Gene Symbol: SIZ1
Description: DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain-containing protein
Alias: AT5G60420, ATSIZ1, MUF9.5, MUF9_5, DNA-binding protein with MIZ/SP-RING zinc finger, PHD-finger and SAP domain-containing protein
Species: thale cress

Top Publications

  1. Park B, Song J, Seo H. Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1. Nat Commun. 2011;2:400 pubmed publisher
    ..The nitrate reductases, NIA1 and NIA2, are sumoylated by AtSIZ1, which dramatically increases their activity. Both sumoylated and non-sumoylated NIA1 and NIA2 can form dimers...
  2. Jin J, Jin Y, Lee J, Miura K, Yoo C, Kim W, et al. The SUMO E3 ligase, AtSIZ1, regulates flowering by controlling a salicylic acid-mediated floral promotion pathway and through affects on FLC chromatin structure. Plant J. 2008;53:530-40 pubmed
    Loss-of-function siz1 mutations caused early flowering under short days. siz1 plants have elevated salicylic acid (SA) levels, which are restored to wild-type levels by expressing nahG, bacterial salicylate hydroxylase...
  3. Miura K, Ohta M. SIZ1, a small ubiquitin-related modifier ligase, controls cold signaling through regulation of salicylic acid accumulation. J Plant Physiol. 2010;167:555-60 pubmed publisher
    ..The SA-accumulating mutant siz1 exhibits sensitivity to chilling and freezing conditions and decreased expression of DREB1A/CBF3 and its regulon ..
  4. Garcia Dominguez M, March Diaz R, Reyes J. The PHD domain of plant PIAS proteins mediates sumoylation of bromodomain GTE proteins. J Biol Chem. 2008;283:21469-77 pubmed publisher
    ..We have studied the Arabidopsis SUMO ligase AtSIZ1, belonging to the PIAS family, and describe self-sumoylation and AtSIZ1-mediated sumoylation of the E2 enzyme ..
  5. Saracco S, Miller M, Kurepa J, Vierstra R. Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007;145:119-34 pubmed
    ..This increase involves SUMO1 and SUMO2 and is mainly driven by the SUMO protein ligase SIZ1, with most of the conjugates accumulating in the nucleus...
  6. Ling Y, Zhang C, Chen T, Hao H, Liu P, Bressan R, et al. Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis. PLoS ONE. 2012;7:e29470 pubmed publisher
    ..Previous reports found that the small ubiquitin-like modifier (SUMO) E3 ligase, SIZ1, participated in many processes depending on particular target substrates and suppression of salicylic acid (SA) ..
  7. Miura K, Jin J, Lee J, Yoo C, Stirm V, Miura T, et al. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. Plant Cell. 2007;19:1403-14 pubmed
    b>SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates...
  8. Miura K, Lee J, Jin J, Yoo C, Miura T, Hasegawa P. Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling. Proc Natl Acad Sci U S A. 2009;106:5418-23 pubmed publisher
    ..This paper presents evidence that the SUMO E3 ligase SIZ1 negatively regulates abscisic acid (ABA) signaling, which is dependent on the bZIP transcripton factor ABI5...
  9. Yoo C, Miura K, Jin J, Lee J, Park H, Salt D, et al. SIZ1 small ubiquitin-like modifier E3 ligase facilitates basal thermotolerance in Arabidopsis independent of salicylic acid. Plant Physiol. 2006;142:1548-58 pubmed
    ..b>SIZ1 is a SUMO E3 ligase that facilitates SUMO conjugation to substrate target proteins (sumoylation) in Arabidopsis (..

More Information


  1. Catala R, Ouyang J, Abreu I, Hu Y, Seo H, Zhang X, et al. The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses. Plant Cell. 2007;19:2952-66 pubmed
    ..SUMO E3 ligase functions are executed by SIZ1/SIZ2 and Mms21 in yeast, the PIAS family members RanBP2, and Pc2 in human...
  2. Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan A, Raghothama K, et al. The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci U S A. 2005;102:7760-5 pubmed
    ..Herein, evidence establishes that AtSIZ1 is a plant small ubiquitin-like modifier (SUMO) E3 ligase and is a focal controller of Pi starvation-dependent ..
  3. Miura K, Lee J, Miura T, Hasegawa P. SIZ1 controls cell growth and plant development in Arabidopsis through salicylic acid. Plant Cell Physiol. 2010;51:103-13 pubmed publisher
    ..Previously, we demonstrated that the siz1 mutant, which is impaired in SUMO E3 ligase, showed a dwarf-like shoot phenotype with accumulation of salicylic ..
  4. Miura K, Lee J, Gong Q, Ma S, Jin J, Yoo C, et al. SIZ1 regulation of phosphate starvation-induced root architecture remodeling involves the control of auxin accumulation. Plant Physiol. 2011;155:1000-12 pubmed publisher
    ..Previously, we reported that the Arabidopsis (Arabidopsis thaliana) SUMO E3 ligase SIZ1 regulates root architecture remodeling in response to Pi limitation; namely, the siz1 mutations cause the ..
  5. Chen C, Chen Y, Tang I, Liang H, Lai C, Chiou J, et al. Arabidopsis SUMO E3 ligase SIZ1 is involved in excess copper tolerance. Plant Physiol. 2011;156:2225-34 pubmed publisher
    ..Arabidopsis thaliana), several stress-responsive SUMO conjugations are mediated mainly by the SUMO E3 ligase SIZ1. In this study, we observed a phenotype of hypersensitivity to excess copper in the siz1-2 and siz1-3 mutants...
  6. Cheong M, Park H, Hong M, Lee J, Choi W, Jin J, et al. Specific domain structures control abscisic acid-, salicylic acid-, and stress-mediated SIZ1 phenotypes. Plant Physiol. 2009;151:1930-42 pubmed publisher
    b>SIZ1 (for yeast SAP and MIZ1) encodes the sole ortholog of mammalian PIAS (for protein inhibitor of activated STAT) and yeast SIZ SUMO (for small ubiquitin-related modifier) E3 ligases in Arabidopsis (Arabidopsis thaliana)...
  7. Lee J, Nam J, Park H, Na G, Miura K, Jin J, et al. Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. Plant J. 2007;49:79-90 pubmed
    ..Yet little is known about the function of sumoylation in plants. Here, we show that the SIZ1 gene, which encodes an Arabidopsis SUMO E3 ligase, regulates innate immunity...
  8. Ishida T, Yoshimura M, Miura K, Sugimoto K. MMS21/HPY2 and SIZ1, two Arabidopsis SUMO E3 ligases, have distinct functions in development. PLoS ONE. 2012;7:e46897 pubmed publisher
    ..reported in yeast and animals, but only two types of E3 ligases have been identified in Arabidopsis: SAP and Miz 1 (SIZ1) and Methyl Methanesulfonate-Sensitivity protein 21 (MMS21)/HIGH PLOIDY 2 (HPY2), hereafter referred to as HPY2...
  9. Miller M, Barrett Wilt G, Hua Z, Vierstra R. Proteomic analyses identify a diverse array of nuclear processes affected by small ubiquitin-like modifier conjugation in Arabidopsis. Proc Natl Acad Sci U S A. 2010;107:16512-7 pubmed publisher
    ..Taken together, we propose that SUMOylation represents a rapid and global mechanism for reversibly manipulating plant chromosomal functions, especially during environmental stress. ..
  10. Castro P, Verde N, Lourenço T, Magalhães A, Tavares R, Bejarano E, et al. SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signaling and Metabolism in Arabidopsis thaliana. Plant Cell Physiol. 2015;56:2297-311 pubmed publisher
    ..The Arabidopsis SUMO E3 ligase SAP and Miz 1 (SIZ1) is the major SUMO conjugation enhancer in response to stress, and is implicated in several aspects of plant ..
  11. Miura K, Okamoto H, Okuma E, Shiba H, Kamada H, Hasegawa P, et al. SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis. Plant J. 2013;73:91-104 pubmed publisher
    ..Here, we demonstrate that SIZ1-mediated endogenous salicylic acid (SA) accumulation plays an important role in stomatal closure and drought ..
  12. Ajjawi I, Coku A, Froehlich J, Yang Y, Osteryoung K, Benning C, et al. A J-like protein influences fatty acid composition of chloroplast lipids in Arabidopsis. PLoS ONE. 2011;6:e25368 pubmed publisher
    ..These results provide a starting point for future investigations of how mutations in CJD1 affect lipid composition. ..
  13. Cheong M, Park H, Bohnert H, Bressan R, Yun D. Structural and functional studies of SIZ1, a PIAS-type SUMO E3 ligase from Arabidopsis. Plant Signal Behav. 2010;5:567-9 pubmed publisher
    ..Arabidopsis SIZ1, a SUMO E3 ligase, is an ortholog of the mammalian PIAS (Protein Inhibitor of Activated STAT) and yeast SIZ (SAP/..
  14. Kim D, Han Y, Kim S, Song J, Seo H. Arabidopsis CMT3 activity is positively regulated by AtSIZ1-mediated sumoylation. Plant Sci. 2015;239:209-15 pubmed publisher
    ..the DNA methylation activity of Arabidopsis CHROMOMETHYLASE 3 (CMT3) is positively regulated by the E3 SUMO ligase AtSIZ1. The methylation level of the Arabidopsis genome, including transposons, was significantly lower in the siz1-2 ..
  15. Miura K, Sato A, Ohta M, Furukawa J. Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2. Planta. 2011;234:1191-9 pubmed publisher
    ..We report here that mutations in SIZ1 or PHO2, which cause more accumulation of phosphate compared with the wild type, enhance tolerance to salt stress...
  16. Zheng Y, Schumaker K, Guo Y. Sumoylation of transcription factor MYB30 by the small ubiquitin-like modifier E3 ligase SIZ1 mediates abscisic acid response in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2012;109:12822-7 pubmed publisher
    ..A K283R substitution in MYB30 blocks its SUMO E3 ligase SIZ1-mediated sumoylation in Arabidopsis protoplasts, indicating that MYB30 is sumoylated by SIZ1 and that K283 is the ..
  17. Son G, Park B, Song J, Seo H. FLC-mediated flowering repression is positively regulated by sumoylation. J Exp Bot. 2014;65:339-51 pubmed publisher
    ..and in vitro pull-down analyses showed that FLC interacts with the E3 small ubiquitin-like modifier (SUMO) ligase AtSIZ1, suggesting that AtSIZ1 is an E3 SUMO ligase for FLC...
  18. Elrouby N, Bonequi M, Porri A, Coupland G. Identification of Arabidopsis SUMO-interacting proteins that regulate chromatin activity and developmental transitions. Proc Natl Acad Sci U S A. 2013;110:19956-61 pubmed publisher
    ..It reduces protein levels of CYCLING DOF FACTOR 2, hence increasing transcript levels of CONSTANS and promoting flowering through the photoperiodic pathway. ..
  19. Kim S, Park B, Kim D, Yeu S, Song S, Song J, et al. E3 SUMO ligase AtSIZ1 positively regulates SLY1-mediated GA signalling and plant development. Biochem J. 2015;469:299-314 pubmed publisher
    ..In the present study, we show that the E3 SUMO (small ubiquitin-related modifier) ligase AtSIZ1 regulates gibberellic acid signalling in Arabidopsis species by sumoylating SLY1...
  20. Park B, Kim S, Song J, Seo H. Arabidopsis SIZ1 positively regulates alternative respiratory bypass pathways. BMB Rep. 2012;45:342-7 pubmed
    ..These results indicate that low nitrate reductase activity due to the AtSIZ1 mutation is correlated with an overall decrease in alternative respiration and with a low carbohydrate content to ..
  21. Lin X, Niu D, Hu Z, Kim D, Jin Y, Cai B, et al. An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity. PLoS Genet. 2016;12:e1006016 pubmed publisher
    ..Here we show that SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. Loss-of-function siz1 mutant seedlings exhibit a weak constitutive photomorphogenic phenotype...
  22. Chen C, Chen Y, Yeh K. Effect of Cu content on the activity of Cu/ZnSOD1 in the Arabidopsis SUMO E3 ligase siz1 mutant. Plant Signal Behav. 2011;6:1428-30 pubmed publisher
    ..we found copper (Cu) accumulated to a higher level in the aerial parts of soil-grown plants of the SUMO E3 ligase siz1 mutant than in those of the wild type...
  23. Crozet P, Margalha L, Butowt R, Fernandes N, Elias C, Orosa B, et al. SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J. 2016;85:120-133 pubmed publisher
    ..Here, we demonstrate that the SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the E3 Small Ubiquitin-like Modifier (SUMO) ligase responsible for this modification...
  24. Kim S, Kwak J, Song J, Seo H. The E3 SUMO ligase AtSIZ1 functions in seed germination in Arabidopsis. Physiol Plant. 2016;158:256-271 pubmed publisher
    ..Here, we show that the E3 SUMO ligase AtSIZ1 regulates seed dormancy and germination...