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Genomes and GenesSpecies | YKU70SummaryGene Symbol: YKU70 Description: Yku70p Alias: HDF1, NES24 Species: Top Publications
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Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeresDiego Bonetti
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
PLoS Genet 6:e1000966. 2010..Thus, chromosome end degradation is controlled by telomeric proteins that specifically inhibit the action of different nucleases...- In Saccharomyces cerevisiae, yKu and subtelomeric core X sequences repress homologous recombination near telomeres as part of the same pathwayMarcus E Marvin
Institute of Genetics, University of Nottingham, Medical School, Queens Medical Centre, Nottingham, UK
Genetics 183:441-51, 1SI-12SI. 2009..Furthermore, core X bestowed this protection as part of the same pathway as yKu. Our findings implicate a role for both yKu and core X in stabilizing the genome against recombination events involving telomeric sequences... - Regulation of telomere length by fatty acid elongase 3 in yeast. Involvement of inositol phosphate metabolism and Ku70/80 functionSuriyan Ponnusamy
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Biol Chem 283:27514-24. 2008..to determine downstream mechanisms, epistasis experiments were performed, and data indicated that ELO3 and YKU70/80 share a common pathway for the regulation of telomere length... - Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaksEun Yong Shim
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
EMBO J 29:3370-80. 2010..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation... - Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damageS E Lee
Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Cell 94:399-409. 1998..b>hdf1 cells, lacking Ku70p, fail to escape from this RAD9/RAD17-dependent checkpoint... - Sequential loading of Saccharomyces cerevisiae Ku and Cdc13p to telomeresTzung Ju Wu
Institute of Biopharmaceutical Science and Institute of Biochemistry and Molecular Biology, National Yang Ming University, Shih Pai, Taipei, Taiwan, ROC
J Biol Chem 284:12801-8. 2009..Our results also offer a mechanism that the binding of Cdc13p to telomeres might prevent Yku from initiating DNA double-stranded break repair pathway on telomeres... - A short C-terminal domain of Yku70p is essential for telomere maintenanceL Driller
Institute for Biochemistry, University of Munich LMU, Feodor Lynen Str 25, D 81377 Munich, Germany
J Biol Chem 275:24921-7. 2000..the role of the Yku70p subunit in these two different pathways, we generated C-terminal deletions of the Yku70 protein and examined their ability to complement the phenotypes of a yku70(-) strain... - The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA endsDiego Bonetti
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
PLoS ONE 5:e14142. 2010..Thus, resection of DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity... - Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiaeG T Milne
Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Mol Cell Biol 16:4189-98. 1996..Ku8O associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells... - Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functionsAlbert Ribes-Zamora
Department of Pediatrics, Hematology Oncology Section, Baylor College of Medicine, One Baylor Plaza, BCM225, Houston, Texas 77030 3411, USA
Nat Struct Mol Biol 14:301-7. 2007..We propose a 'two-face' model for Ku and that divergent evolution of these faces allowed Ku's dual role in NHEJ and telomere maintenance... - Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeastS G Martin
Swiss Institute for Experimental Cancer Research, Epalinges
Cell 97:621-33. 1999..Consistently, yKu- or SIR-deficient strains are hypersensitive to DNA-damaging agents. The release of yKu from telomeric chromatin may allow efficient scanning of the genome for DNA strand breaks... - De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaksWei Zhang
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Genes Dev 24:502-15. 2010..These studies therefore identify a mechanism by which the ATR family of kinases enforces genome integrity, and a process that underscores the contribution of Cdc13 to the fate of DNA ends... - Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomeraseLeticia R Vega
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Genet 3:e105. 2007..These data suggest that wild-type levels of telomere-bound telomerase are critical for the viability of strains whose telomeres are already susceptible to degradation... - The association of yKu with subtelomeric core X sequences prevents recombination involving telomeric sequencesMarcus E Marvin
Institute of Genetics, University of Nottingham, Medical School, Queen s Medical Centre, Nottingham, UK
Genetics 183:453-67, 1SI-13SI. 2009..We believe our findings support our hypothesis that yKu and core X play a pivotal role in maintaining genome stability through nuclear architecture by mediating a defensive fold-back structure at yeast chromosome ends... - Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome endsAnne E Stellwagen
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genes Dev 17:2384-95. 2003..Thus, the interaction between Ku and TLC1 RNA enables telomerase to act at both broken and normal chromosome ends... - Suppression of gross chromosomal rearrangements by yKu70-yKu80 heterodimer through DNA damage checkpointsSoma Banerjee
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:1816-21. 2006..To understand the mechanism of Ku as a genome gatekeeper, we overexpressed the yKu70-yKu80 heterodimer and monitored the formation of GCRs... - Cell cycle-dependent regulation of yeast telomerase by KuTimothy S Fisher
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nat Struct Mol Biol 11:1198-205. 2004..These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase... - Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1Avgi Tsolou
Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
DNA Repair (Amst) 6:1607-17. 2007..Our observations show that Mrc1, a core component of the replication fork, plays an important role in telomere capping, protecting from nucleases and checkpoint pathways... - The Yku70-Yku80 complex contributes to regulate double-strand break processing and checkpoint activation during the cell cycleMichela Clerici
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milan, Italy
EMBO Rep 9:810-8. 2008..Here, we show that a lack of any of the NHEJ proteins Yku (Yku70-Yku80), Lif1 or DNA ligase IV (Dnl4) increases 5' DSB end degradation in G1 phase, with ykuDelta cells showing the .. - Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomeraseNathalie Grandin
UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
Mol Genet Genomics 277:685-99. 2007..Here, we report that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence... - EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutantsLaura Maringele
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
Genes Dev 16:1919-33. 2002..Therefore, subsets of both DNA-damage and spindle checkpoint pathways cooperate to regulate cell division of yku70Delta mutants... - The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activitiesR M Polotnianka
Department of Biochemistry, Tulane University Medical Center, New Orleans, Louisiana 70112, USA
Curr Biol 8:831-4. 1998..Furthermore, mutations in HDF1 or HDF2 rapidly reduce telomeric poly (TG1-3) tract size [1-3], hinting also at a possible telomeric function of Ku... - Telomere capping in non-dividing yeast cells requires Yku and Rap1Momchil D Vodenicharov
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
EMBO J 29:3007-19. 2010..However, Yku and Rap1 become crucially important for this function in these cells. After inactivation of Yku70 in G1-arrested cells, moderate but significant telomere degradation occurs... - Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2Eleni P Mimitou
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
EMBO J 29:3358-69. 2010..radiation sensitivity of a mutant defective for extensive resection (exo1? sgs1?) cannot be suppressed by the yku70? mutation, indicating that Ku suppression is specific to the initiation of resection... - Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiaeK Myung
Ludwig Institute for Cancer Research, University of California San Diego, 92093, USA
Nature 411:1073-6. 2001..Mutations that inactivate these pathways cause high rates of GCRs and show synergistic interactions, indicating that the pathways that suppress GCRs all compete for the same DNA substrates... - Telomere maintenance is dependent on activities required for end repair of double-strand breaksC I Nugent
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Biol 8:657-60. 1998..Ku, Cdc13 and telomerase define three epistasis groups required in parallel for telomere maintenance... - Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutantS H Teo
Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
EMBO Rep 2:197-202. 2001..In Saccharomyces cerevisiae, deletion of YKU70 or YKU80 results in an inability to grow at 37 degrees C... - A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulatorMichael Downey
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
Cell 124:1155-68. 2006..Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation... - Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitmentN Grandin
Ecole Normale Superieure, UMR CNRS ENS 5665, Lyon 69364, France
Mol Cell Biol 20:8397-408. 2000..depended on telomerase (Est2/TLC1) and Est1, a direct regulator of telomerase, but also on the yeast Ku proteins, yKu70/Hdf1 and yKu80/Hdf2, that have been previously implicated in DNA repair and telomere maintenance... - Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencingS J Boulton
Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
EMBO J 17:1819-28. 1998..These data provide important insights into DNA DSB repair and the linkage of this process to telomere length homeostasis and transcriptional silencing... - A domain of Rad9 specifically required for activation of Chk1 in budding yeastRichard T Blankley
School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
J Cell Sci 117:601-8. 2004..This work extends data showing that separable domains within the Rad9 adaptor protein allow it to activate two distinct kinase signalling pathways independently of each other... - Regulation of nuclear positioning and dynamics of the silent mating type loci by the yeast Ku70/Ku80 complexKerstin Bystricky
Laboratoire de Biologie Moleculaire Eucaryote, Bâtiment IBCG, Universite de Toulouse, Toulouse, France
Mol Cell Biol 29:835-48. 2009..However, HMLalpha, unlike HMRa and most telomeres, shows increased NE association in a strain lacking yeast Ku70 (yKu70)... - The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiaeNathalie Grandin
Ecole Normale Superieure de Lyon, UMR CNRS 5665, 69364 Lyon, France
Mol Cell Biol 23:3721-34. 2003..required for type II recombination) in cdc13-1 yku70delta or yku70delta tlc1delta mutants, but also in cdc13-1 YKU70(+) tlc1delta mutants, still permitted type II recombination, but this process was now entirely dependent on the .. - Telomere instability caused by subtelomeric Y' amplification and rearrangements in Saccharomyces cerevisiae (ku70 tel1 and ku70 rad50) double mutantsMiguel J Ruiz-Gómez
Radiobiological Institute, University of Munich Schillerstr 42, 80336 Munich, Germany
Indian J Exp Biol 49:324-31. 2011Telomeres solve the end-replication problem. Previous results suggested a relation between Yku70/80 and proteins Tell and Rad50 in telomere stabilization... - The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylationHui Yong Lian
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK
Mol Biol Cell 22:1753-65. 2011..Genome-wide analysis shows that regions extending up to 80 kb from telomeres replicate abnormally early in a yku70 mutant... - SUMOylation regulates telomere length homeostasis by targeting Cdc13Lisa E Hang
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:920-6. 2011..cdc13-snm's effect on telomere length is epistatic with stn1, but not with yku70, tel1 or est1 alleles, and is suppressed by Stn1 overexpression... - Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiaeMatthew Jessulat
Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont, Canada K1S 5B6
Arch Biochem Biophys 469:157-64. 2008..We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination... - Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joiningKihoon Lee
Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
Genetics 176:2003-14. 2007..Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity... - The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expressionStefanie E Grund
Biochemie Zentrum der Universitat Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 182:897-910. 2008..Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes... - Est1 protects telomeres and inhibits subtelomeric y'-element recombinationXia Jing Tong
The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
Mol Cell Biol 31:1263-74. 2011..Our results demonstrate that one major functional role that Est1 brings to the telomerase complex is the capping or protection of telomeres... - Inhibition of DNA double-strand break repair by the Ku heterodimer in mrx mutants of Saccharomyces cerevisiaeBrian M Wasko
Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA
DNA Repair (Amst) 8:162-9. 2009..experiments demonstrated that the 5'-end of TLC1 RNA was essential and a segment containing a binding site for the Yku70/Yku80 complex was sufficient for suppression... - Yeast Nej1 is a key participant in the initial end binding and final ligation steps of nonhomologous end joiningXi Chen
Radiation Oncology Research Laboratory, Department of Radiation Oncology, and the Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Biol Chem 286:4931-40. 2011.... - Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncappingJames M Dewar
Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle upon Tyne, Tyne and Wear, UK
EMBO J 29:4020-34. 2010..Thus, attenuation of the DDR at uncapped telomeres can circumvent the need for otherwise-essential telomere capping proteins... - Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replicationFei Long Meng
The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China
EMBO J 28:1466-78. 2009..Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo... - Yeast silent mating type loci form heterochromatic clusters through silencer protein-dependent long-range interactionsAdriana Miele
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA, USA
PLoS Genet 5:e1000478. 2009.... - The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiaeCatherine L Vandre
Department of Cell and Developmental Biology, University of Illinois, Urbana, Illinois 61801, USA
Genetics 180:1407-18. 2008..Here, we tested whether Ku contributes to silencing at HML or HMR. Mutant analysis revealed that yKu70 and Sir1 act collectively to silence the mating-type genes at HML and HMR... - Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathwaysS J Boulton
Wellcome CRC Institute, Cambridge, UK
EMBO J 15:5093-103. 1996..However, yku70 mutations enhance the radiosensitivity of rad52 strains, which are deficient in homologous recombination... - Histone H4 lysine 91 acetylation a core domain modification associated with chromatin assemblyJianxin Ye
Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
Mol Cell 18:123-30. 2005..These results indicate an important role for histone modifications outside the NH2-tail domains in the processes of chromatin assembly, DNA repair, and transcriptional silencing... - Multiple pathways promote short-sequence recombination in Saccharomyces cerevisiaeGlenn M Manthey
Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 E Duarte Road, Duarte, CA 91010 0269, USA
Mol Cell Biol 22:5347-56. 2002..Additional evidence suggests that SSR is distinct from nonhomologous end joining and is superimposed upon basal homologous recombination... - The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaksEun Yong Shim
Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
Mol Cell Biol 25:3934-44. 2005..Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p... - The yeast histone chaperone chromatin assembly factor 1 protects against double-strand DNA-damaging agentsJeffrey Linger
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Aurora, CO 80045, USA
Genetics 171:1513-22. 2005..We propose that CAF-1 has an essential role in assembling chromatin during double-strand-DNA repair... - The Est1 subunit of Saccharomyces cerevisiae telomerase makes multiple contributions to telomere length maintenanceSara K Evans
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Genetics 162:1101-15. 2002..The isolation of mutations that perturb separate functions of Est1 demonstrates that a telomerase holoenzyme subunit can contribute multiple regulatory roles to telomere length maintenance... - DNA polymerase 4 of Saccharomyces cerevisiae is important for accurate repair of methyl-methanesulfonate-induced DNA damageCatherine H Sterling
Department of Therapeutic Radiology and Genetics, Yale University, New Haven, Connecticut 06510, USA
Genetics 172:89-98. 2006..The MMS sensitivity of a Pol4-deficient strain can be rescued by deletion of YKu70. We also show that deletion of Pol4 results in a 6- to 14-fold increase in the MMS-induced mutation frequency and .. - Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complexYves Corda
Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
Mol Cell Biol 25:10652-64. 2005..We report that deletion of YKU70 or YKU80 suppresses mec1Delta, but not rad53Delta, lethality... - Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathwayT E Wilson
Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 274:23599-609. 1999..Pol4 is thus specifically recruited to perform gap-filling in an NHEJ pathway that must also involve as yet unidentified nucleases... - The NoCut pathway links completion of cytokinesis to spindle midzone function to prevent chromosome breakageCaren Norden
Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland
Cell 125:85-98. 2006..We propose that NoCut monitors clearance of chromatin from the midzone to ensure that cytokinesis completes only after all chromosomes have migrated to the poles... - Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeastD A Thrower
Department of Biology, CB3280 University of North Carolina, Chapel Hill, NC 27599 3280, USA
Mol Biol Cell 12:2800-12. 2001..In cells deleted for yKU70, yKU80, or SIR2, a 10-kb region of the dicentric chromosome stretched along the spindle axis to a length of 6 .. - A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viabilityChristine Soustelle
Commissaritat à l Energy Atomique, UMR 217 CNRS CEA, DSV DRR, Fontenay aux Roses, France
Mol Cell Biol 24:5130-43. 2004..These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway... - Different mating-type-regulated genes affect the DNA repair defects of Saccharomyces RAD51, RAD52 and RAD55 mutantsMaria Valencia-Burton
Department of Biology and Resenstiel Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Genetics 174:41-55. 2006..All three recombination-defective mutations are made more sensitive by deletions of Rad6 and of the histone deacetylases Rpd3 and Ume6, although these mutations are not themselves CPT or phleomycin sensitive... - Roles of nonhomologous end-joining pathways in surviving topoisomerase II-mediated DNA damageMobeen Malik
Department of Molecular Pharmacology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105, USA
Mol Cancer Ther 5:1405-14. 2006..Using this approach, we found that yku70 (hdf1), yku80 (hdf2), and other genes required for NHEJ were important for cell survival following exposure to .. - Effects of HDF1 (Ku70) and HDF2 (Ku80) on spontaneous and DNA damage-induced intrachromosomal recombination in Saccharomyces cerevisiaeT Cervelli
Genetica e Biochimica Tossicologica, Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy
Mol Gen Genet 264:56-63. 2000..b>HDF1 and HDF2, which have been identified in Saccharomyces cerevisiae as homologues of the Ku70 and Ku80 proteins of .. - Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiaeY Tsukamoto
Department of Molecular Biology, Institute of Medical Science, University of Tokyo, Japan
Nature 388:900-3. 1997..One of the factors involved in the end-joining process is Hdf1, a yeast homologue of Ku protein... - Multiple roles for Saccharomyces cerevisiae histone H2A in telomere position effect, Spt phenotypes and double-strand-break repairHolly R Wyatt
Interdisciplinary Program in Molecular and Cellular Biology, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
Genetics 164:47-64. 2003..In the absence of the DSB and telomere-binding protein yKu70, the bleomycin sensitivity of hta1tpe alleles is further enhanced... - Dual functions of Mdt1 in genome maintenance and cell integrity pathways in Saccharomyces cerevisiaeAna Traven
St Vincent s Institute of Medical Research and Department of Medicine SVH, University of Melbourne, Fitzroy, Victoria 3065, Australia
Yeast 27:41-52. 2010..Overall, the results indicate that Mdt1 has partially separable functions in both cell wall and genome integrity pathways... - NuA4 subunit Yng2 function in intra-S-phase DNA damage responseJohn S Choy
Department of Molecular Genetics and Cell Biology Center for Molecular Oncology, University of Chicago, Chicago, Illinois 60637, USA
Mol Cell Biol 22:8215-25. 2002..Our results implicate nucleosomal histone acetylation in maintaining genomic integrity during chromosomal replication... - Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeastV Hegde
Department of Biochemistry and Kaplan Cancer Center, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
Nucleic Acids Res 28:2779-83. 2000..However, NHEJ of blunt ends, while very inefficient, is not further reduced by mutations in YKU70, SIR2, SIR3, SIR4 or SRS2, suggesting that this rejoining process occurs by a different mechanism. - Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeastKunheng Luo
Department of Biological Chemistry, UCLA School of Medicine, 90095, USA
Genes Dev 16:1528-39. 2002..Several proteins are involved in telomeric heterochromatin structure including Rap1, Sir2, Sir3, Sir4, yKu70 (Hdf1), yKu80 (Hdf2), and the N termini of histones H3 and H4... - New function of CDC13 in positive telomere length regulationB Meier
Institute for Biochemistry, University of Munich LMU, D 81377 Munich, Germany
Mol Cell Biol 21:4233-45. 2001..In a synthetic lethality screen with YKU70, the 70-kDa subunit of the telomere-associated Yku heterodimer, we identified a new mutation in CDC13, cdc13-4, .. - Suppression of homologous recombination by the Saccharomyces cerevisiae linker histoneJessica A Downs
The Wellcome Trust Cancer Research UK Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
Mol Cell 11:1685-92. 2003..Finally, we show that Hho1p is inhibitory to the recombination-dependent mechanism of telomere maintenance. The role of linker histones in genome stability, aging, and tumorigenesis is discussed... - Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequencesJia Lin Ma
Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
Mol Cell Biol 23:8820-8. 2003..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation... - Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replicationSanchita Krishna
Department of Molecular Genetics and Microbiology and Cancer Research and Treatment Center, University of New Mexico School of Medicine, Albuquerque, NM 87131, United States
DNA Repair (Amst) 6:797-808. 2007..BIR is also elevated in rad51 mutants, but yku70Delta did not suppress BIR in a rad51 background. These results indicate that Mre11 functions in Rad51-independent BIR, and that Ku functions in Rad51-dependent BIR... - Telomere-related functions of yeast KU in the repair of bleomycin-induced DNA damageAngela T Y Tam
St Vincent s Institute of Medical Research, 9 Princes Street, Fitzroy, VIC 3065, Australia
Biochem Biophys Res Commun 357:800-3. 2007..cerevisiae, DSBs are predominantly repaired by RAD52-dependent homologous recombination (HR) with some support by Yku70/Yku80 (KU)-dependent pathways... - Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processesBenoit Palancade
Institut Curie, Centre de Recherche, and Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, F 75248 Paris, France
Mol Biol Cell 18:2912-23. 2007..for the establishment of the appropriate sumoylation of several cellular proteins, including the DNA repair factor Yku70. Moreover, restoration of nuclear envelope-associated Ulp1 in nucleoporin mutants reestablishes proper sumoylation .. - Mdt1 facilitates efficient repair of blocked DNA double-strand breaks and recombinational maintenance of telomeresBrietta L Pike
St Vincent s Institute of Medical Research, 9 Princes Street, Fitzroy, VIC 3065, Australia
Mol Cell Biol 27:6532-45. 2007.... - The Ku complex in silencing the cryptic mating-type loci of Saccharomyces cerevisiaeErin E Patterson
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706 1532, USA
Genetics 180:771-83. 2008..Additional ChIP experiments provided evidence that Ku functioned directly at the HM loci. Thus Ku and Sir1 had overlapping roles in silencing the HM loci... - Regulation of homologous integration in yeast by the DNA repair proteins Ku70 and RecQYoshimasa Yamana
Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2 4 Hibikino, Wakamatsu ku, Kitakyushu, 808 0196, Japan
Mol Genet Genomics 273:167-76. 2005..We present evidence for a genetic interaction between SGS1 and YKU70, which encodes the S. cerevisiae homologue of the human DNA helicase Ku70... - Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1Jacqueline Franke
Zentrum für Medizinische Biotechnologie, Universitat Duisburg Essen, Universitätsstr 5, 45117 Essen, Germany
J Cell Sci 121:3553-60. 2008..Furthermore, tgs1Delta cells displayed a shortened replicative lifespan, suggesting that the loss of the m(3)G cap of TLC1 causes premature aging... - The functional importance of telomere clustering: global changes in gene expression result from SIR factor dispersionAngela Taddei
Friedrich Miescher Institute for Biomedical Research and National Center for Competence in Research Frontiers in Genetics, CH 4058 Basel, Switzerland
Genome Res 19:611-25. 2009..This demonstrates that patterns of gene expression can be regulated by changing the spatial distribution of repetitive DNA sequences that bind repressive factors... - Telomerase, Ku, and telomeric silencing in Saccharomyces cerevisiaeS K Evans
Verna and Marrs MacLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA
Chromosoma 107:352-8. 1998.... - Genetic analysis of ionizing radiation-induced mutagenesis in Saccharomyces cerevisiae reveals TransLesion Synthesis (TLS) independent of PCNA K164 SUMOylation and ubiquitinationClark C Chen
Department of Neurosurgery, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA
DNA Repair (Amst) 5:1475-88. 2006..A genetic model based on these observations is proposed... - Nuclear pore complexes in the organization of silent telomeric chromatinV Galy
Laboratoire de Biologie Cellulaire du Noyau, Institut Pasteur, CNRS URA 1773, Paris, France
Nature 403:108-12. 2000..The only protein found to be involved in the peripheral localization of telomeres is Yku70/Yku80... - The ability of Sgs1 to interact with DNA topoisomerase III is essential for damage-induced recombinationAyako Ui
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
DNA Repair (Amst) 4:191-201. 2005.... - A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organizationXiaolan Zhao
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:4777-82. 2005..The substrates for this SUMO ligase include a subunit of the octameric complex, Smc5, and the DNA repair protein Yku70. We further show that the abolition of the SUMO E3 activity of Mms21 leads to such disparate phenotypes as DNA .. - Dynamic regulation of single-stranded telomeres in Saccharomyces cerevisiaeStephanie Smith
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 178:693-701. 2008..Therefore, the yKu70-80 heterodimer and telomerase maintain telomere size, and the helicase activity of Pif1 likely also helps to .. - The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombinationW Siede
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235, USA
Genetics 142:91-102. 1996..In the yeast Saccharomyces cerevisiae, we have examined the phenotype conferred by a deletion of HDF1, the putative homologue of the 70-kD subunit of the DNA-end binding Ku complex of DNA-PK...
Research Grants
- Etiology of Chromosome TranslocationsSang Eun Lee; Fiscal Year: 2010..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2010..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2009..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2007..Since the components of DSB repair are conserved from yeast to humans, the insights garnered from our research will be valuable for dissecting the equivalent process in human cells and will be of relevance to public health. ..
- Mechanisms of Nonhomologous Repair of Damaged DNASang Eun Lee; Fiscal Year: 2005..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
- Etiology of Chromosome TranslocationsSang Eun Lee; Fiscal Year: 2009..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..