Gene Symbol: PRORP3
Description: proteinaceous RNase P 3
Alias: T8O5.110, T8O5_110, proteinaceous RNase P 3, proteinaceous RNase P 3
Species: thale cress
Gobert A, Gutmann B, Taschner A, Gossringer M, Holzmann J, Hartmann R, et al
. A single Arabidopsis organellar protein has RNase P activity. Nat Struct Mol Biol. 2010;17:740-4 pubmed publisher
..Finally, we show that Arabidopsis PRORP1 can replace the bacterial ribonucleoprotein RNase P in Escherichia coli cells. PRORP2 and PRORP3, two paralogs of PRORP1, are both localized in the nucleus.
Gutmann B, Gobert A, Giegé P. PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis. Genes Dev. 2012;26:1022-7 pubmed publisher
..Finally, ribonucleoprotein RNase MRP is not involved in tRNA maturation in plants. Altogether, our results indicate that ribonucleoprotein enzymes have been entirely replaced by proteins for RNase P activity in plants. ..
Pavlova L, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann R. tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences. Chembiochem. 2012;13:2270-6 pubmed publisher
Brillante N, GÃ¶ÃŸringer M, Lindenhofer D, Toth U, Rossmanith W, Hartmann R. Substrate recognition and cleavage-site selection by a single-subunit protein-only RNase P. Nucleic Acids Res. 2016;44:2323-36 pubmed publisher
..study of substrate recognition and cleavage-site selection by the nuclear single-subunit proteinaceous RNase P PRORP3 from Arabidopsis thaliana...
Walczyk D, Gösringer M, Rossmanith W, Zatsepin T, Oretskaya T, Hartmann R. Analysis of the Cleavage Mechanism by Protein-Only RNase P Using Precursor tRNA Substrates with Modifications at the Cleavage Site. J Mol Biol. 2016;428:4917-4928 pubmed publisher
..Here, we analyzed the kinetics and cleavage-site selection by PRORP3 from Arabidopsis thaliana (AtPRORP3) using precursor tRNAs (pre-tRNAs) with individual modifications at the ..
Gösringer M, Lechner M, Brillante N, Weber C, Rossmanith W, Hartmann R. Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes. Nucleic Acids Res. 2017;45:7441-7454 pubmed publisher
..The cells' viability also suggests that the essential function of the signal recognition particle can be maintained with a 5?-extended 4.5S RNA. ..