VP35

Summary

Gene Symbol: VP35
Description: polymerase complex protein
Species: Zaire Ebola virus
Products:     VP35

Top Publications

  1. Enterlein S, Warfield K, Swenson D, Stein D, Smith J, Gamble C, et al. VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice. Antimicrob Agents Chemother. 2006;50:984-93 pubmed
    ..A 22-mer P-PMO designed to base pair with the translation start site region of EBOV VP35 positive-sense RNA generated sequence-specific and time- and dose-dependent inhibition of EBOV amplification in ..
  2. Cardenas W, Loo Y, Gale M, Hartman A, Kimberlin C, Martinez Sobrido L, et al. Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. J Virol. 2006;80:5168-78 pubmed
    The Ebola virus (EBOV) VP35 protein blocks the virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF-3), a transcription factor critical for the induction of alpha/beta interferon (IFN-alpha/beta) expression...
  3. Basler C, Wang X, Muhlberger E, Volchkov V, Paragas J, Klenk H, et al. The Ebola virus VP35 protein functions as a type I IFN antagonist. Proc Natl Acad Sci U S A. 2000;97:12289-94 pubmed
    ..Using this assay, we have identified an Ebola virus-encoded inhibitor of the type I IFN response, the Ebola virus VP35 protein...
  4. Hartman A, Dover J, Towner J, Nichol S. Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication. J Virol. 2006;80:6430-40 pubmed
    The VP35 protein of Zaire Ebola virus is an essential component of the viral RNA polymerase complex and also functions to antagonize the cellular type I interferon (IFN) response by blocking activation of the transcription factor IRF-3...
  5. Huang Y, Xu L, Sun Y, Nabel G. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell. 2002;10:307-16 pubmed
    ..Expression of the nucleoprotein (NP) and virion-associated proteins VP35 and VP24 led to spontaneous assembly of nucleocapsids in transfected 293T cells by transmission electron microscopy...
  6. Sch mann M, Gantke T, M hlberger E. Ebola virus VP35 antagonizes PKR activity through its C-terminal interferon inhibitory domain. J Virol. 2009;83:8993-7 pubmed publisher
    Ebola virus VP35 contains a C-terminal cluster of basic amino acids required for double-stranded RNA (dsRNA) binding and inhibition of interferon regulatory factor 3 (IRF3)...
  7. Elliott L, Kiley M, McCormick J. Descriptive analysis of Ebola virus proteins. Virology. 1985;147:169-76 pubmed
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  8. M hlberger E, Weik M, Volchkov V, Klenk H, Becker S. Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems. J Virol. 1999;73:2333-42 pubmed
    ..As it was shown previously for MBGV, three of the four EBOV nucleocapsid proteins, NP, VP35, and L, were essential and sufficient for replication...
  9. Bharaj P, Atkins C, Luthra P, Giraldo M, Dawes B, Miorin L, et al. The Host E3-Ubiquitin Ligase TRIM6 Ubiquitinates the Ebola Virus VP35 Protein and Promotes Virus Replication. J Virol. 2017;91: pubmed publisher
    ..The EBOV genome encodes VP35, an important viral protein involved in virus replication by acting as an essential cofactor of the viral ..

More Information

Publications50

  1. Basler C, Mikulasova A, Martinez Sobrido L, Paragas J, Mühlberger E, Bray M, et al. The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3. J Virol. 2003;77:7945-56 pubmed
    The Ebola virus VP35 protein was previously found to act as an interferon (IFN) antagonist which could complement growth of influenza delNS1 virus, a mutant influenza virus lacking the influenza virus IFN antagonist protein, NS1...
  2. Luthra P, Jordan D, Leung D, Amarasinghe G, Basler C. Ebola virus VP35 interaction with dynein LC8 regulates viral RNA synthesis. J Virol. 2015;89:5148-53 pubmed publisher
    Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor...
  3. Leung L, Park M, Martinez O, Valmas C, LOPEZ C, Basler C. Ebolavirus VP35 suppresses IFN production from conventional but not plasmacytoid dendritic cells. Immunol Cell Biol. 2011;89:792-802 pubmed publisher
    ..The VP35 protein impairs RIG-I-like receptor-dependent signaling to inhibit IFN production, and this function has been ..
  4. Theriault S, Groseth A, Neumann G, Kawaoka Y, Feldmann H. Rescue of Ebola virus from cDNA using heterologous support proteins. Virus Res. 2004;106:43-50 pubmed publisher
    ..Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps...
  5. Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, M hlberger E. The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Virology. 2013;441:135-45 pubmed publisher
    ..RNA polymerase (RdRp) complex consists of the catalytic subunit of the polymerase, L, and its cofactor VP35. Using immunofluorescence analysis and coimmunoprecipitation assays, we mapped the VP35 binding site on L...
  6. Volchkov V, Feldmann H, Volchkova V, Klenk H. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998;95:5762-7 pubmed
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  7. Edwards M, Liu G, Mire C, Sureshchandra S, Luthra P, Yen B, et al. Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins. Cell Rep. 2016;14:1632-1640 pubmed publisher
    ..Ebola (EBOV) and Marburg (MARV) viruses each encode a VP35 protein that suppresses RIG-I-like receptor signaling and interferon-α/β (IFN-α/β) production by ..
  8. Bukreyev A, Volchkov V, Blinov V, Netesov S. The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses. FEBS Lett. 1993;322:41-6 pubmed
    ..These fragments were found to encode the VP35 and VP40 proteins. The canonic sequences were revealed before and after each open reading frame...
  9. Kash J, M hlberger E, Carter V, Grosch M, Perwitasari O, Proll S, et al. Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulence. J Virol. 2006;80:3009-20 pubmed publisher
    ..Taken together, these studies showed a correlation between antagonism of type I IFN responses and filovirus virulence...
  10. Haasnoot J, de Vries W, Geutjes E, Prins M, de Haan P, Berkhout B. The Ebola virus VP35 protein is a suppressor of RNA silencing. PLoS Pathog. 2007;3:e86 pubmed publisher
    ..Here, we demonstrate that the Ebola virus VP35 protein is a suppressor of RNAi in mammalian cells and that its RSS activity is functionally equivalent to that of ..
  11. Leung D, Ginder N, Fulton D, Nix J, Basler C, Honzatko R, et al. Structure of the Ebola VP35 interferon inhibitory domain. Proc Natl Acad Sci U S A. 2009;106:411-6 pubmed publisher
    ..The Ebola VP35 protein is multifunctional, acting as a component of the viral RNA polymerase complex, a viral assembly factor, and ..
  12. Johnson R, Bell P, Harty R. Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology. Virol J. 2006;3:31 pubmed publisher
    Recently we described a role for Ebola virus proteins, NP, GP, and VP35 in enhancement of VP40 VLP budding...
  13. Luthra P, Ramanan P, Mire C, Weisend C, Tsuda Y, Yen B, et al. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome. Cell Host Microbe. 2013;14:74-84 pubmed publisher
    ..Here, we show that EBOV VP35 inhibits PACT-induced RIG-I ATPase activity in a dose-dependent manner...
  14. Ren J, Zhang R, Zhang H, Cao X, Liu L, Xie Y. Identification of novel VP35 inhibitors: Virtual screening driven new scaffolds. Biomed Pharmacother. 2016;84:199-207 pubmed publisher
    ..The Ebola virus Viral Protein 35 (VP35) is a multifunctional protein which is critical for virus replication and infection, and it is considered as a ..
  15. Johnson R, McCarthy S, Godlewski P, Harty R. Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles. J Virol. 2006;80:5135-44 pubmed publisher
    ..A mammalian two-hybrid assay and confocal microscopy were used to demonstrate that Ebola virus VP35 and VP40 interact and colocalize in transfected cells...
  16. Volchkov V, Becker S, Volchkova V, Ternovoj V, Kotov A, Netesov S, et al. GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases. Virology. 1995;214:421-30 pubmed
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  17. Watanabe S, Noda T, Kawaoka Y. Functional mapping of the nucleoprotein of Ebola virus. J Virol. 2006;80:3743-51 pubmed
    ..2002) previously demonstrated that NP, together with the minor matrix protein VP24 and polymerase cofactor VP35, is necessary and sufficient for the formation of nucleocapsid-like structures that are morphologically ..
  18. Zhang Y, Ding J, Zhong H, Han J. Exploration micromechanism of VP35 IID interaction and recognition dsRNA: A molecular dynamics simulation. Proteins. 2017;85:1008-1023 pubmed publisher
    Multifunctional viral protein (VP35) encoded by the highly pathogenic Ebola viruses (EBOVs) can antagonize host double-stranded RNA (dsRNA) sensors and immune response because of the simultaneous recognition of dsRNA backbone and blunt ..
  19. Hartman A, Towner J, Nichol S. A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus. Virology. 2004;328:177-84 pubmed publisher
    The ebolavirus VP35 protein antagonizes the cellular type I interferon response by blocking phosphorylation of IRF-3, a transcription factor that turns on the expression of a large number of antiviral genes...
  20. Yen B, Mulder L, Martinez O, Basler C. Molecular basis for ebolavirus VP35 suppression of human dendritic cell maturation. J Virol. 2014;88:12500-10 pubmed publisher
    Zaire ebolavirus (EBOV) VP35 is a double-stranded RNA (dsRNA)-binding protein that inhibits RIG-I signaling and alpha/beta interferon (IFN-α/β) responses by both dsRNA-binding-dependent and -independent mechanisms...
  21. Leung D, Prins K, Borek D, Farahbakhsh M, Tufariello J, Ramanan P, et al. Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35. Nat Struct Mol Biol. 2010;17:165-72 pubmed publisher
    Ebola viral protein 35 (VP35), encoded by the highly pathogenic Ebola virus, facilitates host immune evasion by antagonizing antiviral signaling pathways, including those initiated by RIG-I-like receptors...
  22. Leung D, Ginder N, Nix J, Basler C, Honzatko R, Amarasinghe G. Expression, purification, crystallization and preliminary X-ray studies of the Ebola VP35 interferon inhibitory domain. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:163-5 pubmed publisher
    Ebola VP35 is a multifunctional protein that is important for host immune suppression and pathogenesis. VP35 contains an N-terminal oligomerization domain and a C-terminal interferon inhibitory domain (IID)...
  23. Reid S, C rdenas W, Basler C. Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein. Virology. 2005;341:179-89 pubmed publisher
    We have identified a putative coiled-coil motif within the amino-terminal half of the ebolavirus VP35 protein...
  24. Groseth A, Feldmann H, Theriault S, Mehmetoglu G, Flick R. RNA polymerase I-driven minigenome system for Ebola viruses. J Virol. 2005;79:4425-33 pubmed publisher
  25. Prins K, Binning J, Shabman R, Leung D, Amarasinghe G, Basler C. Basic residues within the ebolavirus VP35 protein are required for its viral polymerase cofactor function. J Virol. 2010;84:10581-91 pubmed publisher
    The ebolavirus (EBOV) VP35 protein binds to double-stranded RNA (dsRNA), inhibits host alpha/beta interferon (IFN-?/?) production, and is an essential component of the viral polymerase complex...
  26. Le Sage V, Cinti A, McCarthy S, Amorim R, Rao S, Daino G, et al. Ebola virus VP35 blocks stress granule assembly. Virology. 2017;502:73-83 pubmed publisher
    ..Moreover we show that EBOV VP35 represses the assembly of canonical and non-canonical SGs induced by a variety of pharmacological stresses...
  27. Zhu Y, Cherukuri N, Jackel J, Wu Z, Crary M, Buckley K, et al. Characterization of the RNA silencing suppression activity of the Ebola virus VP35 protein in plants and mammalian cells. J Virol. 2012;86:3038-49 pubmed publisher
    ..EBOV VP35 is a virulence factor that blocks innate antiviral host responses, including the induction of and response to alpha/..
  28. Volchkov V, Volchkova V, Chepurnov A, Blinov V, Dolnik O, Netesov S, et al. Characterization of the L gene and 5' trailer region of Ebola virus. J Gen Virol. 1999;80 ( Pt 2):355-62 pubmed publisher
    ..The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus...
  29. Dapiaggi F, Pieraccini S, Sironi M. In silico study of VP35 inhibitors: from computational alanine scanning to essential dynamics. Mol Biosyst. 2015;11:2152-7 pubmed publisher
    ..The Ebola virus Viral Protein 35 (VP35) carries out multiple functions necessary for virus replication and infection, in particular interfering with (IFN)-..
  30. Zinzula L, Esposito F, Pala D, Tramontano E. dsRNA binding characterization of full length recombinant wild type and mutants Zaire ebolavirus VP35. Antiviral Res. 2012;93:354-63 pubmed publisher
    The Ebola viruses (EBOVs) VP35 protein is a multifunctional major virulence factor involved in EBOVs replication and evasion of the host immune system...
  31. Takada A, Kawaoka Y. The pathogenesis of Ebola hemorrhagic fever. Trends Microbiol. 2001;9:506-11 pubmed
  32. Subbotina E, Kachko A, Chepurnov A. [The properties of Ebola virus proteins]. Vopr Virusol. 2006;51:4-10 pubmed
    ..It also presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral ..
  33. Volchkov V, Volchkova V, Slenczka W, Klenk H, Feldmann H. Release of viral glycoproteins during Ebola virus infection. Virology. 1998;245:110-9 pubmed publisher
    ..The abundant shedding of soluble GP1 may play an important role in the immunopathology of Ebola hemorrhagic fever in experimentally and naturally infected hosts...
  34. Jin H, Yan Z, Prabhakar B, Feng Z, Ma Y, Verpooten D, et al. The VP35 protein of Ebola virus impairs dendritic cell maturation induced by virus and lipopolysaccharide. J Gen Virol. 2010;91:352-61 pubmed publisher
    ..However, the underlying mechanism remains unclear. In this study, we report that Ebola virus VP35 impairs the maturation of mouse DCs...
  35. Warfield K, Swenson D, Olinger G, Nichols D, Pratt W, Blouch R, et al. Gene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomers. PLoS Pathog. 2006;2:e1 pubmed publisher
    ..A combination of EBOV-specific PMOs targeting sequences of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic regimens...
  36. Prins K, C rdenas W, Basler C. Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1. J Virol. 2009;83:3069-77 pubmed publisher
    The Ebola virus (EBOV) VP35 protein antagonizes the early antiviral alpha/beta interferon (IFN-alpha/beta) response...
  37. Ilinykh P, Lubaki N, Widen S, Renn L, Theisen T, Rabin R, et al. Different Temporal Effects of Ebola Virus VP35 and VP24 Proteins on Global Gene Expression in Human Dendritic Cells. J Virol. 2015;89:7567-83 pubmed publisher
    ..demonstrated that DC maturation is unblocked by disabling the innate response antagonizing domains (IRADs) in EBOV VP35 and VP24 by the mutations R312A and K142A, respectively...
  38. Modrof J, Becker S, M hlberger E. Ebola virus transcription activator VP30 is a zinc-binding protein. J Virol. 2003;77:3334-8 pubmed
  39. Feldmann H, Volchkov V, Volchkova V, Stroher U, Klenk H. Biosynthesis and role of filoviral glycoproteins. J Gen Virol. 2001;82:2839-48 pubmed
  40. Prins K, Delpeut S, Leung D, Reynard O, Volchkova V, Reid S, et al. Mutations abrogating VP35 interaction with double-stranded RNA render Ebola virus avirulent in guinea pigs. J Virol. 2010;84:3004-15 pubmed publisher
    Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-alpha/beta responses that also functions as a viral polymerase cofactor...
  41. Fabozzi G, Nabel C, Dolan M, Sullivan N. Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. J Virol. 2011;85:2512-23 pubmed publisher
    ..In addition to viral protein 35 (VP35), we found that VP30 and VP40 independently act as SRSs. Here, we present the molecular mechanisms of VP30 and VP35...