Gene Symbol: XT2
Description: UDP-xylosyltransferase 2
Alias: ARABIDOPSIS THALIANA UDP-XYLOSYLTRANSFERASE 2, ATXT2, AtXXT2, T10P11.20, UDP-xylosyltransferase 2, XXT2, XYG XYLOSYLTRANSFERASE 2, UDP-xylosyltransferase 2
Species: thale cress
Chou Y, Pogorelko G, Zabotina O. Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes. Plant Physiol. 2012;159:1355-66 pubmed publisher
..thaliana), the 1,4-?-glucan synthase, Cellulose Synthase-Like C4 (CSLC4), and three xylosyltransferases, XXT1, XXT2, and XXT5, act in the Golgi to form the xylosylated glucan backbone during xyloglucan biosynthesis...
Zabotina O, Avci U, Cavalier D, Pattathil S, Chou Y, Eberhard S, et al
. Mutations in multiple XXT genes of Arabidopsis reveal the complexity of xyloglucan biosynthesis. Plant Physiol. 2012;159:1367-84 pubmed publisher
..New double mutants (xxt1 xxt5 and xxt2 xxt5) and a triple mutant (xxt1 xxt2 xxt5) were generated, characterized, and compared with three single mutants ..
Cavalier D, Keegstra K. Two xyloglucan xylosyltransferases catalyze the addition of multiple xylosyl residues to cellohexaose. J Biol Chem. 2006;281:34197-207 pubmed
..Natl. Acad. Sci. U. S. A. 99, 7797-7802). We expressed both AtXT1 and a second closely related gene, now called AtXT2, in insect cells and demonstrated that both have XT activity for cellopentaose and cellohexaose acceptor ..
Cavalier D, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, Freshour G, et al
. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component. Plant Cell. 2008;20:1519-37 pubmed publisher
..To determine whether two Arabidopsis thaliana genes that encode xylosyltransferases, XXT1 and XXT2, are involved in xyloglucan biosynthesis in vivo and to determine how the plant cell wall is affected by the lack ..
Lund C, Bromley J, StenbÃ¦k A, Rasmussen R, Scheller H, Sakuragi Y. A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus. J Exp Bot. 2015;66:85-97 pubmed publisher
..Our results show that Rluc-PCA coupled with transient expression in N. benthamiana is a fast and versatile method suitable for analysis of PPIs between Golgi resident proteins in an easy and mid-throughput fashion in planta. ..
Chou Y, Pogorelko G, Young Z, Zabotina O. Protein-protein interactions among xyloglucan-synthesizing enzymes and formation of Golgi-localized multiprotein complexes. Plant Cell Physiol. 2015;56:255-67 pubmed publisher
..been identified, including the glucan synthase CSLC4 (cellulose synthase-like C4), three xylosyltransferases (XXT1, XXT2 and XXT5), two galactosyltransferases (MUR3 and XLT2) and the fucosyltransferase FUT1...
Kong Y, PeÃ±a M, Renna L, Avci U, Pattathil S, Tuomivaara S, et al
. Galactose-depleted xyloglucan is dysfunctional and leads to dwarfism in Arabidopsis. Plant Physiol. 2015;167:1296-306 pubmed publisher
..Our study assigns a role for galactosylation in normal xyloglucan function and demonstrates that altering xyloglucan side chain structure disturbs diverse cellular and physiological processes. ..
Vuttipongchaikij S, Brocklehurst D, Steele King C, Ashford D, Gomez L, McQueen Mason S. Arabidopsis GT34 family contains five xyloglucan ?-1,6-xylosyltransferases. New Phytol. 2012;195:585-95 pubmed publisher
The Arabidopsis genome includes seven family 34 glycosyltransferase (GT34) encoding genes. XXT1 and XXT2 have previously been shown to encode XyG ?-1,6-xylosyltransferases, while knockout mutants of a third, XXT5, exhibit decreased XyG ..
Culbertson A, Tietze A, Tietze D, Chou Y, Smith A, Young Z, et al
. A homology model of Xyloglucan Xylosyltransferase 2 reveals critical amino acids involved in substrate binding. Glycobiology. 2016;26:961-972 pubmed
..A homology model of the xyloglucan xylosyltransferase 2 (XXT2) catalytic domain was built on the basis of the crystal structure of A64Rp...