Gene Symbol: NRPD2A
Description: nuclear RNA polymerase D2A
Alias: DEFECTIVE IN MERISTEM SILENCING 2, DMS2, DRD2, NRPD2, NRPE2, OCP1, OVEREXPRESSOR OF CATIONIC PEROXIDASE 1, nuclear RNA polymerase D2A, nuclear RNA polymerase D2A
Species: thale cress
Herr A, Jensen M, Dalmay T, Baulcombe D. RNA polymerase IV directs silencing of endogenous DNA. Science. 2005;308:118-20 pubmed
..By mutation of the two largest subunits (NRPD1a and NRPD2), we show that Pol IV silences certain transposons and repetitive DNA in a short interfering RNA pathway involving ..
Pontier D, Yahubyan G, Vega D, Bulski A, Saez Vasquez J, Hakimi M, et al
. Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis. Genes Dev. 2005;19:2030-40 pubmed publisher
..These two forms contain the same second-largest subunit (NRPD2), but differ at least by their largest subunit, termed NRPD1a and NRPD1b...
Pontes O, Li C, Costa Nunes P, Haag J, Ream T, Vitins A, et al
. The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center. Cell. 2006;126:79-92 pubmed
..We hypothesize that maintenance of the heterochromatic state involves locus-specific Pol IVa transcription followed by siRNA production and assembly of AGO4- and NRPD1b-containing silencing complexes within nucleolar processing centers. ..
Pontes O, Costa Nunes P, Vithayathil P, Pikaard C. RNA polymerase V functions in Arabidopsis interphase heterochromatin organization independently of the 24-nt siRNA-directed DNA methylation pathway. Mol Plant. 2009;2:700-710 pubmed publisher
Onodera Y, Haag J, Ream T, Costa Nunes P, Pontes O, Pikaard C. Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation. Cell. 2005;120:613-22 pubmed
..However, disruption of the Pol IV catalytic subunit genes NRPD1 or NRPD2 inhibits heterochromatin association into chromocenters, coincident with losses in cytosine methylation at ..
Kanno T, Bucher E, Daxinger L, Huettel B, Böhmdorfer G, Gregor W, et al
. A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation. Nat Genet. 2008;40:670-5 pubmed publisher
..This finding implicates a putative chromosome architectural protein that can potentially link nucleic acids in facilitating an RNAi-mediated epigenetic modification involving secondary siRNAs and spreading of DNA methylation. ..
Law J, Ausin I, Johnson L, Vashisht A, Zhu J, Wohlschlegel J, et al
. A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis. Curr Biol. 2010;20:951-6 pubmed publisher
..These results suggest that the DDR complex acts in RdDM at a step upstream of the recruitment or activation of Pol V. ..
Ream T, Haag J, Wierzbicki A, Nicora C, Norbeck A, Zhu J, et al
. Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II. Mol Cell. 2009;33:192-203 pubmed publisher
..Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense. ..
He X, Hsu Y, Pontes O, Zhu J, Lu J, Bressan R, et al
. NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation. Genes Dev. 2009;23:318-30 pubmed publisher
..V is restricted to only the two largest subunits NRPD1a/NRPD1 and NRPD1b/NRPE1 and the one second-largest subunit NRPD2a. It is unclear whether other subunits may be required for the functioning of Pol IV and Pol V in RdDM...