VP40

Summary

Gene Symbol: VP40
Description: coalesce nucleocapsids and cell membranes in virion assembly (budding)
Alias: coalesce nucleocapsids and cell membranes in virion assembly (budding), matrix protein
Species: Zaire Ebola virus
Products:     VP40

Top Publications

  1. Volchkov V, Volchkova V, Chepurnov A, Blinov V, Dolnik O, Netesov S, et al. Characterization of the L gene and 5' trailer region of Ebola virus. J Gen Virol. 1999;80 ( Pt 2):355-62 pubmed publisher
    ..The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus...
  2. Panchal R, Ruthel G, Kenny T, Kallstrom G, Lane D, Badie S, et al. In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding. Proc Natl Acad Sci U S A. 2003;100:15936-41 pubmed
    The matrix protein VP40 plays a critical role in Ebola virus assembly and budding, a process that utilizes specialized membrane domains known as lipid rafts...
  3. Hartlieb B, Weissenhorn W. Filovirus assembly and budding. Virology. 2006;344:64-70 pubmed publisher
    ..They have a characteristic filamentous shape, which is largely determined by the matrix protein VP40...
  4. Subbotina E, Kachko A, Chepurnov A. [The properties of Ebola virus proteins]. Vopr Virusol. 2006;51:4-10 pubmed
    ..It also presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral ..
  5. Harty R, Brown M, Wang G, Huibregtse J, Hayes F. A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: implications for filovirus budding. Proc Natl Acad Sci U S A. 2000;97:13871-6 pubmed
    VP40, the putative matrix protein of both Ebola and Marburg viruses, possesses a conserved proline-rich motif (PY motif) at its N terminus...
  6. Noda T, Sagara H, Suzuki E, Takada A, Kida H, Kawaoka Y. Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP. J Virol. 2002;76:4855-65 pubmed
    Using biochemical assays, it has been demonstrated that expression of Ebola virus VP40 alone in mammalian cells induced production of particles with a density similar to that of virions...
  7. Timmins J, Schoehn G, Ricard Blum S, Scianimanico S, Vernet T, Ruigrok R, et al. Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4. J Mol Biol. 2003;326:493-502 pubmed
    The Ebola virus matrix protein VP40 is a major viral structural protein and plays a central role in virus assembly and budding at the plasma membrane of infected cells...
  8. Licata J, Simpson Holley M, Wright N, Han Z, Paragas J, Harty R. Overlapping motifs (PTAP and PPEY) within the Ebola virus VP40 protein function independently as late budding domains: involvement of host proteins TSG101 and VPS-4. J Virol. 2003;77:1812-9 pubmed
    The VP40 protein of Ebola virus can bud from mammalian cells in the form of lipid-bound, virus-like particles (VLPs), and late budding domains (L-domains) are conserved motifs (PTAP, PPxY, or YxxL; where "x" is any amino acid) ..
  9. Kallstrom G, Warfield K, Swenson D, Mort S, Panchal R, Ruthel G, et al. Analysis of Ebola virus and VLP release using an immunocapture assay. J Virol Methods. 2005;127:1-9 pubmed
    ..detects both Ebola virus and EBOV-like particles (VLPs) directly from cell-culture supernatants with the VP40 matrix protein serving as antigen...

More Information

Publications55

  1. Ruthel G, Demmin G, Kallstrom G, Javid M, Badie S, Will A, et al. Association of ebola virus matrix protein VP40 with microtubules. J Virol. 2005;79:4709-19 pubmed
    ..Here, we report that Ebola virus associates with microtubules via the matrix protein VP40...
  2. Licata J, Johnson R, Han Z, Harty R. Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles. J Virol. 2004;78:7344-51 pubmed
    The VP40 matrix protein of Ebola virus buds from cells in the form of virus-like particles (VLPs) and plays a central role in virus assembly and budding...
  3. Jasenosky L, Neumann G, Lukashevich I, Kawaoka Y. Ebola virus VP40-induced particle formation and association with the lipid bilayer. J Virol. 2001;75:5205-14 pubmed
    Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle...
  4. Elliott L, Kiley M, McCormick J. Descriptive analysis of Ebola virus proteins. Virology. 1985;147:169-76 pubmed
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  5. Teimoori S, Seesuay W, Jittavisutthikul S, Chaisri U, Sookrung N, Densumite J, et al. Human transbodies to VP40 inhibit cellular egress of Ebola virus-like particles. Biochem Biophys Res Commun. 2016;479:245-252 pubmed publisher
    A direct acting anti-Ebola agent is needed. VP40, a conserved protein across Ebolavirus (EBOV) species has several pivotal roles in the virus life cycle...
  6. Gc J, Pokhrel R, Bhattarai N, Johnson K, Gerstman B, Stahelin R, et al. Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments. Biochem Biophys Res Commun. 2017;493:176-181 pubmed publisher
    ..The Ebola virus matrix protein VP40 exists in various conformational and oligomeric forms and is a potential pharmacological target for ..
  7. Warfield K, Bosio C, Welcher B, Deal E, Mohamadzadeh M, Schmaljohn A, et al. Ebola virus-like particles protect from lethal Ebola virus infection. Proc Natl Acad Sci U S A. 2003;100:15889-94 pubmed
    ..Coexpression of the Ebola virus glycoprotein (GP) and matrix protein (VP40) in mammalian cells results in spontaneous production and release of virus-like particles (VLPs) that ..
  8. Neumann G, Ebihara H, Takada A, Noda T, Kobasa D, Jasenosky L, et al. Ebola virus VP40 late domains are not essential for viral replication in cell culture. J Virol. 2005;79:10300-7 pubmed
    Ebola virus particle formation and budding are mediated by the VP40 protein, which possesses overlapping PTAP and PPXY late domain motifs (7-PTAPPXY-13)...
  9. Warfield K, Swenson D, Demmin G, Bavari S. Filovirus-like particles as vaccines and discovery tools. Expert Rev Vaccines. 2005;4:429-40 pubmed publisher
    ..Expression of the filovirus matrix protein, VP40, is sufficient to drive spontaneous production and release of virus-like particles (VLPs) that resemble ..
  10. Urata S, Noda T, Kawaoka Y, Morikawa S, Yokosawa H, Yasuda J. Interaction of Tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and Tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP. J Virol. 2007;81:4895-9 pubmed publisher
    Marburg virus (MARV) VP40 is a matrix protein that can be released from mammalian cells in the form of virus-like particles (VLPs) and contains the PPPY sequence, which is an L-domain motif...
  11. Theriault S, Groseth A, Neumann G, Kawaoka Y, Feldmann H. Rescue of Ebola virus from cDNA using heterologous support proteins. Virus Res. 2004;106:43-50 pubmed publisher
    ..In contrast to previous data, viral proteins were able to target heterologous filovirus RNA. Together these results indicated that protein-protein interactions are more critical than protein-RNA interactions...
  12. Bukreyev A, Volchkov V, Blinov V, Netesov S. The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses. FEBS Lett. 1993;322:41-6 pubmed
    ..These fragments were found to encode the VP35 and VP40 proteins. The canonic sequences were revealed before and after each open reading frame...
  13. Timmins J, Schoehn G, Kohlhaas C, Klenk H, Ruigrok R, Weissenhorn W. Oligomerization and polymerization of the filovirus matrix protein VP40. Virology. 2003;312:359-68 pubmed
    The matrix protein VP40 from Ebola virus plays an important role in the assembly process of virus particles by interacting with cellular factors, cellular membranes, and the ribonuclearprotein particle complex...
  14. Volchkov V, Feldmann H, Volchkova V, Klenk H. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998;95:5762-7 pubmed
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  15. Irie T, Licata J, McGettigan J, Schnell M, Harty R. Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A. J Virol. 2004;78:2657-65 pubmed
    ..Ebola virus VP40 has overlapping PTAP and PPEY motifs, which can functionally interact with TSG101 and Nedd4, respectively...
  16. Soni S, Stahelin R. The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes. J Biol Chem. 2014;289:33590-7 pubmed publisher
    ..The Ebola virus negative sense RNA genome encodes seven proteins including viral matrix protein 40 (VP40), which is the most abundant protein found in the virions...
  17. Groseth A, Str her U, Theriault S, Feldmann H. Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States. Virus Res. 2002;87:155-63 pubmed
    ..The sequence determination provides the basis for the development of a reverse genetics system for Ebola virus Reston, which is needed to study differences in pathogenicity among filoviruses...
  18. Johnson R, McCarthy S, Godlewski P, Harty R. Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles. J Virol. 2006;80:5135-44 pubmed publisher
    ..The nucleocapsid then interacts with the viral matrix protein, leading to specific packaging of the nucleocapsid into the virion...
  19. Han Z, Harty R. Packaging of actin into Ebola virus VLPs. Virol J. 2005;2:92 pubmed publisher
    ..Our results indicated that actin and Ebola virus VP40 strongly co-localized in transfected cells as determined by confocal microscopy...
  20. Hoenen T, Volchkov V, Kolesnikova L, Mittler E, Timmins J, Ottmann M, et al. VP40 octamers are essential for Ebola virus replication. J Virol. 2005;79:1898-905 pubmed publisher
    b>Matrix protein VP40 of Ebola virus is essential for virus assembly and budding...
  21. Johnson R, Bell P, Harty R. Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology. Virol J. 2006;3:31 pubmed publisher
    Recently we described a role for Ebola virus proteins, NP, GP, and VP35 in enhancement of VP40 VLP budding...
  22. McCarthy S, Johnson R, Zhang Y, Sunyer J, Harty R. Role for amino acids 212KLR214 of Ebola virus VP40 in assembly and budding. J Virol. 2007;81:11452-60 pubmed publisher
    Ebola virus VP40 is able to produce virus-like particles (VLPs) in the absence of other viral proteins...
  23. Jasenosky L, Kawaoka Y. Filovirus budding. Virus Res. 2004;106:181-8 pubmed
    ..will discuss the current state of filovirus budding research, with especial emphasis placed on the viral matrix protein VP40 and its relationship with the cellular vesicular sorting pathway...
  24. Yamayoshi S, Kawaoka Y. Mapping of a region of Ebola virus VP40 that is important in the production of virus-like particles. J Infect Dis. 2007;196 Suppl 2:S291-5 pubmed publisher
    Ebola virus VP40 contains 2 overlapping late domains (7-PTAP-10 and 10-PPEY-13) that are essential for its interaction with Tsg101 and Nedd4 in the promotion of viral egress...
  25. Bornholdt Z, Noda T, Abelson D, Halfmann P, Wood M, Kawaoka Y, et al. Structural rearrangement of ebola virus VP40 begets multiple functions in the virus life cycle. Cell. 2013;154:763-74 pubmed publisher
    ..Here, we illustrate through multiple crystal structures, biochemistry, and cellular microscopy that VP40 rearranges into different structures, each with a distinct function required for the ebolavirus life cycle...
  26. Volchkov V, Becker S, Volchkova V, Ternovoj V, Kotov A, Netesov S, et al. GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases. Virology. 1995;214:421-30 pubmed
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  27. Watanabe S, Noda T, Kawaoka Y. Functional mapping of the nucleoprotein of Ebola virus. J Virol. 2006;80:3743-51 pubmed
    ..Sun, and G. J. Nabel, Mol. Cell 10:307-316, 2002) previously demonstrated that NP, together with the minor matrix protein VP24 and polymerase cofactor VP35, is necessary and sufficient for the formation of nucleocapsid-like ..
  28. Dessen A, Volchkov V, Dolnik O, Klenk H, Weissenhorn W. Crystal structure of the matrix protein VP40 from Ebola virus. EMBO J. 2000;19:4228-36 pubmed
    Ebola virus maturation occurs at the plasma membrane of infected cells and involves the clustering of the viral matrix protein VP40 at the assembly site as well as its interaction with the lipid bilayer...
  29. Lucht A, Grunow R, M ller P, Feldmann H, Becker S. Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus. J Virol Methods. 2003;111:21-8 pubmed
    ..stable hybridoma cell lines were obtained producing specific mAbs directed against the viral structural protein VP40. These mAbs were characterized by enzyme-linked immunosorbent, immunoblot and immunofluorescence assays...
  30. Noda T, Watanabe S, Sagara H, Kawaoka Y. Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus. J Virol. 2007;81:3554-62 pubmed
    ..We recently found that NP binding with the matrix protein VP40 is important for nucleocapsid incorporation into virions (T. Noda, H. Ebihara, Y. Muramoto, K. Fujii, A...
  31. Gc J, Johnson K, Husby M, Frick C, Gerstman B, Stahelin R, et al. Interdomain salt-bridges in the Ebola virus protein VP40 and their role in domain association and plasma membrane localization. Protein Sci. 2016;25:1648-58 pubmed publisher
    The Ebola virus protein VP40 is a transformer protein that possesses an extraordinary ability to accomplish multiple functions by transforming into various oligomeric conformations...
  32. McCarthy S, Licata J, Harty R. A luciferase-based budding assay for Ebola virus. J Virol Methods. 2006;137:115-9 pubmed
    The VP40 matrix protein of Ebola virus (EBOV) is capable of budding from mammalian cells as a virus-like particle (VLP) and is the major protein involved in virus egress...
  33. Shahhosseini S, Das D, Qiu X, Feldmann H, Jones S, Suresh M. Production and characterization of monoclonal antibodies against different epitopes of Ebola virus antigens. J Virol Methods. 2007;143:29-37 pubmed publisher
    ..Seventeen MAbs of different epitope specificities against EBOV antigens [virion protein (VP40), secreted glycoprotein (sGP), and GP(1) subfragment D] were developed...
  34. Lu J, Qu Y, Liu Y, Jambusaria R, Han Z, Ruthel G, et al. Host IQGAP1 and Ebola virus VP40 interactions facilitate virus-like particle egress. J Virol. 2013;87:7777-80 pubmed publisher
    We have identified host IQGAP1 as an interacting partner for Ebola virus (EBOV) VP40, and its expression is required for EBOV VP40 virus-like particle (VLP) budding...
  35. Adu Gyamfi E, Soni S, Xue Y, Digman M, Gratton E, Stahelin R. The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress. J Biol Chem. 2013;288:5779-89 pubmed publisher
    ..genome encodes seven genes, the most abundantly expressed of which is viral protein 40 (VP40), the major Ebola matrix protein that regulates assembly and egress of the virus...
  36. Yamayoshi S, Noda T, Ebihara H, Goto H, Morikawa Y, Lukashevich I, et al. Ebola virus matrix protein VP40 uses the COPII transport system for its intracellular transport. Cell Host Microbe. 2008;3:168-77 pubmed publisher
    The Ebola virus matrix protein VP40 plays an important role in virion formation and viral egress from cells...
  37. Ye L, Lin J, Sun Y, Bennouna S, Lo M, Wu Q, et al. Ebola virus-like particles produced in insect cells exhibit dendritic cell stimulating activity and induce neutralizing antibodies. Virology. 2006;351:260-70 pubmed publisher
    Recombinant baculoviruses (rBV) expressing Ebola virus VP40 (rBV-VP40) or GP (rBV-GP) proteins were generated...
  38. Scianimanico S, Schoehn G, Timmins J, Ruigrok R, Klenk H, Weissenhorn W. Membrane association induces a conformational change in the Ebola virus matrix protein. EMBO J. 2000;19:6732-41 pubmed publisher
    The matrix protein VP40 from Ebola virus is targeted to the plasma membrane, where it is thought to induce assembly and budding of virions through its association with the lipid bilayer...
  39. Tikunova N, Batanova T, Chepurnov A. [Human recombinant antibodies to Ebola virus: preparation and characteristics]. Vopr Virusol. 2005;50:25-9 pubmed
    ..showed that 6 antibodies identified nucleoprotein (NP), one antibody did VP24 and another antibody did VP40. One of the selected antibodies reacted with two EP proteins: VP24 and VP40...
  40. Gomis R th F, Dessen A, Timmins J, Bracher A, Kolesnikowa L, Becker S, et al. The matrix protein VP40 from Ebola virus octamerizes into pore-like structures with specific RNA binding properties. Structure. 2003;11:423-33 pubmed
    The Ebola virus membrane-associated matrix protein VP40 is thought to be crucial for assembly and budding of virus particles...
  41. Martin Serrano J, Zang T, Bieniasz P. HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress. Nat Med. 2001;7:1313-9 pubmed publisher
    ..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
  42. Takada A, Kawaoka Y. The pathogenesis of Ebola hemorrhagic fever. Trends Microbiol. 2001;9:506-11 pubmed
  43. Volchkov V, Volchkova V, Slenczka W, Klenk H, Feldmann H. Release of viral glycoproteins during Ebola virus infection. Virology. 1998;245:110-9 pubmed publisher
    ..The abundant shedding of soluble GP1 may play an important role in the immunopathology of Ebola hemorrhagic fever in experimentally and naturally infected hosts...
  44. Anderson G, Liu J, Zabetakis D, Legler P, Goldman E. Label free checkerboard assay to determine overlapping epitopes of Ebola virus VP-40 antibodies using surface plasmon resonance. J Immunol Methods. 2017;442:42-48 pubmed publisher
    ..All the antibodies targeted the Ebola virus viral protein 40 (VP40)...
  45. Fabozzi G, Nabel C, Dolan M, Sullivan N. Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J Virol. 2011;85:2512-23 pubmed publisher
    ..In addition to viral protein 35 (VP35), we found that VP30 and VP40 independently act as SRSs. Here, we present the molecular mechanisms of VP30 and VP35...
  46. Murray J, Mavrakis M, McDonald N, Yilla M, Sheng J, Bellini W, et al. Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus. J Virol. 2005;79:11742-51 pubmed publisher
    ..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...