Gene Symbol: NP
Description: nucleoprotein
Species: Zaire Ebola virus
Products:     NP

Top Publications

  1. Crary S, Towner J, Honig J, Shoemaker T, Nichol S. Analysis of the role of predicted RNA secondary structures in Ebola virus replication. Virology. 2003;306:210-8 pubmed
  2. Watanabe S, Noda T, Kawaoka Y. Functional mapping of the nucleoprotein of Ebola virus. J Virol. 2006;80:3743-51 pubmed
    At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. Huang et al. (Y...
  3. Kiley M, Cox N, Elliott L, Sanchez A, deFries R, Buchmeier M, et al. Physicochemical properties of Marburg virus: evidence for three distinct virus strains and their relationship to Ebola virus. J Gen Virol. 1988;69 ( Pt 8):1957-67 pubmed
    ..Peptide mapping of glycoproteins, nucleoproteins (NP) and viral structural protein (VP40) demonstrated extensive sequence conservation in the proteins of viruses ..
  4. Noda T, Watanabe S, Sagara H, Kawaoka Y. Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus. J Virol. 2007;81:3554-62 pubmed
    Expression of Ebola virus nucleoprotein (NP) in mammalian cells leads to the formation of helical structures, which serve as a scaffold for the nucleocapsid...
  5. Feldmann H, Volchkov V, Volchkova V, Stroher U, Klenk H. Biosynthesis and role of filoviral glycoproteins. J Gen Virol. 2001;82:2839-48 pubmed
  6. Licata J, Johnson R, Han Z, Harty R. Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles. J Virol. 2004;78:7344-51 pubmed
    ..budding assay and cotransfection experiments to examine the contributions of the glycoprotein (GP), nucleoprotein (NP), and VP24 of Ebola virus in facilitating release of VP40 VLPs...
  7. Huang Y, Xu L, Sun Y, Nabel G. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell. 2002;10:307-16 pubmed
    ..Expression of the nucleoprotein (NP) and virion-associated proteins VP35 and VP24 led to spontaneous assembly of nucleocapsids in transfected 293T cells ..
  8. Theriault S, Groseth A, Neumann G, Kawaoka Y, Feldmann H. Rescue of Ebola virus from cDNA using heterologous support proteins. Virus Res. 2004;106:43-50 pubmed publisher
    ..Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps...
  9. Maruyama T, Rodriguez L, Jahrling P, Sanchez A, Khan A, Nichol S, et al. Ebola virus can be effectively neutralized by antibody produced in natural human infection. J Virol. 1999;73:6024-30 pubmed
    ..Antibodies reactive with nucleoprotein (NP), envelope glycoprotein (GP), and secreted envelope glycoprotein (sGP) were characterized by immunofluorescence and ..

More Information


  1. Volchkov V, Volchkova V, Dolnik O, Feldmann H, Klenk H. Polymorphism of filovirus glycoproteins. Adv Virus Res. 2005;64:359-81 pubmed
  2. Goh G, Dunker A, Uversky V. Detection of links between Ebola nucleocapsid and virulence using disorder analysis. Mol Biosyst. 2015;11:2337-44 pubmed publisher
    ..Comparison of the percentage of disorder (PID) in nucleocapsid proteins VP30 and NP reveals high correlation between nucleocapsid PIDs and the case-fatality rates of EBOV...
  3. Volchkov V, Feldmann H, Volchkova V, Klenk H. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998;95:5762-7 pubmed
    ..As a result of these observations, it should now be considered that proteolytic processing of GP may be an important determinant for the pathogenicity of Ebola virus...
  4. Noda T, Hagiwara K, Sagara H, Kawaoka Y. Characterization of the Ebola virus nucleoprotein-RNA complex. J Gen Virol. 2010;91:1478-83 pubmed publisher
    When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically...
  5. Boehmann Y, Enterlein S, Randolf A, M hlberger E. A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire. Virology. 2005;332:406-17 pubmed publisher
    ..This system was used to exchange the EBOV Zaire and EBOV Reston nucleocapsid (NC) proteins NP, VP35, VP30, and L, which catalyze replication and transcription...
  6. Kobinger G, Feldmann H, Zhi Y, Schumer G, Gao G, Feldmann F, et al. Chimpanzee adenovirus vaccine protects against Zaire Ebola virus. Virology. 2006;346:394-401 pubmed publisher
    ..These results validate further development of Chimpanzee-based vaccine and highlight the impact of pre-existing immunity to the vaccine carrier...
  7. Bukreyev A, Volchkov V, Blinov V, Netesov S. The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses. FEBS Lett. 1993;322:41-6 pubmed
    ..It is suggested that these sequences are mRNA extremities and at the same time the regulatory elements for mRNA transcription. Homology between the MBG and EBO proteins was discovered. ..
  8. Kash J, M hlberger E, Carter V, Grosch M, Perwitasari O, Proll S, et al. Global suppression of the host antiviral response by Ebola- and Marburgviruses: increased antagonism of the type I interferon response is associated with enhanced virulence. J Virol. 2006;80:3009-20 pubmed publisher
    ..Taken together, these studies showed a correlation between antagonism of type I IFN responses and filovirus virulence...
  9. Elliott L, Kiley M, McCormick J. Descriptive analysis of Ebola virus proteins. Virology. 1985;147:169-76 pubmed
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  10. Saijo M, Niikura M, Morikawa S, Ksiazek T, Meyer R, Peters C, et al. Enzyme-linked immunosorbent assays for detection of antibodies to Ebola and Marburg viruses using recombinant nucleoproteins. J Clin Microbiol. 2001;39:1-7 pubmed publisher
    The full-length nucleoprotein (NP) of Ebola virus (EBO) was expressed as a His-tagged recombinant protein (His-EBO-NP) by a baculovirus system...
  11. M hlberger E, Weik M, Volchkov V, Klenk H, Becker S. Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems. J Virol. 1999;73:2333-42 pubmed
    ..As it was shown previously for MBGV, three of the four EBOV nucleocapsid proteins, NP, VP35, and L, were essential and sufficient for replication...
  12. Xu L, Sanchez A, Yang Z, Zaki S, Nabel E, Nichol S, et al. Immunization for Ebola virus infection. Nat Med. 1998;4:37-42 pubmed
    ..We analyzed immune responses to the viral nucleoprotein (NP) and the secreted or transmembrane forms of the glycoprotein (sGP or GP) and their ability to protect against ..
  13. Johnson R, Bell P, Harty R. Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology. Virol J. 2006;3:31 pubmed publisher
    Recently we described a role for Ebola virus proteins, NP, GP, and VP35 in enhancement of VP40 VLP budding...
  14. Volchkov V, Becker S, Volchkova V, Ternovoj V, Kotov A, Netesov S, et al. GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases. Virology. 1995;214:421-30 pubmed
    ..Our data indicate that the editing site in the Ebola virus glycoprotein gene is recognized not only by Ebola virus polymerase but also by DNA-dependent RNA polymerases of different origin...
  15. Basler C, Wang X, Muhlberger E, Volchkov V, Paragas J, Klenk H, et al. The Ebola virus VP35 protein functions as a type I IFN antagonist. Proc Natl Acad Sci U S A. 2000;97:12289-94 pubmed
    ..The Ebola virus VP35 therefore is likely to inhibit induction of type I IFN in Ebola virus-infected cells and may be an important determinant of Ebola virus virulence in vivo. ..
  16. Volchkov V, Volchkova V, Chepurnov A, Blinov V, Dolnik O, Netesov S, et al. Characterization of the L gene and 5' trailer region of Ebola virus. J Gen Virol. 1999;80 ( Pt 2):355-62 pubmed publisher
    ..The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus...
  17. Martin J, Sullivan N, Enama M, Gordon I, Roederer M, Koup R, et al. A DNA vaccine for Ebola virus is safe and immunogenic in a phase I clinical trial. Clin Vaccine Immunol. 2006;13:1267-77 pubmed publisher
  18. Niikura M, Ikegami T, Saijo M, Kurane I, Miranda M, Morikawa S. Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. J Clin Microbiol. 2001;39:3267-71 pubmed
    ..enzyme-linked immunosorbent assay (ELISA) system using a novel monoclonal antibody (MAb) to the nucleoprotein (NP). This antibody recognized an epitope defined by a 26-amino-acid stretch near the C terminus of NP...
  19. Groseth A, Feldmann H, Theriault S, Mehmetoglu G, Flick R. RNA polymerase I-driven minigenome system for Ebola viruses. J Virol. 2005;79:4425-33 pubmed publisher
  20. Ebihara H, Takada A, Kobasa D, Jones S, Neumann G, Theriault S, et al. Molecular determinants of Ebola virus virulence in mice. PLoS Pathog. 2006;2:e73 pubmed publisher
    ..These findings suggest a critical role for overcoming the interferon-induced antiviral state in the pathogenicity of EBOV and offer new insights into the pathogenesis of EBOV infection...
  21. Volchkov V, Volchkova V, Slenczka W, Klenk H, Feldmann H. Release of viral glycoproteins during Ebola virus infection. Virology. 1998;245:110-9 pubmed publisher
    ..The abundant shedding of soluble GP1 may play an important role in the immunopathology of Ebola hemorrhagic fever in experimentally and naturally infected hosts...
  22. Subbotina E, Kachko A, Chepurnov A. [The properties of Ebola virus proteins]. Vopr Virusol. 2006;51:4-10 pubmed
    ..It also presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral ..
  23. Hartlieb B, Weissenhorn W. Filovirus assembly and budding. Virology. 2006;344:64-70 pubmed publisher
    ..As a consequence, assembly and budding steps are defined to specific cellular compartments, and the recent progress in understanding how the different components are assembled into stable enveloped virus particles is reviewed...
  24. Niikura M, Ikegami T, Saijo M, Kurata T, Kurane I, Morikawa S. Analysis of linear B-cell epitopes of the nucleoprotein of ebola virus that distinguish ebola virus subtypes. Clin Diagn Lab Immunol. 2003;10:83-7 pubmed
    ..We developed monoclonal antibodies (MAbs) to the nucleoprotein (NP) of Ebola virus Zaire subtype and analyzed their cross-reactivities to the Reston and Sudan subtypes...
  25. Modrof J, Becker S, M hlberger E. Ebola virus transcription activator VP30 is a zinc-binding protein. J Virol. 2003;77:3334-8 pubmed