Gene Symbol: RDR2
Description: RNA-dependent RNA polymerase 2
Alias: AtRDR2, RNA-dependent RNA polymerase 2, SILENCING MOVEMENT DEFICIENT 1, SMD1, T22B4.110, T22B4_110, RNA-dependent RNA polymerase 2
Species: thale cress

Top Publications

  1. Xie Z, Johansen L, Gustafson A, Kasschau K, Lellis A, Zilberman D, et al. Genetic and functional diversification of small RNA pathways in plants. PLoS Biol. 2004;2:E104 pubmed
    ..One RDR protein (RDR2) was required for all endogenous siRNAs analyzed...
  2. Herr A, Jensen M, Dalmay T, Baulcombe D. RNA polymerase IV directs silencing of endogenous DNA. Science. 2005;308:118-20 pubmed
    ..The existence of this distinct silencing polymerase may explain the paradoxical involvement of an RNA silencing pathway in maintenance of transcriptional silencing. ..
  3. Jia Y, Lisch D, Ohtsu K, Scanlon M, Nettleton D, Schnable P. Loss of RNA-dependent RNA polymerase 2 (RDR2) function causes widespread and unexpected changes in the expression of transposons, genes, and 24-nt small RNAs. PLoS Genet. 2009;5:e1000737 pubmed publisher
    ..RNA-dependent RNA polymerase 2 (RDR2) is a component of the RNA-directed DNA methylation (RdDM) silencing pathway...
  4. Pontes O, Li C, Costa Nunes P, Haag J, Ream T, Vitins A, et al. The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center. Cell. 2006;126:79-92 pubmed
    ..a pathway involving two forms of nuclear RNA polymerase IV (Pol IVa and Pol IVb), RNA-DEPENDENT RNA POLYMERASE 2 (RDR2), DICER-LIKE 3 (DCL3), ARGONAUTE4 (AGO4), the chromatin remodeler DRD1, and the de novo cytosine methyltransferase ..
  5. Blevins T, Podicheti R, Mishra V, Marasco M, Wang J, Rusch D, et al. Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis. elife. 2015;4:e09591 pubmed publisher
    ..24 nt siRNA biogenesis requires nuclear RNA polymerase IV (Pol IV), RNA-dependent RNA polymerase 2 (RDR2) and DICER-like 3 (DCL3). However, siRNA precursors are mostly undefined...
  6. Law J, Vashisht A, Wohlschlegel J, Jacobsen S. SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. PLoS Genet. 2011;7:e1002195 pubmed publisher
    ..that several previously identified RdDM components co-purify with Pol-IV, namely RNA-dependent RNA polymerase 2 (RDR2), CLASSY1 (CLSY1), and RNA-directed DNA methylation 4 (RDM4), suggesting that the upstream siRNA generating portion ..
  7. Zhang H, Ma Z, Zeng L, Tanaka K, Zhang C, Ma J, et al. DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV. Proc Natl Acad Sci U S A. 2013;110:8290-5 pubmed publisher
    ..Our results also suggest the involvement of DTF1 in an important negative feedback mechanism for DNA methylation at some RdDM target loci. ..
  8. Li S, Vandivier L, Tu B, Gao L, Won S, Li S, et al. Detection of Pol IV/RDR2-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis. Genome Res. 2015;25:235-45 pubmed publisher
    ..to transcribe RdDM loci to produce primary transcripts that are converted to double-stranded RNAs (dsRNAs) by RDR2 to serve as siRNA precursors. Yet, no such siRNA precursor transcripts have ever been reported...
  9. Deng S, Xu J, Liu J, Kim S, Shi S, Chua N. JMJ24 binds to RDR2 and is required for the basal level transcription of silenced loci in Arabidopsis. Plant J. 2015;83:770-82 pubmed publisher
    ..We also provide evidence of a physical association between JMJ24 and RNA-dependent RNA polymerase 2 (RDR2), which represents an evolved property of the RNA silencing pathway.

More Information


  1. Dunoyer P, Himber C, Voinnet O. DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal. Nat Genet. 2005;37:1356-60 pubmed
    ..At least three SILENCING MOVEMENT DEFICIENT genes (SMD1, SMD2 and SMD3) are required for trafficking, the extent of which correlates with siRNA levels in the veins...
  2. Brosnan C, Mitter N, Christie M, Smith N, Waterhouse P, Carroll B. Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis. Proc Natl Acad Sci U S A. 2007;104:14741-6 pubmed
    ..involved in a chromatin silencing pathway, NRPD1a encoding RNA polymerase IVa, RNA-dependent RNA polymerase 2 (RDR2), and DICER-like 3 (DCL3), are required for reception of long-distance mRNA silencing in the shoot...
  3. Tantikanjana T, Rizvi N, Nasrallah M, Nasrallah J. A dual role for the S-locus receptor kinase in self-incompatibility and pistil development revealed by an Arabidopsis rdr6 mutation. Plant Cell. 2009;21:2642-54 pubmed publisher
  4. Ronemus M, Vaughn M, Martienssen R. MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis. Plant Cell. 2006;18:1559-74 pubmed
    ..miRNA target genes accumulated in wild-type plants but not in ago1, the RNA-dependent RNA polymerase mutants rdr2 and rdr6, or the Dicer-like mutants dcl1 and dcl3...
  5. Lopez A, Ramirez V, GarcĂ­a Andrade J, Flors V, Vera P. The RNA silencing enzyme RNA polymerase v is required for plant immunity. PLoS Genet. 2011;7:e1002434 pubmed publisher
    ..were extended to other mutants affected in different steps of the RdDM pathway, such as nrpd1, nrpe1, ago4, drd1, rdr2, and drm1drm2 mutants...
  6. Fan D, Dai Y, Wang X, Wang Z, He H, Yang H, et al. IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis. Nucleic Acids Res. 2012;40:8905-16 pubmed publisher
    ..RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE 3 (DCL3) are necessary factors in 24-nt small interfering RNA (siRNA) biogenesis, which is part of ..
  7. Haag J, Ream T, Marasco M, Nicora C, Norbeck A, Pasa Tolic L, et al. In vitro transcription activities of Pol IV, Pol V, and RDR2 reveal coupling of Pol IV and RDR2 for dsRNA synthesis in plant RNA silencing. Mol Cell. 2012;48:811-8 pubmed publisher
    ..the presumptive DNA-dependent RNA polymerases Pol IV and Pol V and the putative RNA-dependent RNA polymerase RDR2. Here we demonstrate their RNA polymerase activities in vitro...
  8. Xie M, Ren G, Costa Nunes P, Pontes O, Yu B. A subgroup of SGS3-like proteins act redundantly in RNA-directed DNA methylation. Nucleic Acids Res. 2012;40:4422-31 pubmed publisher
    ..Thus, our results demonstrate that this group of SGS3-like proteins is an important component of RdDM. This study further enhances our understanding of the SGS3 gene family and the RdDM pathway. ..