Gene Symbol: RBOHD
Description: respiratory burst oxidase homologue D
Alias: ATRBOHD, MCA23.25, MCA23_25, RESPIRATORY BURST OXIDASE, respiratory burst oxidase homologue D, respiratory burst oxidase homologue D
Species: thale cress

Top Publications

  1. Torres M, Dangl J, Jones J. Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc Natl Acad Sci U S A. 2002;99:517-22 pubmed
    ..The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues (rboh genes) have been proposed to play a role in ROI generation...
  2. Davletova S, Rizhsky L, Liang H, Shengqiang Z, Oliver D, Coutu J, et al. Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell. 2005;17:268-81 pubmed
    ..Using knockout plants for the NADPH oxidase D protein (knockout-RbohD), we demonstrate that RbohD might be required for ROS signal amplification during light stress...
  3. Sagi M, Fluhr R. Production of reactive oxygen species by plant NADPH oxidases. Plant Physiol. 2006;141:336-40 pubmed
  4. Ogasawara Y, Kaya H, Hiraoka G, Yumoto F, Kimura S, Kadota Y, et al. Synergistic activation of the Arabidopsis NADPH oxidase AtrbohD by Ca2+ and phosphorylation. J Biol Chem. 2008;283:8885-92 pubmed publisher
    Plant respiratory burst oxidase homolog (rboh) proteins, which are homologous to the mammalian 91-kDa glycoprotein subunit of the phagocyte oxidase (gp91(phox)) or NADPH oxidase 2 (NOX2), have been implicated in the production of ..
  5. Evans M, Choi W, Gilroy S, Morris R. A ROS-Assisted Calcium Wave Dependent on the AtRBOHD NADPH Oxidase and TPC1 Cation Channel Propagates the Systemic Response to Salt Stress. Plant Physiol. 2016;171:1771-84 pubmed publisher
    ..the NADPH oxidase inhibitor diphenyliodonium and analysis of Ca(2+) wave propagation in the Arabidopsis respiratory burst oxidase homolog D (AtrbohD) knockout background all led to reductions in Ca(2+) wave transmission speeds ..
  6. Morales J, Kadota Y, Zipfel C, Molina A, Torres M. The Arabidopsis NADPH oxidases RbohD and RbohF display differential expression patterns and contributions during plant immunity. J Exp Bot. 2016;67:1663-76 pubmed publisher
    Plant NADPH oxidases, also known as respiratory burst oxidase homologues (RBOHs), produce reactive oxygen species (ROS) that perform a wide range of functions...
  7. Torres M, Jones J, Dangl J. Pathogen-induced, NADPH oxidase-derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana. Nat Genet. 2005;37:1130-4 pubmed
    ..Mutation of Arabidopsis thaliana respiratory burst oxidase (Atrboh) genes eliminated pathogen-induced ROI production but had only a modest effect on the ..
  8. Peer M, Bach M, Mueller M, Waller F. Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves. FEBS Lett. 2011;585:3006-10 pubmed publisher
    ..ROS production was independent of cell death induction and required the NADPH oxidase Respiratory Burst Oxidase Homolog D (RBOHD)...
  9. Suzuki N, Miller G, Salazar C, Mondal H, Shulaev E, Cortes D, et al. Temporal-spatial interaction between reactive oxygen species and abscisic acid regulates rapid systemic acclimation in plants. Plant Cell. 2013;25:3553-69 pubmed publisher

More Information


  1. Wormit A, Butt S, Chairam I, McKenna J, Nunes Nesi A, Kjaer L, et al. Osmosensitive changes of carbohydrate metabolism in response to cellulose biosynthesis inhibition. Plant Physiol. 2012;159:105-17 pubmed publisher
    ..activity1 [mca1]) and osmoperception (cytokinin receptor1 [cre1]) or reactive oxygen species production (respiratory burst oxidase homolog DF [rbohDF])...
  2. Maruta T, Inoue T, Tamoi M, Yabuta Y, Yoshimura K, Ishikawa T, et al. Arabidopsis NADPH oxidases, AtrbohD and AtrbohF, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor. Plant Sci. 2011;180:655-60 pubmed publisher
    To clarify genetically the involvement of two Arabidopsis NADPH oxidases (AtrbohD and AtrbohF) in the jasmonic acid (JA) signaling pathway, we characterized single knockout mutants lacking either Atrboh...
  3. Siddique S, Matera C, Radakovic Z, Hasan M, Gutbrod P, Rozanska E, et al. Parasitic worms stimulate host NADPH oxidases to produce reactive oxygen species that limit plant cell death and promote infection. Sci Signal. 2014;7:ra33 pubmed publisher
    ..that infection of Arabidopsis thaliana by the parasitic nematode Heterodera schachtii activated the NADPH oxidases RbohD and RbohF to produce ROS, which was necessary to restrict infected plant cell death and promote nurse cell ..
  4. Gonzali S, Loreti E, Cardarelli F, Novi G, Parlanti S, Pucciariello C, et al. Universal stress protein HRU1 mediates ROS homeostasis under anoxia. Nat Plants. 2015;1:15151 pubmed publisher
    ..reveal that HRU1 interacts with proteins that induce ROS production, the GTPase ROP2 and the NADPH oxidase RbohD, pointing to the existence of a low-oxygen-specific mechanism for the modulation of ROS levels...
  5. Ben Rejeb K, Benzarti M, Debez A, Bailly C, Savouré A, Abdelly C. NADPH oxidase-dependent H2O2 production is required for salt-induced antioxidant defense in Arabidopsis thaliana. J Plant Physiol. 2015;174:5-15 pubmed publisher
    ..Double mutant atrbohd/f plants failed to induce the antioxidant response under the culture conditions...
  6. Miller G, Schlauch K, Tam R, Cortes D, Torres M, Shulaev V, et al. The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli. Sci Signal. 2009;2:ra45 pubmed publisher
    ..4 centimeters per minute and was dependent on the respiratory burst oxidase homolog D (RbohD) gene...
  7. Yun B, Feechan A, Yin M, Saidi N, Le Bihan T, Yu M, et al. S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature. 2011;478:264-8 pubmed publisher
    ..a negative feedback loop limiting the hypersensitive response, mediated by S-nitrosylation of the NADPH oxidase, AtRBOHD, at Cys 890, abolishing its ability to synthesize reactive oxygen intermediates...
  8. Torres M, Morales J, Sánchez Rodríguez C, Molina A, Dangl J. Functional interplay between Arabidopsis NADPH oxidases and heterotrimeric G protein. Mol Plant Microbe Interact. 2013;26:686-94 pubmed publisher
    ..We analyze the interaction between NADPH oxidases AtRbohD and AtRbohF and the Arabidopsis heterotrimeric G protein...
  9. Dubiella U, Seybold H, Durian G, Komander E, Lassig R, Witte C, et al. Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation. Proc Natl Acad Sci U S A. 2013;110:8744-9 pubmed publisher
    ..Using selected reaction monitoring MS, we identified the plant NADPH oxidase, respiratory burst oxidase homolog D (RBOHD), as an in vivo phosphorylation target of CPK5...
  10. Pogány M, von Rad U, Grün S, Dongo A, Pintye A, Simoneau P, et al. Dual roles of reactive oxygen species and NADPH oxidase RBOHD in an Arabidopsis-Alternaria pathosystem. Plant Physiol. 2009;151:1459-75 pubmed publisher
    ..Here, we present dual roles of RBOHD (for respiratory burst oxidase homolog D) in an Arabidopsis-Alternaria pathosystem, suggesting either initiation or prevention of cell ..
  11. Jiao Y, Sun L, Song Y, Wang L, Liu L, Zhang L, et al. AtrbohD and AtrbohF positively regulate abscisic acid-inhibited primary root growth by affecting Ca2+ signalling and auxin response of roots in Arabidopsis. J Exp Bot. 2013;64:4183-92 pubmed publisher
    Reactive oxygen species (ROS) originating from the NADPH oxidases AtrbohD and AtrbohF play an important role in abscisic acid (ABA)-inhibited primary root growth in Arabidopsis...
  12. Larkindale J, Hall J, Knight M, Vierling E. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 2005;138:882-97 pubmed
  13. Joo J, Wang S, Chen J, Jones A, Fedoroff N. Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone. Plant Cell. 2005;17:957-70 pubmed
    ..triggered by extracellular ROS signals produced by guard cell membrane-associated NADPH oxidases encoded by the AtrbohD and AtrbohF genes...
  14. Zhang Y, Zhu H, Zhang Q, Li M, Yan M, Wang R, et al. Phospholipase dalpha1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis. Plant Cell. 2009;21:2357-77 pubmed publisher
    ..PA bound to recombinant Arabidopsis NADPH oxidase RbohD (respiratory burst oxidase homolog D) and RbohF...
  15. Li L, Li M, Yu L, Zhou Z, Liang X, Liu Z, et al. The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity. Cell Host Microbe. 2014;15:329-38 pubmed publisher
    ..not only positively regulates flg22-triggered calcium influx but also directly phosphorylates the NADPH oxidase RbohD at specific sites in a calcium-independent manner to enhance ROS generation...
  16. Yao Y, He R, Xie Q, Zhao X, Deng X, He J, et al. ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis. New Phytol. 2017;213:1667-1681 pubmed publisher
    ..ROS) burst in the early stages of various stresses, as a result of the lower expression level of RESPIRATORY BURST OXIDASE HOMOLOG D (RbohD)...
  17. Luo X, Xu N, Huang J, Gao F, Zou H, Boudsocq M, et al. A Lectin Receptor-Like Kinase Mediates Pattern-Triggered Salicylic Acid Signaling. Plant Physiol. 2017;174:2501-2514 pubmed publisher
    ..PAMP perception leads to calcium influx, MAPK activation, a burst of reactive oxygen species (ROS) mediated by RbohD, accumulation of the defense hormone salicylic acid (SA), and callose deposition...
  18. Xue H, Seifert G. FASCICLIN LIKE ARABINOGALACTAN PROTEIN 4 and RESPIRATORY BURST OXIDASE HOMOLOG D and F independently modulate abscisic acid signaling. Plant Signal Behav. 2015;10:e989064 pubmed publisher
    ..Moreover, the abnormally high level of reactive oxygen species in At-fla4 mutant does not depend on AtRBOHD and -F. Likewise, suppression of the At-fla4 phenotype by ABA does not depend on the 2 oxidases...
  19. Lin Z, Liebrand T, Yadeta K, Coaker G. PBL13 Is a Serine/Threonine Protein Kinase That Negatively Regulates Arabidopsis Immune Responses. Plant Physiol. 2015;169:2950-62 pubmed publisher
    ..PBL13 is able to associate with the nicotinamide adenine dinucleotide phosphate, reduced oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D (RBOHD) by split-luciferase complementation assay, and this association is disrupted ..
  20. Wang F, Chen Z, Liu X, Colmer T, Shabala L, Salih A, et al. Revealing the roles of GORK channels and NADPH oxidase in acclimation to hypoxia in Arabidopsis. J Exp Bot. 2017;68:3191-3204 pubmed publisher
    ..of GORK (depolarization-activated guard cell outward-rectifying potassium) channels and RBOHD (respiratory burst oxidase homologue D) in plant adaptive responses to hypoxia were investigated in the context of tissue specificity (..
  21. Kadota Y, Sklenar J, Derbyshire P, Stransfeld L, Asai S, Ntoukakis V, et al. Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Mol Cell. 2014;54:43-55 pubmed publisher
    ..molecular patterns (PAMPs) by surface-localized pattern recognition receptors (PRRs) activates the NADPH oxidase RBOHD by hitherto unknown mechanisms...
  22. Kalachova T, Iakovenko O, Kretinin S, Kravets V. Involvement of phospholipase D and NADPH-oxidase in salicylic acid signaling cascade. Plant Physiol Biochem. 2013;66:127-33 pubmed publisher
    ..led to phosphatidylbutanol accumulation as a result of phospholipase D (PLD) action in wild-type and NADPH-oxidase RbohD deficient plants...
  23. Rojas C, Mysore K. Glycolate oxidase is an alternative source for H2O2 production during plant defense responses and functions independently from NADPH oxidase. Plant Signal Behav. 2012;7:752-5 pubmed publisher
    ..Silencing of a gene encoding NADPH oxidase (AtRBOHD) in the gox mutants did not further increase susceptibility to a nonhost pathogen, P...
  24. Xie Y, Mao Y, Duan X, Zhou H, Lai D, Zhang Y, et al. Arabidopsis HY1-Modulated Stomatal Movement: An Integrative Hub Is Functionally Associated with ABI4 in Dehydration-Induced ABA Responsiveness. Plant Physiol. 2016;170:1699-713 pubmed publisher
    ..Moreover, the promotion of ABA-triggered up-regulation of RbohD abundance and reactive oxygen species (ROS) levels in the hy1 mutant was almost fully blocked by the mutation of ..
  25. Gupta D, Pena L, Romero Puertas M, Hernández A, Inouhe M, Sandalio L. NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity. Plant Cell Environ. 2017;40:509-526 pubmed publisher
    The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohF, which were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days...
  26. Takahashi F, Mizoguchi T, Yoshida R, Ichimura K, Shinozaki K. Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis. Mol Cell. 2011;41:649-60 pubmed publisher
    ..These findings suggest that two major activation modes in eukaryotes, Ca²+/CaMs and the MAP kinase phosphorylation cascade, converge at MPK8 to monitor or maintain an essential part of ROS homeostasis. ..
  27. Xie Y, Xu S, Han B, Wu M, Yuan X, Han Y, et al. Evidence of Arabidopsis salt acclimation induced by up-regulation of HY1 and the regulatory role of RbohD-derived reactive oxygen species synthesis. Plant J. 2011;66:280-92 pubmed publisher
    ..In atrbohD mutants, haemin pre-treatment resulted in induction of HY1 expression, but no similar response was seen in hy1-..