nqrB

Summary

Gene Symbol: nqrB
Description: Na(+)-translocating NADH-quinone reductase subunit B
Species: Vibrio vulnificus CMCP6

Top Publications

  1. ncbi Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 363:75-7. 1995
  2. ncbi Recent progress in the Na(+)-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    Biochim Biophys Acta 1505:37-44. 2001
  3. ncbi FMN is covalently attached to a threonine residue in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba, Japan
    FEBS Lett 488:5-8. 2001
  4. ncbi Covalently bound flavin in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    FEBS Lett 474:165-8. 2000
  5. ncbi Na(+) translocation by bacterial NADH:quinone oxidoreductases: an extension to the complex-I family of primary redox pumps
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1505:45-56. 2001
  6. ncbi Inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-hydroxyquinoline N-oxide and Ag+ toward the Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biol Pharm Bull 22:1064-7. 1999
  7. ncbi Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 422:240-2. 1998
  8. ncbi Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus
    P Beattie
    Institute for Cell and Molecular Biology, Edinburgh University, UK
    FEBS Lett 356:333-8. 1994
  9. ncbi Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 356:330-2. 1994
  10. ncbi Sequencing and preliminary characterization of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi
    W Zhou
    Department of Biochemistry, University of Illinois at Urbana-Champaign 61801, USA
    Biochemistry 38:16246-52. 1999

Scientific Experts

Detail Information

Publications10

  1. ncbi Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 363:75-7. 1995
    ..FEBS Lett. 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex...
  2. ncbi Recent progress in the Na(+)-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    Biochim Biophys Acta 1505:37-44. 2001
    ..There are conflicting results on the existence of other flavin cofactors. Recent studies revealed that the NqrB and NqrC subunits have a covalently bound flavin, possibly FMN, which is attached to a specified threonine residue...
  3. ncbi FMN is covalently attached to a threonine residue in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba, Japan
    FEBS Lett 488:5-8. 2001
    ..We previously demonstrated that both NqrB and NqrC subunits contain a flavin cofactor covalently attached to a threonine residue...
  4. ncbi Covalently bound flavin in the NqrB and NqrC subunits of Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, 263 8522, Chiba, Japan
    FEBS Lett 474:165-8. 2000
    ..On SDS-PAGE of the purified complex, NqrB and NqrC subunits were found to give yellow-green fluorescent bands under UV illumination...
  5. ncbi Na(+) translocation by bacterial NADH:quinone oxidoreductases: an extension to the complex-I family of primary redox pumps
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1505:45-56. 2001
    ..alginolyticus, higher (Na(+) or H(+)) transport stoichiometries are expected for complex I, suggesting the presence of a second coupling site...
  6. ncbi Inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-hydroxyquinoline N-oxide and Ag+ toward the Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biol Pharm Bull 22:1064-7. 1999
    ..The site of Ag+ inhibition was the initial reaction of the NQR complex catalyzed by Nqr6 subunit. The time courses of Ag+ inhibition and the release of FAD indicate that the Ag+-denatured Nqr6 subunit gradually releases FAD...
  7. ncbi Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus
    Y Nakayama
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 422:240-2. 1998
    ..By modifying the detection method of proteins on SDS-PAGE, we could detect all six subunits encoded by NQR operon in the purified NQR complex. The open reading frame of each subunit was identified from its N-terminal amino acid sequence...
  8. ncbi Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus
    P Beattie
    Institute for Cell and Molecular Biology, Edinburgh University, UK
    FEBS Lett 356:333-8. 1994
    ..From sequence comparisons, we conclude that the Na(+)-dependent NADH-ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H(+)-dependent enzymes of both prokaryotes and eukaryotes...
  9. ncbi Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 356:330-2. 1994
    ..Among the subclones selected by probe C, the expression of the beta-subunit as a gene product was detected in Escherichia coli membranes by activity staining and Western blotting...
  10. ncbi Sequencing and preliminary characterization of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi
    W Zhou
    Department of Biochemistry, University of Illinois at Urbana-Champaign 61801, USA
    Biochemistry 38:16246-52. 1999
    ..alginolyticus and Haemophilus influenzae. Homology studies show that a number of other bacteria, including a number of pathogenic species, also have an Na(+)-NQR operon...