Gene Symbol: cyp19a1b
Description: cytochrome P450, family 19, subfamily A, polypeptide 1b
Alias: AroB, CYP19a2, P450aromB, cyp19, cyp19b, zgc:92614, cytochrome P450, family 19, subfamily A, polypeptide 1b, aromatase B, brain aromatase, cytochrome P450 aromatase, cytochrome P450, 19b
Species: zebrafish
Products:     cyp19a1b

Top Publications

  1. Von Hofsten J, Olsson P. Zebrafish sex determination and differentiation: involvement of FTZ-F1 genes. Reprod Biol Endocrinol. 2005;3:63 pubmed
    ..The Cyp19 gene product aromatase converts testosterone into 17 beta-estradiol, and when inhibited leads to male to female ..
  2. Chiang E, Yan Y, Guiguen Y, Postlethwait J, Chung Bc -. Two Cyp19 (P450 aromatase) genes on duplicated zebrafish chromosomes are expressed in ovary or brain. Mol Biol Evol. 2001;18:542-50 pubmed
    b>Cytochrome P450 aromatase (Cyp19) is an enzyme catalyzing the synthesis of estrogens, thereby controlling various physiological functions of estrogens. We isolated two cyp19 cDNAs, termed cyp19a and cyp19b, respectively, from zebrafish...
  3. Tong S, Chung B. Analysis of zebrafish cyp19 promoters. J Steroid Biochem Mol Biol. 2003;86:381-6 pubmed
    ..In zebrafish, two cyp19 genes, cyp19a and cyp19b, expressed in ovary and brain, respectively, were found...
  4. Mouriec K, Gueguen M, Manuel C, Percevault F, Thieulant M, Pakdel F, et al. Androgens upregulate cyp19a1b (aromatase B) gene expression in the brain of zebrafish (Danio rerio) through estrogen receptors. Biol Reprod. 2009;80:889-96 pubmed publisher
    ..Among these features is the high sensitivity of aromatase B (the product of cyp19a1b) to estrogens...
  5. Tong S, Mouriec K, Kuo M, Pellegrini E, Gueguen M, Brion F, et al. A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Genesis. 2009;47:67-73 pubmed publisher the brain, we generated transgenic zebrafish that expresses green fluorescent protein (GFP) driven by the brain aromatase cyp19a1b promoter...
  6. Pellegrini E, Mouriec K, Anglade I, Menuet A, Le Page Y, Gueguen M, et al. Identification of aromatase-positive radial glial cells as progenitor cells in the ventricular layer of the forebrain in zebrafish. J Comp Neurol. 2007;501:150-67 pubmed
    ..Here, we further document that Aromatase B is never detected in cells expressing the markers of postmitotic neurons, Hu and acetylated tubulin...
  7. Pellegrini E, Menuet A, Lethimonier C, Adrio F, Gueguen M, Tascon C, et al. Relationships between aromatase and estrogen receptors in the brain of teleost fish. Gen Comp Endocrinol. 2005;142:60-6 pubmed
    ..are known for exhibiting a high aromatase activity mainly due to the expression of the cyp19b gene, encoding aromatase B (AroB)...
  8. Martyniuk C, Gerrie E, Popesku J, Ekker M, Trudeau V. Microarray analysis in the zebrafish (Danio rerio) liver and telencephalon after exposure to low concentration of 17alpha-ethinylestradiol. Aquat Toxicol. 2007;84:38-49 pubmed
  9. Goto Kazeto R, Kight K, Zohar Y, Place A, Trant J. Localization and expression of aromatase mRNA in adult zebrafish. Gen Comp Endocrinol. 2004;139:72-84 pubmed the control of many behavioral and physiological aspects of reproduction therefore the expression of cytochrome P450 aromatase (CYP19), the enzyme responsible for the conversion of androgens to estrogens, is of vital interest...

More Information


  1. Vosges M, Le Page Y, Chung B, Combarnous Y, Porcher J, Kah O, et al. 17alpha-ethinylestradiol disrupts the ontogeny of the forebrain GnRH system and the expression of brain aromatase during early development of zebrafish. Aquat Toxicol. 2010;99:479-91 pubmed publisher
    ..In parallel, the ER-regulated expression of cytochrome P450 aromatase B (AroB) protein, which is encoded by the cyp19a1b gene, was precisely mapped at the brain and pituitary ..
  2. Mouriec K, Lareyre J, Tong S, Le Page Y, Vaillant C, Pellegrini E, et al. Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development. Dev Dyn. 2009;238:2641-51 pubmed publisher
    ..Early expression of estrogen receptors (esr) and their role in regulating early expression of cyp19a1b encoding brain aromatase were examined in the brain of zebrafish...
  3. Gorelick D, Halpern M. Visualization of estrogen receptor transcriptional activation in zebrafish. Endocrinology. 2011;152:2690-703 pubmed publisher
    ..The transgenic estrogen-responsive zebrafish allow ER signaling to be monitored visually and serve as in vivo sentinels for detection of estrogenic compounds. ..
  4. McCurley A, Callard G. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Mol Biol. 2008;9:102 pubmed publisher
    ..When the accuracy of QPCR analysis was tested using different reference genes to measure suppression of cyp19a1b by an estrogen receptor antagonist and induction of cyp1a by an arylhydrocarbon receptor agonist, the direction ..
  5. Diotel N, Do Rego J, Anglade I, Vaillant C, Pellegrini E, Gueguen M, et al. Activity and expression of steroidogenic enzymes in the brain of adult zebrafish. Eur J Neurosci. 2011;34:45-56 pubmed publisher
    ..By combining aromatase B immunohistochemistry with in situ hybridization, we show that cyp11a1, 3?-hsd and cyp17 messengers are found in ..
  6. Kallivretaki E, Eggen R, Neuhauss S, Kah O, Segner H. The zebrafish, brain-specific, aromatase cyp19a2 is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation. Dev Dyn. 2007;236:3155-66 pubmed
    ..The aim of the current study has been to follow the expression of the brain-specific aromatase cyp19a2 in the brains of sexually differentiating zebrafish...
  7. März M, Chapouton P, Diotel N, Vaillant C, Hesl B, Takamiya M, et al. Heterogeneity in progenitor cell subtypes in the ventricular zone of the zebrafish adult telencephalon. Glia. 2010;58:870-88 pubmed publisher, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of ..
  8. Sassi Messai S, Gibert Y, Bernard L, Nishio S, Ferri Lagneau K, Molina J, et al. The phytoestrogen genistein affects zebrafish development through two different pathways. PLoS ONE. 2009;4:e4935 pubmed publisher
    ..This suggests that the use of standardized endpoints to study the effect of a given compound, even when this compound has well known targets, may carry the risk of overlooking interesting effects of this compound. ..
  9. Le Page Y, Menuet A, Kah O, Pakdel F. Characterization of a cis-acting element involved in cell-specific expression of the zebrafish brain aromatase gene. Mol Reprod Dev. 2008;75:1549-57 pubmed publisher
    The cytochrome P450 Aromatase is the key enzyme catalyzing the conversion of androgens into estrogens. In zebrafish, the brain aromatase is encoded by cyp19b...
  10. Andersen L, Goto Kazeto R, Trant J, Nash J, Korsgaard B, Bjerregaard P. Short-term exposure to low concentrations of the synthetic androgen methyltestosterone affects vitellogenin and steroid levels in adult male zebrafish (Danio rerio). Aquat Toxicol. 2006;76:343-52 pubmed
    ..vitellogenin (VTG) concentration, estradiol (E2), testosterone (T), and 11-ketotestosterone (KT) content, brain aromatase activity and gene expression of CYP19A1 and CYP19A2 in the testis. Exposure to the lowest MT concentration (4...
  11. Kishida M, Callard G. Distinct cytochrome P450 aromatase isoforms in zebrafish (Danio rerio) brain and ovary are differentially programmed and estrogen regulated during early development. Endocrinology. 2001;142:740-50 pubmed neurodevelopment, a PCR cloning strategy was used to isolate complementary DNAs encoding two distinct cytochrome P450 aromatase isoforms in adult zebrafish (Danio rerio) brain and ovary (termed P450aromB and P450aromA, respectively)...
  12. Menuet A, Pellegrini E, Brion F, Gueguen M, Anglade I, Pakdel F, et al. Expression and estrogen-dependent regulation of the zebrafish brain aromatase gene. J Comp Neurol. 2005;485:304-20 pubmed aromatize androgens into estrogens, and this metabolic activity results from the expression of a specific brain aromatase (AroB) generated by the cyp19b gene...
  13. Hinfray N, Palluel O, Turies C, Cousin C, Porcher J, Brion F. Brain and gonadal aromatase as potential targets of endocrine disrupting chemicals in a model species, the zebrafish (Danio rerio). Environ Toxicol. 2006;21:332-7 pubmed
    ..We showed that CYP19b gene was predominantly expressed in the brain whereas in the ovary CYP19a mRNA level was predominant...
  14. Le Page Y, Scholze M, Kah O, Pakdel F. Assessment of xenoestrogens using three distinct estrogen receptors and the zebrafish brain aromatase gene in a highly responsive glial cell system. Environ Health Perspect. 2006;114:752-8 pubmed
    The brain cytochrome P450 aromatase (Aro-B) in zebrafish is expressed in radial glial cells and is strongly stimulated by estrogens (E2); thus, it can be used in vivo as a biomarker of xenoestrogen effects on the central nervous system...
  15. Diotel N, Vaillant C, Gueguen M, Mironov S, Anglade I, Servili A, et al. Cxcr4 and Cxcl12 expression in radial glial cells of the brain of adult zebrafish. J Comp Neurol. 2010;518:4855-76 pubmed publisher
    ..radial glial cell markers, such as brain lipid-binding protein (Blbp) or the estrogen-synthesizing enzyme aromatase B (Cyp19a1b)...
  16. Sawyer S, Gerstner K, Callard G. Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation. Gen Comp Endocrinol. 2006;147:108-17 pubmed
    In teleost fish, the predominant brain form of cytochrome P450 aromatase (P450aromB) is a neural marker of estrogen effect, and an entry point for studying the role of hormonal and environmental estrogens on neurodevelopment and ..
  17. Pradhan A, Asnake S, Kharlyngdoh J, Modig C, Olsson P. In silico and biological analysis of anti-androgen activity of the brominated flame retardants ATE, BATE and DPTE in zebrafish. Chem Biol Interact. 2015;233:35-45 pubmed publisher
    ..Genes involved in steroidogenesis were also down-regulated by these BFRs. In view of this, the impact of these BFRs on humans and wildlife needs further analysis. ..
  18. Tong S, Hsu H, Chung B. Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation. Dev Biol. 2010;344:849-56 pubmed publisher
    ..Our genetic selection scheme matches the prediction that female-dominant genetic factors are required to determine zebrafish sex...
  19. Di Rosa V, López Olmeda J, Burguillo A, Frigato E, Bertolucci C, Piferrer F, et al. Daily Rhythms of the Expression of Key Genes Involved in Steroidogenesis and Gonadal Function in Zebrafish. PLoS ONE. 2016;11:e0157716 pubmed publisher
    ..In the brain, cyp19a1b (brain aromatase) and cyp11b (11beta-hydroxylase) presented daily differences, especially in males, where the expression ..
  20. Callard G, Tchoudakova A, Kishida M, Wood E. Differential tissue distribution, developmental programming, estrogen regulation and promoter characteristics of cyp19 genes in teleost fish. J Steroid Biochem Mol Biol. 2001;79:305-14 pubmed
    ..Multiple cytochrome P450 aromatase (P450arom) cDNAs that derive from separate gene loci (cyp19a and cyp19b) are differentially expressed in ..
  21. Ju B, Chen W, Spitsbergen J, Lu J, Vogel P, Peters J, et al. Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway. Oncogenesis. 2014;3:e96 pubmed publisher
    ..gene signatures characteristic of radial glia and progenitor cells as transcriptions of radial glia genes cyp19a1b, s100?, blbp, gfap and the stem/progenitor genes nestin and sox2 were significantly upregulated...
  22. Hoffmann J, Oris J. Altered gene expression: a mechanism for reproductive toxicity in zebrafish exposed to benzo[a]pyrene. Aquat Toxicol. 2006;78:332-40 pubmed
    ..stimulating hormone (FSH) and luteinizing hormone (LH)), steroidogenic enzymes (CYP11A1, CYP17, CYP19A1, CYP19A2 and 20beta-HSD), estrogen receptor beta (ERbeta) and vitellogenin...
  23. Jin Y, Chen R, Sun L, Wang W, Zhou L, Liu W, et al. Enantioselective induction of estrogen-responsive gene expression by permethrin enantiomers in embryo-larval zebrafish. Chemosphere. 2009;74:1238-44 pubmed publisher
    ..that a 7 d exposure to 250 ng L(-1) PM racemate and its enantiomers was sufficient to stimulate vtg1, esralpha and cyp19b expression, while 1000 ng L(-1) exposure significantly induced gene expression in a pattern similar to that of the ..
  24. Kwon B, Ha N, Jung J, Kim P, Kho Y, Choi K, et al. Effects of Barium Chloride Exposure on Hormones and Genes of the Hypothalamic-Pituitary-Gonad Axis, and Reproduction of Zebrafish (Danio rerio). Bull Environ Contam Toxicol. 2016;96:341-6 pubmed publisher
    ..The results demonstrated that BaCl2 could modulate gene transcriptions and hormone production of the HPG axis in a sex-dependent way, which could cause adverse effects on reproduction and the development of offspring. ..
  25. Cano Nicolau J, Garoche C, Hinfray N, Pellegrini E, Boujrad N, Pakdel F, et al. Several synthetic progestins disrupt the glial cell specific-brain aromatase expression in developing zebra fish. Toxicol Appl Pharmacol. 2016;305:12-21 pubmed publisher
    ..of synthetic ligands of the nuclear progesterone receptor on the glial-specific expression of the zebrafish brain aromatase (cyp19a1b) using zebrafish mechanism-based assays...
  26. Kanungo J, Cuevas E, Guo X, Lopez A, Ramírez Lee M, Trickler W, et al. Nicotine alters the expression of molecular markers of endocrine disruption in zebrafish. Neurosci Lett. 2012;526:133-7 pubmed publisher
    ..the expression of the validated endocrine disruption (ED) biomarkers, vitellogenin (vtg 1 and vtg 2) and cytochrome p450 aromatase (cyp19a1a and cyp19a1b) at the transcriptional level...
  27. Siegenthaler P, Zhao Y, Zhang K, Fent K. Reproductive and transcriptional effects of the antiandrogenic progestin chlormadinone acetate in zebrafish (Danio rerio). Environ Pollut. 2017;223:346-356 pubmed publisher
    ..The observed effects were weaker than those of other very potent progestins, which is probably related to the lack of interaction of CMA with the zebrafish progesterone receptor. ..
  28. Baumann L, Knörr S, Keiter S, Rehberger K, Volz S, Schiller V, et al. Reversibility of endocrine disruption in zebrafish (Danio rerio) after discontinued exposure to the estrogen 17?-ethinylestradiol. Toxicol Appl Pharmacol. 2014;278:230-7 pubmed publisher
    ..EE2 had a strong feminizing and inhibiting effect on the sexual development of zebrafish. Brain aromatase (cyp19b) mRNA expression showed no clear response, but vitellogenin levels were significantly elevated, gonad ..
  29. Blüthgen N, Meili N, Chew G, Odermatt A, Fent K. Accumulation and effects of the UV-filter octocrylene in adult and embryonic zebrafish (Danio rerio). Sci Total Environ. 2014;476-477:207-17 pubmed publisher
    ..Blood levels of 11-ketotestosterone were not altered. The transcriptomics data suggest that OC mainly affects transcription of genes related to developmental processes in the brain and liver as well as metabolic processes in the liver. ..
  30. Yin Y, Tang H, Liu Y, Chen Y, Li G, Liu X, et al. Targeted Disruption of Aromatase Reveals Dual Functions of cyp19a1a During Sex Differentiation in Zebrafish. Endocrinology. 2017;158:3030-3041 pubmed publisher
    Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation in fish, but its precise roles during sex differentiation are still largely unknown...
  31. Ruiter S, Sippel J, Bouwmeester M, Lommelaars T, Beekhof P, Hodemaekers H, et al. Programmed Effects in Neurobehavior and Antioxidative Physiology in Zebrafish Embryonically Exposed to Cadmium: Observations and Hypothesized Adverse Outcome Pathway Framework. Int J Mol Sci. 2016;17: pubmed
    ..In this way, DNA methylation may be affected at a critical developmental stage, causing the observed apical phenotypes. ..
  32. Zhang K, Zhao Y, Fent K. Occurrence and Ecotoxicological Effects of Free, Conjugated, and Halogenated Steroids Including 17?-Hydroxypregnanolone and Pregnanediol in Swiss Wastewater and Surface Water. Environ Sci Technol. 2017;51:6498-6506 pubmed publisher
    ..Although steroid concentrations are low in Swiss rivers, the possibility of additive effects may be of concern. ..
  33. Puy Azurmendi E, Olivares A, Vallejo A, Ortiz Zarragoitia M, Pina B, Zuloaga O, et al. Estrogenic effects of nonylphenol and octylphenol isomers in vitro by recombinant yeast assay (RYA) and in vivo with early life stages of zebrafish. Sci Total Environ. 2014;466-467:1-10 pubmed publisher
    ..Exposure to 33-OP increased transcriptional levels of er?, vtg and cyp19a1b genes...
  34. Kim D, Seok S, Baek M, Lee H, Na Y, Park S, et al. Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish (Danio rerio). Comp Med. 2009;59:416-23 pubmed
    ..To overcome these limitations, we developed a transient expression assay in zebrafish, into which a brain aromatase (cyp19a1b)-based estrogen-responsive reporter gene was introduced...
  35. Hinfray N, Baudiffier D, Leal M, Porcher J, Aït Aïssa S, Le Gac F, et al. Characterization of testicular expression of P450 17?-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole. Gen Comp Endocrinol. 2011;174:309-17 pubmed publisher
    ..These novel data deserve further studies on the effect of azole fungicides on gonadal steroidogenesis. ..
  36. Jørgensen A, Morthorst J, Andersen O, Rasmussen L, Bjerregaard P. Expression profiles for six zebrafish genes during gonadal sex differentiation. Reprod Biol Endocrinol. 2008;6:25 pubmed publisher
    ..have used quantitative real-time PCR to investigate the expression of ar, sox9a, dmrt1, fig alpha, cyp19a1a and cyp19a1b during the expected sex determination and gonadal sex differentiation period...
  37. Pérez M, Pellegrini E, Cano Nicolau J, Gueguen M, Menouer Le Guillou D, Mérot Y, et al. Relationships between radial glial progenitors and 5-HT neurons in the paraventricular organ of adult zebrafish - potential effects of serotonin on adult neurogenesis. Eur J Neurosci. 2013;38:3292-301 pubmed publisher
    ..between RGCs and 5-HT CSF-contacting neurons, we performed 5-HT immunohistochemistry in transgenic tg(cyp19a1b-GFP) zebrafish in which RGCs are labelled with GFP under the control of the cyp19a1b promoter...
  38. Lassiter C, Linney E. Embryonic expression and steroid regulation of brain aromatase cyp19a1b in zebrafish (Danio rerio). Zebrafish. 2007;4:49-57 pubmed
    Estradiol is produced from testosterone by the aromatase gene, cyp19. In the zebrafish Danio rerio, brain aromatase, cyp19a1b, is highly expressed during development...
  39. Suzawa M, Ingraham H. The herbicide atrazine activates endocrine gene networks via non-steroidal NR5A nuclear receptors in fish and mammalian cells. PLoS ONE. 2008;3:e2117 pubmed publisher
  40. Xu Q, Wu D, Dang Y, Yu L, Liu C, Wang J. Reproduction impairment and endocrine disruption in adult zebrafish (Danio rerio) after waterborne exposure to TBOEP. Aquat Toxicol. 2017;182:163-171 pubmed publisher
  41. Nasri A, Valverde A, Roche D, Desrumaux C, Clair P, Beyrem H, et al. Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations. Int J Mol Sci. 2016;17: pubmed publisher
    ..On the other hand, canonical targets of endocrine signaling are not affected, suggesting that the neurotoxic effect of A6 may be due to the binding of this compound to a recently identified, neuron-specific estrogen receptor. ..
  42. Hua J, Han J, Guo Y, Zhou B. The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations. Aquat Toxicol. 2015;166:1-9 pubmed publisher
    ..Down-regulation of the mRNA expression of aromatase (e.g., cyp19a1a, cyp19a1b), the forkhead transcription factor gene L2 (foxl2) and the Fushi tarazu factor-1d (nr5a1b) were observed...
  43. Wang J, Cao X, Sun J, Huang Y, Tang X. Disruption of endocrine function in H295R cell in vitro and in zebrafish in vivo by naphthenic acids. J Hazard Mater. 2015;299:1-9 pubmed publisher
    ..The abundances of transcripts of biomarker gene CYP19b, ERα, and VTG were significantly up-regulated in zebrafish larvae exposed to OS-NAs and C-NAs, which indicated ..
  44. Diotel N, Mérot Y, Coumailleau P, Gueguen M, Sérandour A, Salbert G, et al. 5-hydroxymethylcytosine marks postmitotic neural cells in the adult and developing vertebrate central nervous system. J Comp Neurol. 2017;525:478-497 pubmed publisher
    ..J. Comp. Neurol. 525:478-497, 2017. © 2016 Wiley Periodicals, Inc. ..
  45. Ortiz Villanueva E, Navarro Martín L, Jaumot J, Benavente F, Sanz Nebot V, Pina B, et al. Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomic and transcriptomic approach. Environ Pollut. 2017;231:22-36 pubmed publisher
    ..These results illustrate the utility of -omic integrative analyses for characterizing the physiological effects of toxicants beyond the mere indication of the affected pathways. ..
  46. Filby A, Paull G, Bartlett E, Van Look K, Tyler C. Physiological and health consequences of social status in zebrafish (Danio rerio). Physiol Behav. 2010;101:576-87 pubmed publisher
    ..The wide-ranging physiological differences seen between dominant and subordinate zebrafish as a consequence of their social status suggest negative health impacts for subordinates after prolonged durations in those hierarchies. ..
  47. Driessen M, Kienhuis A, Vitins A, Pennings J, Pronk T, van den Brandhof E, et al. Gene expression markers in the zebrafish embryo reflect a hepatotoxic response in animal models and humans. Toxicol Lett. 2014;230:48-56 pubmed publisher
    ..In conclusion, the ZFE model allows for identification of hepatotoxicants, without discrimination into specific phenotypes. ..
  48. Huang W, Yang P, Lv Z, Wu C, Gui J, Lou B. Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker. Gene. 2017;627:271-277 pubmed publisher
    ..These results indicate that the Mmcyp19a1a gene plays an important role during vitellogenesis and oocyte maturation. The constructor of Mmcyp19a1a:EGFP may provide a useful tool for genetic analysis of gonad development in teleost. ..
  49. Baudiffier D, Hinfray N, Ravaud C, Creusot N, Chadili E, Porcher J, et al. Effect of in vivo chronic exposure to clotrimazole on zebrafish testis function. Environ Sci Pollut Res Int. 2013;20:2747-60 pubmed publisher
    ..Our study demonstrated that clotrimazole is able to affect testicular physiology and raised further concern about the impact of clotrimazole on reproduction. ..
  50. Ji K, Hong S, Kho Y, Choi K. Effects of bisphenol s exposure on endocrine functions and reproduction of zebrafish. Environ Sci Technol. 2013;47:8793-800 pubmed publisher
    ..Our observations showed that exposure to low level BPS could affect the feedback regulatory circuits of HPG axis and impair the development of offspring. ..
  51. Yu R, Chu D, Tan T, Li V, Chan A, Giesy J, et al. Leptin-mediated modulation of steroidogenic gene expression in hypoxic zebrafish embryos: implications for the disruption of sex steroids. Environ Sci Technol. 2012;46:9112-9 pubmed publisher
    ..Overall, this study indicates that hypoxia, possibly via HIF-1-induced leptin expression, modulates sex steroid synthesis by acting directly on steroidogenic gene expression. ..
  52. Ganz J, Kaslin J, Hochmann S, Freudenreich D, Brand M. Heterogeneity and Fgf dependence of adult neural progenitors in the zebrafish telencephalon. Glia. 2010;58:1345-63 pubmed publisher
  53. Truong L, Bugel S, Chlebowski A, Usenko C, Simonich M, Simonich S, et al. Optimizing multi-dimensional high throughput screening using zebrafish. Reprod Toxicol. 2016;65:139-147 pubmed publisher
    ..Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories. ..
  54. Zhang X, Gao L, Yang K, Tian H, Wang W, Ru S. Monocrotophos pesticide modulates the expression of sexual differentiation genes and causes phenotypic feminization in zebrafish (Danio rerio). Comp Biochem Physiol C Toxicol Pharmacol. 2013;157:33-40 pubmed publisher
    ..In addition, MCP treatment increased the transcription of brain aromatase (cyp19a1b), resulting in an indirect impact on sexual differentiation...
  55. Kinch C, Ibhazehiebo K, Jeong J, Habibi H, Kurrasch D. Low-dose exposure to bisphenol A and replacement bisphenol S induces precocious hypothalamic neurogenesis in embryonic zebrafish. Proc Natl Acad Sci U S A. 2015;112:1475-80 pubmed publisher
    ..Furthermore, our results show that BPA-free products are not necessarily safer and support the removal of all bisphenols from consumer merchandise. ..
  56. Neale P, Munz N, AÑ—t AÑ—ssa S, Altenburger R, Brion F, Busch W, et al. Integrating chemical analysis and bioanalysis to evaluate the contribution of wastewater effluent on the micropollutant burden in small streams. Sci Total Environ. 2017;576:785-795 pubmed publisher
  57. Yu L, Liu C, Chen Q, Zhou B. Endocrine disruption and reproduction impairment in zebrafish after long-term exposure to DE-71. Environ Toxicol Chem. 2014;33:1354-62 pubmed publisher
    ..Exposure of female zebrafish to DE-71 resulted in lower estradiol production and downregulation of cytochrome P450 aromatase mRNA...
  58. Kang H, Moon H, Choi K. Toxicological responses following short-term exposure through gavage feeding or water-borne exposure to Dechlorane Plus in zebrafish (Danio rerio). Chemosphere. 2016;146:226-32 pubmed publisher
    ..Our observations indicate for the first time that DP disrupts thyroid hormone balance of zebrafish by altering regulatory pathways in the brain. Handling editor: David Volz. ..
  59. Ji K, Liu X, Lee S, Kang S, Kho Y, Giesy J, et al. Effects of non-steroidal anti-inflammatory drugs on hormones and genes of the hypothalamic-pituitary-gonad axis, and reproduction of zebrafish. J Hazard Mater. 2013;254-255:242-51 pubmed publisher
    ..The results demonstrated that ibuprofen could modulate hormone production and related gene transcription of the HPG axis in a sex-dependent way, which could cause adverse effects on reproduction and the development of offspring. ..
  60. Kim D, Seok S, Baek M, Lee H, Na Y, Park S, et al. Benomyl induction of brain aromatase and toxic effects in the zebrafish embryo. J Appl Toxicol. 2009;29:289-94 pubmed publisher
    ..The brain aromatase gene is up-regulated by estrogens and estrogen mimics and considered a target gene to screen estrogen mimics...
  61. Bouwmeester M, Ruiter S, Lommelaars T, Sippel J, Hodemaekers H, van den Brandhof E, et al. Zebrafish embryos as a screen for DNA methylation modifications after compound exposure. Toxicol Appl Pharmacol. 2016;291:84-96 pubmed publisher
    ..Site-specific methylation was examined in the promoter regions of three selected genes vasa, vtg1 and cyp19a2, of which vasa (ddx4) was the most responsive...
  62. Caulier M, Brion F, Chadili E, Turies C, Piccini B, Porcher J, et al. Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish (Danio rerio). Comp Biochem Physiol A Mol Integr Physiol. 2015;188:96-106 pubmed publisher
    ..pattern of steroidogenic enzymes involved in estrogen and androgen production (Cyp17-I, Cyp11c1, Cyp19a1a and Cyp19a1b) and one of their regulators (Foxl2a) in zebrafish gonads...
  63. Schiller V, Wichmann A, Kriehuber R, Schäfers C, Fischer R, Fenske M. Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption. Reprod Toxicol. 2013;42:210-23 pubmed publisher
    ..This demonstrates that different mechanism of ED can be assessed already in fish embryos. ..
  64. Zucchi S, Blüthgen N, Ieronimo A, Fent K. The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males. Toxicol Appl Pharmacol. 2011;250:137-46 pubmed publisher
    ..In eleuthero-embryos transcripts of vtg1, vtg3, esr1, esr2b, hsd17ß3, cyp19b cyp19a, hhex and pax8 were induced at 3000 ?g/L BP-4, which points to a low estrogenic activity and interference ..
  65. Li X, Guo J, Li X, Zhou H, Zhang S, Liu X, et al. Behavioural effect of low-dose BPA on male zebrafish: Tuning of male mating competition and female mating preference during courtship process. Chemosphere. 2017;169:40-52 pubmed publisher
    ..In sum, our results shed light on the potential behavioural and physiological effect of low-dose BPA exposure on courtship behaviours of zebrafish, which could exert profound consequences on natural zebrafish populations. ..
  66. Sun D, Zhang Y, Wang C, Hua X, Zhang X, Yan J. Sox9-related signaling controls zebrafish juvenile ovary-testis transformation. Cell Death Dis. 2013;4:e930 pubmed publisher
  67. Diotel N, Servili A, Gueguen M, Mironov S, Pellegrini E, Vaillant C, et al. Nuclear progesterone receptors are up-regulated by estrogens in neurons and radial glial progenitors in the brain of zebrafish. PLoS ONE. 2011;6:e28375 pubmed publisher
    ..Given the crucial roles of radial glial cells in adult neurogenesis, the potential effects of progestagens on their activity and the fate of daughter cells require thorough investigation. ..
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    ..Significant up-regulation of esr1 and cyp19b were observed in the gonads, as well as vtg1 in the livers for both female and male zebrafish...
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    ..As such, our results have significant consequences for human and environmental risk assessment. ..
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    ..comparatively evaluated for all compounds for effects on biomarker transcripts indicative of AHR (cyp1a) and ER (cyp19a1b, esr1, lhb, vtg) pathway bioactivity...
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    ..In the brain of adult males esr1, ar and cyp19b are down-regulated at 84 ?g/L BP-3...
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    ..e., 3beta-HSD, CYP11A, CYP19A, and CYP19B) as well as an increase in the testosterone/estradiol ratio...
  74. Cao F, Zhu L, Li H, Yu S, Wang C, Qiu L. Reproductive toxicity of azoxystrobin to adult zebrafish (Danio rerio). Environ Pollut. 2016;219:1109-1121 pubmed publisher
    ..fshr and lhr), steroid hormone receptors (era, er2b and ar), steroidogenic enzymes (cyp11a, cyp11b, cyp17, cyp19a, cyp19b, hsd3b and hsd17b) in the hypothalamic-pituitary-gonad (HPG) axis and vitellogenin (vtg1 and vtg2) in the livers ..
  75. Lima D, Castro L, Coelho I, Lacerda R, Gesto M, Soares J, et al. Effects of Tributyltin and Other Retinoid Receptor Agonists in Reproductive-Related Endpoints in the Zebrafish (Danio rerio). J Toxicol Environ Health A. 2015;78:747-60 pubmed publisher
    ..Significant downregulation was observed in brain mRNA levels of aromatase b (CYP19a1b) in females and peroxisome proliferator activated receptor gamma (PPARg) in both males and females, ..
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    ..In conclusion, this study first investigated the sex-dependent effects of microcystins on fish reproduction and revealed some important molecular biomarkers related to gametogenesis in zebrafish suffered from MC-LR. ..
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    ..those encoding hormone receptors (pgr, esr1), a steroidogenic enzyme (hsd17b3), and estrogenic markers (vtg1, cyp19b), in particular at 144 hpf. In general, DRS showed stronger transcriptional changes than P4...
  78. Yang Q, Yang X, Liu J, Ren W, Chen Y, Shen S. Effects of BPF on steroid hormone homeostasis and gene expression in the hypothalamic-pituitary-gonadal axis of zebrafish. Environ Sci Pollut Res Int. 2017;24:21311-21322 pubmed publisher
    ..These alterations suggest that BPF leads to adverse effects on the endocrine system of teleost fish, and that these effects were more prominent in males than in females. ..
  79. Zucchi S, Oggier D, Fent K. Global gene expression profile induced by the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) in zebrafish (Danio rerio). Environ Pollut. 2011;159:3086-96 pubmed publisher
    ..The analysis showed tissue-specific gene profiles and revealed that EHMC slightly affects the transcription of genes involved in hormonal pathways including vtg1, esr1, esr2b, ar, cyp19b and hsd17?3.
  80. Baumann L, Knörr S, Keiter S, Nagel T, Rehberger K, Volz S, et al. Persistence of endocrine disruption in zebrafish (Danio rerio) after discontinued exposure to the androgen 17?-trenbolone. Environ Toxicol Chem. 2014;33:2488-96 pubmed publisher
    ..Changes of brain aromatase (cyp19b) mRNA expression showed no consistent trend with respect to either exposure duration or concentration...