tRNA guanine transglycosylase

Summary

Gene Symbol: tRNA guanine transglycosylase
Description: queuine tRNA-ribosyltransferase
Species:

Top Publications

  1. ncbi Cloning and molecular characterization of the DNA ligase gene (lig) from Zymomonas mobilis
    K B Shark
    School of Biological Sciences, University of Nebraska, Lincoln 68588 0118
    FEMS Microbiol Lett 75:19-26. 1992
  2. ncbi Crystal structures of tRNA-guanine transglycosylase (TGT) in complex with novel and potent inhibitors unravel pronounced induced-fit adaptations and suggest dimer formation upon substrate binding
    Bernhard Stengl
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 370:492-511. 2007
  3. ncbi Crystallographic study of inhibitors of tRNA-guanine transglycosylase suggests a new structure-based pharmacophore for virtual screening
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 338:55-75. 2004
  4. ncbi Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    Chembiochem 4:1066-77. 2003
  5. ncbi Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate
    Wei Xie
    Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Nat Struct Biol 10:781-8. 2003
  6. ncbi An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli
    Jeffrey D Kittendorf
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Chuirch Street, Ann Arbor, MI 48109 1065, USA
    J Biol Chem 278:42369-76. 2003
  7. ncbi Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Med Chem 46:1133-43. 2003
  8. ncbi De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity
    Emmanuel A Meyer
    , , HCI, , Switzerland
    Chembiochem 3:250-3. 2002
  9. ncbi A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase
    U Grädler
    Institut fur Pharmazeutische Chemie, Marbacher Weg 6, Philipps Universitat Marburg, 35032, Germany
    J Mol Biol 306:455-67. 2001
  10. ncbi Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity
    U Grädler
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Germany
    FEBS Lett 454:142-6. 1999

Detail Information

Publications16

  1. ncbi Cloning and molecular characterization of the DNA ligase gene (lig) from Zymomonas mobilis
    K B Shark
    School of Biological Sciences, University of Nebraska, Lincoln 68588 0118
    FEMS Microbiol Lett 75:19-26. 1992
    ..coli enzyme. Two genes located upstream of lig were identified as tgt, encoding tRNA guanine transglycosylase and uvrB, encoding the beta subunit of excision endonuclease...
  2. ncbi Crystal structures of tRNA-guanine transglycosylase (TGT) in complex with novel and potent inhibitors unravel pronounced induced-fit adaptations and suggest dimer formation upon substrate binding
    Bernhard Stengl
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 370:492-511. 2007
    ..It is hypothesized that one unit of the dimer performs the catalytic reaction whereas the second is required to recognize and properly orient the bound tRNA for the catalytic reaction...
  3. ncbi Crystallographic study of inhibitors of tRNA-guanine transglycosylase suggests a new structure-based pharmacophore for virtual screening
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 338:55-75. 2004
    ..A virtual screening has been performed based on this pharmacophore hypothesis and several new inhibitors of micromolar binding affinity with new skeletons have been discovered...
  4. ncbi Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    Chembiochem 4:1066-77. 2003
    ..In consequence, full understanding of this plasticity can be exploited for drug design...
  5. ncbi Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate
    Wei Xie
    Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Nat Struct Biol 10:781-8. 2003
    Prokaryotic tRNA guanine transglycosylase (TGT) catalyzes replacement of guanine (G) by 7-aminomethyl-7-deazaguanine (PreQ1) at the wobble position of four specific tRNAs...
  6. ncbi An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli
    Jeffrey D Kittendorf
    Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Chuirch Street, Ann Arbor, MI 48109 1065, USA
    J Biol Chem 278:42369-76. 2003
    ..The results of these studies support two roles for aspartate 264 in catalysis by TGT, protonation of the leaving guanine and deprotonation of the incoming preQ1...
  7. ncbi Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis
    Ruth Brenk
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Med Chem 46:1133-43. 2003
    ..All nine tested inhibitors being representatives of these classes showed activity in the micromolar range, two of them even in the submicromolar range...
  8. ncbi De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity
    Emmanuel A Meyer
    , , HCI, , Switzerland
    Chembiochem 3:250-3. 2002
  9. ncbi A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase
    U Grädler
    Institut fur Pharmazeutische Chemie, Marbacher Weg 6, Philipps Universitat Marburg, 35032, Germany
    J Mol Biol 306:455-67. 2001
    ..The 1.95 A crystal structure of APH in complex with Z. mobilis TGT served as a starting point for further modification of this initial lead...
  10. ncbi Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity
    U Grädler
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Germany
    FEBS Lett 454:142-6. 1999
    ..The crystal structure of a TGT(S103A)/preQ1 complex combined with biochemical data presented in this paper suggest that Ser103 is essential for substrate orientation in the TGT reaction...
  11. ncbi Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase reveal aspartate 102 as the active site nucleophile
    C Romier
    European Molecular Biology Laboratory, Structural Biology Programme, Heidelberg, Germany
    Biochemistry 35:15734-9. 1996
    ..The mutants display only subtle changes to the wild-type protein, confirming that the observed biochemical results are due to the chemical substitutions rather than structural rearrangements...
  12. ncbi Purification, crystallization, and preliminary x-ray diffraction studies of tRNA-guanine transglycosylase from Zymomonas mobilis
    C Romier
    European Molecular Biology Laboratory, Structural Biology Programme, Heidelberg, Germany
    Proteins 24:516-9. 1996
    ..1 A, b = 65.1 A, c = 71.9 A, and beta = 97.5 degrees and contain one molecule per asymmetric unit. A complete diffraction data set from one native crystal has been obtained at 1.85 A resolution...
  13. ncbi Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies
    Naomi Tidten
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 374:764-76. 2007
    ..The way this is achieved, however, significantly differs from that predicted based on crystal structures of wild-type Tgt...
  14. ncbi Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange
    C Romier
    European Molecular Biology Laboratory, Structural Biology Programme, Heidelberg, Germany
    EMBO J 15:2850-7. 1996
    ..This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid...
  15. ncbi Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme
    K Reuter
    Institut fur Biochemie, Universitat Erlangen Nurnberg, Germany
    J Bacteriol 177:5284-8. 1995
    ..mobilis Tgt was found to be a monomer according to gel filtration. In this study, it was shown that the formation of homotrimers by the E. coli enzyme is readily reversible and is dependent on protein concentration...