Genomes and Genes
Gene Symbol: tufA
Description: protein chain elongation factor EF-Tu (duplicate of tufB)
Alias: ECK3326, JW3301, kirT, pulT
- Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decodingJared M Schrader
Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
Proc Natl Acad Sci U S A 108:5215-20. 2011..Consistent with available crystal structures, the identity of the esterified amino acid and three base pairs in the T stem of tRNA combine to define the affinity of each aa-tRNA for EF-Tu, both off and on the ribosome...
- The role of an activating peptide in protease-mediated suicide of Escherichia coli K12Nikki A Copeland
Department of Biology, The University of York, Heslington, York YO10 5YW, UK
J Biol Chem 280:112-7. 2005..coli K-12 by Lit is a variant form of "cofactor-induced activation," a mechanism of protease activation that has only been documented previously in pathogen subversion of mammalian hemostasis cascades...
- Conformational changes of the small ribosomal subunit during elongation factor G-dependent tRNA-mRNA translocationFrank Peske
Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
J Mol Biol 343:1183-94. 2004....
- Substrate-assisted catalysis of peptide bond formation by the ribosomeJoshua S Weinger
Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
Nat Struct Mol Biol 11:1101-6. 2004..These results suggest that substrate assistance has been retained as a catalytic strategy during the evolution of the prebiotic peptidyl transferase center into the modern ribosome...
- Mutagenesis of glutamine 290 in Escherichia coli and mitochondrial elongation factor Tu affects interactions with mitochondrial aminoacyl-tRNAs and GTPase activitySenyene Eyo Hunter
Department of Chemistry, University of North Carolina, Campus Box 3290, Chapel Hill, North Carolina 27599 3290, USA
Biochemistry 43:6917-27. 2004..Surprisingly, while E. coli EF-Tu Q290L is more active in polymerization with mitochondrial Phe-tRNA(Phe), this variant has low activity in the formation of a stable ternary complex with mitochondrial aa-tRNA...
- Water induced effects on the thermal response of a proteinSimone Melchionna
Dipartimento di Fisica and INFM, Universita La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Phys Rev Lett 92:158101. 2004..The results support the notion that the dynamics of vicinal water makes a substantial contribution to the activity optimum of proteins...
- Determination of the kinetics of guanine nucleotide exchange on EF-Tu and EF-Ts: continuing uncertaintiesKeith L Manchester
School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, South Africa
Biochem Biophys Res Commun 314:1-5. 2004..Metabolic control analysis has been applied to determine the degree of flux control of the different steps in the pathway...
- Regulation of translation of the head protein of T4 bacteriophage by specific binding of EF-Tu to a leader sequenceLarry Snyder
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824 4320, USA
J Mol Biol 334:349-61. 2003....
- Effects of mutagenesis of Gln97 in the switch II region of Escherichia coli elongation factor Tu on its interaction with guanine nucleotides, elongation factor Ts, and aminoacyl-tRNATomas Navratil
Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
Biochemistry 42:13587-95. 2003..Strikingly, the Q97P variant was completely inactive in ternary complex formation, accounting for its inability to function in polymerization. The structural basis of these observations is discussed...
- Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopyMikel Valle
Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
Nat Struct Biol 10:899-906. 2003..From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome...
- Essential role of histidine 84 in elongation factor Tu for the chemical step of GTP hydrolysis on the ribosomeTina Daviter
Institute of Physical Biochemistry, University of Witten Herdecke, Stockumer Strasse 10, 58448, Witten, Germany
J Mol Biol 332:689-99. 2003..We propose that the catalytic role of His84 is to stabilize the transition state of GTP hydrolysis by hydrogen bonding to the attacking water molecule or, possibly, the gamma-phosphate group of GTP...
- Functional EF-Tu with large C-terminal extensions in an E coli strain with a precise deletion of both chromosomal tuf genesRobert Schnell
Department of Microbiology, Stockholm University, S 106 91 Stockholm, Sweden
FEBS Lett 538:139-44. 2003An Escherichia coli strain was constructed in which both chromosomal genes encoding elongation factor (EF)-Tu (tufA and tufB) have been inactivated with precise coding sequence replacements...
- Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infectionCarsten Struve
Department of Gastrointestinal Infections, Statens Serum Institut, 2300 Copenhagen S, Denmark
Microbiology 149:167-76. 2003..Transposon insertion sites in attenuated mutants were, among others, in genes encoding well-known K. pneumoniae virulence factors such as lipopolysaccharide and capsule, as well as in genes of unknown function...
- Specificity of elongation factor EF-TU for hydrophobic peptidesAbdelharim Malki
Stress Molecules, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France
Biochem Biophys Res Commun 296:749-54. 2002..Our results suggest that EF-Tu, like DnaK, interacts albeit more weakly with the hydrophobic regions of substrate protein and are consistent with the hypothesis that it possesses chaperone properties...
- Mechanism of elongation factor (EF)-Ts-catalyzed nucleotide exchange in EF-Tu. Contribution of contacts at the guanine baseHans Joachim Wieden
Institute of Physical Biochemistry, University of Witten Herdecke, 58448 Witten, Germany
J Biol Chem 277:6032-6. 2002....
- Evolution of a molecular switch: universal bacterial GTPases regulate ribosome functionC E Caldon
School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia
Mol Microbiol 41:289-97. 2001..If this is the case, then a corollary of this hypothesis is that GTPases that do not bind RNA arose at a later time from an RNA-binding progenitor that lost the capability to bind RNA...
- Mapping the interaction of SmpB with ribosomes by footprinting of ribosomal RNANatalia Ivanova
Department of Cell and Molecular Biology, BMC, Uppsala University Box 596, S 75 124 Uppsala, Sweden
Nucleic Acids Res 33:3529-39. 2005....
- Opposite roles of domains 2+3 of Escherichia coli EF-Tu and Bacillus stearothermophilus EF-Tu in the regulation of EF-Tu GTPase activityHana Sanderová
Department of Gene Expression, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nam 2, 166 37 Prague 6, Czech Republic
Biochim Biophys Acta 1752:11-7. 2005..This can be explained by a stabilization effect of domains 2+3 on alpha-helical regions of the G-domain as revealed by CD spectroscopy...
- The identification of the determinants of the cyclic, sequential binding of elongation factors tu and g to the ribosomeHuijun Yu
Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA
J Mol Biol 386:802-13. 2009....
- Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysisElizabeth Villa
Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 106:1063-8. 2009..Our data suggest that GTP hydrolysis on EF-Tu is controlled through a hydrophobic gate mechanism...
- Mutants of the RNA-processing enzyme RNase E reverse the extreme slow-growth phenotype caused by a mutant translation factor EF-TuDisa L Hammarlöf
Microbiology Programme, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
Mol Microbiol 70:1194-209. 2008..In some cases (n= 7) suppression was due to mutations in tufA but, surprisingly, in most cases (n= 73) to mutations in rne, the gene coding for RNase E...
- Conserved discrimination against misacylated tRNAs by two mesophilic elongation factor Tu orthologsTerry J T Cathopoulis
Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
Biochemistry 47:7610-6. 2008..These results also demonstrate that the capacity of EF-Tu to discriminate against both of these aminoacyl-tRNAs is conserved even in bacteria like E. coli that do not generate either misacylated tRNA...
- Abundant and diverse bacteria involved in DMSP degradation in marine surface watersErinn C Howard
Departments of Microbiology, University of Georgia, Athens, GA, USA
Environ Microbiol 10:2397-410. 2008....
- Changes in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable stateAlicia Muela
Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco, Bilbao, Spain
FEMS Microbiol Ecol 64:28-36. 2008..However, proteins exclusive to the VBNC state were not detected...
- Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis systemYoshio Doi
Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan
J Am Chem Soc 129:14458-62. 2007..L-1-Pyrenylalanine, L-2-pyrenylalanine, and DL-2-anthraquinonylalanine, which are hardly or only slightly incorporated with the wild-type EF-Tu, were successfully incorporated into a protein using these EF-Tu mutants...
- Nitrosative stress treatment of E. coli targets distinct set of thiol-containing proteinsNicolas Brandes
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA
Mol Microbiol 66:901-14. 2007..Of the 10 proteins that we identified, six (AtpA, AceF, FabB, GapA, IlvC, TufA) have been shown to harbour functionally important thiol groups and are encoded by genes that are considered ..
- The 51-63 base pair of tRNA confers specificity for binding by EF-TuLee E Sanderson
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
RNA 13:835-40. 2007..While this contact is an important specificity determinant, it is clear that others remain to be identified...
- Molecular complementarity between tetracycline and the GTPase active site of elongation factor TuSusan E Heffron
Department of Physiology and Biophysics, 346 D Med Sci I, University of California, Irvine, CA 92697 4560, USA
Acta Crystallogr D Biol Crystallogr 62:1392-400. 2006....
- Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunitHiroshi Kita
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan
J Biosci Bioeng 101:421-6. 2006..The fused protein, a single-chain alpha-less Qbeta replicase, was mostly found in the soluble fraction and could be readily purified. These results pave the way for the large-scale production of the highly purified form of this enzyme...
- The importance of P-loop and domain movements in EF-Tu for guanine nucleotide exchangeLouise D Dahl
Department of Molecular Biology, Aarhus University, Denmark
J Biol Chem 281:21139-46. 2006..The mutations had no effect on EF-Tu-dependent delivery of aminoacyl-tRNA to the ribosome...
- Solving the structure of Escherichia coli elongation factor Tu using a twinned data setSusan E Heffron
University of California, Irvine, USA
Acta Crystallogr D Biol Crystallogr 62:433-8. 2006..The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP...
- The tmRDB and SRPDB resourcesEbbe Sloth Andersen
Department of Molecular Biology, University of Aarhus, C F Moellers Alle, Building 139, 8000 Aarhus, Denmark
Nucleic Acids Res 34:D163-8. 2006..All alignments can be easily examined using a new exploratory browser. The databases provide links to high-resolution structures and serve as depositories for structures obtained by molecular modeling...
- The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: target proteins identifiedReinhard Depping
AG Molekulare Genetik, Ruhr Universitat Bochum, Bochum, Germany
Biochem Biophys Res Commun 335:1217-23. 2005..E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication...
- Structure of an EF-Tu complex with a thiazolyl peptide antibiotic determined at 2.35 A resolution: atomic basis for GE2270A inhibition of EF-TuS E Heffron
Department of Physiology, University of California, Irvine 92697 4560, USA
Biochemistry 39:37-45. 2000..The results presented here provide the details necessary to enhance the solubility of GE2270A without disrupting its inhibitory properties...
- Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscLC Berrier
Laboratoire des Biomembranes, UMR CNRS 8619, Universite Paris Sud, 91405 Orsay Cedex, France
J Bacteriol 182:248-51. 2000..Release of these proteins is not observed in the absence of a Tris-EDTA treatment which disrupts the outer membrane, indicating that, in intact cells, they are transferred to the periplasm upon shock, presumably through the MscL channel...
- Crystal structure of intact elongation factor EF-Tu from Escherichia coli in GDP conformation at 2.05 A resolutionH Song
School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
J Mol Biol 285:1245-56. 1999..Comparisons of the Mg2+ binding site with other guanine nucleotide binding proteins in GDP-bound forms show that the Mg2+ co-ordination patterns are well preserved among these structures...
- The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequenceR A Laursen
J Biol Chem 256:8102-9. 1981..Structural features of the aminoacyl-tRNA binding site are discussed in the light of sequence and other chemical and biochemical data...
- The nucleotide sequence of the cloned tufA gene of Escherichia coliT Yokota
Gene 12:25-31. 1980The 4 kb (8.5 % lambda units) EcoRI fragment harboring the tufA gene of Escherichia coli was cloned using plasmid pTUA1 (Shibuya et al., 1979) and its structure was analyzed...
- DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coliL E Post
Cell 15:215-29. 1978..Extensive sequence similarity between the str and spc promoter regions is found downstream from the Pribnow box-that is, in a transcribed region preceding the translation start sites...
- A secondary promoter for elongation factor Tu synthesis in the str ribosomal protein operon of Escherichia coliJ M Zengel
Mol Gen Genet 185:487-92. 1982..These results indicate that there is a secondary promoter for EF-Tu expression, apparently located within the structural gene for elongation factor EF-G...
- Evidence for an internal promoter preceding tufA in the str operon of Escherichia coliG An
J Bacteriol 149:548-53. 1982We constructed plasmids carrying tufA from which the major promoter for the rpsL-rpsG-fus-tufA operon (also called the str operon) had been removed...
- A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory proteinY W Hwang
New York State Office of Mental Retardation and Developmental Disabilities, Staten Island, New York 10314
J Biol Chem 262:13081-5. 1987..substitution (Asp to Asn) at position 138 of Escherichia coli elongation factor Tu (EF-Tu) was introduced in the tufA gene clone by oligonucleotide site-directed mutagenesis. The mutated tufA gene was then expressed in maxicells...
- Site-directed mutagenesis of the GDP binding domain of bacterial elongation factor TuY W Hwang
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314
Arch Biochem Biophys 274:394-403. 1989....
- Mutagenesis of bacterial elongation factor Tu at lysine 136. A conserved amino acid in GTP regulatory proteinsY W Hwang
Molecular Biology Department, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314
J Biol Chem 264:8304-9. 1989..These results indicate that EF-Ts is essential to the growth of E. coli and suggest a technique for studying EF-Ts mutants as well as for identifying other guanine nucleotide exchange enzymes...
- Mapping of two promoters for elongation factor Tu within the structural gene for elongation factor GJ M Zengel
Department of Biology, University of Rochester, NY 14627
Biochim Biophys Acta 1050:317-22. 1990..transcription rates from various regions of the str operon in cells carrying the fus gene and the beginning of the tufA gene on a high copy number plasmid confirmed that transcription was initiated within a 600 bp EcoRI fragment in the ..
- Elongation factor Tu is methylated in response to nutrient deprivation in Escherichia coliC C Young
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802
J Bacteriol 173:3096-100. 1991..We propose that the nutrient-dependent methylation of EF-Tu may be involved in the regulation of growth, possibly as a principal component of an unidentified signal transduction pathway in bacteria...
- Elongation factor Tu: a molecular switch in protein biosynthesisA Weijland
SDI No 61840 du CNRS, Laboratoire de Biochimie, Ecole Polytechnique, Palaiseau, France
Mol Microbiol 6:683-8. 1992..These studies have emphasized the manifold aspects of the mechanisms regulating the activity of EF-Tu in the bacterial cell...
- Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coliU Johanson
Department of Molecular Biology, Uppsala University, Sweden
Gene 120:93-8. 1992..The order of conservation at the nt and aa level is rpsL greater than tufA greater than rpsG greater than f usA. A striking difference is that the rpsG-encoded ribosomal protein, S7, in E...
- Location of the site of methylation in elongation factor TuR A Laursen
FEBS Lett 107:359-62. 1979
- Transcription of the E. coli tufB gene: cotranscription with four tRNA genes and inhibition by guanosine-5'-diphosphate-3'-diphosphateA Miyajima
Mol Gen Genet 183:13-9. 1981..The synthesis of this mRNA molecule is completely abolished by low concentrations of ppGpp. Neither pppGpp, ppGp, nor pGpp was effective as inhibitor in this cell-free system...
- Protecting the neighborhood: extreme measuresS Gottesman
National Cancer Institute, Building 37, Room 2E18, Bethesda, MD 20892-4255, USA
Proc Natl Acad Sci U S A 95:2731-2. 1998
- Mutational analysis of the roles of residues in Escherichia coli elongation factor Ts in the interaction with elongation factor TuY Zhang
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
J Biol Chem 273:4556-62. 1998..Mutations prepared in EF-Tu indicate that His-19 and Gln-114 but not Glu-348 in EF-Tu are moderately important for its interaction with EF-Ts...
- Isolation of tRNA isoacceptors by affinity chromatography with immobilized elongation factor Tu from Escherichia coliG Chinali
Dipartimento de Medicina Clinica e Sperimentale, Facolta di Medicina e Chirurgia, Universita di Napoli Federico II, Italy
J Biochem Biophys Methods 34:1-10. 1997..kirromycin allowed the isolation of the aminoacylated tRNA from bulk tRNA. A rapid and nearly quantitative recovery of highly purified tRNA isoacceptors for various amino acids was obtained in one chromatographic step...
- Specific peptide-activated proteolytic cleavage of Escherichia coli elongation factor TuT Georgiou
School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
Proc Natl Acad Sci U S A 95:2891-5. 1998..This system apparently represents an activation mechanism for proteolysis that targets one of nature's most evolutionarily conserved proteins for site-specific cleavage...
- The G222D mutation in elongation factor Tu inhibits the codon-induced conformational changes leading to GTPase activation on the ribosomeE Vorstenbosch
Leiden Institute of Chemistry, Department of Biochemistry, Leiden University, The Netherlands
EMBO J 15:6766-74. 1996..Increasing the Mg2+ concentration appears to overcome the inhibition by screening the negative charges...
- Interplay of methionine tRNAs with translation elongation factor Tu and translation initiation factor 2 in Escherichia coliJ M Guillon
Laboratoire de Biochimie, URA CNRS 1970, Ecole Polytechnique, F91128 Palaiseau Cedex, France
J Biol Chem 271:22321-5. 1996..M., Mechulam, Y., Schmitter, J.-M., Blanquet, S., and Fayat, G. (1992) J. Bacteriol. 174, 4294-4301...
- Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coliB Kraal
Leiden Institute of Chemistry, Department of Biochemistry, Leiden University, The Netherlands
Biochem Cell Biol 73:1167-77. 1995..mutagenesis methods have yielded sets of purified mutant EF-Tu resistant to kirromycin (kirT) or pulvomycin (pulT)...
- Purification of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharoseT D Caldas
Biochimie Génétique, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, Paris Cedex 05, 75251, France
Protein Expr Purif 14:65-70. 1998..The specific reactivities of the elongation factors with thiol-Sepharose allow their efficient purification and suggest that they possess hitherto undiscovered properties connected with their reactive thiols...
- The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coliJ R Mesters
Department of Biochemistry, Leiden University, The Netherlands
EMBO J 13:4877-85. 1994..The first inhibits access of the antibiotic to its binding site on EF-Tu.GTP. A second mechanism exists on the ribosome, when mutant EF-Tu species release kirromycin and polypeptide chain elongation continues...
- Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNAO Wiborg
Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus C, Denmark
J Biol Chem 271:20406-11. 1996..Their functional roles are discussed in relation to the structure of elongation factor Tu in complex with aminoacyl-tRNA...
- The structure of the Escherichia coli EF-Tu.EF-Ts complex at 2.5 A resolutionT Kawashima
European Molecular Biology Laboratory, Grenoble Outstation, France
Nature 379:511-8. 1996..The interaction of EF-Ts with EF-Tu results principally in the disruption of the Mg2+ ion binding site, thereby reducing the affinity of EF-Tu for guanine nucleotides...
- Prokaryotic elongation factor Tu is phosphorylated in vivoC Lippmann
Institut fur Biochemie, Freie Universitat Berlin, Germany
J Biol Chem 268:601-7. 1993..coli factor but could also be demonstrated for Thermus thermophilus HB8 EF-Tu. Overexpression of tufA did not increase the number of EF-Tu molecules to be phosphorylated...
- Substitution of proline 82 by threonine induces autophosphorylating activity in GTP-binding domain of elongation factor TuR H Cool
Laboratoire de Biochimie, Ecole Polytechnique, URA 240 du Centre National de la Recherche Scientifique, Palaiseau, France
J Biol Chem 265:6744-9. 1990..These results are in agreement with the observations derived from x-ray diffraction analysis that the tertiary structure of the GTP-binding domain of elongation factor Tu and that of p21 are similar...
- Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesisA R Timms
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Great Britain
Mol Gen Genet 232:89-96. 1992..One possibility is that spontaneous mutation may often occur in bursts when an error correction mechanism (eg., proofreading, mismatch correction) is temporarily inactive.(ABSTRACT TRUNCATED AT 250 WORDS)..
- The complete amino-acid sequence of elongation factor Tu from Escherichia coliM D Jones
Eur J Biochem 108:507-26. 1980..The lysine residue at positon 56, in the polypeptide chain is partly methylated. The C-terminal residue is a mixture of serine and glycine, and this was the only heterogeneity found in the EF-Tu preparation used in this study...
- Mutants of Escherichia coli altered in both genes coding for the elongation factor TuJ A van de Klundert
Proc Natl Acad Sci U S A 75:4470-3. 1978..If the mutant gene is the tufA gene, the seisitive cells can be made resistant through inactivation of the tufB gene by insertion of the ..
- The ribosome as affinity matrix': efficient purification scheme for translation factorsM C Ganoza
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
Biochimie 78:51-61. 1996..The procedure markedly simplifies the isolation, in homogeneous form, of all the non-ribosomal proteins required to reconstruct translation...
- Refined structure of elongation factor EF-Tu from Escherichia coliM Kjeldgaard
Department of Chemistry, Aarhus University, Denmark
J Mol Biol 223:721-42. 1992..Elongation factor Tu interacts with proteins, nucleic acids and nucleotides, making this molecule well suited as a model system for the study of these interactions...
- Trans-translation: the tmRNA-mediated surveillance mechanism for ribosome rescue, directed protein degradation, and nonstop mRNA decayDaniel Dulebohn
Department of Biochemistry and Cell Biology and The Center for Infectious Diseases, Stony Brook University, Stony Brook, New York 11794, USA
Biochemistry 46:4681-93. 2007..This review will focus on recent advances in our understanding of the structural properties, mechanistic details, and physiological significance of this unique RNA and its principal protein partners...
- Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAsSenyene Eyo Hunter
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
RNA Biol 1:95-102. 2004..coli Phe-tRNA.(Phe) These data suggest that the residue at position 287 plays an important role in the binding and EF-Tu-mediated delivery of mitochondrial aa-tRNAs to the A-site of the ribosome...
- Time-dependent translational response of E. coli to excess Zn(II)J Allen Easton
Department of Chemistry and Biochemistry, 160 Hughes Hall, Miami University, Oxford, OH 45056, USA
J Biomol Tech 17:303-7. 2006..the levels of OmpF, AspC, YcdO, Eno, and CysE increased after 30 min of Zn(II) stress, while the levels of Tig, TufA, SelA, and LeuC decreased relative to non-stressed controls...
- Proteomic approach to identification of proteins reactive for abasic sites in DNARobert A Rieger
Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794, USA
Mol Cell Proteomics 5:858-67. 2006..We report identification of seven proteins from Escherichia coli (AroF, DnaK, MutM, PolA, TnaA, TufA, and UvrA) and two proteins from bakers' yeast (ARC1 and Ygl245wp) reactive for AP sites in this system.
- Thermostability of multidomain proteins: elongation factors EF-Tu from Escherichia coli and Bacillus stearothermophilus and their chimeric formsHana Sanderová
Department of Protein Biosynthesis, Institute of Molecular Genetics, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic
Protein Sci 13:89-99. 2004..It was lower in the GTP conformation, and the mechanism involved the destabilization of the alpha-helical regions of the G-domain by Ecdomain 2...
- Regulation of the expression of tufA and tufB, the two genes coding for the elongation factor EF-Tu in Escherichia coliP H van der Meide
FEBS Lett 139:325-30. 1982