Gene Symbol: TK_RS00010
Description: intein-containing DNA-directed DNA polymerase
Species: Thermococcus kodakarensis KOD1
Takagi M, Nishioka M, Kakihara H, Kitabayashi M, Inoue H, Kawakami B, et al
. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Appl Environ Microbiol. 1997;63:4504-10 pubmed
..These characteristics enabled the KOD DNA polymerase to perform a more accurate PCR in a shorter reaction time...
Hashimoto H, Nishioka M, Fujiwara S, Takagi M, Imanaka T, Inoue T, et al
. Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. J Mol Biol. 2001;306:469-77 pubmed publisher
..The stabilization of the melted DNA structure at the forked-point may be correlated with the high PCR performance of KOD DNA polymerase, which is due to low error rate, high elongation rate and processivity...
Nishioka M, Fujiwara S, Takagi M, Imanaka T. Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic Acids Res. 1998;26:4409-12 pubmed
..kodakaraensis KOD1 was used as substrate. Therefore, it is suggested that these endonucleases are switching endonucleases whose function lies in the rearrangement of chromosomal DNA...
Matsumura H, Takahashi H, Inoue T, Yamamoto T, Hashimoto H, Nishioka M, et al
. Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. Proteins. 2006;63:711-5 pubmed publisher