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Genomes and Genes | UBC13SummaryGene Symbol: UBC13 Description: Ubc13p Species: Top Publications
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Publications
Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugationPhilipp Stelter
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
Nature 425:188-91. 2003..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability...- The NEF4 complex regulates Rad4 levels and utilizes Snf2/Swi2-related ATPase activity for nucleotide excision repairKerrington L Ramsey
Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
Mol Cell Biol 24:6362-78. 2004..Moreover, the E2s Ubc9 and Ubc13 were linked to the NEF4 repair pathway by genetic criteria... - Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5Joanne L Parker
Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Herts, UK
EMBO J 28:3657-66. 2009..the mechanism of poly-ubiquitylation of the replication clamp PCNA by two cooperating E2-E3 pairs, Rad6-Rad18 and Ubc13-Mms2-Rad5... - Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiaeVenkateswarlu Gangavarapu
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, TX 77555 1061
Mol Cell Biol 26:7783-90. 2006..PRR) of discontinuities that form in the newly synthesized DNA opposite from DNA lesions, mediated by the Mms2-Ubc13 ubiquitin-conjugating enzyme and Rad5... - Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2pEfterpi Papouli
Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
Mol Cell 19:123-33. 2005..Our findings suggest a mechanism by which SUMO and ubiquitin cooperatively control the choice of pathway for the processing of DNA lesions during replication... - Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formationMichael J Eddins
Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Nat Struct Mol Biol 13:915-20. 2006..We have determined the crystal structure of an Mms2-Ubc13-ubiquitin (UEV-E2-Ub) covalent intermediate with donor ubiquitin linked to the active site residue of Ubc13... - Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repairHelle D Ulrich
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
J Biol Chem 278:7051-8. 2003The RING finger protein RAD5 interacts and cooperates with the UBC13-MMS2 ubiquitin-conjugating enzyme in postreplication DNA damage repair in yeast... - The srs2 suppressor of UV sensitivity acts specifically on the RAD5- and MMS2-dependent branch of the RAD6 pathwayH D Ulrich
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
Nucleic Acids Res 29:3487-94. 2001..A sub-branch within the RAD6 pathway is mediated by RAD5, UBC13 and MMS2, and a comprehensive analysis of the srs2 effect on other known members of the RAD6 pathway reported here .. - Noncovalent interaction between ubiquitin and the human DNA repair protein Mms2 is required for Ubc13-mediated polyubiquitinationS McKenna
Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 276:40120-6. 2001..enzyme variant that is involved in the error-free DNA postreplicative repair pathway through its association with Ubc13, an E2... - Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repairR M Hofmann
Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
Cell 96:645-53. 1999..We show that Mms2p forms a specific heteromeric complex with the UBC13-encoded E2 and is required for the Ubc13p-dependent assembly of polyubiquitin chains linked through lysine 63... - Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5Candice M Carlile
Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
J Biol Chem 284:29326-34. 2009..ubiquitin moiety of monoubiquitinated proliferating cell nuclear antigen (monoUb-PCNA) by the E2-E3 complex of (Ubc13-Mms2)-Rad5. This promotes error-free bypass of DNA damage lesions... - Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repairH D Ulrich
Department of Molecular Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
EMBO J 19:3388-97. 2000Two ubiquitin-conjugating enzymes, RAD6 and the heteromeric UBC13-MMS2 complex, have been implicated in post-replicative DNA damage repair in yeast. Here we provide a mechanistic basis for cooperation between the two enzymes... - The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathwaysW Xiao
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E5 Canada
Genetics 155:1633-41. 2000..Our data also suggest that MMS2 and UBC13 play a key role in coordinating the response of the error-free subpathways; Mms2 and Ubc13 form a complex required .. - In vitro assembly and recognition of Lys-63 polyubiquitin chainsR M Hofmann
Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
J Biol Chem 276:27936-43. 2001..We showed previously that the products of the UBC13 and MMS2 genes function in error-free post-replicative DNA repair in the yeast Saccharomyces cerevisiae and form a .. - Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimerA P VanDemark
Department of Biophysics and Biophysical Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
Cell 105:711-20. 2001..The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites .. - UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiaeJ Brusky
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
Curr Genet 37:168-74. 2000The Ubc13 protein was recently identified for its unique role in ubiquitin (Ub) chain assembly at the Ub Lys-63 residue instead of the conventional Lys-48 residue... - The roles of PCNA SUMOylation, Mms2-Ubc13 and Rad5 in translesion DNA synthesis in Saccharomyces cerevisiaeAgnieszka Halas
Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02 106 Warsaw, Poland
Mol Microbiol 80:786-97. 2011Mms2, in concert with Ubc13 and Rad5, is responsible for polyubiquitination of replication processivity factor PCNA. This modification activates recombination-like DNA damage-avoidance mechanisms, which function in an error-free manner... - The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Pol?/Rev1-dependent translesion DNA synthesisNeeru M Sharma
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
J Biol Chem 286:33557-66. 2011..The new mode of Rev1-PCNA binding described here suggests a mechanism by which Rev1 adopts a catalytically inactive configuration at the replication fork... - Constitutive fusion of ubiquitin to PCNA provides DNA damage tolerance independent of translesion polymerase activitiesLandon Pastushok
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada
Nucleic Acids Res 38:5047-58. 2010..As expected, the DNA damage resistance provided by PCNA.Ub is not dependent on RAD18 or UBC13. Surprisingly, inactivation of TLS polymerases did not abolish PCNA.Ub resistance to DNA damage, nor did PCNA... - The RAD6 DNA damage tolerance pathway operates uncoupled from the replication fork and is functional beyond S phaseGeorgios I Karras
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Cell 141:255-67. 2010..We therefore propose that the RAD6 pathway acts on single-stranded gaps left behind newly restarted replication forks... - Ubiquitin-dependent DNA damage bypass is separable from genome replicationYasukazu Daigaku
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
Nature 465:951-5. 2010..Our approach has revealed the distribution of PRR tracts in a synchronized cell population. It will allow an in-depth mechanistic analysis of how cells manage the processing of lesions to their genomes during and after replication... - Post-replication repair suppresses duplication-mediated genome instabilityChristopher D Putnam
Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
PLoS Genet 6:e1000933. 2010..Here, we found that the RAD6- and RAD18-dependent post-replication repair (PRR) and the RAD5-, MMS2-, UBC13-dependent error-free PRR branch acted in concert with the replication stress checkpoint to suppress duplication-.. - Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damageAdelina A Davies
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
Cell 141:1080-7. 2010..Instead, our findings suggest that ubiquitylation of the 9-1-1 complex may be a background reaction that in some cases can mediate proteasomal degradation... - The role of replication bypass pathways in dicentric chromosome formation in budding yeastAndrew L Paek
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
Genetics 186:1161-73. 2010..Fourth, by studying genes implicated in suppression of GCRs in other studies, we found that inverted repeat fusion has a profile of genetic regulation distinct from these other major forms of GCR formation... - E2 Polyubiquitin-conjugating enzyme Ubc13 in keratinocytes is essential for epidermal integrityKoji Sayama
Department of Dermatology, Ehime University Graduate School of Medicine, Ehime 791 0295, Japan
J Biol Chem 285:30042-9. 2010The E2 polyubiquitin-conjugating enzyme Ubc13 is a mediator of innate immune reactions. Ubc13 mediates the conjugation of keratin (K)63-linked polyubiquitin chains onto TNF receptor-associated factor 6 and IKK? during NF-?B activation... - RAD18 transmits DNA damage signalling to elicit homologous recombination repairJun Huang
Department of Therapeutic Radiology, Yale University School of Medicine, P O Box 208040, New Haven, CT 06520, USA
Nat Cell Biol 11:592-603. 2009..Together, our data uncovers RAD18 as a key factor that orchestrates HRR through surveillance of the DNA damage signal... - A role for histone H2B during repair of UV-induced DNA damage in Saccharomyces cerevisiaeEmmanuelle M D Martini
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Genetics 160:1375-87. 2002..When combined with a ubc13Delta mutation, which is also epistatic with rad5Delta, the htb1-3 mutation enhanced UV-induced cell killing. These results suggest that histone H2B acts in a novel RAD5-dependent branch of PRR... - Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage responseBryan M O'Neill
Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Nucleic Acids Res 32:6519-30. 2004..Tof1 is known to be involved in stabilizing stalled replication forks and our data suggest that Wss1 and Psy2 similarly function to stabilize or process stalled or collapsed replication forks... - Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1AParker L Andersen
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
J Cell Biol 170:745-55. 2005b>Ubc13, a ubiquitin-conjugating enzyme (Ubc), requires the presence of a Ubc variant (Uev) for polyubiquitination... - The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent mannerShuhua Chen
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse D 35043, Marburg, Germany
Nucleic Acids Res 33:5878-86. 2005.... - Ubiquitin-dependent protein degradationM Hochstrasser
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
Annu Rev Genet 30:405-39. 1996..This diversity underlies both the high substrate specificity of the ubiquitin system and the variety of regulatory mechanisms that it serves... - Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymesHiroyuki Hiraishi
Bio Oriented Technology Research Advancement Institution BRAIN, Japan
Biosci Biotechnol Biochem 70:2762-5. 2006..cerevisiae cells overexpressing essential ubiquitin ligase Rsp5 or ubiquitin-conjugating enzymes (Ubc1-Ubc13) showed tolerance to various stresses... - Requirement of Nse1, a subunit of the Smc5-Smc6 complex, for Rad52-dependent postreplication repair of UV-damaged DNA in Saccharomyces cerevisiaeSergio R Santa Maria
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 301 University Blvd, Galveston, TX 77555 1061, USA
Mol Cell Biol 27:8409-18. 2007In Saccharomyces cerevisiae, postreplication repair (PRR) of UV-damaged DNA occurs by a Rad6-Rad18- and an Mms2-Ubc13-Rad5-dependent pathway or by a Rad52-dependent pathway... - Arabidopsis UEV1D promotes Lysine-63-linked polyubiquitination and is involved in DNA damage responseRui Wen
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5
Plant Cell 20:213-27. 2008..The ubiquitin-conjugating enzyme Ubc13 and the Ubc enzyme variant (Uev) methyl methanesulfonate2 (Mms2) are required for this process... - Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitinationGreta L Loring
Department of Genetics and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
Cell Cycle 7:96-105. 2008..In contrast, Ubc13/Mms2 is required for G(2) delay and does not contribute to Chf protein turnover... - The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNAIrene Saugar
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, UK
Nucleic Acids Res 40:245-57. 2012..Our identification of Mgs1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambivalent role of the protein in damage processing... - The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activityTatsuya Ii
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, USA
Cell Cycle 6:2800-9. 2007..not its individual subunits, stimulates several human and yeast Ub conjugating enzymes, including Ubc1, 4, 5, and Ubc13-Mms2...
Research Grants
- Translesion synthesis by yeast DNA polymerasesLouise Prakash; Fiscal Year: 2007....
- Translesion DNA synthesis in humansLouise Prakash; Fiscal Year: 2007..Our understanding of the manner in which human cells overcome blocks to DNA replication should be greatly enhanced by these studies. ..
- Bypass of DNA lesions by translesion synthesis in yeastLouise Prakash; Fiscal Year: 2010....
- REPAIR OF DNA DAMAGED BY UV IRRADIATION IN YEASTLouise Prakash; Fiscal Year: 1999..Bypass replication of templates carrying a site specific UV lesion will be reconstituted using purified components. ..
- REPAIR OF DNA DAMAGED BY UV IRRADIATION IN YEASTLouise Prakash; Fiscal Year: 1993..Phosphorylation of REV1, REV3, and RAD6 proteins by the CDC7 encoded serine(threonine) protein kinase, which is involved in UV mutagenesis, will be examined...
- REPAIR OF DNA DAMAGED BY UV IRRADIATION IN YEASTLouise Prakash; Fiscal Year: 2003..The proposed studies will identify by genetic means the other components of the MMS2-UBC13 dependent error-free postreplicational repair...
- Translesion DNA synthesis in humansLouise Prakash; Fiscal Year: 2010..The proposed studies will elucidate the roles of human DNA polymerases in promoting replication through a variety of DNA lesions and they will examine the mechanisms of this process. ..