TA_RS03155

Summary

Gene Symbol: TA_RS03155
Description: proteasome subunit beta
Alias: Ta0612
Species: Thermoplasma acidophilum DSM 1728

Top Publications

  1. L we J, Stock D, Jap B, Zwickl P, Baumeister W, Huber R. Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution. Science. 1995;268:533-9 pubmed
    ..The binding of a peptide aldehyde inhibitor marks the active site in the central cavity at the amino termini of the beta subunits and suggests a novel proteolytic mechanism...
  2. Smith D, Kafri G, Cheng Y, Ng D, Walz T, Goldberg A. ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell. 2005;20:687-98 pubmed publisher
  3. Rabl J, Smith D, Yu Y, Chang S, Goldberg A, Cheng Y. Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases. Mol Cell. 2008;30:360-8 pubmed publisher
    ..This mechanism differs from that of PA26/28, which lacks the HbYX motif and does not cause alpha subunit rotation. These findings demonstrated how the ATPases' C termini function to facilitate substrate entry...
  4. Stadtmueller B, Ferrell K, Whitby F, Heroux A, Robinson H, Myszka D, et al. Structural models for interactions between the 20S proteasome and its PAN/19S activators. J Biol Chem. 2010;285:13-7 pubmed publisher
    ..In the case of the PAN and 19S activators, a penultimate tyrosine/phenylalanine residue contacts the proteasome Gly-19 carbonyl oxygen to stabilize the open conformation...
  5. Yu Y, Smith D, Kim H, Rodriguez V, Goldberg A, Cheng Y. Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions. EMBO J. 2010;29:692-702 pubmed publisher
    ..This structure together with related mutagenesis data suggest how in eukaryotes certain proteasomal ATPases bind to specific pockets in an asymmetrical manner to regulate gate opening...
  6. Zwickl P, Grziwa A, P hler G, Dahlmann B, Lottspeich F, Baumeister W. Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase. Biochemistry. 1992;31:964-72 pubmed
    ..On the basis of circumstantial evidence we suggest that the alpha-subunits have regulatory and targeting functions, while the beta-subunits carry the active sites...
  7. Seem ller E, Lupas A, Stock D, L we J, Huber R, Baumeister W. Proteasome from Thermoplasma acidophilum: a threonine protease. Science. 1995;268:579-82 pubmed
    ..The conservation pattern of this residue in eukaryotic sequences suggests that at least three of the seven eukaryotic beta-type subunit branches should be proteolytically inactive...
  8. Smith D, Chang S, Park S, Finley D, Cheng Y, Goldberg A. Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry. Mol Cell. 2007;27:731-44 pubmed publisher
    ..Thus, the C termini of the proteasomal ATPases function like a "key in a lock" to induce gate opening and allow substrate entry...
  9. Religa T, Sprangers R, Kay L. Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR. Science. 2010;328:98-102 pubmed publisher
    ..Interconversion between these conformers on a time scale of seconds leads to a dynamic regulation of 20S CP proteolysis activity...

More Information

Publications15

  1. Steinbacher S, Seckler R, Miller S, Steipe B, Huber R, Reinemer P. Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer. Science. 1994;265:383-6 pubmed
    ..The interdigitation of the polypeptide chains at the carboxyl termini is important to protrimer formation in the folding pathway and to thermostability of the mature protein...
  2. Kisselev A, Akopian T, Goldberg A. Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes. J Biol Chem. 1998;273:1982-9 pubmed
  3. F rster A, Masters E, Whitby F, Robinson H, Hill C. The 1.9 A structure of a proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions. Mol Cell. 2005;18:589-99 pubmed publisher
    ..Surprisingly, biochemical observations implicate an equivalent interaction for the unrelated ATP-dependent activators PAN and PA700...
  4. PĆ¼hler G, Weinkauf S, Bachmann L, Muller S, Engel A, Hegerl R, et al. Subunit stoichiometry and three-dimensional arrangement in proteasomes from Thermoplasma acidophilum. EMBO J. 1992;11:1607-16 pubmed
    ..In contrast to some previous reports we have not been able to find stoichiometric amounts of RNA associated with highly purified proteolytically active proteasome preparations. ..
  5. Seemuller E, Lupas A, Baumeister W. Autocatalytic processing of the 20S proteasome. Nature. 1996;382:468-71 pubmed publisher
    ..Although serine is an acceptable active-site nucleophile for proteolysis, and cysteine for processing, only threonine is fully functional in both. This explains why threonine is universally conserved in active proteasome subunits...
  6. Akopian T, Kisselev A, Goldberg A. Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum. J Biol Chem. 1997;272:1791-8 pubmed
    ..Thus, processivity is an inherent feature of the 20 S proteasome, not requiring all active sites or ATP hydrolysis...