Gene Symbol: P5CS1
Description: delta1-pyrroline-5-carboxylate synthase 1
Alias: ATP5CS, T5I7.10, T5I7_10, delta1-pyrroline-5-carboxylate synthase 1, delta1-pyrroline-5-carboxylate synthase 1
Species: thale cress

Top Publications

  1. Szekely G, Abrahám E, Cséplo A, Rigó G, Zsigmond L, Csiszár J, et al. Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis. Plant J. 2008;53:11-28 pubmed
    ..Here we describe the genetic characterization of p5cs insertion mutants, which indicates that P5CS1 is required for proline accumulation under osmotic stress...
  2. Mattioli R, Marchese D, D Angeli S, Altamura M, Costantino P, Trovato M. Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis. Plant Mol Biol. 2008;66:277-88 pubmed
    ..As expected homozygous p5cs1 mutants behaved as late flowering...
  3. Mattioli R, Falasca G, Sabatini S, Altamura M, Costantino P, Trovato M. The proline biosynthetic genes P5CS1 and P5CS2 play overlapping roles in Arabidopsis flower transition but not in embryo development. Physiol Plant. 2009;137:72-85 pubmed publisher
    Overexpression of the proline biosynthetic gene P5CS1 results in early flowering in Arabidopsis...
  4. Mattioli R, Biancucci M, Lonoce C, Costantino P, Trovato M. Proline is required for male gametophyte development in Arabidopsis. BMC Plant Biol. 2012;12:236 pubmed publisher
    In crosses between the proline-deficient mutant homozygous for p5cs1 and heterozygous for p5cs2 (p5cs1 p5cs2/P5CS2), used as male, and different Arabidopsis mutants, used as females, the p5cs2 mutant allele was rarely transmitted to the ..
  5. Aleksza D, Horváth G, Sándor G, Szabados L. Proline Accumulation Is Regulated by Transcription Factors Associated with Phosphate Starvation. Plant Physiol. 2017;175:555-567 pubmed publisher
    ..i>Arabidopsis thaliana), the stress and ABA-induced Δ1-PYRROLINE-5-CARBOXYLATE SYNTHETASE1 (P5CS1) gene was previously shown to control Pro biosynthesis in such adverse conditions...
  6. Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi Shinozaki K, et al. Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant J. 1995;7:751-60 pubmed
    ..Southern blot analysis suggested that only one copy of the corresponding gene (AtP5CS) is present in A. thaliana. The deduced amino acid sequence of the P5CS protein (AtP5CS) from A...
  7. Yoshiba Y, Nanjo T, Miura S, Yamaguchi Shinozaki K, Shinozaki K. Stress-responsive and developmental regulation of Delta(1)-pyrroline-5-carboxylate synthetase 1 (P5CS1) gene expression in Arabidopsis thaliana. Biochem Biophys Res Commun. 1999;261:766-72 pubmed
    Delta(1)-Pyrroline-5-carboxylate synthetase 1 (P5CS1) is the rate-limiting enzyme in the biosynthesis of proline by Arabidopsis thaliana. Results of Northern analysis using aba1, abi1, and abi3 mutants of A...
  8. Huang D, Wu W, Abrams S, Cutler A. The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J Exp Bot. 2008;59:2991-3007 pubmed publisher
    ..These analyses demonstrate that ABA-related stress responses are modulated by other environmental and developmental factors. ..
  9. Parre E, Ghars M, Leprince A, Thiery L, Lefebvre D, Bordenave M, et al. Calcium signaling via phospholipase C is essential for proline accumulation upon ionic but not nonionic hyperosmotic stresses in Arabidopsis. Plant Physiol. 2007;144:503-12 pubmed
    ..Calcium acts as a molecular switch to trigger downstream signaling events. These results also demonstrated the specific involvement of lipid signaling pathway to discriminate between ionic and nonionic stresses. ..

More Information


  1. Kesari R, Lasky J, Villamor J, Des Marais D, Chen Y, Liu T, et al. Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation. Proc Natl Acad Sci U S A. 2012;109:9197-202 pubmed publisher
    ..identified a reduced function allele of the proline synthesis enzyme ?(1)-pyrroline-5-carboxylate synthetase1 (P5CS1) as a basis for the lower proline of Sha...
  2. Chen Q, Zheng Y, Luo L, Yang Y, Hu X, Kong X. Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana. Biochem Biophys Res Commun. 2018;495:1102-1107 pubmed publisher
    ..FRIGIDA gene in wild-type Col background (ColFRI) positively enhances the drought tolerance by activating P5CS1 expression and promoting proline accumulation during water stress...
  3. Sharma S, Villamor J, Verslues P. Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential. Plant Physiol. 2011;157:292-304 pubmed publisher
    ..in drought resistance, we analyzed Arabidopsis (Arabidopsis thaliana) ?(1)-pyrroline-5-carboxylate synthetase1 (p5cs1) mutants deficient in stress-induced Pro synthesis as well as proline dehydrogenase (pdh1) mutants blocked in Pro ..
  4. Ibragimova S, Trifonova E, Filipenko E, Shymny V. [Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana]. Genetika. 2015;51:1368-75 pubmed
    Arabidopsis thaliana delta1-pyrroline-5-carhoxylate synthase 1 gene (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv...
  5. Feng X, Li J, Qi S, Lin Q, Jin J, Hua X. Light affects salt stress-induced transcriptional memory of P5CS1 in Arabidopsis. Proc Natl Acad Sci U S A. 2016;113:E8335-E8343 pubmed publisher
    ..Primary salt stress induced the expression of Δ1-pyrroline-5-carboxylate synthetase 1 (P5CS1), encoding a proline biosynthetic enzyme and proline accumulation, which were reduced to basal level during the ..