Gene Symbol: NRPD1B
Description: nuclear RNA polymerase D1B
Alias: ATNRPD1B, DEFECTIVE IN MERISTEM SILENCING 5, DMS5, DRD3, NRPE1, T28M21.19, T28M21_19, nuclear RNA polymerase D1B, nuclear RNA polymerase D1B
Species: thale cress

Top Publications

  1. He X, Hsu Y, Pontes O, Zhu J, Lu J, Bressan R, et al. NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation. Genes Dev. 2009;23:318-30 pubmed publisher
    ..II, our current knowledge about Pol IV and Pol V is restricted to only the two largest subunits NRPD1a/NRPD1 and NRPD1b/NRPE1 and the one second-largest subunit NRPD2a...
  2. Xie M, Ren G, Costa Nunes P, Pontes O, Yu B. A subgroup of SGS3-like proteins act redundantly in RNA-directed DNA methylation. Nucleic Acids Res. 2012;40:4422-31 pubmed publisher
    ..Thus, our results demonstrate that this group of SGS3-like proteins is an important component of RdDM. This study further enhances our understanding of the SGS3 gene family and the RdDM pathway. ..
  3. Li C, Pontes O, El Shami M, Henderson I, Bernatavichute Y, Chan S, et al. An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana. Cell. 2006;126:93-106 pubmed
    ..Here we show that AGO4 localizes to nucleolus-associated bodies along with the Pol IV subunit NRPD1b; the small nuclear RNA (snRNA) binding protein SmD3; and two markers of Cajal bodies, trimethylguanosine-capped ..
  4. El Shami M, Pontier D, Lahmy S, Braun L, Picart C, Vega D, et al. Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components. Genes Dev. 2007;21:2539-44 pubmed
    ..Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally ..
  5. Law J, Ausin I, Johnson L, Vashisht A, Zhu J, Wohlschlegel J, et al. A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis. Curr Biol. 2010;20:951-6 pubmed publisher
    ..These results suggest that the DDR complex acts in RdDM at a step upstream of the recruitment or activation of Pol V. ..
  6. Pontes O, Li C, Costa Nunes P, Haag J, Ream T, Vitins A, et al. The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center. Cell. 2006;126:79-92 pubmed
    ..We show that RDR2, DCL3, AGO4, and NRPD1b (the largest subunit of Pol IVb) colocalize with siRNAs within the nucleolus...
  7. Mosher R, Schwach F, Studholme D, Baulcombe D. PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis. Proc Natl Acad Sci U S A. 2008;105:3145-50 pubmed publisher
    DNA-dependent RNA polymerase (Pol)IV in Arabidopsis exists in two isoforms (PolIVa and PolIVb), with NRPD1a and NRPD1b as their respective largest subunits...
  8. Ream T, Haag J, Wierzbicki A, Nicora C, Norbeck A, Zhu J, et al. Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II. Mol Cell. 2009;33:192-203 pubmed publisher
    ..Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense. ..
  9. Onodera Y, Haag J, Ream T, Costa Nunes P, Pontes O, Pikaard C. Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation. Cell. 2005;120:613-22 pubmed
    ..The data suggest that Pol IV helps produce siRNAs that target de novo cytosine methylation events required for facultative heterochromatin formation and higher-order heterochromatin associations. ..

More Information


  1. He X, Hsu Y, Zhu S, Wierzbicki A, Pontes O, Pikaard C, et al. An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell. 2009;137:498-508 pubmed publisher
    ..The dual interaction of an effector protein with AGO and small RNA target transcripts may be a general feature of RNA-silencing effector complexes. ..
  2. Zhong X, Hale C, Law J, Johnson L, Feng S, Tu A, et al. DDR complex facilitates global association of RNA polymerase V to promoters and evolutionarily young transposons. Nat Struct Mol Biol. 2012;19:870-5 pubmed publisher
    ..Here, we have identified targets of Pol V in Arabidopsis thaliana on a genome-wide scale using ChIP-seq of NRPE1, the largest catalytic subunit of Pol V...
  3. Kanno T, Huettel B, Mette M, Aufsatz W, Jaligot E, Daxinger L, et al. Atypical RNA polymerase subunits required for RNA-directed DNA methylation. Nat Genet. 2005;37:761-5 pubmed
    ..DRD1 is a SNF2-like protein required for RNA-directed de novo methylation. We report here that DRD2 and DRD3 correspond to the second-largest subunit and largest subunit, respectively, of a fourth class of DNA-dependent RNA ..
  4. Pontier D, Yahubyan G, Vega D, Bulski A, Saez Vasquez J, Hakimi M, et al. Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis. Genes Dev. 2005;19:2030-40 pubmed publisher
    ..contain the same second-largest subunit (NRPD2), but differ at least by their largest subunit, termed NRPD1a and NRPD1b. Unlike NRPD1a, NRPD1b possesses a reiterated CTD, a feature that also characterizes the largest subunit of RNAPII...
  5. Eamens A, Vaistij F, Jones L. NRPD1a and NRPD1b are required to maintain post-transcriptional RNA silencing and RNA-directed DNA methylation in Arabidopsis. Plant J. 2008;55:596-606 pubmed publisher
    ..In this work, mutants defective for the NRPD1a and NRPD1b alternative largest subunits of PolIV were tested for their ability to undergo RdDM, transitive RNA silencing and ..
  6. López Sánchez A, Stassen J, Furci L, Smith L, Ton J. The role of DNA (de)methylation in immune responsiveness of Arabidopsis. Plant J. 2016;88:361-374 pubmed publisher
    ..Subsequent characterization of the hypo-methylated nrpe1 mutant, which is impaired in RNA-directed DNA methylation, and the hyper-methylated ros1 mutant, which is affected ..
  7. Zhong X, Hale C, Nguyen M, Ausin I, Groth M, Hetzel J, et al. Domains rearranged methyltransferase3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc Natl Acad Sci U S A. 2015;112:911-6 pubmed publisher
    ..This work sheds further light on the mechanism by which long noncoding RNAs facilitate RNA-directed DNA methylation. ..
  8. Dong L, Liu M, Fang Y, Zhao J, He X, Ying X, et al. DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene. Plant J. 2011;68:633-45 pubmed publisher
    ..Mutations affecting nuclear proteins DRD1 and Pol V (NRPE1 or NRPD2) relieved exo-Pdsi self-silencing, resulting in higher levels of Pdsi transcripts, which increased the ..
  9. Lahmy S, Pontier D, Cavel E, Vega D, El Shami M, Kanno T, et al. PolV(PolIVb) function in RNA-directed DNA methylation requires the conserved active site and an additional plant-specific subunit. Proc Natl Acad Sci U S A. 2009;106:941-6 pubmed publisher
    ..PolIV(PolIVa) and PolV(PolIVb), currently defined by their respective largest subunits [NRPD1(NRPD1a) and NRPE1(NRPD1b)], have been implicated in the production and activity of 24-nt small RNAs (sRNAs) in RNA-directed DNA methylation ..
  10. Han Y, Dou K, Ma Z, Zhang S, Huang H, Li L, et al. SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis. Cell Res. 2014;24:1445-65 pubmed publisher
    ..Our study suggests that the involvement of SUVR2 in transcriptional gene silencing is related to nucleosome positioning mediated by its associated chromatin-remodeling proteins. ..