tolC

Summary

Gene Symbol: tolC
Description: transport channel
Alias: ECK3026, JW5503, colE1-i, mtcB, mukA, refI, toc, weeA
Species:

Top Publications

  1. ncbi Kinetic control of TolC recruitment by multidrug efflux complexes
    Elena B Tikhonova
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA
    Proc Natl Acad Sci U S A 106:16416-21. 2009
  2. ncbi Metabolic shutdown in Escherichia coli cells lacking the outer membrane channel TolC
    Girija Dhamdhere
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA
    Mol Microbiol 77:743-54. 2010
  3. ncbi The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC
    Satoshi Nagakubo
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki shi, Japan
    J Bacteriol 184:4161-7. 2002
  4. ncbi Assembly and channel opening in a bacterial drug efflux machine
    Vassiliy N Bavro
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Mol Cell 30:114-21. 2008
  5. ncbi Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism
    Markus A Seeger
    Institute of Physiology and Zurich Centre for Integrative Human Physiology ZIHP, University of Zurich, Winterthurerstrasse 190, Zurich, Switzerland
    Science 313:1295-8. 2006
  6. ncbi The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli
    Natthawut Wiriyathanawudhiwong
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Appl Microbiol Biotechnol 81:903-13. 2009
  7. ncbi Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli
    Yoko Eguchi
    Department of Bioscience and Biotechnology, Graduate School of Agriculture of Kinki University, 3327 204, Nakamachi, Nara 631 8505, Japan
    Microbiology 149:2819-28. 2003
  8. ncbi Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export
    V Koronakis
    Department of Pathology, University of Cambridge, UK
    Nature 405:914-9. 2000
  9. ncbi EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB
    O Lomovskaya
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402, USA
    J Bacteriol 177:2328-34. 1995
  10. ncbi TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli
    Corinna Bleuel
    Institut fur Mikrobiologie, Martin Luther Universität Halle, Halle, Germany, Kurt Mothes Str 3, 06120 Halle, Germany
    J Bacteriol 187:6701-7. 2005

Research Grants

  1. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2005
  2. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2007
  3. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2009
  4. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 1990
  5. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 1980
  6. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications118 found, 100 shown here

  1. ncbi Kinetic control of TolC recruitment by multidrug efflux complexes
    Elena B Tikhonova
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA
    Proc Natl Acad Sci U S A 106:16416-21. 2009
    ..to the membrane fusion protein (MFP) family; and outer membrane channels exemplified by the Escherichia coli TolC. The three-component complexes span the entire two-membrane envelope of Gram-negative bacteria and expel toxic ..
  2. ncbi Metabolic shutdown in Escherichia coli cells lacking the outer membrane channel TolC
    Girija Dhamdhere
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA
    Mol Microbiol 77:743-54. 2010
    The outer membrane channel TolC is a key component of multidrug efflux and type I secretion transporters in Escherichia coli...
  3. ncbi The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC
    Satoshi Nagakubo
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki shi, Japan
    J Bacteriol 184:4161-7. 2002
    ..A BLAST search suggested that such a heteromultimer-type RND exporter constitutes a unique family among gram-negative organisms...
  4. ncbi Assembly and channel opening in a bacterial drug efflux machine
    Vassiliy N Bavro
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Mol Cell 30:114-21. 2008
    ..We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state...
  5. ncbi Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism
    Markus A Seeger
    Institute of Physiology and Zurich Centre for Integrative Human Physiology ZIHP, University of Zurich, Winterthurerstrasse 190, Zurich, Switzerland
    Science 313:1295-8. 2006
    The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump...
  6. ncbi The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli
    Natthawut Wiriyathanawudhiwong
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
    Appl Microbiol Biotechnol 81:903-13. 2009
    ..coli K-12 mutants (the Keio collection). Among the 3,985 nonessential gene mutants, tolC-disrupted cells showed hypersensitivity to L-cysteine relative to wild-type cells...
  7. ncbi Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli
    Yoko Eguchi
    Department of Bioscience and Biotechnology, Graduate School of Agriculture of Kinki University, 3327 204, Nakamachi, Nara 631 8505, Japan
    Microbiology 149:2819-28. 2003
    ..The observed MDR also depended on an outer-membrane channel, TolC. Microarray and S1 mapping assays indicated that, in the presence of this constitutive mutant EvgS, the level of ..
  8. ncbi Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export
    V Koronakis
    Department of Pathology, University of Cambridge, UK
    Nature 405:914-9. 2000
    ..by the reversible interaction of substrate-specific inner-membrane proteins with an outer-membrane protein of the TolC family, thus bypassing the intervening periplasm. Here we report the 2...
  9. ncbi EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB
    O Lomovskaya
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402, USA
    J Bacteriol 177:2328-34. 1995
    ..Some of the chemicals that induce the pump serve as its substrates, suggesting that their extrusion is the natural function of the pump...
  10. ncbi TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli
    Corinna Bleuel
    Institut fur Mikrobiologie, Martin Luther Universität Halle, Halle, Germany, Kurt Mothes Str 3, 06120 Halle, Germany
    J Bacteriol 187:6701-7. 2005
    ..and high-performance liquid chromatography analyses demonstrated that the outer membrane channel tunnel protein TolC but none of the respective seven resistance nodulation cell division (RND) proteins CusA, AcrB, AcrD, AcrF, MdtF (..
  11. ncbi Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Mol Microbiol 69:1450-5. 2008
    SUMMARY: The Escherichia coli tolC encodes a major outer membrane protein with multiple functions in export (e.g. diverse xenobiotics, haemolysin) and as an attachment site for phage and colicins...
  12. ncbi TolC-dependent exclusion of porphyrins in Escherichia coli
    Ryoko Tatsumi
    Department of Bioengineering, Tokyo Institute of Technology, 4259 J2 14 Nagatsuta, Midori ku, Yokohama 226 8503, Japan
    J Bacteriol 190:6228-33. 2008
    We found that Escherichia coli tolC mutants showed increased sensitivity to 5-aminolevulinic acid (ALA), a precursor of porphyrins...
  13. ncbi An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants
    Judah L Rosner
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 191:5283-92. 2009
    Efflux pumps function to rid bacteria of xenobiotics, including antibiotics, bile salts, and organic solvents. TolC, which forms an outer membrane channel, is an essential component of several efflux pumps in Escherichia coli...
  14. ncbi A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps
    Sune Lobedanz
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 104:4612-7. 2007
    ..A key event in pump assembly is the recruitment of an outer membrane-anchored TolC exit duct by the adaptor protein of a cognate inner membrane translocase, establishing a contiguous transenvelope ..
  15. ncbi Structure of the periplasmic component of a bacterial drug efflux pump
    Matthew K Higgins
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 101:9994-9. 2004
    ..consist of an inner membrane transporter such as the AcrB proton antiporter, an outer membrane exit duct of the TolC family, and a periplasmic protein known as the adaptor...
  16. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
    AcrB is a principal multidrug efflux transporter in Escherichia coli that cooperates with an outer-membrane channel, TolC, and a membrane-fusion protein, AcrA. Here we describe crystal structures of AcrB with and without substrates...
  17. ncbi Assembly of TolC, a structurally unique and multifunctional outer membrane protein of Escherichia coli K-12
    John Werner
    Molecular and Cellular Biology Graduate Program, Arizona State University, Tempe, Arizona 85287, USA
    J Bacteriol 185:6540-7. 2003
    b>TolC is a multifunctional outer membrane protein of Escherichia coli that folds into a novel alpha-beta-barrel conformation absent in the other model outer membrane proteins used in assembly studies...
  18. ncbi From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli
    Lina Wu
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou 510275, China
    J Proteome Res 8:1059-70. 2009
    ..Among the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  19. ncbi Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria
    C Andersen
    Department of Biotechnology, University of Wurzburg, 97074 Wurzburg, Germany
    Rev Physiol Biochem Pharmacol 147:122-65. 2003
    ..Prototypes of these export systems are the hemolysin secretion system and the AcrAB/TolC drug efflux pump of Escherichia coli, which both employ TolC as an outer membrane component...
  20. ncbi Transition to the open state of the TolC periplasmic tunnel entrance
    Christian Andersen
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 99:11103-8. 2002
    The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane complexes...
  21. ncbi A coordinated network of transporters with overlapping specificities provides a robust survival strategy
    Nir Tal
    Department of Biological Chemistry, Alexander A Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 106:9051-6. 2009
    ..in an organism such as Escherichia coli mutations in genes coding for transporters other than the major AcrAB-TolC multidrug efflux transporter have only a marginal effect on phenotype...
  22. ncbi Initial steps of colicin E1 import across the outer membrane of Escherichia coli
    Muriel Masi
    School of Life Sciences, Arizona State University, Tempe, AZ 85287 4501, USA
    J Bacteriol 189:2667-76. 2007
    ..ColE1 initially binds to the vitamin B(12) receptor BtuB and then translocates through the TolC channel-tunnel, presumably in a mostly unfolded state. Here, we studied the early events in the import of ColE1...
  23. ncbi Electrophysiological behavior of the TolC channel-tunnel in planar lipid bilayers
    C Andersen
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK
    J Membr Biol 185:83-92. 2002
    Escherichia coli TolC assembles into the unique channel-tunnel structure spanning the outer membrane and periplasmic space...
  24. ncbi Antibiotic-sensitive TolC mutants and their suppressors
    Anne Marie Augustus
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA
    J Bacteriol 186:1851-60. 2004
    The TolC protein of Escherichia coli, through its interaction with AcrA and AcrB, is thought to form a continuous protein channel that expels inhibitors from the cell. Consequently, tolC null mutations display a hypersensitive phenotype...
  25. ncbi Structure and function of TolC: the bacterial exit duct for proteins and drugs
    Vassilis Koronakis
    Department of Pathology, Cambridge University, Cambridge CB2 1QP, United Kingdom
    Annu Rev Biochem 73:467-89. 2004
    The bacterial TolC protein plays a common role in the expulsion of diverse molecules, which include protein toxins and antibacterial drugs, from the cell...
  26. ncbi YaeT (Omp85) affects the assembly of lipid-dependent and lipid-independent outer membrane proteins of Escherichia coli
    John Werner
    Molecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ 85287, USA
    Mol Microbiol 57:1450-9. 2005
    ..the two distinct groups of OMPs that follow either SurA- and lipopolysaccharide-dependent (OmpF/C) or -independent (TolC) folding pathways were affected, suggesting that YaeT may act as a general OMP assembly factor...
  27. ncbi Directed evolution of a bacterial efflux pump: adaptation of the E. coli TolC exit duct to the Pseudomonas MexAB translocase
    Evert Bokma
    Cambridge University Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    FEBS Lett 580:5339-43. 2006
    Bacterial multidrug efflux pumps operate by periplasmic recruitment and opening of TolC family outer membrane exit ducts by cognate inner membrane translocases...
  28. ncbi Studies on the region involved in the transport activity of Escherichia coli TolC by chimeric protein analysis
    Hiroyasu Yamanaka
    Laboratory of Molecular Microbiological Science, Faculty of Pharmaceutical Sciences, Hiroshima International University, Hiro Koshingai, Kure, Hiroshima 737 0112, Japan
    Microb Pathog 42:184-92. 2007
    Gram-negative bacteria possess the outer membrane protein TolC which acts as an exit duct across the outer membrane. However, the region involved in the transport activity of TolC has remained unclear...
  29. ncbi Importance of the adaptor (membrane fusion) protein hairpin domain for the functionality of multidrug efflux pumps
    Johannes F Stegmeier
    Universitat Wurzburg, Lehrstuhl fur Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
    Biochemistry 45:10303-12. 2006
    ..we have modeled the interaction of the hairpin domain and the channel tunnel on a molecular level for AcrA and TolC as well as MexA and OprM, respectively...
  30. ncbi Direct interaction of multidrug efflux transporter AcrB and outer membrane channel TolC detected via site-directed disulfide cross-linking
    Norihisa Tamura
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Biochemistry 44:11115-21. 2005
    The AcrAB-TolC system exports a wide variety of drugs and toxic compounds, and confers intrinsic drug tolerance on Escherichia coli. The crystal structures suggested that AcrB and TolC directly dock with each other...
  31. ncbi Identification and network of outer membrane proteins regulating streptomysin resistance in Escherichia coli
    Hui Li
    Center for Proteomics, State Key Laboratory of Bio Control, School of Life Sciences, Sun Yat Sen University, Guangzhou, PRC
    J Proteome Res 7:4040-9. 2008
    ..b>TolC, OmpT and LamB were found to be up-regulated, and FadL, OmpW and a location-unknown protein Dps were down-regulated ..
  32. ncbi Amino-acid residues involved in the expression of the activity of Escherichia coli TolC
    Hiroyasu Yamanaka
    Laboratory of Biochemistry, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Japan
    Microbiol Immunol 48:713-22. 2004
    The Escherichia coli TolC, composed of 471 amino-acid residues, functions as a channel tunnel in the transport of various molecules across the outer membrane...
  33. ncbi Genetic evidence for functional interactions between TolC and AcrA proteins of a major antibiotic efflux pump of Escherichia coli
    Henri Gerken
    Microbiology Graduate Program, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA
    Mol Microbiol 54:620-31. 2004
    Genetic data have suggested that TolC, AcrA and AcrB constitute a major antibiotic efflux system in Escherichia coli...
  34. ncbi Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system
    Thierry Touze
    Cambridge University Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
    Mol Microbiol 53:697-706. 2004
    The major Escherichia coli multidrug efflux pump AcrAB-TolC expels a wide range of antibacterial agents...
  35. ncbi Carboxy-terminal region involved in activity of Escherichia coli TolC
    H Yamanaka
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro, Tokushima 770 8514, Japan
    J Bacteriol 183:6961-4. 2001
    The Escherichia coli TolC acts as a channel tunnel in the transport of various molecules across the outer membrane...
  36. ncbi Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A
    J K Davies
    J Bacteriol 123:102-17. 1975
    ..We have confirmed the position of previously isolated mutations on the Escherichia coli K-12 genetic map, and located approximately the loci conferring colicin resistance in some of the newly isolated mutants...
  37. ncbi Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12
    J Hackett
    Nucleic Acids Res 11:6487-95. 1983
    We present the nucleotide sequence of the tolC gene of Escherichia coli K12, and the amino acid sequence of the TolC protein (an outer membrane protein) as deduced from it...
  38. ncbi Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli
    J A Fralick
    Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79403, USA
    J Bacteriol 178:5803-5. 1996
    A study examining the influence of TolC on AcrA, AcrR, and MarR1 mutants indicates that functional TolC is required for the operation of the AcrAB efflux system and for the expression of the Mar phenotype...
  39. ncbi Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals
    V Koronakis
    Department of Pathology, Cambridge University, UK
    Mol Microbiol 23:617-26. 1997
    b>TolC is an outer membrane protein required for the export of virulence proteins and toxic compounds without a periplasmic intermediate...
  40. ncbi Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12
    R Aono
    Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
    J Bacteriol 180:938-44. 1998
    Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS...
  41. ncbi Cross-linked complex between oligomeric periplasmic lipoprotein AcrA and the inner-membrane-associated multidrug efflux pump AcrB from Escherichia coli
    H I Zgurskaya
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3206, USA
    J Bacteriol 182:4264-7. 2000
    ..multiple antimicrobial agents are produced through expression of the three-component multidrug efflux system AcrAB-TolC. AcrB is a proton-motive-force-dependent transporter located in the inner membrane, and AcrA and TolC are accessory ..
  42. ncbi The TolC protein of Escherichia coli K12 is synthesised in a precursor form
    J Hackett
    FEBS Lett 156:307-10. 1983
    We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate...
  43. ncbi TolC, an Escherichia coli outer membrane protein required for hemolysin secretion
    C Wandersman
    Unité de Génétique Moléculaire, URA 1149 du Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France
    Proc Natl Acad Sci U S A 87:4776-80. 1990
    ..B. (1986) Curr. Top. Microbiol. Immunol. 125, 159-181]. We show here that an E. coli outer membrane protein, the TolC protein, encoded by a gene not located in the hly cluster, is specifically required for hemolysin secretion...
  44. ncbi A model of a transmembrane drug-efflux pump from Gram-negative bacteria
    Juan Fernandez-Recio
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    FEBS Lett 578:5-9. 2004
    ..A representative pump from Escherichia coli is an assembly of the trimeric outer-membrane protein TolC, which is an allosteric channel, the trimeric inner-membrane proton-antiporter AcrB, and the periplasmic protein, ..
  45. ncbi Isolation and characterization of VceC gain-of-function mutants that can function with the AcrAB multiple-drug-resistant efflux pump of Escherichia coli
    Govindsamy Vediyappan
    Department of Microbiology and Immunology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Bacteriol 188:3757-62. 2006
    ..b>TolC is the outer membrane component of the resistance-nodulation-division AcrAB-TolC efflux pump of Escherichia coli...
  46. ncbi Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin
    H Vakharia
    Department of Microbiology, Arizona State University, Tempe, AZ 85287, USA
    J Bacteriol 183:6908-16. 2001
    This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin...
  47. ncbi Determination of selectivity and efficacy of fatty acid synthesis inhibitors
    Srinivas Kodali
    Department of Human and Animal Infectious Disease, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 280:1669-77. 2005
    ..2 to 0.4 microg/ml. Furthermore, the effectiveness, selectivity, and the in vitro and in vivo correlations of BABX as well as other fatty acid inhibitors were elucidated, which will aid in future drug discovery...
  48. ncbi Use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport
    A Azizan
    Department of Biochemistry, College of Medicine, University of Tennessee, Memphis 38163
    J Bacteriol 176:6653-62. 1994
    ..at reduced rates on minimal agar plates containing the long-chain fatty acid oleate (C18:1), (i) fadL-phoA, (ii) tolC-phoA, and (iii) tsp-phoA, fadL-phoA and tolC-phoA fusions were unable to grow on oleate as the sole carbon and ..
  49. ncbi Isolation and characterization of an Escherichia coli mutant tolerant to colicins Ia and Ib
    J Cardelli
    J Bacteriol 119:379-85. 1974
    ..The tolI mutant shows a reduced sensitivity to sodium azide and phenethylalcohol. It is suggested that tolI is deficient in some aspect of aerobic metabolism which must be operative for colicin I sensitivity...
  50. ncbi Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters
    K Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    J Bacteriol 183:1455-8. 2001
    ..Since overexpression of the emrKY operon, which is controlled by evgAS, could account only for deoxycholate resistance, the evgAS locus apparently controls expression of at least one other multidrug efflux operon...
  51. ncbi Fitting periplasmic membrane fusion proteins to inner membrane transporters: mutations that enable Escherichia coli AcrA to function with Pseudomonas aeruginosa MexB
    Ganesh Krishnamoorthy
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Room 208, Norman, OK 73019, USA
    J Bacteriol 190:691-8. 2008
    AcrAB-TolC from Escherichia coli is a multidrug efflux complex capable of transenvelope transport...
  52. ncbi Distribution and evolution of short tandem repeats in closely related bacterial genomes
    Edit Kassai-Jáger
    Department of Genetics, Eotvos Lorand University, Pazmany Peter setany 1 C, H 1117 Budapest, Hungary
    Gene 410:18-25. 2008
    ..Our results suggest that microsatellites, although considerably less abundant than in eukaryotic genomes, may nevertheless play an important role in the evolution of prokaryotic genomes and several gene families...
  53. ncbi An aspartate ring at the TolC tunnel entrance determines ion selectivity and presents a target for blocking by large cations
    Christian Andersen
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK
    Mol Microbiol 44:1131-9. 2002
    The TolC protein of Escherichia coli comprises an outer membrane beta-barrel channel and a contiguous alpha-helical tunnel spanning the periplasm, providing an exit duct for protein export and multidrug efflux...
  54. ncbi The C-terminal domain of AcrA is essential for the assembly and function of the multidrug efflux pump AcrAB-TolC
    Qiang Ge
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
    J Bacteriol 191:4365-71. 2009
    ..role of the C-terminal domain of Escherichia coli AcrA, a periplasmic component of the multidrug efflux pump AcrAB-TolC. Using trypsin proteolysis, we identified the proteolytically labile sites in the C-terminal domain (amino acid ..
  55. ncbi YojI of Escherichia coli functions as a microcin J25 efflux pump
    Monica A Delgado
    Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad Nacional de Tucumán, San Miguel de Tucuman, Argentina
    J Bacteriol 187:3465-70. 2005
    ..The outer membrane protein TolC in addition to YojI is required for export of microcin J25 out of the cell...
  56. ncbi Substrate competition studies using whole-cell accumulation assays with the major tripartite multidrug efflux pumps of Escherichia coli
    Christopher A Elkins
    Division of Microbiology, National Center for Toxicological Research, 3900 NCTR Drive, Jefferson, AR 72079 9502, USA
    Antimicrob Agents Chemother 51:923-9. 2007
    AcrAB-TolC is the major, constitutively expressed tripartite multidrug efflux system in Escherichia coli that recognizes various structurally unrelated molecules, including many antibiotics, dyes, and steroids...
  57. ncbi The AraC-family regulator GadX enhances multidrug resistance in Escherichia coli by activating expression of mdtEF multidrug efflux genes
    Kunihiko Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka, Japan
    J Infect Chemother 14:23-9. 2008
    ..caused by gadX overexpression was completely suppressed by deleting the multifunctional outer membrane channel gene tolC. TolC interacts with different drug efflux pumps...
  58. ncbi Bypassing the periplasm: reconstitution of the AcrAB multidrug efflux pump of Escherichia coli
    H I Zgurskaya
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3206, USA
    Proc Natl Acad Sci U S A 96:7190-5. 1999
    ..AcrA brings two membranes together, and under certain conditions may even cause the fusion of at least the outer leaflets of the membranes, contributing to the ability of the AcrAB-TolC system to pump drugs out directly into the medium.
  59. ncbi The multidrug resistance efflux complex, EmrAB from Escherichia coli forms a dimer in vitro
    Mikio Tanabe
    Division of Molecular Biosciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Biochem Biophys Res Commun 380:338-42. 2009
    ..Previous work has indicated that EmrAB-TolC from Escherichia coli is such a tripartite system, comprised of EmrB an MFS transporter, EmrA, a membrane fusion ..
  60. ncbi Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli
    Dan feng Zhang
    Center for Proteomics, School of Life Sciences, Xiamen University, Xiamen, China
    Int J Antimicrob Agents 32:315-9. 2008
    ..In this study, upregulation of FimD, Tsx, OmpW, OmpC and TolC and downregulation of LamB in response to tetracycline were detected and confirmed by two-dimensional gel ..
  61. ncbi Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli
    Satoshi Yamada
    Department of Bioscience, Fukui Prefectural University, Fukui, Japan
    Appl Environ Microbiol 72:4735-42. 2006
    ..These results indicate that the multidrug transporter Bcr in the major facilitator family is involved in L-cysteine export and overproduction in genetically engineered E. coli cells...
  62. ncbi AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex
    Elena B Tikhonova
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 73019, USA
    J Biol Chem 279:32116-24. 2004
    ..interactions between the inner and outer membrane components of the tri-partite multidrug efflux pump AcrAB-TolC from Escherichia coli...
  63. ncbi Multidrug resistance pump AcrAB-TolC is required for high-level, Tet(A)-mediated tetracycline resistance in Escherichia coli
    Ricardo E de Cristóbal
    Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad Nacional de Tucumán, Tucuman, Argentina
    J Antimicrob Chemother 58:31-6. 2006
    Starting from the observation that Escherichia coli tolC mutations severely reduced the high-level resistance to tetracycline afforded by Tn10- and plasmid-encoded Tet(A) pumps, we studied the mechanism of this susceptibility.
  64. ncbi Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10
    R Morona
    Mol Gen Genet 184:430-3. 1981
    We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10...
  65. ncbi Role for tandem duplication and lon protease in AcrAB-TolC- dependent multiple antibiotic resistance (Mar) in an Escherichia coli mutant without mutations in marRAB or acrRAB
    Herve Nicoloff
    Center for Adaptation Genetics and Drug Resistance, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
    J Bacteriol 188:4413-23. 2006
    ..of the basal quantities of MarA produced, which in turn increased the amount of multidrug effux pump AcrAB-TolC. However, in a mutant carrying only a lon mutation, the overproduced pump mediated little, if any, increased ..
  66. ncbi The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT
    W C Nelson
    Department of Biology, University of North Carolina at Chapel Hill 27599, USA
    J Biol Chem 270:28374-80. 1995
    ..These data, coupled with data presented in the accompanying report, suggest that TraYp and IHF bind independent of one another, forming a nucleo-protein complex with oriT that can be recognized and nicked by TraIp...
  67. ncbi The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins
    R Morona
    J Bacteriol 150:1016-23. 1982
    b>tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein...
  68. ncbi Penetration of colicin M into cells of Escherichia coli
    V Braun
    J Bacteriol 142:162-8. 1980
    ..These data suggest that the function controlled by the tonB gene is required for the translocation of colicin M from its initial binding site at the tonA-coded receptor protein to the target...
  69. ncbi Characterization of Escherichia coli mutants tolerant to bacteriocin JF246: two new classes of tolerant mutants
    J Foulds
    J Bacteriol 116:885-92. 1973
    ..The mutation in class F and class G mutants is located between 21 to 23 min on the E. coli chromosome. We propose to designate the loci of these mutations as tolF and tolG, respectively...
  70. ncbi Physical map of the tolC-htrP region of the Escherichia coli chromosome
    T P Yang
    Department of Microbiology and Immunology, Northeastern Ohio Universities College of Medicine, Rootstown 44272
    J Bacteriol 174:1700-1. 1992
  71. ncbi Transitions between closed and open conformations of TolC: the effects of ions in simulations
    Robert Schulz
    Jacobs University Bremen, Bremen, Germany
    Biophys J 96:3116-25. 2009
    ..Upon formation of an efflux pump, the aperture of its outer membrane protein TolC opens and thereby enables the extrusion of substrate molecules...
  72. ncbi The assembled structure of a complete tripartite bacterial multidrug efflux pump
    Martyn F Symmons
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 106:7173-8. 2009
    ..a periplasmic adaptor protein connects a substrate-binding inner membrane transporter to an outer membrane-anchored TolC-type exit duct...
  73. ncbi Kinetic behavior of the major multidrug efflux pump AcrB of Escherichia coli
    Keiji Nagano
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 106:5854-8. 2009
    ..Here we use intact cells of Escherichia coli containing the intact multiprotein complex AcrB-AcrA-TolC, and measure the kinetic constants for various cephalosporins, by assessing the periplasmic concentration of the ..
  74. ncbi The leucine-responsive regulatory protein, Lrp, modulates microcin J25 intrinsic resistance in Escherichia coli by regulating expression of the YojI microcin exporter
    Sergio B Socias
    Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad Nacional de Tucumán, Chacabuco 461, 4000 San Miguel de Tucuman, Tucuman, Argentina
    J Bacteriol 191:1343-8. 2009
    ..Exogenous leucine antagonizes the effect of Lrp, leading to a diminished expression of the pump and an increased susceptibility to microcin J25...
  75. ncbi Need for TolC, an Escherichia coli outer membrane protein, in the secretion of heat-stable enterotoxin I across the outer membrane
    H Yamanaka
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Yamashiro, Tokushima, 770 8514, Japan
    Microb Pathog 25:111-20. 1998
    ..In this study, we examined the effects of mutation of the tolC gene which encodes an E...
  76. ncbi Changes in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable state
    Alicia Muela
    Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco, Bilbao, Spain
    FEMS Microbiol Ecol 64:28-36. 2008
    ..However, proteins exclusive to the VBNC state were not detected...
  77. ncbi CRP regulator modulates multidrug resistance of Escherichia coli by repressing the mdtEF multidrug efflux genes
    Kunihiko Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
    J Antibiot (Tokyo) 61:120-7. 2008
    ..caused by crp deletion was completely suppressed by deletion of the multifunctional outer membrane channel gene tolC. TolC interacts with different drug efflux pumps. Among the twenty drug efflux pumps in E...
  78. ncbi Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses
    Erlend Bore
    MATFORSK, Norwegian Food Research Institute, Osloveien 1, N 1430 As, Norway
    Microbiology 153:935-46. 2007
    ..The results revealed that BC treatment might result in superoxide stress in E. coli...
  79. ncbi Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting
    Ranjana Pathania
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Nat Chem Biol 5:849-56. 2009
    ..We show that MAC13243 inhibits the function of the LolA protein and represents a new chemical probe of lipoprotein targeting in bacteria with promise as an antibacterial lead with Gram-negative selectivity...
  80. ncbi High hydrostatic pressure treatment impairs AcrAB-TolC pump resulting in differential loss of deoxycholate tolerance in Escherichia coli
    Taketo Kawarai
    Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan
    J Biosci Bioeng 100:613-6. 2005
    ..The AcrAB-TolC multi-drug resistance pump driven by proton motive force has been argued to be responsible for the tolerance to ..
  81. ncbi Quorum sensing and multidrug transporters in Escherichia coli
    Shirley Yang
    Department of Molecular Virology and Microbiology and Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:2386-91. 2006
    ..In Escherichia coli, there are at least three multidrug transporters (AcrAB/TolC, MdfA, and NorE) that exude fluoroquinolones...
  82. ncbi Multidrug efflux pumps of gram-negative bacteria
    H Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley 94720-3206, USA
    J Bacteriol 178:5853-9. 1996
  83. ncbi Escherichia coli outer membrane protein TolC is involved in production of the peptide antibiotic microcin J25
    M A Delgado
    Departamento de Bioquimica de la Nutricion, INSIBIO Chacabuco 461, 4000 San Miguel de Tucuman, Argentina
    J Bacteriol 181:1968-70. 1999
    A Tn5 insertion in tolC eliminated microcin J25 production. The mutation had little effect on the expression of the microcin structural gene and presumably acted by blocking microcin secretion...
  84. ncbi The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate
    Natalya Baranova
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3206, USA
    J Bacteriol 184:4168-76. 2002
    ..We accordingly propose to rename yegMNOB as mdtABCD (mdt for multidrug transporter). Finally, the expression of two other genes, yicO and ygcL, was shown to be regulated by BaeR, but it is not known if they play any roles in resistance...
  85. ncbi Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug efflux protein TolC
    Matthew K Higgins
    Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK
    J Mol Biol 342:697-702. 2004
    The trimeric TolC protein of Escherichia coli comprises an outer membrane beta-barrel and a contiguous alpha-helical barrel projecting across the periplasm. This provides a single 140 A long pore for multidrug efflux and protein export...
  86. ncbi Toxic waste disposal in Escherichia coli
    Robert B Helling
    Department of Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Bacteriol 184:3699-703. 2002
    ..Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role...
  87. ncbi Aminoglycosides are captured from both periplasm and cytoplasm by the AcrD multidrug efflux transporter of Escherichia coli
    Julio Ramos Aires
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    J Bacteriol 187:1923-9. 2005
    ..This result suggested that AcrD can also capture aminoglycosides from the periplasm to extrude them into the medium in intact cells, acting as a "periplasmic vacuum cleaner."..
  88. ncbi Type 1 protein secretion in bacteria, the ABC-transporter dependent pathway (review)
    I Barry Holland
    Institut de Genetique et Microbiologie, CNRS UMR 8621, Universite Paris Sud, Orsay Cedex, France
    Mol Membr Biol 22:29-39. 2005
    ..HlyB, (a half transporter) triggering, via a conformational change in HlyD, recruitment of the third component, TolC, into the transenvelope complex...
  89. ncbi Engraftment of tonsillar mononuclear cells in human skin/SCID mouse chimera--validation of a novel xenogeneic transplantation model for autoimmune diseases
    N Yamanaka
    Department of Otolaryngology, Wakayama Medical University, Wakayama, Japan
    Microbiol Immunol 45:507-14. 2001
    ..In this review, we addressed the existing deficiencies in our understanding of the relationship between tonsils and PPP by using emerging transplantation technology involving SCID mice...
  90. ncbi Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element
    K Kobayashi
    Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori ku, Yokohama 226 8501, Japan
    J Bacteriol 183:2646-53. 2001
    The AcrAB-TolC efflux pump plays an intrinsic role in resistance to hydrophobic solvents in Escherichia coli. E. coli OST5500 is hypersensitive to solvents due to inactivation of the acrB gene by insertion of IS30...
  91. ncbi Topology analysis of the colicin V export protein CvaA in Escherichia coli
    R C Skvirsky
    Department of Biology, University of Massachusetts Boston 02125, USA
    J Bacteriol 177:6153-9. 1995
    ..At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway...
  92. ncbi Role of multiple efflux pumps in Escherichia coli in indole expulsion
    K Kawamura-Sato
    Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi, Japan
    FEMS Microbiol Lett 179:345-52. 1999
    ..This mutant was more susceptible to the growth-inhibitory effect of indole than the parent. These results indicate that the AcrEF system plays a significant role in indole efflux...
  93. ncbi Translocases: a bacterial tunnel for drugs and proteins
    K Lewis
    Biotechnology Center, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA
    Curr Biol 10:R678-81. 2000
    ..The TolC protein links the translocases to the external environment...
  94. ncbi Locking TolC entrance helices to prevent protein translocation by the bacterial type I export apparatus
    Jeyanthy Eswaran
    Department of Pathology, Cambridge University, Tennis Court Road, UK
    J Mol Biol 327:309-15. 2003
    The periplasmic entrance of the TolC channel tunnel is sealed by close-packing of inner and outer coiled-coils, and it has been proposed that opening of the entrance is achieved by an iris-like realignment of the inner coiled-coils...
  95. ncbi Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells
    S Hiraga
    Department of Molecular Genetics, Kumamoto University Medical School, Japan
    J Bacteriol 171:1496-505. 1989
    ..Cloning of the mukA gene indicates that the mukA1 mutation is recessive and that the mukA gene is identical to the tolC gene coding for an outer membrane protein.
  96. ncbi Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12
    R Misra
    Department of Microbiology, University of Sydney, New South Wales, Australia
    J Bacteriol 169:4722-30. 1987
    Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12...
  97. ncbi Defining the role of the Escherichia coli chaperone SecB using comparative proteomics
    Louise Baars
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University SE-106 91 Stockholm, Sweden
    J Biol Chem 281:10024-34. 2006
    ..dependence of 12 secretory proteins affected by the secB null mutation (DegP, FhuA, FkpA, OmpT, OmpX, OppA, TolB, TolC, YbgF, YcgK, YgiW, and YncE) was confirmed by "classical" pulse-labeling experiments...
  98. ncbi Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes
    M C Sulavik
    Genome Therapeutics Corporation, Waltham, Massachusetts, USA
    Antimicrob Agents Chemother 45:1126-36. 2001
    ..Deletions in acrAB or tolC resulted in increased susceptibilities to the majority of compounds tested...
  99. ncbi Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli
    J R Klein
    Abteilung Mikrobiologie, Universitat Kaiserslautern, FRG
    Mol Gen Genet 230:230-40. 1991
    ..Overproduction of EnvC protein appears to be lethal to Escherichia coli. The envD gene, however, could be cloned and expressed at high levels under control of the tac promoter without deleterious effects on the host...
  100. ncbi Entry into and release of solvents by Escherichia coli in an organic-aqueous two-liquid-phase system and substrate specificity of the AcrAB-TolC solvent-extruding pump
    N Tsukagoshi
    Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori ku, Yokohama 226 8501, Japan
    J Bacteriol 182:4803-10. 2000
    ..The AcrAB-TolC efflux pump system is involved in maintaining intrinsic solvent resistance...

Research Grants23

  1. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2005
    ....
  2. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2007
    ..continue our studies of the structure and function of the major multidrug efflux pump of Escherichia coli, AcrAB-TolC, to understand why this transporter can handle such a wide range of compounds...
  3. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2009
    ..continue our studies of the structure and function of the major multidrug efflux pump of Escherichia coli, AcrAB-TolC, to understand why this transporter can handle such a wide range of compounds...
  4. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 1990
    ..This study has implications in the study of cellular differentiation and of the process of development of tumors, as the disappearance of gap junction channel is one of the earliest events in the development of solid tumor...
  5. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 1980
    ..The "barrier" function of the membrane is also under study. ..
  6. BIOCHEMISTRY OF BACTERIAL CELL MEMBRANES
    Hiroshi Nikaido; Fiscal Year: 2010
    ..continue our studies of the structure and function of the major multidrug efflux pump of Escherichia coli, AcrAB-TolC, to understand why this transporter can handle such a wide range of compounds...