4, abcc1, abcc2, abcg2, cyp1a, cyp3a65, gst, sod, cat was evaluated...
  36. Bugel S, Bonventre J, Tanguay R. Comparative Developmental Toxicity of Flavonoids Using an Integrative Zebrafish System. Toxicol Sci. 2016;154:55-68 pubmed
    ..expression was comparatively evaluated for all compounds for effects on biomarker transcripts indicative of AHR (cyp1a) and ER (cyp19a1b, esr1, lhb, vtg) pathway bioactivity...
  37. Zhao Q, Shi F, Zhu L. Prometryn and humic acid induce Cytochrome P450 1A expression in Danio rerio (zebrafish). Ecotoxicol Environ Saf. 2017;135:40-47 pubmed publisher
    ..In contrast, recovery regularity of CYP 1A expression in gills was independent of the presence of HA. This result indicates different detoxification mechanisms for HA in liver and gills. ..
  38. McCurley A, Callard G. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Mol Biol. 2008;9:102 pubmed publisher
    ..different reference genes to measure suppression of cyp19a1b by an estrogen receptor antagonist and induction of cyp1a by an arylhydrocarbon receptor agonist, the direction and magnitude of effects with stable and unstable genes ..
  39. Zhang J, Sun T, Niu A, Tang Y, Deng S, Luo W, et al. Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish. Biomaterials. 2017;133:49-59 pubmed publisher
    ..Since the real-time polymerase chain reaction (RT-PCR) results showed that the expression of cyp1a was the highest expression in the selected genes and significantly up-regulated 8...
  40. Jönsson M, Franks D, Woodin B, Jenny M, Garrick R, Behrendt L, et al. The tryptophan photoproduct 6-formylindolo[3,2-b]carbazole (FICZ) binds multiple AHRs and induces multiple CYP1 genes via AHR2 in zebrafish. Chem Biol Interact. 2009;181:447-54 pubmed publisher
    ..the mammalian aryl hydrocarbon receptor (AHR), which it binds with high-affinity, inducing expression of cytochrome P450 1A1 (CYP1A1)...
  41. Garner L, Brown D, Di Giulio R. Knockdown of AHR1A but not AHR1B exacerbates PAH and PCB-126 toxicity in zebrafish (Danio rerio) embryos. Aquat Toxicol. 2013;142-143:336-46 pubmed publisher
    ..Knockdown of AHR1A did not impact the mRNA expression of CYP1A, CYP1B1, and CYP1C1 in exposed embryos, but it did result in increased CYP1 activity in exposed embryos...
  42. Timme Laragy A, Cockman C, Matson C, Di Giulio R. Synergistic induction of AHR regulated genes in developmental toxicity from co-exposure to two model PAHs in zebrafish. Aquat Toxicol. 2007;85:241-50 pubmed
    ..model, this toxicity is mediated by the aryl hydrocarbon receptor 2 (AHR2), and enhanced by inhibition of CYP1A activity...
  43. David R, Jones H, Panter G, Winter M, Hutchinson T, Kevin Chipman J. Interference with xenobiotic metabolic activity by the commonly used vehicle solvents dimethylsulfoxide and methanol in zebrafish (Danio rerio) larvae but not Daphnia magna. Chemosphere. 2012;88:912-7 pubmed publisher
  44. Singh R, Khatri P, Srivastava N, Jain S, Brahmachari V, Mukhopadhyay A, et al. Fluoride exposure abates pro-inflammatory response and induces in vivo apoptosis rendering zebrafish (Danio rerio) susceptible to bacterial infections. Fish Shellfish Immunol. 2017;63:314-321 pubmed publisher
    ..We observed significant elevation in the detoxification pathway gene cyp1a suggesting chronic exposure to non-lethal concentration of fluoride is indeed toxic to fish...
  45. Zhang H, Yao Y, Chen Y, Yue C, Chen J, Tong J, et al. Crosstalk between AhR and wnt/?-catenin signal pathways in the cardiac developmental toxicity of PM2.5 in zebrafish embryos. Toxicology. 2016;355-356:31-8 pubmed publisher
    ..We further found that EOM had increased the expression levels of AhR targeted genes (Cyp1a1, Cyp1b1 and Ahrra) and reduced the mRNA levels of ?-catenin targeted genes (axin2, nkx2.5 and sox9b)...
  46. Barranco A, Escudero L, Sanz Landaluze J, Rainieri S. Detection of exposure effects of mixtures of heavy polycyclic aromatic hydrocarbons in zebrafish embryos. J Appl Toxicol. 2017;37:253-264 pubmed publisher
    ..After 24 h of exposure, all PAHs, except for benzo[a]anthracene, acted as potent inducers of the gene cyp1a1. Benzo[k]fluoranthene was the major inducer; the effect caused by the mixture at the lower concentration tested (1..
  47. Hahn M, McArthur A, Karchner S, Franks D, Jenny M, Timme Laragy A, et al. The transcriptional response to oxidative stress during vertebrate development: effects of tert-butylhydroquinone and 2,3,7,8-tetrachlorodibenzo-p-dioxin. PLoS ONE. 2014;9:e113158 pubmed publisher
    ..tBHQ also induced gclc (2 dpf), but not sod1, nqo1, or cyp1a. TCDD induced cyp1a but none of the other genes...
  48. Blanc M, Karrman A, Kukucka P, Scherbak N, Keiter S. Mixture-specific gene expression in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3',4,4',5-pentachlorobiphenyl (PCB126). Sci Total Environ. 2017;590-591:249-257 pubmed publisher
    ..hpf) to 96 hpf to investigate expression changes of genes involved in metabolism of xenobiotics (ahr2, cyp1a), oxidative stress (gpx1a, tp53), lipids metabolism (acaa2, osbpl1a), and epigenetic mechanisms (dnmt1, dnmt3ba)...
  49. Chang C, Chung H, Su H, Tseng H, Tzou W, Hu C. Regulation of zebrafish CYP3A65 transcription by AHR2. Toxicol Appl Pharmacol. 2013;270:174-84 pubmed publisher
    ..It may also reflect the different environments that terrestrial and aquatic organisms encounter. ..
  50. Xiong K, Peterson R, Heideman W. Aryl hydrocarbon receptor-mediated down-regulation of sox9b causes jaw malformation in zebrafish embryos. Mol Pharmacol. 2008;74:1544-53 pubmed publisher
    ..These results suggest that reduced sox9b expression in TCDD-exposed zebrafish embryos contributes to jaw malformation. ..
  51. Chetty S, Praneetha S, Basu S, Sachidanandan C, Murugan A. Sustainable, Rapid Synthesis of Bright-Luminescent CuInS2-ZnS Alloyed Nanocrystals: Multistage Nano-xenotoxicity Assessment and Intravital Fluorescence Bioimaging in Zebrafish-Embryos. Sci Rep. 2016;6:26078 pubmed publisher
  52. Baker B, Yee J, Meyer D, Yang D, Baker T. Histological and Transcriptomic Changes in Male Zebrafish Testes Due to Early Life Exposure to Low Level 2,3,7,8-Tetrachlorodibenzo-p-Dioxin. Zebrafish. 2016;13:413-23 pubmed publisher
    ..These data will inform future investigations to elucidate the mechanism of adult-onset and transgenerational infertility due to TCDD exposure in zebrafish. ..
  53. Christen V, Fent K. Tissue-, sex- and development-specific transcription profiles of eight UDP-glucuronosyltransferase genes in zebrafish (Danio rerio) and their regulation by activator of aryl hydrocarbon receptor. Aquat Toxicol. 2014;150:93-102 pubmed publisher
    ..zebrafish eleuthero-embryos were exposed to 5, 25 and 50?g/L benzo(a)pyrene (BaP), a model ahr2 regulator for cyp1a. Besides transcriptional induction of ahr2 and cyp1a, BaP produced a significant induction of Ugt1a, Ugt5a1, ..
  54. Williams A, Eason J, Chawla B, Bohnsack B. Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway. Invest Ophthalmol Vis Sci. 2017;58:1084-1097 pubmed publisher
    ..These studies provide insight into the role of Cyp1b1 in eye development and further elucidate the pathogenesis of primary infantile-onset glaucoma. ..
  55. Pelayo S, López Roldán R, Gonzalez S, Casado M, Raldua D, Cortina J, et al. A zebrafish scale assay to monitor dioxin-like activity in surface water samples. Anal Bioanal Chem. 2011;401:1861-9 pubmed publisher
    ..It ranks among the very few described protocols able to detect biological effects from natural water samples, without a pre-concentration step, and after only 24 h of exposure. ..
  56. Ma Y, Cao C, Wang Q, Gui W, Zhu G. Effects of azocyclotin on gene transcription and steroid metabolome of hypothalamic-pituitary-gonad axis, and their consequences on reproduction in zebrafish (Danio rerio). Aquat Toxicol. 2016;179:55-64 pubmed publisher
    ..endocrine pathways in the pituitary (lh?), gonad (cyp19a1a, cyp17a1 and 17?-hsd3), and liver (vtg1, vtg2, cyp1a1, comt, ugt1a and gstp1) were correlated with significant reductions in estrogen in both sexes and increased ..
  57. Gerger C, Weber L. Comparison of the acute effects of benzo-a-pyrene on adult zebrafish (Danio rerio) cardiorespiratory function following intraperitoneal injection versus aqueous exposure. Aquat Toxicol. 2015;165:19-30 pubmed publisher
    ..from more severe bradycardia than aqueously exposed fish and had greater levels of increases in cytochrome P4501A (CYP1A) mRNA levels in liver and heart tissue compared to aqueous exposed fish...
  58. Mesquita S, van Drooge B, Oliveira E, Grimalt J, Barata C, Vieira N, et al. Differential embryotoxicity of the organic pollutants in rural and urban air particles. Environ Pollut. 2015;206:535-42 pubmed publisher
    ..The observed effects can be directly related to air pollution-related human disorders, suggesting different potential adverse outcomes for human populations exposed to air pollution from specific sources. ..
  59. Stancová V, Ziková A, Svobodová Z, Kloas W. Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio). Environ Toxicol Pharmacol. 2015;40:343-8 pubmed publisher
    ..Interestingly, the adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study that has focused on transcriptional effects of naproxen on zebrafish. ..
  60. Sanden M, Ornsrud R, Sissener N, Jorgensen S, Gu J, Bakke A, et al. Cross-generational feeding of Bt (Bacillus thuringiensis)-maize to zebrafish (Danio rerio) showed no adverse effects on the parental or offspring generations. Br J Nutr. 2013;110:2222-33 pubmed publisher
    ..In conclusion, it is suggested that Bt-maize is as safe and nutritious as its nBt control when fed to zebrafish for two generations...
  61. Lu X, Yu R, Murphy M, Lau K, Wu R. Hypoxia disrupts gene modulation along the brain-pituitary-gonad (BPG)-liver axis. Ecotoxicol Environ Saf. 2014;102:70-8 pubmed publisher increase in hormone transport and reduced bioavailability in blood, while upregulation of hepatic CYP3A65 and CYP1A in females suggests an increase in estrogen biotransformation and clearance...
  62. Du M, Fang C, Qiu L, Dong S, Zhang X, Yan C. Diastereoisomer-specific effects of hexabromocyclododecanes on hepatic aryl hydrocarbon receptors and cytochrome P450s in zebrafish (Danio rerio). Chemosphere. 2015;132:24-31 pubmed publisher
    ..and γ-HBCD inhibited cyp1a expression but enhanced the expression of cyp1b1 and cyp1c1...
  63. Redelstein R, Zielke H, Spira D, Feiler U, Erdinger L, Zimmer H, et al. Bioaccumulation and molecular effects of sediment-bound metals in zebrafish embryos. Environ Sci Pollut Res Int. 2015;22:16290-304 pubmed publisher
    ..rerio embryos directly exposed to sediments and that the induction of specific genes can be used as biomarkers for the exposure of early life stages of zebrafish to metal-contaminated sediments. ..
  64. Ito Y, Matsuda Y, Suzuki T. Effects of 3,4-dichloroaniline on expression of ahr2 and cyp1a1 in zebrafish adults and embryos. Comp Biochem Physiol C Toxicol Pharmacol. 2010;152:189-94 pubmed publisher
    Arylhydrocarbon receptor (Ahr) and cytochrome P4501a1 (Cyp1a1) are members of the Ahr/Cyp1a1 pathway that oxygenates various toxic chemicals including aryl hydrocarbons...
  65. Sanden M, Jørgensen S, Hemre G, Ørnsrud R, Sissener N. Zebrafish (Danio rerio) as a model for investigating dietary toxic effects of deoxynivalenol contamination in aquaculture feeds. Food Chem Toxicol. 2012;50:4441-8 pubmed publisher
    ..Zebrafish performance was not affected. Liver CYP1A mRNA levels were significantly higher in fish fed 2.0 ppm DON compared to the control group, 0.1, 0.5 and 1...
  66. Jeffries M, Stultz A, Smith A, Stephens D, Rawlings J, Belanger S, et al. The fish embryo toxicity test as a replacement for the larval growth and survival test: A comparison of test sensitivity and identification of alternative endpoints in zebrafish and fathead minnows. Environ Toxicol Chem. 2015;34:1369-81 pubmed publisher
    ..The results not only show the utility of the fathead minnow FET test as a replacement for the LGS test but also provide evidence that inclusion of additional endpoints could improve the predictive power of the FET test. ..
  67. Gerlach C, Das S, Volz D, Bisson W, Kolluri S, Tanguay R. Mono-substituted isopropylated triaryl phosphate, a major component of Firemaster 550, is an AHR agonist that exhibits AHR-independent cardiotoxicity in zebrafish. Aquat Toxicol. 2014;154:71-9 pubmed publisher
    ..that mITP may bind favorably to both AHR2 and AHR1B isoforms, we evaluated AHR involvement in vivo by measuring CYP1A mRNA and protein expression following exposure to mITP in the presence or absence of CH223191 or AHR-specific ..
  68. Stegeman J, Behrendt L, Woodin B, Kubota A, Lemaire B, Pompon D, et al. Functional characterization of zebrafish cytochrome P450 1 family proteins expressed in yeast. Biochim Biophys Acta. 2015;1850:2340-52 pubmed publisher
    Zebrafish express five cytochrome P450 1 genes: CYP1A, CYP1B1, CYP1C1, CYP1C2, inducible by aryl hydrocarbon receptor agonists, and CYP1D1, a constitutively expressed CYP1A-like gene...
  69. Dale K, Rasinger J, Thorstensen K, Penglase S, Ellingsen S. Vitamin E reduces endosulfan-induced toxic effects on morphology and behavior in early development of zebrafish (Danio rerio). Food Chem Toxicol. 2017;101:84-93 pubmed publisher
    ..These results demonstrate that α-TOC protects against phenotypic and behavioral effects caused by ESF but did not rescue ESF-induced aberrations in antioxidant gene expression. ..
  70. Wincent E, Jönsson M, Bottai M, Lundstedt S, Dreij K. Aryl hydrocarbon receptor activation and developmental toxicity in zebrafish in response to soil extracts containing unsubstituted and oxygenated PAHs. Environ Sci Technol. 2015;49:3869-77 pubmed publisher
    ..In addition to Ahr activating potency, the profile of cytochrome P4501 inhibitors may also determine the toxic potency of the extracts. ..
  71. McGee S, Konstantinov A, Stapleton H, Volz D. Aryl phosphate esters within a major PentaBDE replacement product induce cardiotoxicity in developing zebrafish embryos: potential role of the aryl hydrocarbon receptor. Toxicol Sci. 2013;133:144-56 pubmed publisher
    ..Overall, our findings suggest that two major APE components of FM550 induce severe cardiac abnormalities during early vertebrate development. ..
  72. Pang S, Wang H, Li K, Zhu Z, Kang J, Sun Y. Double transgenesis of humanized fat1 and fat2 genes promotes omega-3 polyunsaturated fatty acids synthesis in a zebrafish model. Mar Biotechnol (NY). 2014;16:580-93 pubmed publisher
    ..Our results demonstrate that application of transgenic technology of humanized fat1 and fat2 in farmed fishes can largely improve the n-3 LC-PUFA production. ..
  73. Sánchez Garayzar A, Bahamonde P, Martyniuk C, Betancourt M, Munkittrick K. Hepatic gene expression profiling in zebrafish (Danio rerio) exposed to the fungicide chlorothalonil. Comp Biochem Physiol Part D Genomics Proteomics. 2016;19:102-111 pubmed publisher
    ..This study improves knowledge regarding whole animal exposures to chlorothalonil and identifies molecular signaling cascades that are sensitive to this fungicide in the fish liver. ..
  74. Yin H, Tseng H, Chung H, Ko C, Tzou W, Buhler D, et al. Influence of TCDD on zebrafish CYP1B1 transcription during development. Toxicol Sci. 2008;103:158-68 pubmed publisher
    ..Because knockdown of CYP1B1 did not prevent TCDD-induced pericardial edema and cranial defects, it suggests that CYP1B1 is not involved in the developmental toxicity of dioxin. ..
  75. Bugel S, White L, Cooper K. Inhibition of vitellogenin gene induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin is mediated by aryl hydrocarbon receptor 2 (AHR2) in zebrafish (Danio rerio). Aquat Toxicol. 2013;126:1-8 pubmed publisher
    ..This work provides evidence for AHR2 dependent cross-talk inhibition of vitellogenin genes and offers insight into anti-estrogenic reproductive effects observed in oviparous species exposed to AHR agonist contaminants. ..
  76. DeGroot D, Franks D, Higa T, Tanaka J, Hahn M, Denison M. Naturally occurring marine brominated indoles are aryl hydrocarbon receptor ligands/agonists. Chem Res Toxicol. 2015;28:1176-85 pubmed publisher
    ..Three of the brominated indoles induced CYP1A1 mRNA in human HepG2 cells in vitro and Cyp1a mRNA in zebrafish embryos in vivo...
  77. Zhou Z, Yang J, Chan K. Toxic effects of triclosan on a zebrafish (Danio rerio) liver cell line, ZFL. Aquat Toxicol. 2017;191:175-188 pubmed publisher
    ..Ethoxyresorufin-o-deethylase (EROD) assay suggested that TCS is a weak P4501a (Cyp1a) agonist at 5?M and that it inhibits cytochrome Cyp1a activity induced by benzo(a)pyrene (BaP)...
  78. McIntyre J, Edmunds R, Redig M, Mudrock E, Davis J, Incardona J, et al. Confirmation of Stormwater Bioretention Treatment Effectiveness Using Molecular Indicators of Cardiovascular Toxicity in Developing Fish. Environ Sci Technol. 2016;50:1561-9 pubmed publisher
    ..Molecular markers were more sensitive than visible toxicity indicators, and several cardiac-related genes show promise as novel tools for evaluating the effectiveness of evolving stormwater mitigation strategies. ..
  79. Duan J, Hu H, Zhang Y, Feng L, Shi Y, Miller M, et al. Multi-organ toxicity induced by fine particulate matter PM2.5 in zebrafish (Danio rerio) model. Chemosphere. 2017;180:24-32 pubmed publisher
    ..neural function (gabrd, chrna3, npy8br) were markedly down-regulated; while genes linked to hepatic metabolism (cyp1a, cyp1b1, cyp1c1) were significantly up-regulated by PM2.5. In summary, our data showed that PM2...
  80. Dos Anjos N, Schulze T, Brack W, Val A, Schirmer K, Scholz S. Identification and evaluation of cyp1a transcript expression in fish as molecular biomarker for petroleum contamination in tropical fresh water ecosystems. Aquat Toxicol. 2011;103:46-52 pubmed publisher
    ..Therefore, the transcript expression of various potentially sensitive genes (ahr2(1), cyp1a, hmox1, hsp70, maft, mt, nfe212, gstp1 and nqo1) in fish exposed to water soluble fractions of oil (WSF) was ..
  81. Šrut M, Bourdineaud J, Štambuk A, Klobučar G. Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line. J Appl Toxicol. 2015;35:1381-9 pubmed publisher
  82. Yang J, Zhu J, Chan K. BDE-99, but not BDE-47, is a transient aryl hydrocarbon receptor agonist in zebrafish liver cells. Toxicol Appl Pharmacol. 2016;305:203-215 pubmed publisher
    ..BDE-99 was found to significantly induce cytochrome P450 (CYP1A), uridine diphosphate glucuronosyl transferase 1 family a, b (ugt1ab), 7-ethoxyresorufin-O-deethylase activity and ..
  83. Mesquita S, Dachs J, van Drooge B, Castro Jiménez J, Navarro Martín L, Barata C, et al. Toxicity assessment of atmospheric particulate matter in the Mediterranean and Black Seas open waters. Sci Total Environ. 2016;545-546:163-70 pubmed publisher
    ..test (the ZET assay) showed no major phenotypical adverse effects, up-regulation of mRNA expression of cyp1a, fos and development-related genes (previously described as related to PM toxicity) was observed in exposed ..
  84. Balci T, Prykhozhij S, Teh E, Da as S, McBride E, Liwski R, et al. A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis. Br J Haematol. 2014;167:48-61 pubmed publisher
    ..This study represents the first zebrafish model of a mast cell disease with an aggressive adult phenotype and embryonic markers that could be exploited to screen for novel agents in SM. ..
  85. Xu T, Wang Q, Shi Q, Fang Q, Guo Y, Zhou B. Bioconcentration, metabolism and alterations of thyroid hormones of Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) in Zebrafish. Environ Toxicol Pharmacol. 2015;40:581-6 pubmed publisher
    ..The mRNA expression of genes related to Phase I and II metabolic enzymes, such as cyp1a1, cyp1b1, cyp1c1 and ugt1ab were also significantly upregulated...
  86. Grimes A, Erwin K, Stadt H, Hunter G, Gefroh H, Tsai H, et al. PCB126 exposure disrupts zebrafish ventricular and branchial but not early neural crest development. Toxicol Sci. 2008;106:193-205 pubmed publisher
    ..HLHS is a severe congenital cardiovascular malformation that has previously been linked to industrial releases of dioxins and PCBs. ..
  87. Jenny M, Aluru N, Hahn M. Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos. Toxicol Appl Pharmacol. 2012;264:262-73 pubmed publisher
    ..TCDD caused strong induction of CYP1A at 36hpf (62-fold) and 60hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the ..